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Citations for Merck :
51 - 100 of 126 citations for Phospholipase A2 Activating Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The peptides were cleaned using C18-reverse-phase ZipTipTM (Millipore, Merck KGaA). The dried peptide digest was re-suspended in 1% trifluoroacetic acid and sonicated in a water bath for 1 min before injection ...
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bioRxiv - Cell Biology 2021Quote: ... The resulting peptides were desalted with C18 ZipTip pipette tips (Merck Millipore, ZTC18S960) and loaded onto an Ultimate 3000 UPLC system (Dionex ...
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bioRxiv - Microbiology 2022Quote: ... Peptide content in each fraction was verified using QuantiPro BCA Assay Kit (Merck). Zn content was determined using PAR against a standard curve.
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bioRxiv - Developmental Biology 2022Quote: ... subsequently the peptides were eluted by 95% (v/v) acetonitrile (Merck, Darmstadt, Germany), 0.1% (v/v ...
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bioRxiv - Neuroscience 2019Quote: ... The peptides were acidified to a total concentration of 1% Formic Acid (Merck). Samples were cleaned up using OASIS sample cleanup cartridges (Waters ...
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bioRxiv - Synthetic Biology 2020Quote: ... the peptide samples were filtered through 0.45 μm filters (Merck KGaA, Darmstadt, Germany). For the digestion of rplF ...
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bioRxiv - Immunology 2021Quote: ... Proteins were eluted from beads using 40 µg of 3x FLAG peptide (Merck) for 200µL of IP sample incubated on a rotator for 45 min at 4°C.
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bioRxiv - Developmental Biology 2023Quote: ... subsequently the peptides were eluted by 95% (v/v) acetonitrile (Merck, Darmstadt, Germany), 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and the immunopurified complexes were eluted with 3X FLAG peptide (Merck Millipore/Sigma). For tandem IP experiment ...
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bioRxiv - Cell Biology 2023Quote: ... and the excess peptide was depleted via Amicon Ultra Centrifugal Filters (Merck Millipore). To label the azide-coupled nanobody with a fluorophore ...
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bioRxiv - Microbiology 2021Quote: ... and eluted in 150 µL wash buffer with 500 ng/µL FLAG peptide (Merck) by rotation with a rotator for 30 min at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... proteins were eluted with 20 μl of 1 mg/ml 3xFLAG peptide (Merck, F4799) for 15 min at room temperature with vigorous shaking ...
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bioRxiv - Immunology 2019Quote: ... HLA molecules and peptides were eluted with 1% trifluoroacetic acid (TFA, Merck, Darmstadt, Switzerland) directly into Sep-Pak tC18 100 mg Sorbent 96-well plates (Waters ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were extracted in 70% ethanol plus 5% formic acid (Merck-Millipore) twice for 20 min with permanent shaking ...
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bioRxiv - Immunology 2022Quote: ... HLA-peptide complexes were eluted by repeated addition of 0.2% TFA (trifluoroacetic acid, Merck). Eluted HLA ligands were purified by ultrafiltration using centrifugal filter units (Millipore) ...
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bioRxiv - Immunology 2020Quote: ... HLA-peptide eluates were loaded onto C18 RP-HPLC column (Chromolith Speed Rod; Merck). The bound peptides were separated using increasing concentration of 80% acetonitrile and 0.1% trifluoroacetic acid (80% ACN / 0.1% FA) ...
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bioRxiv - Microbiology 2022Quote: AB19026 ADAM10 rabbit polyclonal antibody (Immunogen: hADAM10 peptide 732-748) was purchased from Merck; anti-ADAM10 IC1427F was from R&D ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Tryptic peptides were acidified to a final concentration of 1% formic acid (FA) (Merck), cleaned up using SepPak cartridges (Waters) ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated MHC class II-peptide complexes were purified on a HisTrap HP column (Merck) and kept at -80°C until multimerization with streptavidin conjugates.
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bioRxiv - Developmental Biology 2023Quote: ... 10 µg of peptides were further desalted with C18 ZipTips (Merck Millipore, Darmstadt, Germany) to ensure complete removal of beads.
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bioRxiv - Cell Biology 2023Quote: ... The concentration of peptides was measured by a Direct Detect Spectrophotometer (Merck, Darmstadt, Germany), and solutions were made up to 100 μg in 100 μl of injection solution prior to desalting.
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bioRxiv - Cell Biology 2023Quote: ... peptide concentration was estimated via Direct Detect® Infrared Spectrometer (Merck, Bayswater, VIC, Australia), and samples were transferred to HPLC vials for proteomics analysis.
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bioRxiv - Neuroscience 2019Quote: ... Samples were then vacuum concentrated (Labconco Centrivap) and peptides purified by C18 ZipTips® (Merck) prior to analysis by LC-MS/MS or LC-SWATH-MS ...
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bioRxiv - Systems Biology 2022Quote: ... The procedure was performed by manufacturer’s instructions including peptide purification with C18 ZipTips® (Merck). The eluted peptides were snap frozen and lyophilized before reconstitution in 0.1% formic acid for HPLC-MS/MS.
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bioRxiv - Cell Biology 2023Quote: ... The peptides were desalted and fractionated using 200 µl Pierce C18 column tips (Merck Millipore).
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were then desalted using styrene divinylbenzene-reverse phase sulfonate Stage-tips (Merck, Darmstadt, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Neuroscience 2020Quote: ... The Aβ stock solution was prepared by solubilizing the Aβ25-35 peptide (Sigma-Aldrich; Merck KGaA) in sterile deionized water to a concentration of 1 mM and then incubated at 37°C for 3 days to allow self-aggregation before treatment ...
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bioRxiv - Biochemistry 2020Quote: ... Flag-tagged proteins were eluted by incubation with 150 μg/mL Flag tag peptide (Merck; F3290) with 0.1% digitonin in solubilisation buffer for 30 min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... The digested peptides were further filtered with Amicon ultra centrifugal filters (10kD MWKO, Merck Millipore, MA), dried under vacuum extensively to remove residual primary amine from reagents ...
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bioRxiv - Systems Biology 2022Quote: ... Peptide fractions were eluted using MeOH buffer containing trifluoroacetic acid (TFA) (Merck Sigma, Cat. No. 302031), FA or ABC ...
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bioRxiv - Immunology 2022Quote: ... The proteolytically cleaved peptides were extracted and desalted using ZipTip μC18 tips (Merck Millipore, Darmstadt, Germany). The peptide lysates were re-suspended in 15 µl 0.1% formic acid and analysed by nanoliquid chromatography mass spectrometry (UltiMate 3000 RSLCnano ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were eluted with 50 μl of 100 μg/mL of 3X FLAG tag peptide (Merck) in solubilization buffer followed by acetone precipitation (overnight ...
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bioRxiv - Cell Biology 2023Quote: ... pH 2.0) for endogenous MDH2 IP and incubation with 250 μg/ml 3XFLAG peptide (Merck F3290) in the case of FLAG IP ...
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miR-125-chinmo pathway regulates dietary restriction dependent enhancement of lifespan in DrosophilabioRxiv - Molecular Biology 2020Quote: ... Labelled peptides were separated on Chromolith Caprod RP-18e HR capillary column (150 × 0.1 mm; Merck Millipore) using linear gradient of buffer B (98% ACN and 0.05% TFA in water ...
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bioRxiv - Cell Biology 2020Quote: ... and proteins were eluted using 100 µl of 150 ng/µl 3× FLAG Peptide (F4799, Merck KGaA) or HA peptide (HY-P0239 ...
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bioRxiv - Microbiology 2022Quote: ... 100 µg of the dried peptides were dissolved in 90 µl of 0.2 M EPPS buffer (MERCK)/10% acetonitrile ...
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bioRxiv - Pathology 2019Quote: ... The extracted peptides were purified using C18 Zip-TIP pipette tips according to the manufacturer’s instructions (Merck Chemicals ...
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bioRxiv - Biochemistry 2019Quote: Crude peptides were purified by reverse-phase high-performance liquid chromatography using a Chromolith Prep column (Merck) on a Prominence LC-20AP system (Shimadzu ...
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bioRxiv - Biochemistry 2019Quote: Peptides were spotted at 40 µg per origin and dried on thin-layer cellulose plates from Merck. Electrophoresis was run in 1% ammonium carbonate pH 8.9 at constant 1 kV for 25 min 40,41 ...
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bioRxiv - Immunology 2020Quote: ... MHC–peptide complexes were eluted by repeated addition of 0.2% trifluoroacetic acid (TFA, Merck, Whitehouse Station, NJ). Peptides were purified by ultrafiltration using centrifugal filter units (Amicon ...
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bioRxiv - Biochemistry 2023Quote: ... the peptides were desalted and intact glycopeptides were enriched by ZIC-HILIC beads (Merck, Product no. 1.50458.0001) according to our previous study[25].
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were diluted to 50 µg/mL in 10 mM sodium acetate pH 5 (Merck, Darmstadt, Germany) and injected overflow cell two of each channel to a final signal of 3400 RU for L-CDP1 and 1300 RU for L-CDP7 ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently eluted in 150 μL of wash buffer with 500 ng/μL FLAG peptide (Merck, Darmstadt, Germany) by rotation on a rotator for 2 h at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... (final concentration of 2µg/mL) and pools of original or beta variant peptides on hydrophobic-PVDF ELISpot plates (Merck) coated with 5µg/mL anti-mouse IFNγ (AN18) ...