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Citations for Merck :
1 - 50 of 1098 citations for Parvalbumin PVALB cDNA ORF Clone Mouse N His tag since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: PC4 cDNAs wild type or carrying the W89A mutation and with a N-terminal 6x His-tag sequence were cloned into a pET-Duet1 plasmid (Merck Millipore) and transformed into competent BL21 cells (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... Immunofluorescence with anti-parvalbumin (Merck Millipore MAB1572, mouse IgG1. 1:100) and anti-Sox2 (Abcam ab92494 ...
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bioRxiv - Biophysics 2024Quote: ... and H3.1 were subcloned into a pET15b-derived plasmid containing an N-terminal 6×His tag (MGSSHHHHHHSSG) in which the thrombin cleavage site of pET15b (Merck Millipore, 69661) was replaced by the TEV protease recognition sequence ...
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bioRxiv - Biochemistry 2021Quote: ... The His6-tag fusion proteins were purified with a cOmplete™ His-tag purification resin (Roche, Merck). The fusion proteins were cleaved by incubation with thrombin protease (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Microbiology 2024Quote: ... His tags were removed using a Thrombin Cleavage Capture Kit (Merck, Darmstadt, Germany).
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-parvalbumin (1:5,000; PC255L, Merck) and rabbit anti-somatostatin (1:5,000 ...
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bioRxiv - Molecular Biology 2023Quote: ... Anti-myc-tag (clone 9E10, #05-419) was purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Cell Biology 2021Quote: ... mouse monoclonal anti-FLAG-tag (M2, #F3165, Merck), rabbit polyclonal anti-LC3B (#PA146286 ...
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bioRxiv - Immunology 2022Quote: ... The N-terminal tag was removed by an overnight incubation with thrombin (Merck). Undigested protein was removed by IMAC ...
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bioRxiv - Plant Biology 2022Quote: ... The filtered supernatant was loaded onto a column packed with cOmpleteTM His-tag purification resin (Merck). His6-proteins were eluted with 4x 2 bead volumes of elution buffer (20 mM Na HEPES ...
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bioRxiv - Biochemistry 2021Quote: ... Mouse anti-Myc tag antibody was purchased from Merck Millipore (Bangalore ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-T7-tag (Merck 69522; RRID:AB_11211744; WB 1:10000) and mouse anti-PSD-95 (Thermo Fisher Scientific MA1-046 ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Biochemistry 2021Quote: ... O-Linked N- Acetylglucosamine Antibody (ms, clone RL2, MABS157, Merck, 1:1000), AMDHD2 (ms ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-Cre (Merck Millipore clone 2D8) and mouse anti-BirA (Novus biologicals #NBP2-59939).
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bioRxiv - Biophysics 2024Quote: ... clone 4G10 (Mouse Monoclonal) was from Merck Millipore (Darmstadt ...
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bioRxiv - Neuroscience 2021Quote: ... anti-n-terminal APP (1:1000, mouse, 22c11, Merck), anti-c-terminal APP (1:1000 ...
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bioRxiv - Microbiology 2024Quote: ... Mouse mAb anti-HA (clone HA-7) and mouse anti-tubulin (clone B5-1-12) were purchased from Merck. Mouse mAb anti-V5 (SV5-PK1 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein purification steps were analyzed by 12.5% SDS-PAGE and WB with the His-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Germany). Protein concentration was determined using the Bradford method (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... and mouse anti-GAPDH (clone 6C5, Merck Millipore). Secondary antibodies were goat anti-mouse AF488 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-NeuN (clone A60, Merck, Darmstadt, Germany) (Table 3) ...
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bioRxiv - Neuroscience 2021Quote: ... mouse anti-NeuN (clone A60, Merck, Darmstadt, Germany); mouse anti-CD79a (ThermoFisher) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... clone 4E6.2 (1:1000; mouse monoclonal, Merck, MAB3067), anti-Nav1.5 (1:200 ...
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bioRxiv - Molecular Biology 2021Quote: ... anti-DNA:RNA hybrid clone S9.6 (mouse, MABE1095 [Merck Millipore] ...
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bioRxiv - Microbiology 2024Quote: ... mouse monoclonal anti-AGE antibody (clone 3D11, Merck) and mouse monoclonal Anti-methylglyoxal (clone 9F11 ...
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bioRxiv - Microbiology 2024Quote: ... and mouse monoclonal Anti-methylglyoxal (clone 9F11, Merck), which were used at 1/200.
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-APP N-terminal 1:1000 (22C11, #MAB348, Merck), rabbit anti-APP C-terminal 1:1000 (#A8717 ...
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bioRxiv - Neuroscience 2024Quote: ... mouse anti-APP N-terminal 1:2000 (22C11, #MAB348, Merck) to visualize dystrophic neurites ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-Calbindin1 (clone CB-955, Merck, Darmstadt, Germany); mouse anti-NeuN (clone A60 ...
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bioRxiv - Biochemistry 2023Quote: Mouse monoclonal anti-γ-tubulin (clone GTU-88; Merck) at a dilution of 1:1000 was used for the γ-tubulin western blot in Supplementary Figure 1B ...
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bioRxiv - Microbiology 2024Quote: ... mouse mAb anti-HA (clone HA-7)(Merck, H3663) or 2 µg mouse IgG2a kappa isotype control (clone eBM2a ...
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bioRxiv - Molecular Biology 2024Quote: ... clones were transferred to liquid Brock medium (0.1% (w/v) N-Z-amine (Merck, Germany) and 0.2% (w/v ...
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bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
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bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
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bioRxiv - Plant Biology 2023Quote: ... the sequence of CBL1 was fused with an N-terminal 2xStrepII tag in the pET-24b vector (Merck, Darmstadt, Germany). Sequences of active CPK5 and CIPK26 were fused with an N-terminal 2xStrepII tag in the pIVEX-1.3WG vector (Biotechrabbit ...
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bioRxiv - Biophysics 2024Quote: The gene encoding human GRB2 in addition to a N-terminal His6 tag with a tobacco etch virus (TEV) protease cleavage site was constructed into the vector pET14b (Merck) for over-expression in the Escherichia coli BL21 (DE3 ...
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bioRxiv - Microbiology 2023Quote: N,N,N’,N’-tetrakis-(2-pyridylmethyl)ethylenediamine (TPEN) (Merck, 616394), made up in DMSO and used at 5 µM ...