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Citations for Merck :
201 - 246 of 246 citations for PDGF Receptor Substrate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Neuroscience 2023Quote: ... after which the blot was developed with the ECL chemiluminescent reagent (WBKLS0500, Immobilon Western Chemiluminescent HRP Substrate, MERCK, USA). We obtained an intense band at ∼40kD and a very faint one at ∼32kD (Supplementary Figure 1(c) ...
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bioRxiv - Cell Biology 2023Quote: ... The immunoreactive bands were detected by using enhanced chemiluminescence (ECL) reagent (Immobilon Crescendo Western HRP substrate; Merck Millipore, DE). Band densities were quantified by densitometry using Image LabTM Version 6.0.1 2017 (Bio-Rad Laboratories ...
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bioRxiv - Immunology 2023Quote: ... The signal was revealed with 3,3’-Diaminobenzidine (DAB) substrate (Palex) and the nuclei were counterstained with Mayer hematoxylin (Merck). The incubations were carried out in humid chambers to prevent the samples from drying out ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated in 5 mM Tris-HCl buffer (pH 7.5) containing each substrate (1% glucomannan, Neogen, MI, USA; 1% polygalacturonic acid, Neogen; 1% carboxymethyl cellulose, Merck; 1% soluble starch ...
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bioRxiv - Cell Biology 2023Quote: ... Detection by enhanced chemiluminescence was performed using the Immobilon Forte HRP Western substrate (ref WBLUF0500, Merck Millipore, Darmstadt, Germany) or SuperSignal West Femto Maximum Sensitivity Substrate (ref 34096 ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibodies were detected with HRP-conjugated anti-rabbit or anti-mouse IgG and visualised with Immobilon Western HRP substrate (Merck).
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bioRxiv - Genetics 2021Quote: ... Blots were washed thrice with TBS-T and visualized on a LAS4000 Fuji imaging system after incubating with Immobilon Western Chemiluminescent HRP substrate (Merck). The following antibodies were used ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Molecular Biology 2022Quote: CobB deacetylase activity was measured as described earlier (Heltweg et al., 2005, 2003) as deacetylation of MAL substrate (Sigma/Merck), an artificial fluorogenic substrate for Zn2+ and NAD+ -dependent deacetylases ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# AS1107) for one hour at room temperature and visualized using the Immobilon ECL substrate kit (Millipore, Merck KgaA, Germany). Primary antibodies specific to PLLP (1:700 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature (RT) and revealed by chemiluminescence using Immobilon Crescendo or Forte Western HRP substrate (Millipore Merck).
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bioRxiv - Microbiology 2020Quote: ... the membranes were incubated with the respective HRP-conjugated secondary antibody in 5% dry milk powder in TBS-T for 1h at room temperature washed three times in TBS-T and imaged on an ECL ChemoCam 3.2 Imager (Intas) using Immobilon Forte Western HRP substrate (Merck Millipore). For pulldown of gH-V5 or gH-V5/ gL-Flag complexes ...
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bioRxiv - Microbiology 2022Quote: ... Hydrolysis of 44 soluble non-chromogenic monoester substrates (Table S2) and T-2 mycotoxin (Merck Life Science S.L.U., Madrid, Spain) was assayed at 37ºC using a pH indicator assay with Phenol Red and monitored at 550 nm (46) ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 3x with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) using a Gel-Doc XR+ System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2023Quote: ... 4.0 % (v/v) formaldehyde) and stained for beta-galactosidase activity using Red-Gal or X-Gal substrates (RES1364C-A103X or B4252, Merck) to identify the injected side and correctly targeted embryos ...
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bioRxiv - Biochemistry 2023Quote: ... The reactions were stopped by separating the enzyme and soluble products from the insoluble chitin substrate by filtration using 96-well filter plates (Merck). The concentration of oxidized LPMO products was also determined in aliquots taken from the pre-incubation reaction with β-chitin to allow accounting for carry-over of products potentially formed in the pre-incubation phase.
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bioRxiv - Microbiology 2023Quote: ... Wells were again washed three times with PBS and bound alkaline phosphatase quantified using a p-Nitrophenol phosphate substrate solution for ELISA (Merck) and A405 readings.
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed with three rounds of PBS before incubation in 200 µl p-Nitrophenol phosphate substrate solution for ELISA (Merck) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: ... bound secondary antibodies were detected using either EZ-Chemiluminescence Reagent (Geneflow Ltd) or Luminata™ Forte Western HRP Substrate (Merck Millipore) and a Biospectrum 500 imaging system (UVP ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were washed with TBST five times for 5 min each and visualized using a chemiluminescence detection system (Immobilon Western HRP Substrate, Merck Millipore). All steps were carried out with phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2019Quote: ... The membranes were finally washed again 4 times for 10 min in TBS and revealed with Immobilon Western Blotting Chemoluminescence HRP substrate (Merck Millipore) and Super RX-film (Fujifilm ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate, Merck Millipore). Digital images were acquired on a ChemiDoc XRS System (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... and detected using primary and corresponding HRP-conjugated secondary antibodies on Fusion SL imaging system (Vibler) using Immobilon Western Chemiluminescent HRP Substrate (Merck, WBKLS0500). Molecular size of bands is marked in each panel [kDa] ...
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bioRxiv - Cancer Biology 2022Quote: ... Membranes were then incubated for 1 hour with a species-appropriate HRP-conjugated secondary antibody (diluted 1:5000 in TBST) before bands were visualised using Immobilon Forte Western HRP Substrate (Merck Millipore). Primary antibodies used were mouse anti-αSMA (Dako ...
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bioRxiv - Developmental Biology 2020Quote: ... membranes were washed 3 times with TBS-T and subjected to chemiluminescence detection with Immobilon Western Chemiluminescent HRP (Horseradish Peroxidase) Substrate (Merck Millipore) using a Gel-Doc XR+ System (BioRad) ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were incubated with goat anti-mouse horse radish peroxidase (HRP)-conjugated secondary antibody and the bands developed with Luminata Forte Western HRP Substrate (Merck Millipore).
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bioRxiv - Developmental Biology 2022Quote: ... The membranes were washed 3x in TBST for 10 min again and afterwards incubated in Immobilon ECL Ultra Western HRP Substrate (Merck, WBULS0100) for 2-5 min and scanned with GS-800 densitometer (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... the membranes were washed three times with TBST for 10 min and proteins were detected via chemiluminescence using Immobilon® Forte Western HRP substrate (Merck). For the multiplex detection of native LpxC and the LPS level 12% stain-free FastCast Acrylamide gels (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed four times for 5 minutes in PBST and incubated with Immobilon® Forte Western HRP Substrate (Merck, WBLUF0100) for 3 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
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bioRxiv - Cancer Biology 2023Quote: ... The signal was visualized using appropriate primary and secondary antibodies and the Immobilon™ Western Chemiluminescent HRP substrate (Merck, Darmstadt, Germany) and developed on an X-ray film ...
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bioRxiv - Molecular Biology 2020Quote: ... CsCl fractions containing needle complexes and the substrate were pooled and incubated with Anti-FLAG M2 magnetic beads (M8823, Sigma Aldrich/Merck KGaA) prewashed in FR3 buffer (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... rinsed with PBS and the proteins were visualized with Immobilon Western Chemiluminiscent HRP substrate (Merck/Millipore, used at 1:1:3 (H2O) dilution ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Immunoreactive bands were detected using iBright CL1000 chemiluminator or LAS 3000 (Fujifilm, Tokyo, Japan) with an enhanced chemiluminescent substrate for the detection of horseradish peroxidase (HRP; Merck, Darmstadt, Germany). The intensity of the RAS band was quantified using ImageJ software and normalized to the intensity of the GAPDH band.
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bioRxiv - Microbiology 2023Quote: ... 50 µl of each dilution were mixed with 10 µl of 0.4 mg ml-1 4-methyl-umbelliferyl-β-D- galactopyranoside (MUG) substrate (Merck, Darmstadt, Germany) that was prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2024Quote: ... IHC staining was performed using Ki67 and cleaved caspase 3 antibodies and the 3,3’-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride (Merck Millipore, Darmstadt, Germany) as described previously30.
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bioRxiv - Physiology 2021Quote: ... All incubations with primary antibodies were performed for 16 hours at 4°C. Blots were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).
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bioRxiv - Molecular Biology 2021Quote: ... The development was performed in an ImageQuant™ LAS 500 Ge Life Sciences apparatus (Piscataway, NJ, USA) using the appropriate substrate of peroxidase (Luminata™Forte, Merck Millipore). The reaction was conducted in the dark ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Genetics 2022Quote: ... Incubation with appropriate secondary HRP-labelled antibody (less than 1h) was followed by detection with Immobilon western chemiluminescent HRP substrate (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Protein signal was developed using enhanced chemiluminescence according to the manufacturer’s instruction (Immobilon® Forte Western HRP substrate, Merck Millipore Ltd., VIC, Australia) and visualised using the ChemiDocTM XRS system (Bio-Rad ...
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bioRxiv - Cancer Biology 2019Quote: ... the plasmid construct of β-galactosidase was co-expressed in equal amounts in wells and expression was measured in the presence of the ONPG substrate (Merck, 369-07-3). The reaction was stopped with 1M Tris-Cl (pH 11 ...
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bioRxiv - Cell Biology 2023Quote: ... TBS-T was used for washing the membranes. Membranes were developed using SuperSignal West Pico (Pierce, Ref. 34580) or Luminata Forte Western HRP substrate (Merck Millipore, Ref. WBLUF0500).
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times with 200 µl/well of PBS/Tween and incubated for 10 min at room temperature with 50 µl/well of 3,3‘,5‘,5‘-tetramethylbenzidine (TMB)/H2O2 peroxidase substrate (Merck KGaA, cat. no. T4444). The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... The two aqueous streams were supplied with the cell solution and with a 3 μM substrate solution containing lysis agents (0.7× BugBuster protein extraction reagent, Merck Millipore; 60 kU/mL rLysozyme, Novagen) in droplet assay buffer ...