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Citations for Merck :
401 - 450 of 454 citations for Okadaic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Microbiology 2020Quote: ... A was 1 mM ammonium formate pH 9 (for lincomycin detection, prepared by titration of formic acid 98–100%, Merck, Germany with ammonium hydroxide 28–30% ...
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bioRxiv - Immunology 2021Quote: ... neuraminidase-specific inhibitor DANA was added to a final concentration of 100 μM (N-Acetyl-2,3-dehydro-2-deoxyneuraminic acid, Merck, USA). It was used for 48 h prior stimulus.
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Bioengineering 2022Quote: ... Nitric acid 65%, N,N-dimethylformamide (DMF), sodium hydroxide (NaOH) and Ethylenediamine (EDA, ≥99 %) were purchased from Merck (Darmstadt, Germany). Dulbecco’s modified Eagle’s medium ...
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bioRxiv - Cell Biology 2022Quote: ... 0.05 M cacodylate buffer (using dimethylarsinic acid sodium salt trihydrate from Merck and HCl for pH adjustment to 6.4-7.4) at room temperature in a glass staining block ...
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bioRxiv - Molecular Biology 2023Quote: ... To deplete Xrn1 and Dcp2 with the AID tags (Nishimura et al., 2009; Morawska and Ulrich, 2013) 1-Naphthaleneacetic acid (1-NAA; N0640-25G, Merck) was added to a final concentration of 1 mM ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Neuroscience 2023Quote: ... before transcardially perfusing with 21-28 ml of ice-cold PBS and 4%PFA-0,12% picric acid (Merck, Søborg, Denmark). Spleens were excised and weighed ...
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bioRxiv - Microbiology 2023Quote: ... the pH of the TSB was adjusted with hydrochloric acid solution (1M and 6 M HCL; Merck KGaA, Darmstadt, Germany) immediately prior to the experiment ...
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bioRxiv - Biophysics 2024Quote: Glass coverslips (Paul Marienfeld GmbH, 24 x 50 mm, 170 ± 5 μm) were overnight incubated in 100 mM Sulphuric acid (Merck). Afterwards the coverslips were rinsed consecutively with Milli-Q water ...
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bioRxiv - Systems Biology 2023Quote: ... UK), eosin Y 1% alcoholic and xylene (CellPath, UK), periodic-acid (TCS Biosciences Ltd, UK) and Schiff’s reagent (Merck, Germany). Description of the IF and IHC antibodies used can be found in the Supporting Information.
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bioRxiv - Biochemistry 2023Quote: ... containing labeled or unlabeled glucose/glutamine and quenched by adding 50% ice-cold methanol containing 2 µg/mL cinnamic acid (Merck) as an internal control ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the MSA agar plate was flooded and 500 µg/ml of nalidixic acid (Himedia) and 1 mg/ml of apramycin (Merck). After incubation ...
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bioRxiv - Neuroscience 2024Quote: ... 9-β-D-Ribofuranosyladenine (adenosine) and (S)-(+)-a-amino-4-carboxy-2-methylbenzeneacetic acid (LY-367385) were purchased from Merck.
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bioRxiv - Cancer Biology 2024Quote: ... cells were washed with 1X PBS and fixed with ice-cold 10% trichloroacetic acid (Sigma Aldrich; Merck cat. no: 27242) at dark ...
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bioRxiv - Microbiology 2024Quote: ... Samples were separated on a 1.2 % agarose gel stained with 0.01 % (v/v) GelGreen Nucleid Acid Stain (Merck, Darmstadt, Germany) in TBE buffer for 2 h at 120 V ...
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bioRxiv - Immunology 2024Quote: ... The ethanol and unloaded nucleic acids in the mixtures were removed using Amicon Ultra centrifugal filters (10- or 30-kD MWCO, Merck) for two rounds with 5- and 15-times volumes of 1× PBS ...
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bioRxiv - Genomics 2019Quote: ... heads of 10 ISO-1 females were chopped in 500 µL of ice-cold Otto 1 Buffer (0.1 mol/L citric acid, 0.5% Tween20 (Merck KGaA, Darmstadt, Germany)) ...
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bioRxiv - Biophysics 2022Quote: ... three washes in PBS+10%HS each lasting 5min and staining of nucleic acids with Hoechst 33342 (5μg/mL-Merck Bio-sciences) for 5min ...
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bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
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bioRxiv - Molecular Biology 2019Quote: To evaluate microscopic features of the captured cells such as morphological types and possible alterations we then used another collection membrane directly stained with hematoxylin and eosin after fixation for 10 min in a fixation solution (100 mL of 70% ethanol, 5 mL of glacial acetic acid and 5 mL of 37% formaldehyde solution – all solutions are from Merck Millipore). Samples were hydrated with distilled water for 5 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... tumefaciens were resuspended in 50 mM MES (2-Morpholinoethanesulfonic acid hydrate) (Duchefa, Haarlem, The Netherlands) - KOH buffer (pH 5.6) containing 2mM NaH2PO4 (Merck, Darmstadt, Germany), 100 µM acetosyringone (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: The pure protein recovered after HPLC purification in 1% acetic acid was concentrated in Amicon Ultra-15 (cut-off 3 kDa) centrifugal filter units (Merck Millipore) to reach the final protein concentration of 1 mM ...
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bioRxiv - Microbiology 2020Quote: ... Samples were then resuspended in 10 μl of 0.1% trifluoroacetic acid and tryptic peptides were desalted using a Zip-Tip® column (Merck Millipore) pipette tip according to the manufacturer’s protocol and dried in a vacuum concentrator before analysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS).
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs of mouse BRME1 (amino acid residues: 540-578 and 540-574) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6 tag ...
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bioRxiv - Systems Biology 2024Quote: ... The solvent system comprised solvent A consisting of 0.1% formic acid in Milli-Q water (both HPLC grade, Merck, Darmstadt, Germany) and solvent B being methanol (UHPLC grade ...
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bioRxiv - Microbiology 2023Quote: ... Samples were passed through a 0.2 µm nylon membrane prior to analysis with liquid chromatography as described previously 50. Separation for organic acid quantification (i.e. SCFAs and intermediate metabolites) was conducted on a LaChrom HPLC-System (Merck-Hitachi, Japan) using a SecurityGuard Cartridges Carbo-H (4 × 3.0 mm ...
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bioRxiv - Cancer Biology 2024Quote: ... was followed by 5 minutes of rinsing with water and 20-30 seconds of Eosin solution (Merck, 115935, 100ml supplemented with one drop of acetic acid, Merck, 1.00063). For PAS staining ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells were maintained in RPMI-1640 medium supplemented with 4.5 g/L glucose, 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, 1.0 mM sodium pyruvate (Sigma-Aldrich; Merck KGaA), 10% fetal bovine serum (Hyclone ...
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bioRxiv - Plant Biology 2021Quote: ... Extracted peptides were dried in a speed-vac and resuspended in (v/v) 2% acetonitrile/0.2% trifluoroacetic acid (Merck, catalog no. 302031). A total of four biological replicates for each sample type was submitted.
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Synthetic Biology 2022Quote: ... All yeast transformations were plated on synthetic complete (SC) plates containing 1.7 g/L yeast nitrogen base without amino acids and ammonium sulphate (Sigma Aldrich, Merck Life Science), 1 g/L monosodium glutamate (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Protein transfer was then visualized using 0.1 % Ponceau S solution (w/v) in 5% acetic acid (Merck Life Sciences, Cat #: P7170-1L) and imaged ...
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bioRxiv - Molecular Biology 2024Quote: The frozen brain tissue samples were first thawed on ice and homogenized in 25 volumes (w/v) of ice-cold 0.1 M perchloric acid (Merck KGaA, Darmstadt, Germany) using an ultrasound homogenizer (Soniprep 150 ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were loaded onto the nanoACQUITY UPLC Trapping Column (Waters Corporation, Milford, MA, USA) using a mobile phase consisting of water containing 0.1% formic acid (Merck KGaA, Darmstadt, Germany). Subsequently ...
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bioRxiv - Synthetic Biology 2023Quote: ... VpALDH and SlAlaR were combined (each at 0.05 μM) with 1 U mL−1 D-amino acid oxidase (DAAO) from porcine kidney (Merck product no. A5222) and 2 U mL−1 catalase from bovine liver (Merck-MilliPoreSigma ...
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bioRxiv - Cancer Biology 2024Quote: ... colonies from MDA-MB-468 cells were fixed by replacing the medium with a solution of 3% trichloroacetic acid in water (Merck, #T6399-500G), rinsed twice with water ...
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bioRxiv - Biophysics 2019Quote: ... OH, USA). Ethanol (95%) was purchased from Aik Moh (Singapore), hydrochloric acid (HCl, catalog no. 100317) was purchased from Merck (Burlington, MA, USA) and fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Genetics 2022Quote: ... 5 µg SA8 was dissolved in 10 µL 10 mM hydrochloric acid and digested with 20 ng/µL pepsin (Merck, cat. No. 10108057001) at 37 °C for 6 h ...
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bioRxiv - Microbiology 2023Quote: ... The protocol was similar to the other shake flask cultivations using a starting OD of 20 but fully 13C labeled glycolic acid was used (Merck product no. 604011) for all 13C cultures and unlabeled glycolic acid with a natural isotopologe distribution for all natC cultures ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μl of cell suspension was collected and mixed with 200 μl of ice-cold HBSS containing 5% fatty acid-free bovine serum albumin (BSA, Merck Millipore, 126575-10GM). To quench the fluorescence of non-internalized NBD-phospholipids ...