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Citations for Merck :
651 - 700 of 956 citations for Mouse Serpin B10 SERPINB10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... and mouse BRCA2 (amino acid residues: 2232-2283) were cloned into pMAT11 (Peranen et al., 1996) and pRSF-Duet1 (Merck Millipore) vectors for expression with an N-terminal TEV-cleavable His6-MBP tag and an N-terminal TEV-cleavable MBP tag ...
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bioRxiv - Cancer Biology 2023Quote: ... Resulting immunocomplexes were detected by HRP-conjugated anti-mouse IgG or anti-rabbit IgG secondary antibodies and enhanced chemiluminescent (ECL) reagents (WBLUF0100, Merck Millipore).
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bioRxiv - Cell Biology 2020Quote: ... and subsequent ligation (Rapid DNA Ligation Kit, Roche, Merck, Zug, CH). Then ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Polyvinylidine difluoride membrane and ECL kit (WBKLS0500) were purchased from Merck Millipore (Billerica ...
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bioRxiv - Genetics 2020Quote: ... Chromatin immunoprecipitation was performed using Agarose ChIP Assay Kit (Merck Millipore). Briefly,107 cells were lysed by incubation on ice with 130 µl lysis buffer for 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: Chromatin immunoprecipitation assay was performed using a commercial kit (Merck-Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Immunoreactive bands were detected using ECL detection reagent kit (Merck-Millipore) and the FusionSL chemiluminescence documentation system (Vilber-Lourmat) ...
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bioRxiv - Cell Biology 2022Quote: ... H&E staining was carried out using a kit (Merck, Germany). Muscle fiber cross sectional area was measured with the ImageJ 1.48 software (National Institutes of Health ...
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bioRxiv - Cell Biology 2022Quote: Following cytospin of leukocytes the Hemacolor® staining kit (Merck, Germany) that works based on eosin (red)/azur (blue ...
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bioRxiv - Neuroscience 2021Quote: ... and antibodies were diluted with SignalBoost™Immunoreaction Enhancer Kit (Merck). Primary antibodies included phospho-TDP-43 (Ser409/410 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Collagen was detected with the Van Gieson kit (Merck, Darmstadt, Germany), and reticulin ...
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bioRxiv - Biochemistry 2020Quote: The PKH26 Fluorescent Cell Linker Mini Kit was purchased from Merck-Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... and stained using the AldeRed ALDH detection assay kit (Merck SRC150) according to manufacturer specifications.
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bioRxiv - Genomics 2022Quote: ... Keratanase (G6920-5UN)) and a glycoprotein deglycosylation kit (Merck catalog #362280). We then used Water-18O (97% atom ...
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bioRxiv - Neuroscience 2023Quote: ... Congo Red staining was performed using Congo Red Staining Kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: BACE-1 activity was assessed using a fluorescent assay kit (Merck), while plasma glycerol and plasma HDL-C were determined using colorimetric kits (Sigma) ...
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bioRxiv - Neuroscience 2022Quote: ... and the bound IgG was eluted using Ab Buffer Kit (Merck), and then the eluate was dialyzed overnight at 4°C in PBS using a 10 kDa dialysis membrane (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
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bioRxiv - Microbiology 2023Quote: ... the H-Tag Antibody HRP Conjugate Kit (Merck-Millipore, Darmstadt, Germany) was used according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: ... the ApopTag® Red In Situ Apoptosis Detection Kit (Merck S7165) was used ...
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bioRxiv - Cell Biology 2022Quote: ... and bands were detected using chemiluminescence detection kit (Merck Millipore; WBKLS0050).
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bioRxiv - Genomics 2023Quote: ... The Sigma Spectrum plant total RNA kit (Merck KGaA, Darmstadt, Germany) was used for RNA extraction that followed the manufacturer procedure ...
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bioRxiv - Genetics 2023Quote: ... using the Spectrum Plant Total RNA kit (Merck KGaA, Darmstadt, Germany). Gene expression was determined by RT-qPCR as previously described (Zenoni et al. ...
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bioRxiv - Physiology 2023Quote: ... glucose levels were determined using a Glucose (GO) assay kit (Merck). Blood insulin concentrations were measured by Rat Insulin ELISA kit (Mercodia) ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Soluble proteins were then dosed using the Bicinchoninic Acid Kit (Merck) and diluted in Laemmli protein sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: For immunocytochemical staining we used the following primary and secondary antibodies: mouse anti-NFL antibody clone DA2 (Merck Millipore, cat. no. MAB1615), mouse anti-NFL antibody clone F-12 (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunofluorescence staining of the DNA double-strand-break marker γH2AX [32] was performed using a JBW301 mouse monoclonal anti-γH2AX antibody (Merck, Darmstadt, Germany) and an Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Iba1 1:1000 (Wako Chemicals GmbH, Neuss) or double-stained with goat anti-td-Tomato and mouse anti-NeuN 1:500 (Merck Millipore, Darmstadt). Counter-staining for nuclei with 42,6-diamidin-2-phenylindol (DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
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bioRxiv - Plant Biology 2021Quote: ... the sections were incubated in 1× PBS containing 1% (1:100 dilution) mouse monoclonal anti-Flag M2 primary antibody (Merck, cat. no. F1804) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies used to image centrioles in expanded RPE-1 cells were rabbit anti-polyglutamate chain (polyE), pAb (IN105, 1:250) and mouse monoclonal anti-acetylated tubulin (Merck, T7451, 1:500). Secondary antibodies were goat anti-Rabbit Alexa488 (Thermo-Fisher Scientific ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...