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Citations for Merck :
151 - 200 of 2817 citations for Mouse G Protein Coupled Receptor Family C Group 6 Member A GPRC6A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Plant Biology 2023Quote: ... then 200 µl of each sample was digested for 16 h at 37 °C with α-amyloglucosidase (6 U/ml, Merck), and α-amylase (4 U/ml ...
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bioRxiv - Immunology 2022Quote: ... Protein was digested by incubating membrane at 50°C for 1 h with proteinase K (Merck) diluted in PK-SDS buffer ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Biochemistry 2020Quote: ... Portions of 10 g cell pellet were resuspended in 50 mL Bugbuster (BugBuster® Protein Extraction Reagent, Merck Millipore) supplemented with 5 µL Benzonase Nuclease (Novagen ...
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bioRxiv - Biochemistry 2022Quote: ... Immobilized pepsin was removed by centrifugation at 1,000 x g for 30 sec at 0 °C using a 0.65 µm filter inlet (Merck Millipore, Darmstadt, Germany) and the flow through was injected immediately into the HPLC-MS/MS system ...
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bioRxiv - Plant Biology 2021Quote: ... were moved to new tubes and concentrated at 14,000×g for 10 min at 4 °C using Amicon ultra-0.5 ml centrifugal filters (catalog number Z677094, Merck millipore, Sweden). Thylakoid ...
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bioRxiv - Immunology 2020Quote: ... samples were centrifuged at 22000 x g for 20 minutes at 4 °C and the supernatant was filtered through Millex GV 220 nm filter (Merck-Millipore). For acid treatment acetic acid was used with 0.1 N final concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were centrifuged (1500 g for 5 min at 4°C) and resuspended in 3 x PCV buffer A + 0.1% NP40 (Merck Life Science). After 10 further min on ice ...
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bioRxiv - Immunology 2021Quote: ... After digestion for 2 min in a water ice bath the samples were separated by centrifugation at 1000 x g for 30 s at 0°C using a 22 µm filter (Merck, Millipore) and were immediately analyzed by LC-MS ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were first concentrated by centrifugation at 4,000 x g at 21°C using Amicon® Ultra-4 Centrifugal Filter Unit with 100kDa molecular weight cutoff (UFC810024, Merck Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... with PBS pH 7.4 for 10 min at 4400 x g and 4°C using an Amicon Ultra-4 concentrator with 3 kDa cutoff (Merck Millipore). The degree of labelling (DOL = 1.96 ...
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bioRxiv - Neuroscience 2022Quote: ... Unencapsulated molecules were removed by washing at least 4 times the LPs suspensions with PBS pH 7.4 for 20 min at 4°C and 5000 x g using an Amicon Ultra-4 concentrator with 100 kDa cutoff (Merck Millipore). The degree of encapsulation was calculated by subtracting from the total mass of the molecule to be encapsulated that present in the flow-through and was found to be higher than 75% in all LP preparations.
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bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
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bioRxiv - Cancer Biology 2021Quote: Chromatin was isolated from 20 million cells using the Magna ChIP A/G Kit (One-day chromatin Immunoprecipitation Kits, Merck) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Na2HPO4 (7.2 g) and KH2PO4 (1.2 g) procured from Merck as per Cold Spring Harbor protocols.
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bioRxiv - Biophysics 2022Quote: ... NaCl (40 g) and KCl (1 g) procured from Merck.
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bioRxiv - Cell Biology 2020Quote: ... we imaged cells in the CellASIC ONIX microfluidic device (Merck Group, Darmstadt, Germany). For flow cytometry and snapshot images ...
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bioRxiv - Immunology 2020Quote: ... This was followed by incubation with anti-goat IgG-FITC (Merck Group, Germany) and anti-AKT-AlexaFluor647 (clone 5G3 ...
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bioRxiv - Cell Biology 2022Quote: ... Experiments with Poly(sodium) 4-styrenesulfate (PssNa) (243051; Sigma-Aldrich/Merck group, USA), a polyelectrolyte able to scavenge charged molecule 39 ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 5-10% fetal bovine serum (F7524; Sigma-Aldrich/Merck group, USA) and 1% penicillin/streptomycin (15140122 ...
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bioRxiv - Microbiology 2022Quote: ... Sprayers containing either spore suspensions or sterile Milli-Q water (Merck Group, Switzerland) were used ...
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bioRxiv - Microbiology 2022Quote: ... A DNA fragment containing group II intron and ltrA gene from pJIR750ai (Merck) as well as a Clostridium-E ...
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bioRxiv - Biophysics 2023Quote: ... Unreacted aldehyde groups were quenched using 0.1% NaBH4 (#71320, Sigma Aldrich now Merck), for 7 minutes in PBS ...
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bioRxiv - Systems Biology 2020Quote: FGF receptor inhibitor (FGFRi) stock: PD 173074 (#P2499-5MG, Merck & Co., Inc., NJ, USA) diluted in DMSO (#D2650-5×5ML ...
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bioRxiv - Biochemistry 2021Quote: ... 50 µg of protein solution was mixed with dye at a three-fold molar excess in the presence of NaHCO3 (Merck) buffer at pH 8.3 ...
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bioRxiv - Cell Biology 2023Quote: ... Total IgG were isolated from post-immune sera by affinity purification on Sepharose-protein G (Merck, Cytiva 17-0618-05). Custom sheep pAb anti-pS55 and anti-pS44 NDC80 pAb was raised by the Scottish Blood Transfusion Service using a 4-month long program ...
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bioRxiv - Physiology 2023Quote: ... and 20 µg protein extracts were separated with 10-15% SDS-PAGE gel and transferred to the PVDF membranes (Millipore ISEQ00010, Merck). Each membrane was blocked and slit into two parts ...
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bioRxiv - Biochemistry 2023Quote: ... 40 μg of protein was added to each well and run in Boric acid-Tris buffer (192 mM Boric acid, Merck; 1 mM EDTA ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Genetics 2023Quote: ... after adding 2 g activated (i.e., baked at 300°C for 2h) molecular sieves (5Å, 45-60 mesh size, Merck, KGaA, Darmstadt, Germany). The molecular sieves were filtered out by loading the extract into a glass funnel (50 mm inner diameter ...
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bioRxiv - Bioengineering 2024Quote: ... was coupled with G6-OH using PyBOP (Merck) as a coupling reagent to produce an intermediate with protecting group Fmoc ...
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bioRxiv - Neuroscience 2019Quote: ... AB5543), chicken anti-β3 tubulin (1:500, AB9354) and mouse anti-ankyrin G (1:500, MABN466) were purchased from Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Evolutionary Biology 2023Quote: ... CDK9 was immunoprecipitated with 15μg of an anti-CDK9 antibody (D-7, sc-13130, lot no # B1422) or control mouse IgG (12-371, Merck) in IP Buffer with 2X SDS buffer (100mM NaCl ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... c-Src was detected using anti-Src (GD11 clone) antibody (1:200, Merck Millipore, Mouse, 05-184). Phosphorylated c-Src was assessed using anti-phospho-Src Tyr 418 antibody (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... 1:600-diluted rabbit anti-CENP-C (#554),55 mouse anti-HP1α (1:1,000, #MAB3584, Merck Millipore), rabbit anti-HP1β (1:800 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with primary antibodies against puromycin (mouse monoclonal 1:500, Merck), Par3 (rabbit polyclonal 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with an anti-puromycin antibody (mouse monoclonal 1:500, Merck) combined with an anti-β-actin antibody (rabbit polyclonal 1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... and phosphatase and proteinase inhibitor cocktails (P5726 and P2714; Sigma-Aldrich/Merck group, USA), and scrapped ...
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bioRxiv - Microbiology 2022Quote: ... the pellet was resuspended in 20 mL of Milli-Q water (Merck Group, Switzerland) and centrifuged again for 15 min at 3700 rpm ...
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bioRxiv - Plant Biology 2023Quote: ... Malathion (Malathion, PESTANAL®, analytical standard, Sigma-Aldrich, Merck Group, St Louis, MO, USA) was applied at 1000LgLha−1 of active ingredient ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Biophysics 2021Quote: ... The reconstituted receptors were cleared by moderate centrifugation via spin filter microtubes (Merck Millipore Ltd.) followed by size exclusion chromatography using a Superdex 200 Increase 10/300 GL column (GE ...
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bioRxiv - Molecular Biology 2022Quote: ... Glucocorticoid Receptor antibody (#3660, Cell Signalling Technology) or control IgG antibody (#03-198, Merck Millipore) and incubated for 1 h at room temperature whilst rotating ...
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bioRxiv - Physiology 2021Quote: ... and chemokine (C-X-C motif) ligand 1 (Cxcl1) were assessed using the MILLIPLEX-MAP kit Cytokine/Chemokine Magnetic Bead Panel (Merck Millipore, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Cell Biology 2022Quote: ... centrifuged at 3,000 × g at 4°C and filtered under sterile conditions using a 0.22 μm filter (Millex-GV, PVDF; Merck/Millipore Watford, Hertfordshire, UK), and is referred to as CM-OAT1.