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Citations for Merck :
101 - 150 of 4310 citations for Mouse Chemokine C X3 C Motif Receptor 1 CX3CR1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... were expressed as fusion proteins with 6x polyHis tail at the C-terminus in E.coli strain BL21(DE3) (Merck-Novagen, Darmstadt). After harvesting ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Cell Biology 2020Quote: ... as described above. The pretreatments with translational inhibitors puromycin (f. c. 20 µg/ml) and cycloheximide (f. c. 100 µg/ml) (Merck, Darmstadt, Germany) were performed after harvesting of the cells in the import buffer for 30 min at 37°C.
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bioRxiv - Plant Biology 2022Quote: ... The lysate was cleared by two centrifugations (4000 rpm, 10 min, 4 °C and 20000g, 10 min, 4°C) and filtered (Miracloth, Merck 475855-1R). The lysate (input ...
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bioRxiv - Cell Biology 2023Quote: ... The cell lysates were centrifuged at 16,000 x g for 10 minutes at 4°C and the supernatants were then incubated overnight at 4°C with anti-Flag M2 affinity gel beads (Merck, Cat.N. A2220). M2 beads were then pelleted and washed three times with lysis buffer containing protease inhibitors ...
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bioRxiv - Biochemistry 2023Quote: Total cell lysates of HEK293 cells stably expressing C-terminally Flag M2-tagged SIRT4 were incubated overnight at 4°C with anti-Flag M2 agarose beads (Merck, Darmstadt, Germany). The beads were washed three times with washing buffer (50 mM Tris-HCl ...
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bioRxiv - Cell Biology 2019Quote: ... rabbit α-Tenascin C (Merck Millipore Cat. No. AB19013) 1:150 ...
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bioRxiv - Microbiology 2021Quote: ... pET28a-c (cloning with NH2-terminal 6His tag, Merck), and pMal-c5x (cloning with the NH2-terminal maltose-binding protein (MBP ...
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bioRxiv - Neuroscience 2021Quote: ... 2.1 × 150 mm at 15°C (Merck, Darmstadt, Germany), and on a UPLC Waters Acquity (Waters Corp ...
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bioRxiv - Microbiology 2021Quote: ... Acetanilide (10.36 % N, 71.09 % C, Merck KGaA, Darmstadt, Germany) was used as the standard ...
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bioRxiv - Microbiology 2023Quote: ... Drops of antifoam C emulsion (Merck Life Science NV), diluted six times ...
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bioRxiv - Systems Biology 2023Quote: ... and 10 μL of IS C (Merck, Darmstadt, Germany) containing 0.40 nmol acylcoenzyme A (acyl-CoA ...
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bioRxiv - Microbiology 2020Quote: ... Samples were pre-treated with (1) 36 µl of Lysozyme [1% w/v in 1% PBS – 37°C/30min with intermittent shaking] (Merck KGaA, Germany) and then with (2 ...
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bioRxiv - Biophysics 2022Quote: HEK293T cells were cultured in 10 cm tissue culture treated dishes grown at 37 °C in a 5% CO2 atmosphere in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged (16,000 g, 10 min, 4°C) and supernatants measured for protein content using infrared spectroscopy (Merck Millipore, Darmstadt, Germany). Lysates were then reduced ...
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bioRxiv - Biochemistry 2023Quote: ... cells were harvested by centrifugation at 5,000 × g and 4°C for 5 min and lysed with BugBuster Protein Extraction Reagent (Merck Millipore, Burlington, MA) prepared in 20 mM sodium phosphate buffer (pH 7.4 ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue homogenisation and western blotting was performed with 50 μg of total protein as previously described.15 Membranes were incubated overnight with gentle agitation at 4 °C with the MSH3 antibody (Merck MSH3MAB(1F6)) in 5% milk PSB-T (phosphate buffer saline (PBS) ...
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bioRxiv - Neuroscience 2021Quote: ... then incubated overnight at 4°C with anti-KCC2 (1:200; Merck Life Sciences, Italy, #07-432) or anti-IGF-1R β (1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... samples were incubated overnight at 4°C with primary antibodies against puromycin (1:500, MABE343, Merck Millipore), βIII tubulin (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Cell Biology 2022Quote: ... DLD-1-WT mCherry-Sec61β and DLD-1-C-H3 mCherry-Sec61β stable cell lines were generated by GeneJuice (Merck Millipore) transfection of mCherry-Sec61β into the respective parental lines ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Pathology 2021Quote: ... 4°C and filtered with a 0.45μm filter (Merck, Germany). MLNs were mashed by sliding two glass slides together ...
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bioRxiv - Microbiology 2020Quote: ... c) Echinocandins: caspofungin (CPF, Merck & Co. Inc, Rahway, NJ, USA), micafungin (MCF ...
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bioRxiv - Neuroscience 2023Quote: Add anti-mitotic agent cytosine arabinoside (Ara-C, Merck - C6645) to top compartment ...
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bioRxiv - Cell Biology 2023Quote: ... and 2 µg of anti-c-myc (4A6; Merck Millipore). For immunoprecipitation of PER2::Luc constructs rabbit anti-firefly luciferase IgG (Abcam ...
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bioRxiv - Biophysics 2024Quote: ... cell were treated with 5 µg/mL Mitomycin C (Merck) for 1 hour ...
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bioRxiv - Neuroscience 2023Quote: ... The Luminex Milliplex® MAP human cytokine / chemokine magnetic bead panel (HSTCMAG28SK07; Merck) was used to detect cytokines ...
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bioRxiv - Microbiology 2024Quote: ... MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Customised 7-plex Panel (Merck Millipore) was used to quantify cytokine levels.
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bioRxiv - Biophysics 2019Quote: ... reduced and alkylated protein extracts were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, # MRCPRT010 Ultracel YM-10). Digested samples were desalted using ZipTips according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X-100) and 20μg was immunoprecipitated overnight at 4°C with 10μg S9.6 antibody (Merck, MABE1095) conjugated to 100μL Pierce ChIP-grade protein-A/G magnetic beads (Thermo ...
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bioRxiv - Microbiology 2022Quote: ... An aliquot of 5 μl was spotted on an activated (100 °C, 1 h) TLC sheet (Merck, USA). CM2 ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then incubated with primary antibody rabbit anti-c-fos (1:1000, Merck Millipore, Burlington, United States) in TBS (5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Choline acetyltransferase (ChAT) expression was detected with a goat polyclonal antibody (1:500; 4°C overnight, AB144p, Merck) and revealed with the secondary antibody Alexa Fluor 647 donkey anti-goat IgG (1:500 ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Immunology 2023Quote: Bovine cytokines and chemokines secreted in the supernatant by cells were measured using the commercial multiplex immunoassay MILLIPLEX MAP Bovine Cytokine/Chemokine Panel 1 (Merck/Sigma). Therefore ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were derivatized for 1 hour at 85 °C using N-tert-Butyldimethylsilyl-N-methyl-trifluroacetamide with 1% tert-Butyldimethylchlorosilane (Merck, NJ, USA) and immediately analysed by GC-MS ...
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bioRxiv - Cell Biology 2024Quote: ... Ovarioles were then incubated overnight at 4°C in PBT (0.2% Tween20) 1% BSA and a 1:200 of FITC-conjugated anti-α-tubulin antibody (F2168, Merck, clone DM1A). Ovarioles were then washed 6×10min in PBT (0.2% Tween20 ...
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bioRxiv - Bioengineering 2020Quote: ... Following PBS washes (5 min x3) and overnight incubation in blocking buffer (5% bovine serum albumin (Merck Millipore) in PBS ...
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bioRxiv - Biophysics 2019Quote: ... Constructs of Kmpv1 and KmpvSP1 were transiently expressed as fusion proteins with GFP on the C-terminus using the liposomal transfection reagent GeneJuice® (MERCK KGaA, Darmstadt, Germany). Measurements were performed at room temperature in a bath solution containing ...
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bioRxiv - Microbiology 2019Quote: ... was grown at 37°C in LYBHI medium (BHI medium (Merck) supplemented with 0.5% yeast extract (Difco) ...
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bioRxiv - Physiology 2020Quote: ... 3.) C + 0.3 g/kg vitamin E (Merck KGaA, Darmstadt, Germany) (vit E diet) ...
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bioRxiv - Microbiology 2023Quote: ... Isolates were grown overnight at 37°C on LB agar (Merck). Single colonies were inoculated into Mueller-Hinton broth (MHB ...
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bioRxiv - Microbiology 2023Quote: ... under microaerophilic conditions in anaerobic jars with Anaerocult C (Merck, USA) at 37°C for 72 h ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4 °C with a monoclonal anti-RBPMS antibody (1:500, Rabbit, ABN1362, Merck Millipore) for the retina ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4 °C with 100 µL of lysate diluted at 1:40 in carbonate buffer (Merck). After a blocking step with PBS with 4 % milk for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... and soluble protein was clarified by centrifugation (50,000×g, 1 h, 4°C) and subsequently passed through a 0.22-μm filter (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... at 37°C for 60 minutes followed by proteinase K solution treatment (7.5 μg ml−1 proteinase K; Merck, Darmstadt ...
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Immunology 2022Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), p-ERK (Cell Signaling Technology ...