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Citations for Merck :
701 - 750 of 985 citations for Mouse Alpha ketoglutarate dependent dioxygenase FTO FTO ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The Sigma Spectrum plant total RNA kit (Merck KGaA, Darmstadt, Germany) was used for RNA extraction that followed the manufacturer procedure ...
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bioRxiv - Genetics 2023Quote: ... using the Spectrum Plant Total RNA kit (Merck KGaA, Darmstadt, Germany). Gene expression was determined by RT-qPCR as previously described (Zenoni et al. ...
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bioRxiv - Physiology 2023Quote: ... glucose levels were determined using a Glucose (GO) assay kit (Merck). Blood insulin concentrations were measured by Rat Insulin ELISA kit (Mercodia) ...
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Critical contribution of mitochondria in the development of cardiomyopathy linked to desmin mutationbioRxiv - Pathology 2023Quote: ... Soluble proteins were then dosed using the Bicinchoninic Acid Kit (Merck) and diluted in Laemmli protein sample buffer ...
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bioRxiv - Cell Biology 2020Quote: ... GFP (1:2000) (Faix et al., 2001) or mouse monoclonal antibody against glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (1:1000; #CB1001-500UG, Merck (Darmstadt, Germany)) and anti-fascin antibody 5E2 (undiluted hybridoma supernatant) ...
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bioRxiv - Physiology 2021Quote: ... in maternal serum and placenta was performed with MILLIPLEX MAP Mouse Cytokine/Chemokine Magnetic Bead Panel – Immunology Multiplex Assays (MCYTOMAG-70K, Merck Millipore, Germany) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Neuroscience 2022Quote: ... for 5 min each at RT and incubated with secondary antibodies in TPBS-TS-1% for 4h at 4°C (Alexa Fluor 546 anti-Mouse (Merck, A-11018), Alexa Fluor 488 anti-goat (Abcam ...
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bioRxiv - Pathology 2022Quote: ... The sections were incubated overnight at 4°C in a pre-incubation solution containing mouse monoclonal em48 (Merck Millipore; MAB5374, 1:400) and rabbit polyclonal Laminin (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2022Quote: For immunocytochemical staining we used the following primary and secondary antibodies: mouse anti-NFL antibody clone DA2 (Merck Millipore, cat. no. MAB1615), mouse anti-NFL antibody clone F-12 (Santa Cruz Biotechnology ...
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bioRxiv - Plant Biology 2019Quote: ... membranes were immunoblotted with HRP-conjugated Flag antibodies (Monoclonal ANTI-FLAG® M2-Peroxidase HRP antibody produced in mouse, A5892; Merck-Millipore), HRP-conjugated HA antibodies (12013819001 ...
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Immunofluorescence staining of the DNA double-strand-break marker γH2AX [32] was performed using a JBW301 mouse monoclonal anti-γH2AX antibody (Merck, Darmstadt, Germany) and an Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Thermo Fisher ...
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bioRxiv - Neuroscience 2021Quote: ... and rabbit anti-Iba1 1:1000 (Wako Chemicals GmbH, Neuss) or double-stained with goat anti-td-Tomato and mouse anti-NeuN 1:500 (Merck Millipore, Darmstadt). Counter-staining for nuclei with 42,6-diamidin-2-phenylindol (DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... β-actin which was used as loading control was detected using a rabbit polyclonal IgG against mouse β-actin (A2103, Merck, Darmstadt, GER) diluted 1000-fold ...
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bioRxiv - Molecular Biology 2021Quote: ... The DNA fragment encoding mouse C/EBPβ residues 221–296 (bZIP domain) was cloned into pET-45b(+) vector (Novagen/Merck, Darmstadt, Germay) via ‘in vivo assembly’ (35 ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Cancer Biology 2022Quote: ... paraffin-embedded tissue sections were deparaffinized and rehydrated by standard methods. Thereafter, sections were incubated with monoclonal mouse anti-p53 (mutant, clone Ab-3 PAb240 catalogue no. OP29-200UG) (Merck, Darmstadt, Germany) diluted 1:100 in PBS at 4°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoreactive bands were detected with horseradish peroxidase-coupled anti-mouse or anti-rabbit antibodies (CST) using the ECL System (Merck Millipore; WBKLS0500). Immunoblot analysis was performed using the LI-COR Odyssey Imaging System.
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bioRxiv - Molecular Biology 2023Quote: ... The detection was performed using a specific secondary antibody coupled to the horseradish peroxidase (HRP): anti-mouse-HRP (1:4000, A4416 Merck, Sigma Aldrich), anti-rabbit-HRP (1:10000 ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
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bioRxiv - Plant Biology 2021Quote: ... the sections were incubated in 1× PBS containing 1% (1:100 dilution) mouse monoclonal anti-Flag M2 primary antibody (Merck, cat. no. F1804) and 0.1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 900 µl supernatant was transferred to a new tube and incubated with 25 µl (1/2 * OD in µl) of mouse-anti-FLAG antibody (clone M2, Merck/Sigma-Aldrich #F1804) at 4 °C for 45 min (rocking) ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Biophysics 2020Quote: ... Primary antibodies used to image centrioles in expanded RPE-1 cells were rabbit anti-polyglutamate chain (polyE), pAb (IN105, 1:250) and mouse monoclonal anti-acetylated tubulin (Merck, T7451, 1:500). Secondary antibodies were goat anti-Rabbit Alexa488 (Thermo-Fisher Scientific ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated (1 hour, 1/5000 dilution, RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the cells were stained with goat anti-rabbit Alexa Fluor® 555 (Thermo, 1:1,000) and mouse anti-IE Alexa Fluor® 488 (Merck, 1: 1,000) for 1 hour at RT ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then treated with antibodies against FLAG (Monoclonal ANTI-FLAG® M2 antibody produced in mouse; Merck CAT# F3165; 1:200 dilution) or CSNK1D (Anti-Casein Kinase 1 delta/CSNK1D antibody [AF12G4] ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2020Quote: ... ChIP assays were performed using a specific kit (Merck Millipore, Darmstadt, Germany). After crosslinking ...
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bioRxiv - Cell Biology 2020Quote: PP2A activity were measured by PP2A phosphatase activity assay kit from Merck Millipore (PP2A immunoprecipitation phosphatase assay kit ...
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bioRxiv - Microbiology 2021Quote: ... and a solution of XTT/PMS (Cell Proliferation Kit II; Merck, Germany) and culture medium was added to the wells ...
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bioRxiv - Cancer Biology 2020Quote: ... the ApopTag® Red In Situ Apoptosis Detection Kit (S7165, Merck Millipore) was also used to label apoptotic cells following Wells and Johnston protocol (Wells and Johnston ...
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bioRxiv - Cell Biology 2021Quote: RIP assays were performed using a Magna Nuclear RIP Kit from Merck Millipore (17-10520 ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
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bioRxiv - Molecular Biology 2022Quote: PLA detection was performed using a Duolink In Situ Orange Kit (Merck) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: We performed the proximity ligation assay129 using the Duolink PLA Kit (Merck) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... RNA was purified using the GenElute Mammalian total RNA Miniprep kit (MERCK). Samples from cell culture were harvested in the RNA extraction buffer supplemented with 10mM ß-ME ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cell viability was studied using Cell Counting Kit-8 (CCK8, Merck, Germany). VeroE6 cells were seeded at 10,000 cells per well of flat bottom 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The resulting RNA was then subjected to amplification (Merck WTA2 Amplification Kit) to produce cDNA that was subsequently purified by column capture (Qiagen QiaQuick PCR purification kit ...
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bioRxiv - Immunology 2021Quote: QCM Boyden chamber chemotaxis neutrophil migration assay kits were purchased from Merck Millipore (ECM505 ...
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bioRxiv - Microbiology 2021Quote: ... KOD Xtreme Hotstart DNA polymerase kit was obtained from Merck (Darmstadt, Germany). T4 DNA ligase was obtained from New England Biolabs (NEB) ...
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bioRxiv - Immunology 2022Quote: ... or with vaccine RNAs using RiboJuice™ mRNA Transfection Kit (Merck Millipore) according to the manufacturer’s instructions and incubated for 18 h ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the ApopTag Plus Fluorescein In Situ Apoptosis Detection Kit (Merck, Darmstadt, Germany) was used according to the manufacturer’s protocol ...