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Citations for Merck :
151 - 200 of 765 citations for MIF Macrophage migration inhibitory factor Mouse His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Biochemistry 2020Quote: ... the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Biophysics 2020Quote: ... the cells were incubated with a FCS-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days ...
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bioRxiv - Neuroscience 2021Quote: ... The cell medium was aspirated from the wells and fresh medium with one of the factors (Bafilomycin, 200 nM, Merck Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Once DRGs were removed and placed in ice-cold neuronal growth medium (Neurobasal medium, B27, and 10 ng/ml Nerve growth factor (NGF)-2.5 s (Merck Millipore), 2 mM L-glutamine ...
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bioRxiv - Cell Biology 2023Quote: HPAECs were seeded at the density of 10,000 cells/well in a pre-prepared 96-well plate containing 30μl of growth-factor reduced Matrigel (Merck, # CLS354230-1EA) per well (38) ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Molecular Biology 2021Quote: TEER measurements [22] were performed on 3rd day of incubation with growth factors and (or) different inhibitors by using Millicell ERS-2 Electrical Resistance System (Merck-Millipore). Each insert was measured in three different locations ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GAPDH (Merck), and rabbit anti-actin (Santa Cruz ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was sterile filtered (0.22 μm) and afterwards concentrated by the factor of 20 using 100 kDa molecular weight cut-off filters (Merck KGaA, Darmstadt, Germany). To determine the number of released vesicles ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Molecular Biology 2021Quote: ... or mouse IgG (NXA931, Merck) and 1mg/ml of heparin for at 4 ֯C for 16 hours with end-over-end rotation ...
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bioRxiv - Neuroscience 2020Quote: ... 3 (mouse monoclonal from Merck), GABA A Receptor γ (guinea pig polyclonal from SYSY) ...
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bioRxiv - Neuroscience 2021Quote: ... or mouse serum (Merck, M5905) as a mock condition (W ...
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bioRxiv - Systems Biology 2022Quote: ... mouse anti-GAPDH (CB1001, Merck), rabbit phospho-Rb Ser807/811 (8516 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-NeuN (Merck, MAB377), mouse anti-parvalbumin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-Rac1 (Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: ... BUBR1 (mouse mAb, Merck, MAB3612), CENP-A (mouse mAb ...
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bioRxiv - Cell Biology 2023Quote: ... tubulin (mouse mAb; Merck, T6199), BUB1 (rabbit pAb ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti-puromycin (Merck, MABE343) (1:10.000) ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-vinculin (V9131, Merck); rabbit anti-pYAP S127 (D9W21 ...
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bioRxiv - Cell Biology 2023Quote: ... mouse TAP952 (1:500, Merck). For testing the expression of TTLL5 ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-Vinculin (V9131, Merck), anti-Col1A1 (91144S ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-Vinculin (V9131, Merck); anti-Col1A1 (91144S ...
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bioRxiv - Immunology 2023Quote: ... and goat anti-mouse (Merck).
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bioRxiv - Neuroscience 2024Quote: ... or mouse anti-GAPDH (Merck) was diluted 1:1000 in blocking buffer ...
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bioRxiv - Genomics 2024Quote: ... Mouse anti-NeuN antibody (Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... EnSC were seeded at a clonal density of 30 cells/cm2 on 1 mg/ml fibronectin-coated 6-well plates and cultured in 10% DMEM/F12 medium supplemented with basic fibroblast growth factor (bFGF; 10 ng/ml; Merck Millipore, Watford, UK) for 10 days with a 50 % media change on day 7 ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-Flag M2 (F1804, Merck), rabbit anti-SOH (07-2139 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-Vinculin (#V9131-100UL, Merck), rabbit anti-TBK1 (ab40676 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-synaptopodin (Merck, 1:500), rabbit anti-calreticulin (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... Mouse anti-tau (1E1/A6, Merck) or rabbit anti-αS (ab138501 ...
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bioRxiv - Microbiology 2019Quote: ... mouse α-Lamin B1 (MAB5492, Merck), mouse α-PCNA (c907 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-TH (Merck, 1:1000), mouse anti-Bassoon (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-PSA-NCAM (Merck Millipore), rat anti-NCAM2 (R&D Systems) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-GAD67 (mouse monoclonal from Merck), anti-cleaved Caspase-3 (rabbit polyclonal from Cell Signaling Technology) ...
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bioRxiv - Cell Biology 2021Quote: ... Mouse monoclonal anti-Creb3L2 (Merck, MABE1018) and anti-GAPDH (Genetex ...