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Citations for Merck :
151 - 200 of 204 citations for Ion Exchange Chromatography since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... which were then extracted with pentane [18] for analysis by reversed-phase thin-layer chromatography (TLC) with HPTLC Silica gel 60 RP-18 (Merck) and an acetone/water (39:1 ...
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bioRxiv - Plant Biology 2021Quote: ... PIP was separated from the rest of the phospholipids by thin-layer chromatography (TLC) using K-oxalate-impregnated and heat-activated silica gel 60 plates (20×20 cm; Merck) and an alkaline TLC solvent ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further separated by size exclusion chromatography (HiLoad 26/600 Superdex 75, Cytiva) and concentrated using Ultra-4 or 15 mL centrifugal filter devices (Amicon, Merck). Correct size and high purity were verified via SDS-PAGE and LC-MS analysis ...
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bioRxiv - Plant Biology 2021Quote: ... Purity of compounds was monitored via thin layer chromatography (TLC) using pre-coated aluminium-backed plates (silica gel 60 F254, Merck) and compounds were visualised by UV radiation at 254 nm and then using an anisaldehyde spray reagent (1% p-anisaldehyde:2% H2SO4 ...
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bioRxiv - Microbiology 2020Quote: The qualitative analysis of hydrolyzed products was performed by thin-layer chromatography (TLC) on silica gel-coated aluminium foil (TLC Silica gel 60 F254 20×20 cm, Merck) for detecting sugars ...
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bioRxiv - Microbiology 2021Quote: ... Extracts were filled into a 100-μl Hamilton syringe and spotted onto silica gel 60 F254 high-performance thin-layer chromatography (HPTLC) plates (Merck) with a Linomat 5 sample application unit (Camag ...
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bioRxiv - Microbiology 2022Quote: ... 300 µg of lipids were separated by two-dimensional thin layer chromatography (TLC) onto glass-backed silica gel plates (Merck) 58 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Biochemistry 2020Quote: The reaction was monitored by thin-layer chromatography (TLC) performed on precoated Merck 60 GF254 silica gel plates (Merck, US) with a fluorescent indicator ...
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bioRxiv - Biochemistry 2021Quote: ... Chemical reactions toward the synthesis of tripeptidomimetic building block 11 were monitored via thin layer chromatography (TLC) using aluminum sheets with silica gel 60 F254 (Merck). Silica gel 0.060-0.200 mm ...
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bioRxiv - Biochemistry 2021Quote: ... Lipids containing [6-3H]GlcN-PI were dissolved in 100 μL of chloroform: methanol (2:1, by volume) and loaded to a high performance thin-layer chromatography (HPTLC) plate (Merck). The [6-3H]GlcN-PI was located by phosphorimaging ...
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bioRxiv - Cancer Biology 2021Quote: ... water for chromatography (LC-MS Grade) and acetonitrile hypergrade for LC-MS was from LiChrosolv® (Merck KGaA, Darmstadt, Germany).
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bioRxiv - Immunology 2020Quote: ... Samples were separated on a Thermo Scientific Accela liquid chromatography system coupled with a guard column (Hichrom) and a ZIC-pHILIC column (Merck). Separations were performed using a gradient elution in a solution of 20mM ammonium carbonate ...
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bioRxiv - Microbiology 2022Quote: ... appropriate amounts of polar lipid extracts were spotted onto silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG ...
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bioRxiv - Molecular Biology 2022Quote: ... Reaction progress was monitored with analytical thin-layer chromatography (TLC) on aluminum backed precoated silica gel 60 F254 plates (E. Merck). Column chromatography was carried out using silica gel 60 (230-400 mesh ...
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bioRxiv - Microbiology 2022Quote: ... and the chloroform or water phases were separated by one-dimensional thin layer chromatography (1D-TLC) on high-performance TLC aluminum sheets (silica gel 60; Merck). For separation of lipids ...
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bioRxiv - Cell Biology 2022Quote: ... cells were washed with 2 ml 4 °C PBS for 3 times and afterwards fixed in 1 ml of −20 °C cold methanol (Gradient grade for liquid chromatography, Merck) for 5 minutes at −20 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The purity of compounds was checked using 1H NMR or thin layer chromatography (TLC) using pre-coated aluminium-backed plates (silica gel 60 F254, Merck) and compounds were visualised by UV radiation at 254 nm and then using an anisaldehyde spray reagent (1% p-anisaldehyde:2% H2SO4 ...
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bioRxiv - Microbiology 2022Quote: ... Purification of compound 9 was carried out through column chromatography by passing the concentrated crude extract through a packed silica gel 60 (Merck) in a column ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were taken up in MeOH and spotted onto an activated thin layer chromatography (TLC) silica gel 60 plate (Merck). The plate was developed in CHCl3:MeOH:Acetic Acid:H20 (25:15:4:2 ...
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bioRxiv - Microbiology 2023Quote: ... The whole sample was applied to a high-performance thin-layer chromatography (HPTLC) silica gel 60 plate (10 x 10 cm; Merck) with a Hamilton syringe ...
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bioRxiv - Microbiology 2023Quote: ... Lipid extracts were then applied to silica gel high-performance thin-layer chromatography (HPTLC) plates (silica gel 60 F254, Merck) using a Linomat 5 sample application unit (CAMAG) ...
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bioRxiv - Bioengineering 2023Quote: ... The hydrolytic products were separated by thin layer chromatography (TLC) on Partisil K6 Silica gel 60 plates (250 µm thickness, 20 × 20 cm; Merck) with developing solvent ...
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bioRxiv - Cell Biology 2023Quote: ... APTS-labelled glycans were prepared for xCGE-LIF in a 1:10 dilution in water (water for chromatography, LC-MS grade, Merck) and mixed with 1 µl GeneScan™ 500 LIZ™ dye Size Standard (1:50 dilution in HiDi™ Formamide ...
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bioRxiv - Microbiology 2023Quote: Samples separation with chromatography was performed on the SeQuant ZIC-HILIC column (5 μm, 200 Å, 150x2.1 mm, Merck, Germany) with a flow rate of 0.6 mL/min ...
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bioRxiv - Biochemistry 2023Quote: ... Reactions were monitored by thin-layer chromatography on commercially available silica pre-coated aluminium-backed plates (Merck Kieselgel 60 F254). Visualisation was under UV light (254 nm and 366 nm) ...
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bioRxiv - Microbiology 2024Quote: ... Lipids were visualised with thin layer chromatography (TLC) by adding 20 μL of sample on a silica gel 60 F254 sheet (Merck) and placed in a chloroform/methanol/water (90:10:1 ...
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bioRxiv - Microbiology 2019Quote: ... We dissolved dried lipid extracts in 40 μL of 1:1 methanol:CHCl3 and used 5-10 μL of sample for thin layer chromatography (TLC) on TLC silica gel 60 plates (Merck, Germany) with a mixture of 65:25:10 CHCl3:CH3OH:CH3COOH for the mobile phase ...
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bioRxiv - Microbiology 2019Quote: ... TX 3211 Alpha Wipe, ITW Texwipe, Kernersville, US, 23×23 cm; 20 ml autoclaved ultra-pure water for chromatography, LiChrosolv, Merck Millipore). Three sampling sessions were performed ...
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bioRxiv - Biochemistry 2020Quote: ... Extracted lipids in the small intestine were separated on a high-performance thin-layer chromatography plate (HPTLC; silica gel 60 plates, Merck, Germany) [26] ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Plant Biology 2021Quote: ... lipid extracts were roughly separated into polar and non-polar lipids by thin-layer chromatography (TLC) using silica plates (TLC silica gel 60, Merck Millipore) developed with chloroform because the high amount of shikonin derivatives hampered the chemical analysis of TAG and polar lipids by LC-MS ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Microbiology 2022Quote: Thin-layer chromatography (TLC) was performed using silica gel 60 GF254 precoated aluminium backed plates (0.25 mm thickness; Merck, Darmstadt, Germany), and visualization was accomplished by irradiation with UV light at 254 nm ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... All reactions were monitored by LC/MS analysis or by thin-layer chromatography (TLC) using aluminium plates precoated with silica gel (silica gel 60 F254, Merck, US) impregnated with a fluorescent indicator ...
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bioRxiv - Microbiology 2023Quote: ... Thin layer chromatography was carried out using TLC Silica gel 60 F254 Glass plates 2.5 x 7.5 cm (Merck KGaA, Darmstadt, Germany) and visualized with a UV lamp (254/365nm UV/6-watt ...
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bioRxiv - Biochemistry 2020Quote: ... Two μM of the reaction mixture was spotted onto a thin layer chromatography (TLC) Silica Gel 60 F254 plate (Merck, Darmstadt, Germany). Subsequently ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were aliquoted and either lyophilized for size-exclusion chromatography (SEC) or desalted using ZipTip C18 pipette tips (Merck Millipore Ltd. Ireland) for LC-MS/MS analysis ...
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bioRxiv - Microbiology 2021Quote: Estrogen metabolites were separated on silica gel aluminum thin-layer chromatography (TLC) plates (Silica gel 60 F254: thickness, 0.2 mm; 20 × 20 cm; Merck, Darmstadt, Hesse, Germany). Dichloromethane:ethyl acetate:ethanol (7:2:0.025 ...
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bioRxiv - Neuroscience 2022Quote: ... Compounds 1-10 were purified by flash chromatography on high-purity grade silica gel (9385, 60 Å, 230-400 mesh) from Merck (Germany), producing hydrochlorides in the form of white powders.
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bioRxiv - Biochemistry 2020Quote: ... Tracer quality control and plasma metabolite analysis were performed with a LaChrom high-performance liquid chromatography (HPLC) system (Hitachi; Merck, Darmstadt, Germany) equipped with a Radiomatic 150TR flow-through radioisotope detector (Packard ...
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bioRxiv - Microbiology 2020Quote: ... The residue was then resuspended in 100 µl methanol and one µl of each sample analysed by thin layer chromatography (TLC) using silica gel 60 F254 (Merck Millipore, Germany) with chloroform-acetic acid-ethanol (90:5:10 [vol/vol] ...
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bioRxiv - Microbiology 2021Quote: ... Reactions were monitored by thin layer chromatography (TLC) using TLC plates pre-coated with silica gel 60 F254 (Merck KGaA, Darmstadt, Germany), visualized with a handheld ultraviolet device either directly or after staining with 5% H2SO4 in ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... The total extract of proteins was then centrifuged for 20 min at 20,000 g and the soluble fraction loaded on an affinity chromatography with 2 ml of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). The resin was washed with 20 ml of 20 mmol/L Tris-HCl (pH7.9 ...
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bioRxiv - Microbiology 2022Quote: ... butyrate and propionate) were quantified in colonic samples from luminal phase by high-performance liquid chromatography (HPLC) (Elite LaChrom, Merck Hitachi, USA) coupled with a DAD diode as described in (28).
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bioRxiv - Cancer Biology 2023Quote: A Thermo Ultimate 3000 high performance liquid chromatography (HPLC) system was equipped with a ZIC-pHILIC column (SeQuant; 150 mm by 2.1 mm, 5 μm; Merck KGaA, Darmstadt, Germany), with a ZIC-pHILIC guard column (SeQuant ...
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bioRxiv - Synthetic Biology 2023Quote: ... All moisture-sensitive reactions were carried out in oven-dried glassware under N2 Thin-layer chromatograms (TLC) and flash chromatography separations were respectively performed on precoated silica gel 60 F254 plates (Merck, 0.25 mm) and on silica gel 60 (230-400 mesh) ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µL (= 5 mg fresh weight) of total lipids were applied onto a silica-coated chromatography plate (Silicagel 60 F254, 10x 20 cm; Merck, Rahway, NJ) and developed with hexane/ethyl ether/acetic acid (21 ...
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bioRxiv - Biochemistry 2020Quote: ... Total extract was clarified by 20 min centrifugation at 30,000 rcf at 4°C and the soluble fraction was loaded on affinity chromatography over 5 mL of NiNTA resin (Sigma-Aldrich Merck, Darmstad Germany). Resin was step-washed with 10 mL of buffer A supplemented with 10 ...