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Citations for Merck :
251 - 296 of 296 citations for Insulysin IDE Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: Isolation of fresh human PBMCs was initiated within 1 h after blood collection using Histopaque® 1077 (10771; Merck KGaA) and standard density centrifugation (800 rcf ...
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bioRxiv - Bioengineering 2021Quote: The rat INS-1 832/3 cell line (insulinoma cell line stably transfected with human insulin; hereinafter INS-1) was obtained from Merck. A HUVEC (human umbilical vein endothelial cell ...
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bioRxiv - Cancer Biology 2021Quote: ... and SPPL2A and control siRNA (IDT) were transfected into two human cell lines (HMV-2/CVCL_1282 -Merck-, WM3211/CVCL_6797 -Rockland-) using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Genetics 2021Quote: ... At day 10 cells were passaged at a 2:3 ratio into 12 well cell culture plates coated with 15 µg/ml human plasma fibronectin (Merck) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... Aliquots of our working standard were generated by spiking 50 μL of our generated VLPs into 950 μL of human plasma (Merck). The calibration curve samples and the aliquots of working standard were then tested using a cobas® 6800 system (Roche ...
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bioRxiv - Developmental Biology 2021Quote: ... 500 μg/mL rice-derived recombinant human albumin and 213 μg/mL L-ascorbic acid 2-phosphate (both from Merck). After 24 h of CHIR99021 stimulation ...
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Molecular Biology 2020Quote: ... Another washing step was conducted as before and the Anti-Human IgE (ε-chain specific) detecting antibody (Merck, Darmstadt, Germany) was diluted 1:1000 (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Biochemistry 2023Quote: DNA encoding the TBD domain of human ASK1 (residues 88–267) was ligated into pRSFDuet-1 (Merck KGaA, Darmstadt, Germany) using BamHI and NotI sites ...
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bioRxiv - Biophysics 2023Quote: ... for 15 min at 20°C and the stamps were dried under a nitrogen flow A solution was then prepared by mixing 40 µL human fibronectin (Merck) and 960 µL of demineralized water ...
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bioRxiv - Developmental Biology 2023Quote: Ovulation was induced by injecting adult female Xenopus laevis with 600 units of human chorionic gonadotropin (HCG, MERCK Animal Health) and animals were kept at 16 dc overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... T cell medium (human serum was replaced with FCS during thawing) and incubated for 15 minutes with 25U/mL benzonase (Merck). After washing ...
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bioRxiv - Biochemistry 2020Quote: ... cells were stimulated for the indicated periods of time with antibodies to human CD3 (7.5 μg/mL, Cat. number 217570, Merck; Jurkat cells) and EGF (50 ng/mL ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... PAb directed against phospho-STAT2 (Tyr689) and MAb directed against human IFNα/β receptor chain 2 (MAB1155) were purchased from Merck-Millipore.
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bioRxiv - Biochemistry 2021Quote: ... These Wild Type or Mutant Type let-7a-5p plasmids were co□transfected into treated human chondrocytes cells along with NC mimics or KCNQ1OT1 mimic (Sigma□Aldrich; Merck KGaA) using Lipofectamine 6000 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... and the Human Cytokine/Chemokine Magnetic Bead Panel (Cat# HCYTMAG-60K-PX30) on a Luminex MAGPIX system (Merck Millipore, Billerica, MA). Conditioned media concentrations (pg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Aliquots of them were analyzed with the MILLIPLEX human cytokine/chemokine magnetic bead panel (HCYTOMAG-60K, Merck Millipore, Burlington, MA, USA) using the Luminex 200 multiplexing instruments (40-012 ...
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bioRxiv - Molecular Biology 2020Quote: ... mt1 and mt2 were generated by inserting the ORF of human TruB1 and its mutants into the pET22b vector (Merck Millipore) at the NdeI and XhoI sites ...
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bioRxiv - Neuroscience 2021Quote: ... pSIH-mCherry-KDEL construct expressing lentiviruses were added to the suspension of motoneurons before plating on polyornithine-(PORN) and human merosin-coated (CC085, Merck-Millipore) plates ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Neuroscience 2022Quote: Human Neural Stem Cells (hNSCs) derived from the NIH approved H9 (WA09) human embryonic stem cell line were purchased from Merck (Germany). The hNSCs were plated on matrigel-coated 100 mm petri dishes and maintained in culture with NSCs expansion media (KO DMEM/F12 ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2023Quote: ... or human post-mortem total protein/ synaptoneurosome samples were then mixed into equal volumes of 2X Laemmli buffer (Merck: S3401- 10VL) and boiled for 10 minutes at 98 °C ...
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bioRxiv - Immunology 2023Quote: ... and PBMCs (1 × 106 cells per well) were seeded in 96-well plates in RPMI supplemented with 1% PNS and 10% AB human serum (Merck, UK) and stimulated with SARS-CoV-2 specific peptides pools ...
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bioRxiv - Developmental Biology 2023Quote: ... healthy female cynomolgus monkeys were subjected to follicular stimulation by intramuscular injection of 20 IU of recombinant human follitropin alpha (rhFSH, Gonal F, Merck Serono) for 8 days ...
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bioRxiv - Developmental Biology 2023Quote: ... a micro-infusion pump (iPRECIO SMP-200, ALZET Osmotic Pumps) with 15 IU/kg human follicle-stimulating hormone (hFSH, Gonal-f; Merck Biopharma) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Immunology 2024Quote: ... Primary human T-cells were isolated from peripheral blood (NHS Blood & Transplant Service, UK) using Ficoll-PaqueTM (Merck Group, Darmstadt, Germany) density gradient centrifugation followed by positive selection using antibody-coated magnetic beads directed against either human CD4 or CD8 antigen (Miltenyi Biotechnology ...
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bioRxiv - Neuroscience 2024Quote: ... MACS-enriched from fresh PBMC were seeded at 0.5×106 cells/cm2 in RPMI plus 5% heat-inactivated human AB serum (Merck Life Sciences) and 100 U/ml penicillin-streptomycin and cultured with 50 ng/ml rhIL-4 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: CM was collected from TIS MDA-MB-231 cells for chemokine and cytokine analyses using a Milliplex MAP human cytokine/chemokine magnetic bead panel (Merck Millipore) according to the manufacturer’s protocol.
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bioRxiv - Developmental Biology 2021Quote: ... males were boosted with 100 units (U) of human chorionic gonadotropin (HCG: Chorulon®, Merck, NADA NO.140-927, Code No. 133754) and females were primed with 10U HCG ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... rat slices were exposed to 3 μM dihydroethidium (DHE) while in humans’ sections it was added 4 μM DHE (Merck, Darmstadt, Germany) for 30 minutes at 37ºC 51 ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2020Quote: Eggs were collected from female Xenopus laevis frogs 16-18 hours after egg laying was induced by injection of 500 U of human chorionic gonadotropin (Chorulon from Merck, Sigma #CG10-10VL). Cytostatic factor-(CSF- ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Neuroscience 2021Quote: ... This iPS cell line was obtained by reprogrammation of a commercial fibroblast cell line (FibroGRO™ Xeno-Free Human Foreskin Fibroblasts, #SCC058, Merck Millipore, Germany). The patient hiPSC cell line was obtained by reprogrammation of skin primary fibroblasts from the female patient II.2 ...
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DJ-1 (Park7) affects the gut microbiome, metabolites and development of Innate Lymphoid cells (ILCs)bioRxiv - Immunology 2019Quote: ... Membranes were then washed twice 5 min in TBST and incubated with the primary antibody over night at 4°C (human and mouse a-syn: 610786 BD Biosciences; GAPDH: #5174, Cell Signaling and GFAP #MAB360, Merck Millipore, Germany). After incubation with the first antibody ...
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bioRxiv - Pathology 2021Quote: ... Cytokine and chemokine levels in monkey sera were measured using a MILLIPLEX MAP Non-Human Primate Cytokine Magnetic Bead Panel - Premixed 23 Plex - Immunology (Milliplex MAP kit, Merck Millipore, Burlington, MA), which includes the following 23 cytokines and chemokines ...
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bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Developmental Biology 2023Quote: ... Leuplin for Injection Kit), a micro-infusion pump (ALZET Osmotic Pumps, iPRECIO SMP-200) with 15 IU/kg human FSH (hFSH, Merck Biopharma, Gonal-f) was embedded subcutaneously under anaesthesia and injected 7 µL/h for 10 days ...
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bioRxiv - Genetics 2024Quote: ... This iPS cell line was obtained by reprogramming of a commercial fibroblast cell line (FibroGRO™ Xeno-Free Human Foreskin Fibroblasts, #SCC058, Merck Millipore, Germany). Patients’ hiPSC cell lines were obtained by reprogramming of skin primary fibroblasts from ...
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bioRxiv - Cell Biology 2019Quote: ... and subsequently with 5IU of human chorionic gonadotropin (hCG) (Intervet, GesmbH) were mated and zygotes were isolated in M2 media (Merck Millipore, MR-015P-D) and cultured in KSOM medium (Cosmo Bio Co. ...
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bioRxiv - Immunology 2023Quote: Concentrations of cytokines in supernatants of T cell cultures were assessed with the MILLIPLEX MAP Human Th17 magnetic bead panel kit (Merck Millipore, Burlington, MA, USA) and the Luminex® 200™ system according to the guidelines of the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: We used human APOL1 gene locus transgenic mice (BAC/APOL1 mice)17,45 with APOL1-G0 and APOL1-G1 heterozygotes (Merck & Co., Inc. (Rahway, NJ, USA)) ...
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bioRxiv - Bioengineering 2021Quote: Culture of human BM-MSCs and NE-MSCs with biomaterials performed in Corning® Costar® ultra-low attachment 24 well plates (Merck KGaA, Darmstadt, Germany) with Gibco™ α-MEM (Thermo Fisher) ...