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Citations for Merck :
51 - 100 of 672 citations for IMPA3 Mouse HEK 293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Cell Biology 2019Quote: ... GAPDH (mouse; Merck), FLAG (mouse ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG (mouse; Merck), FLAG (rat ...
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GAPDH (Merck), and rabbit anti-actin (Santa Cruz ...
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bioRxiv - Immunology 2023Quote: ... Lysate was clarified by spinning cells for 10 min at 20,000 × g before loading supernatant onto cOmplete™ His-Tag purification resin (Merck; cat # 5893682001). Bound proteins were washed with 20 mM tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Molecular Biology 2021Quote: ... or mouse IgG (NXA931, Merck) and 1mg/ml of heparin for at 4 ֯C for 16 hours with end-over-end rotation ...
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bioRxiv - Neuroscience 2020Quote: ... 3 (mouse monoclonal from Merck), GABA A Receptor γ (guinea pig polyclonal from SYSY) ...
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bioRxiv - Neuroscience 2021Quote: ... or mouse serum (Merck, M5905) as a mock condition (W ...
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bioRxiv - Systems Biology 2022Quote: ... mouse anti-GAPDH (CB1001, Merck), rabbit phospho-Rb Ser807/811 (8516 ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-NeuN (Merck, MAB377), mouse anti-parvalbumin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... mouse monoclonal anti-Rac1 (Merck Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-vinculin (V9131, Merck); rabbit anti-pYAP S127 (D9W21 ...
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bioRxiv - Cell Biology 2023Quote: ... BUBR1 (mouse mAb, Merck, MAB3612), CENP-A (mouse mAb ...
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bioRxiv - Cell Biology 2023Quote: ... tubulin (mouse mAb; Merck, T6199), BUB1 (rabbit pAb ...
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bioRxiv - Microbiology 2022Quote: ... mouse anti-puromycin (Merck, MABE343) (1:10.000) ...
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bioRxiv - Cell Biology 2023Quote: ... mouse TAP952 (1:500, Merck). For testing the expression of TTLL5 ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-Vinculin (V9131, Merck), anti-Col1A1 (91144S ...
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bioRxiv - Bioengineering 2023Quote: ... mouse anti-Vinculin (V9131, Merck); anti-Col1A1 (91144S ...
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bioRxiv - Immunology 2023Quote: ... and goat anti-mouse (Merck).
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bioRxiv - Genomics 2024Quote: ... Mouse anti-NeuN antibody (Merck Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... or mouse anti-GAPDH (Merck) was diluted 1:1000 in blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-Flag M2 (F1804, Merck), rabbit anti-SOH (07-2139 ...
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bioRxiv - Cell Biology 2022Quote: ... mouse anti-Vinculin (#V9131-100UL, Merck), rabbit anti-TBK1 (ab40676 ...
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bioRxiv - Neuroscience 2019Quote: ... mouse anti-synaptopodin (Merck, 1:500), rabbit anti-calreticulin (Abcam ...
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bioRxiv - Biochemistry 2019Quote: ... Mouse anti-tau (1E1/A6, Merck) or rabbit anti-αS (ab138501 ...
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bioRxiv - Microbiology 2019Quote: ... mouse α-Lamin B1 (MAB5492, Merck), mouse α-PCNA (c907 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-TH (Merck, 1:1000), mouse anti-Bassoon (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-PSA-NCAM (Merck Millipore), rat anti-NCAM2 (R&D Systems) ...
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bioRxiv - Neuroscience 2020Quote: ... anti-GAD67 (mouse monoclonal from Merck), anti-cleaved Caspase-3 (rabbit polyclonal from Cell Signaling Technology) ...