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Citations for Merck :
1 - 50 of 333 citations for IL 13 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... was centrifuged at 1500 rpm for 10 mins and the supernatant was used to measure inflammatory cytokines (IL-4, IL-5, IL-13, γ-INF) by LUMINEX multi-factor detection (MERCK). The cell pellets were fixed in 4% formaldehyde for Wright-Giemsa staining and total cells were counted and classified in each slide.
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bioRxiv - Cell Biology 2024Quote: Human embryonic kidney (HEK293) cells were cultured in Dulbeccoʹs Modified Eagleʹs Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Genomics 2020Quote: ... was incubated with/without 0.4μg human 6X-His-CDK9/Cyclin T1 (Merck, 140685) and/or 0.4μg human GST-tagged PP2A-α (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Growth medium was then removed from apical side and on the basolateral side it was replaced by ALI differentiation medium +/- 10 ng/ml IL-13 (IL012; Merck Millipore). Once the ALI interface was established medium was exchanged every second day till day 25-28 on ALI ...
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bioRxiv - Microbiology 2020Quote: ... the apical medium was removed and basolateral medium was switched to differentiation medium ± 10 ng/ml IL-13 (IL012; Merck Millipore). Differentiation medium consisted of a 50:50 mixture of DMEM-H and LHC Basal (Thermo Fisher ...
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bioRxiv - Systems Biology 2020Quote: ... biotin (13 μM, Merck, B4639) was added for protein biotinylation ...
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bioRxiv - Molecular Biology 2022Quote: ... or anti-His (Merck) for His-G5845–1508) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: For Phorbol 12-myristate 13-acetate (PMA, Merck) treatment ...
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bioRxiv - Plant Biology 2020Quote: ... anti-phospho-MBP (13-104, 1:200, Merck) or phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2024Quote: ... INS-832/13 cell line (SCC207, Merck Millipore) was cultured in RPMI 1640 medium supplemented with 10% FBS ...
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bioRxiv - Synthetic Biology 2021Quote: HEK293 and NIH-3T3 cells were cultured in DMEM (Merck) supplemented with 10% FBS (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 (Merck), JQ-1 (Sigma Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... His-tagged proteins were immobilized on Ni-NTA His-Bind® Superflow™ Resin (Merck Millipore, 70691), washed and eluted in SB buffer (30 mM HEPES-NaOH pH 7.5 ...
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bioRxiv - Genomics 2022Quote: ... 13 mm diameter filter membrane (GVWP01300, Merck Sigma Aldrich) using a 13 mm diameter stainless steel Swinny filter holder (XX3001200 ...
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bioRxiv - Genetics 2023Quote: Phorbol 12-myristate 13-acetate (PMA; Merck, Darmstadt, Germany) was dissolved in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg dephosphorylated myelin basic protein (Merck, 13-110), and purified MAK protein ...
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bioRxiv - Cancer Biology 2022Quote: ... whereas HEK293 cells were cultured in DMEM (D5796 MERCK, Darmstadt, Germany) containing 10% (vol./vol. ...
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bioRxiv - Molecular Biology 2023Quote: ... HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Merck) supplemented with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2023Quote: ... supplemented with 10% HI-FBS (Merck/Biochrom), 2 mM L-glutamine (Merck/Biochrom) ...
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bioRxiv - Systems Biology 2023Quote: ... Primary his tag antibody (05-949, Merck), stored in a skim milk solution ...
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bioRxiv - Molecular Biology 2024Quote: ... or pET30c (HIS) (Merck/Novagen, Darmstadt, Germany) using EcoRI and NotI sites ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were incubated with 50 µM 17ODYA (17-octadecynoic acid, Merck) added from stock solution in DMSO or with 0.05% DMSO in DMEM containing 2% charcoal-stripped FBS and 30 mM HEPES for 4 h at 37°C ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: HEK293 cells were cultured in Dulbecco⍰s Modified Eagle⍰s Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2019Quote: ... Dissected stage 13 anthers were also stained with iodine solution (Merck) to reveal pollen development and anther dehiscence.
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... concentrated to less than 13 mL using Amicon Ultra 10 K (Merck), and subjected to gel filtration chromatography on a HiLoad 26/600 Superdex 200 pg ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Neuroscience 2024Quote: ... we used fixed HEK293 cells labeled with primary antibody (rabbit anti-GM130, 1:1000, Merck) and secondary antibody (goat anti-rabbit conjugated with Alexa Fluor 555 ...
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bioRxiv - Immunology 2022Quote: ... IL-6 and IL-1RA was performed using the Luminex color-coded antibody-immobilized beads from Merck Millipore following the manufacture’s indications ...
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bioRxiv - Systems Biology 2020Quote: ... both parental and stable cells were supplemented with biotin (13 μM, Merck, B4639) for protein biotinylation ...
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bioRxiv - Immunology 2022Quote: ... and 10 μg/mL phorbol 12-myristate 13-acetate (PMA) and Ionomycin (Merck) to corresponding positive control wells ...
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bioRxiv - Cell Biology 2022Quote: ... and Myelin basic protien (MBP) as substrates (MBP, Dephosphorylated, Cat# 13-110, Merck). For each reaction ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... previously coupled to 1 ml HI-TRAP NHS columns (GE/Merck) packed with Sepharose pre-activated for covalent coupling of ligands containing primary amino groups ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Microbiology 2020Quote: ... 13 mM glycine and 3 μM thiamine (all compounds from Merck Millipore, Darmstadt, Germany).
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bioRxiv - Genomics 2022Quote: ... using a 13 mm diameter stainless steel Swinny filter holder (XX3001200, Merck Sigma Aldrich). We discarded the flowthrough of the PVDF membrane and transferred the membrane immediately after filtration to a 0.7 mm Garnet particle containing tube (13123-50 ...
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bioRxiv - Microbiology 2023Quote: ... Macrophages were differentiated with 200 nM phorbol 12-myristate 13-acetate (PMA) (Merck KGaA) following a previously described method with slight modifications [52] ...
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bioRxiv - Bioengineering 2024Quote: ... Unlabeled AAVs were detected on HEK293 cells post fixation by anti-AAV9 clone HL2372 (Merck, MABF2309-100UL) at a 1:500 dilution followed by AF647-labeled anti mIgG H+L.
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bioRxiv - Immunology 2021Quote: ... Cells stimulated with a cocktail of PMA (phorbol 12-myristate 13-acetate) (20 ng; Merck), ionomycin (500 ng ...
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bioRxiv - Molecular Biology 2024Quote: ... monocytes were seeded with 100 nM of phorbol 12-myristate 13-acetate (Sigma-Aldrich, Merck) into a 96-well tissue culture plate (60,000 cells/well ...
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bioRxiv - Microbiology 2021Quote: ... and the supernatant was incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was loaded onto a His-bind resin column (Merck Millipore) (1 ml ...
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bioRxiv - Plant Biology 2023Quote: ... and purified with Ni-NTA His•Bind® Resin agarose (Merck, 70666). The binding reaction was performed in 20 µL of binding buffer [10 mM Tris (pH 8.0) ...
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bioRxiv - Biochemistry 2023Quote: ... His tagged proteins were labeled using Ni-NTA-ATTO 488 (Merck Group) at a ratio of ATTO/protein = 1/500 in MN buffer (50 mM MES pH 6 ...
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bioRxiv - Biochemistry 2019Quote: ... 5 was performed using 600 μL HEK293 cells (±CL) previously treated with 2000 U/mL Benzonase (Merck, 70664) in the recommended buffer (50 mM Tris ...