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Citations for Merck :
301 - 350 of 4175 citations for Huntingtin Interacting Protein 1 Related Protein HIP1R Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... lysates were incubated with anti-HA agarose beads (20 μl of beads per 1 mg of proteins) (Merck, Darmstadt, Germany) for 2 h ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Microbiology 2023Quote: ... binding of equine antibodies was detected by incubation for 1 hour with protein A conjugated to peroxidase (GE) and visualized with chemiluminescence reagent (ECL, Merck). Images were obtained with an Odyssey LI-COR instrument ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Biochemistry 2020Quote: ... epithelium suspension cell line expressing the EBNA-1 protein (female origin) was cultured in EX-CELL(r) 293 Serum-Free Medium (Merck, 14571C) containing 4 mM GlutaMAX supplement (TermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... The supernatant fraction was collected and incubated with S-protein beads (Merck) for 2 hours ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with 50 kDa cut off ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein was concentrated using an Amicon 10 kD filter column (Merck UFC801008D) and purity tested by SDS-PAGE.
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bioRxiv - Molecular Biology 2021Quote: ... proteins were transferred from the gel to a PVDF membrane (Merck, IPFL00010) by semi-dry transfer with Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... Protein concentrations were measured using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Physiology 2020Quote: ... proteins were transferred to a PVDF membrane (Immobilon-P, Merck Millipore, IPVH00010) with 160 mA for 90 min ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were visualized by ECL (Enhanced chemiluminescence; Merck Millipore, Cat. No. WBKLS0500). The signal was detected using Vilber FUSION-SL system ...
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bioRxiv - Neuroscience 2020Quote: ... the protein solution was filtered through a 100 kDa filter (MERCK, MRCFOR100). The filtrate was transferred to a low-protein binding tube and 20 equivalents of dopamine (final concentration ...
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bioRxiv - Biochemistry 2021Quote: ... All remaining protein constructs were cloned into the pET28b vector (Merck Millipore). Unless otherwise stated ...
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bioRxiv - Biophysics 2021Quote: ... Separated proteins were transferred onto a PVDF membrane (IPVH00010, Immobilon-P; Merck) and incubated for 2hr with blocking buffer containing 5% Skimmed milk (70166 ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were measured using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Microbiology 2020Quote: Infected insect cells were disrupted with CytoBuster™ protein extraction reagent (Merck) and clarified by centrifugation at 4,300 × g for 20m before column loading ...
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bioRxiv - Microbiology 2020Quote: ... Target proteins were visualized with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Microbiology 2019Quote: ... Protein quantification was performed using the Direct Detect® system (Merck-Millipore). Each sample was set to 40 µg of total protein in 100 µl in 100 mM TEAB ...
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bioRxiv - Microbiology 2020Quote: ... GST bound protein was then cleaved using biotinylated thrombin (Merck Millipore; 69672) according to the manufacturers instructions overnight at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... proteins were transferred onto the methanol-activated µm PVDF membrane (Merck #ISEQ85R) using 1X transfer buffer (2.5 mM Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2021Quote: The Magna RNA-binding protein immunoprecipitation kit (Merck Millipore, Billerica, MA, USA) was applied for the assay ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The protein was filtered through a 0.22 μm Millex-GV filter (Merck) to remove potential aggregates ...
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bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were concentrated using 30K Amicon Ultra Centrifugal Filters (Merck, Darmstadt, Germany), flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Merck Millipore, MA, U.S.A). The membranes were incubated for 1h using 5% low-fat milk solution in 1X TBS (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were electrotransferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Merck) at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Following transfer of the proteins onto a PVDF membrane (Merck Milipore, ISEQ00010), the blots were saturated with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore), which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404 ...
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bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resolved proteins were electrotransferred to an Immobilon-P membrane (Merck, IPVH00010). The membrane was probed with specific antibodies listed in Supplementary Table 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... The desalted protein was concentrated on an Amicon 10,000 MWCO (Merck, Germany) and filtered through a 0.22 µM membrane.
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bioRxiv - Microbiology 2019Quote: ... Total protein was isolated from whole cell lysates using BugBuster® (Merck). Total protein concentrations were quantified by Pierce BCA quantification kit (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2019Quote: ... Fractions with addition of thrombin protease (5 Units/mg protein, Merck, www.merckmillipore.com) were pooled and dialyzed against dialysis buffer (10 mM Tris ...
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bioRxiv - Cancer Biology 2019Quote: ... and resolved protein was transferred to Immobilon-P PVDF 0.45µm Membrane (Merck). Proteins were detected using primary antibodies to Actin (Santa Cruz ...
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bioRxiv - Molecular Biology 2021Quote: ... after which the proteins were transferred to Immobilon P membranes (Merck Millipore). After blocking the membrane with 5% skimmed milk in TBS-T buffer solution for 1 h at RT or overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... protein was concentrated using a centrifugation filter (3K, Amicon, Merck, Darmstadt, Germany) up to a final concentration of 5 mg/mL and stored at −80°C.
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bioRxiv - Biophysics 2019Quote: ... Protein complex was concentrated with Amicon® Ultra Centrifugal Filters (Merck Millipore) and further purified on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with respective molecular weight cut offs ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore cat. No. IPFL00010) after which the membrane was blocked using Odyssey Blocking buffer (PBS ...
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bioRxiv - Biophysics 2021Quote: ... proteins were separated from salts and concentrated using Amicon Ultra 15 (Merck). Full length DnaK chaperone was purified by Ni2+-NTA column ...
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bioRxiv - Neuroscience 2020Quote: ... Thirty μl of PureProteome™ Protein A/G Mix Magnetic Beads (Merck) was washed in TBST and then incubated with 5 μl anti-HA antibody for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... mutant proteins to the cyanogen bromide (CNBr) activated sepharose 4b resin (Merck). Resin was activated by swelling it into 1 mM HCl according to the manufacturer’s instructions and equilibrated with a coupling buffer (20 mM sodium phosphate pH 8 ...