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Citations for Merck :
201 - 250 of 3247 citations for Human V Set And Immunoglobulin Domain Containing Protein 2 VSIG2 CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... 500 μg protein was added to spin columns (Pierce) containing anti-FLAG M2 affinity resin (Merck) and allowed to incubate rotating for two hours at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Biochemistry 2023Quote: ... Fractions containing full-size protein were concentrated using Amicon®Ultra 4 mL centrifugal filters (Merck). After the addition of 10% glycerol ...
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Cell Biology 2023Quote: Caco-2 cells were co-transfected with eSpCas9-GFP protein (Merck, cat #ECAS9GFPPR) and sygRNA (Merck ...
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bioRxiv - Biophysics 2021Quote: ... This mix included 98% unlabeled recombinant human E254N tubulin diluted in assay buffer containing oxygen scavengers (180 mg/ml catalase (C40, Merck) and 752 mg/ml glucose oxidase (22778.01 ...
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bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 10 % (v/v) glycerol, 1% (w/v) digitonin (Carl Roth GmbH, Karlsruhe, Germany) and cOmplete protease inhibitor (Merck). Samples were rotated on a wheel for 1h ...
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bioRxiv - Biophysics 2021Quote: ... and 10% (v/v) fetal bovine serum (Merck Millipore, Burlington, MA, USA). U-2 OS cells (ATCC ...
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bioRxiv - Biochemistry 2022Quote: ... 10% (v/v) glycerol) by using Amicon 10K Concentrator columns (Merck Millipore). SDS-polyacrylamid gel electrophoresis and spectrophotometric analysis was used to check purity and approximate quantity of proteins.
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 10% (v/v) fetal calf serum (Merck Biochrom, Berlin, Germany) and 1% (v/v ...
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bioRxiv - Biophysics 2022Quote: ... Three 5-minute washes in 0.1 v/v% Tween-20 (Merck KGaA) in PBS was included after both primary and secondary antibody staining.
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with 0.05% (v/v) proteinase K (Roche, Merck, Darmstadt, Germany) dissolved in 0.1 M Tris-HCl (pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: Proteins were extracted from 50 hpf zebrafish embryos using the lysis buffer from the calpain 1/2 activity kit (InnoZyme™ Calpain 1/2 Activity Assay Kit CBA054 purchased from Merck Millipore). Protein concentration was adjusted to 2 mg/mL and calpain enzymatic activity was measured in microtubes following the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... C3 and C1 were analyzed with HCMP2MAG-19K-02 Human Complement Magnetic Bead Panel 2 (Merck Millipore). Supernatants from non-stimulated CTL and L2-PD astrocytes cultured for 14 days were collected and stored at −80°C for long storage ...
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bioRxiv - Neuroscience 2019Quote: Plasma levels of adiponectin and leptin were measured using commercially available kits (Human Leptin Enzyme Immunoassay, Merck, Cat. #A05174 and Human Adiponectin ELISA, Merck, Cat. # EZHADP-61K). The minimum detectable dose of leptin was 7.8 pg/mL and that of adiponectin was 0.891 μg/mL.
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... media was replaced with media containing 10 nM 5-Bromo-2′-deoxyuridine (BrdU) (Merck, B5002-100MG). Coverslips were then fixed ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at a concentration of 10×106/100μL in PBS containing 2% FCS (Merck). Staining for cell surface antigens was performed for 20 minutes at 4°C in the dark ...
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bioRxiv - Biophysics 2023Quote: ... 2 mL of supernatant containing viral particles was harvested and filtered with 0.45 μm filter (Merck). Supernatant was immediately used for transduction or stored at -80°C in aliquots.
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bioRxiv - Bioengineering 2021Quote: ... the purified protein containing fraction was concentrated (Centriprep® Centrifugal Filters Ultacel® YM - 50, Merck Millipore).
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bioRxiv - Plant Biology 2022Quote: ... Fractions containing the proteins were concentrated with Amicon® Ultra-15 Centrifugal Filters Ultracel®-100K (Merck-Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... Fractions containing the purified protein were concentrated using a 0.5 ml Amicon Ultra centrifugal filter (Merck Millipore) with a membrane molecular weight cut off 10 kDa.
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bioRxiv - Microbiology 2023Quote: ... Fractions containing purified protein were united and concentrated on Amicon-Ultra-15 10 K filtration units (Merck-Millipore ...
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bioRxiv - Neuroscience 2023Quote: ... Protein-containing fractions were pooled and concentrated on an Amicon 3 kDa MWCO spin concentrator (Merck Millipore). After another IMAC purification step using Ni-NTA resin ...
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bioRxiv - Molecular Biology 2023Quote: ... PsC23 protein-containing fraction was collected and imidazole was removed by 10K centrifugal filter (Merck Millipore, Germany) using buffer (20 mM Tris-Cl ...
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bioRxiv - Cancer Biology 2023Quote: ... Fractions containing target protein were desalted and concentrated on Amicon Ultra 10K centrifugal filter units (Merck Millipore), and the final protein preparation was stored in PBS containing 0.05% Triton X-100 at 4°C.
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bioRxiv - Microbiology 2024Quote: ... Target protein-containing fractions were combined and concentrated using an Amicon Ultra-4 50 kDa filter (Merck) operated at 7,500 x g and 4 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The 1.5 ml resuspended RBCs were cultured in 50 ml HPS containing 2 mM CaCl2 and 2 µM calcium ionophore-4-bromo-A23187 (C7522, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in T75 flasks in a 5% CO2 incubator at 37°C for 16 h ...
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bioRxiv - Systems Biology 2020Quote: ... supplied with 1% Protease Inhibitor Cocktail Set III (Merck Chemicals). After an incubation of 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... supplied with 1% protease inhibitor cocktail set III (Merck Chemicals) for 30 min at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... protease inhibitors (Protease Inhibitor Cocktail set III, EDTA free, Merck), phosphatase inhibitor cocktails (PhosSTOP ...
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bioRxiv - Neuroscience 2023Quote: ... inside a 7 mL KIMBLE Dounce tissue grinder set (Merck), using 10 strokes with loose pestle followed by 10 strokes with tight pestle ...
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bioRxiv - Developmental Biology 2023Quote: ... by using a KIMBLE Dounce tissue grinder set (Merck, #D8938), and immediately frozen in liquid N2.
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bioRxiv - Cell Biology 2021Quote: ... The blocks were then washed with DDW 2 times for 10 min and incubated in 1% (w/v) uranyl acetate (Merck)/70% (v/v ...
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bioRxiv - Cancer Biology 2022Quote: ... special AT-rich sequence binding protein 2 (SATB2; clone EP281, Merck, USA; 1:200), and programmed cell death ligand 1 (PD-L1 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were further concentrated to 2-10 mg/ml using Amicon concentration devices (Merck, 3,000 Da ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg purified recombinant LARK protein or 1 μg BG4 (MABE917, Merck, Darmstadt, Germany) was added ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Neuroscience 2023Quote: ... Separated proteins were wet-blotted (110 V; 90 min; 4°C) on fluorescence-optimized polyvinylidene difluoride (PVDF) membranes (Immobilon-FL; Merck; IPFL00010) in transfer buffer (25 mM Tris (pH 7.6) ...
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bioRxiv - Developmental Biology 2022Quote: ... subsequently the peptides were eluted by 95% (v/v) acetonitrile (Merck, Darmstadt, Germany), 0.1% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 (0.2% v/v; in phosphate buffered saline (PBS)) (Merck, Switzerland) was added to the positive control 15 min prior to collecting the supernatant of each well.
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bioRxiv - Cell Biology 2023Quote: ... After a washing step in 0.05% v/v Tween (Tween 20, 822184, Merck) in PBS (P4417 ...
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bioRxiv - Developmental Biology 2023Quote: ... subsequently the peptides were eluted by 95% (v/v) acetonitrile (Merck, Darmstadt, Germany), 0.1% (v/v ...
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bioRxiv - Plant Biology 2024Quote: ... 5 mM β-mercaptoethanol and 1% (v/v) complete protease inhibitor cocktail (Merck). The protein concentration was quantified in crude extracts with the Bradford assay (Bradford ...
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bioRxiv - Biophysics 2024Quote: ... and 5% glycerol (v/v)) with 25x EDTA-free protease inhibitor cocktail (Merck). The cells were lysed using three cycles of freeze/thawing on dry ice ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Cell Biology 2023Quote: Protein carbonyl formation by oxidative reactions was assessed with the OxyBlot Protein Oxidation Detection Kit (S7150, Merck Millipore), according to manufacturer’s instructions ...