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Citations for Merck :
351 - 400 of 1295 citations for Human Serum Albumin Rabbit Polyclonal affinity purified since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Total IgG were isolated from post-immune sera by affinity purification on Sepharose-protein G (Merck, Cytiva 17-0618-05). Custom sheep pAb anti-pS55 and anti-pS44 NDC80 pAb was raised by the Scottish Blood Transfusion Service using a 4-month long program ...
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bioRxiv - Cancer Biology 2023Quote: ... Immunoprecipitation of cell lysates overexpressing FLAG-tagged or GST-tagged proteins were performed using anti-FLAG-M2 affinity beads (Merck) or glutathione-Sepharose beads (Cytiva).
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Microbiology 2024Quote: ... 500µg of cell lysates were incubated with pre-washed EZview Red ANTI-HA or FlagM2 affinity Gel Beads (E6779 and F2426, Merck) at 4 °C under overnight rotation ...
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bioRxiv - Immunology 2024Quote: ... Clear whole-cell extracts were prepared by centrifugation at 20000g for 20min at 4°C and incubated with EZview Red Anti-c-Myc-Affinity gel (Merck) for overnight ...
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bioRxiv - Biochemistry 2023Quote: ... Digests were purified with C18 ZipTips (Merck) and dried ...
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bioRxiv - Microbiology 2023Quote: ... A range of purified toxin A (Merck) from 0 to 1 μg/ml used to perform a standard curve and dilutions of the samples were added to the wells ...
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bioRxiv - Neuroscience 2022Quote: ... and a polyclonal antibody against detyrosinated tubulin (1:1000; AB3201, Merck Millipore, Darmstadt, Germany) were used ...
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bioRxiv - Cancer Biology 2023Quote: ... The primary antibodies guinea pig polyclonal anti-doublecortin antibody (Merck, AB2253, 1:200 dilution), mouse monoclonal anti-CKAE1/AE3 (Dako ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with F(ab’)2-Goat anti-Human IgG-FITC (Merck Goat anti-Human IgG, F(ab’)2 ...
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bioRxiv - Physiology 2021Quote: Human thrombin was purchased from Merck (Cramlington, UK) and reconstituted in double distilled water (ddH2O) ...
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bioRxiv - Cell Biology 2022Quote: ... and anti-human nuclei (Merck, MAB4383, 1:250) were diluted in the blocking solution and incubated overnight on a shaker at 4◦C ...
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Kinetochore- and chromosome-driven transition of microtubules into bundles promotes spindle assemblybioRxiv - Cell Biology 2022Quote: ... human Ndc80 siRNA (HA12977117-004; Merck, Darmstadt, Germany), human ON-TARGET PRC1 siRNA (L-019491-00-0020 ...
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bioRxiv - Developmental Biology 2021Quote: ... 0.1% aprotinin and 0.1% Human Insulin Solution (Merck). Medium was changed every other day with EHT medium.
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bioRxiv - Cancer Biology 2019Quote: ... mouse anti-human nuclear antigen (1:100; Merck), Alexa Fluor 647-conjugated rat anti-CD45 (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HTF medium (EmbryoMax Human Tubal Fluid; Merck Millipore) was mixed 1:1 with mineral oil (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: ... Normal human epidermal keratinocytes were obtained from Merck. All lines were maintained in 3:1 DMEM (Gibco ...
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bioRxiv - Cancer Biology 2019Quote: ... 10% fetal bovine serum (FBS; Merck, Darmstadt, Germany), 2 mM L-Glutamine (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... and 25% heat-inactivated horse serum (Merck-Sigma). Media was exchanged one day after preparation and subsequently every three days ...
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bioRxiv - Cancer Biology 2021Quote: ... Blocking was done with 10% donkey serum (Merck). Primary antibody (S139 ...
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bioRxiv - Neuroscience 2020Quote: ... with 10% Goat serum (S26-100ML, Merck Millipore) and 2% BSA (B9000S ...
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bioRxiv - Neuroscience 2020Quote: ... with 1% Goat serum (S26-100ML, Merck Millipore) and 0.2% BSA (B9000S ...
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bioRxiv - Evolutionary Biology 2020Quote: ... supplemented with 10% fetal bovine serum (Merck, Germany), at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... and 10% donkey serum (Merck, cat.566380-10ML) for one hour ...
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bioRxiv - Cell Biology 2021Quote: ... and further blocked in 10 % donkey serum (Merck). Cells were incubated with unlabeled primary antibodies ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal bovine serum (FBS; Merck), 50 U/ml of penicillin ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% fetal bovine serum (FBS, Merck), 1% 100× MEM Non-essential Amino Acid Solution (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... 1% DMSO and 5% normal goat serum (Merck) for 1 h at RT and incubated overnight with primary antibodies ...
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bioRxiv - Molecular Biology 2022Quote: ... supplemented with 10% fetal bovine serum (FBS; Merck) and 5% penicillin/streptomycin (Merck) ...
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bioRxiv - Cell Biology 2023Quote: ... supplied with 10% fetal bovine serum (FBS, Merck). eHAP cells were purchased from Horizon Discovery (C669 ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10% fetal calf serum (Merck, Germany) and were lysed with SingleShot lysis buffer (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: ... resuspended using fetal bovine serum (FBS, F7524 Merck) 10% DMSO to freeze a total of ∼2×106 cells per cryovial in 1ml volume were aliquoted ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 10% fetal bovine serum (FBS, Merck), 100U/ml Penicillin and 100μg/ml Streptomycin (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 5 % heat-inactivated horse serum (Merck), EGF (20 ng mL−1 ...
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bioRxiv - Neuroscience 2023Quote: ... + 5% HS + 5% donkey serum (DS, Merck - D9663) at RT for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Blocking solution containing 10% donkey serum (Merck, D9663), 5% BSA (Millipore,821006) ...
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bioRxiv - Biophysics 2024Quote: ... containing 10% foetal bovine serum (Sigma-Aldrich, Merck) and 1% penicillin-streptavidin (Gibco ...
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bioRxiv - Immunology 2024Quote: ... supplemented EX-CELL 420 serum-free media (Merck).
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were clarified by centrifugation and a minimum of 200μg of lysate was incubated with pre-washed EZview™ Red ANTI-FLAG® M2 Affinity Gel (F2426, Merck) at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilised mitochondria were then clarified by centrifugation at 16,000 g for 10 min and incubated with pre-washed anti-Flag® M2 Affinity Gel (Merck; A2220) at 4 °C for 2 hours ...
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bioRxiv - Cell Biology 2022Quote: ... The eluate of the affinity purification was concentrated to a volume between 1-2 mL before injection using centrifugal filter units (Amicon, Merck millipore) with a MWCO of 10 kDa ...
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bioRxiv - Cell Biology 2020Quote: ... from affinity purification were pooled and buffer exchanged using Amicon™ Ultra-15 Centrifugal Filter Unit (10 kDa cutoff; Merck™) in 10 mM HEPES-NaOH buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The eluate of the affinity purification was concentrated to a volume between 1-2 mL before injection using centrifugal filter units (Amicon, Merck millipore) with a MWCO of 5,000 Da ...
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bioRxiv - Biochemistry 2023Quote: ... the Streptag-purified protein after removal of the C-terminal affinity-GFP-tag was collected and concentrated using 100 kDa MW cut-off spin concentrator (Amicon Merck-Millipore) further purified by size-exclusion chromatography (SEC) ...
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bioRxiv - Cell Biology 2023Quote: ... Similar lysis buffer conditions were used for HA-based affinity purification of SHA-BAG2 in presence and absence with 20 μM of the ULK1 inhibitor MRT68921 (Merck, SML1644) (2 h prior HBSS treatment ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Molecular Biology 2023Quote: ... stained with anti-human IFN-γ and anti-human IL2 antibodies for 40 min at RT in 0.2% saponin (Merck, Germany) in PBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG, #A0545). Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).