Labshake search
Citations for Merck :
451 - 500 of 3878 citations for Human Putative Alpha 1 Antitrypsin Related Protein SERPINA2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
McIdas localizes at centrioles and controls centriole numbers through PLK4-dependent phosphorylationbioRxiv - Molecular Biology 2022Quote: ... Proteins were analyzed by SDS-PAGE and transferred onto PVDF membrane (Merck Millipore). The membranes were washed with PBS 1x containing 0.1% Tween-20 (PBS-T ...
-
bioRxiv - Molecular Biology 2022Quote: ... The purified protein was concentrated using an Amicon Ultra 100 K filter (Merck Millipore ...
-
bioRxiv - Microbiology 2022Quote: ... and the FLAG-tagged proteins were immunoprecipitated with anti-FLAG agarose (A2220, MERCK). Input samples were collected as described above.
-
bioRxiv - Developmental Biology 2023Quote: ... and separated proteins were then transferred to PVDF membranes (polyvinylidene difluoride, Merck Millipore). After treatments with a blocking reagent (Blocking One solution ...
-
bioRxiv - Molecular Biology 2023Quote: Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore) utilizing a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) ...
-
bioRxiv - Molecular Biology 2023Quote: ... Proteins separated by SDS-PAGE were transferred to Immobilon-P membranes (Merck Millipore). Three major bands on the membrane were cut and subjected to N-terminal Edman sequencing by Hokkaido System Science Co. ...
-
bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Amersham, MERCK). Membranes were blocked with Odyssey Blocking Buffer (LI-COR ...
-
bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
-
bioRxiv - Plant Biology 2023Quote: ... Protein pellets were resuspended in 50 µl of 2.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
-
bioRxiv - Biochemistry 2023Quote: ... Then the protein was concentrated with centrifugal concentrators (UFC901096, Merck Millipore, Cork, Ireland), and loaded onto a SuperoseTM 6 Increase 10/300 GL gel-filtration column (29- 0915-96 ...
-
bioRxiv - Cell Biology 2023Quote: Saporin was labelled with an ATTO 550 protein labelling kit (Merck, 51146-1KT).
-
bioRxiv - Cell Biology 2023Quote: Wet blot transfer was used to transfer proteins to a PVDF membrane (MERCK). Intercept blocking buffer (927-60001 ...
-
bioRxiv - Molecular Biology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated with first and secondary antibodies ...
-
bioRxiv - Cancer Biology 2023Quote: ... Proteins were resolved in SDS-PAGE gels and electrotransferred onto nitrocellulose membranes (Merck). After incubation with the appropriated primary antibody ...
-
bioRxiv - Microbiology 2023Quote: ... Then the protein sample was concentrated using an Amicon Ultra centrifugal tube (Merck) with a buffer change to phosphorylation buffer 2X (100 mM Tris pH 8 ...
-
bioRxiv - Plant Biology 2023Quote: ... after concentrating the protein (Amicon® Ultra MWCO 30 kDa, Merck, Darmstadt, Germany), a second GST-tag purification was performed and the peak fractions were pooled and concentrated (Amicon® Ultra MWCO 10 kDa ...
-
bioRxiv - Plant Biology 2023Quote: ... proteins were blotted on the Immobilon-P PVDF 0.45 µm (MERCK, Darmstadt, Germany). PVDF membranes were blocked using the PVDF Blocking Reagent for Can Get Signal (TOYOBO) ...
-
bioRxiv - Microbiology 2023Quote: ... and the protein concentration was determined by bicinchoninic acid (BCA) assay (Merck Millipore) and by UV-visible spectroscopy using an ε of 24 mM⁻¹·cm⁻¹ at 280 nm ...
-
bioRxiv - Physiology 2023Quote: ... and protein concentration in the supernatant was determined by BCA assay (Merck B9643). Finally ...
-
bioRxiv - Biochemistry 2023Quote: ... The 139H2 was purified with Protein G Sepharose 4 Fast Flow beads (Merck), washed with PBS ...
-
bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
-
bioRxiv - Microbiology 2023Quote: ... The proteins were concentrated by centrifugal filters (Amicon Ultra 10K, Merck Millipore Ltd.). Protein concentrations were determined by a Pierce™ BCA Protein Assay Kit (Thermo Fischer Scientific ...
-
bioRxiv - Biochemistry 2023Quote: ... the proteins were filtered using an Ultrafree 0.22 μm centrifugal filter (Merck Millipore) to remove any aggregation ...
-
bioRxiv - Cancer Biology 2023Quote: ... and 50 µM Rho-associated protein kinase (ROCK) inhibitor (Merck Millipore, SCM-075). On day 3 ...
-
bioRxiv - Cell Biology 2023Quote: ... the proteins were transferred to 0.45 µm PVDF membranes (Merck Millipore, cat.no. IPVH00010). After blocking unspecific binding sites of the membrane at RT for 1 h with 5 % skim milk powder in TBS-T ...
-
bioRxiv - Cell Biology 2023Quote: Caco-2 cells were co-transfected with eSpCas9-GFP protein (Merck, cat #ECAS9GFPPR) and sygRNA (Merck ...
-
bioRxiv - Pathology 2023Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
-
bioRxiv - Microbiology 2024Quote: ... The pellet was resuspended in protein resuspension buffer (150 mM NaCl (Merck, USA), 5 mM EDTA (Merck ...
-
bioRxiv - Plant Biology 2024Quote: ... Protein pellets were resuspended in 50 μl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
-
bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... Eluted proteins were concentrated using a 10K Amicon tube (Merck-Millipore, Darmstadt, Germany) and the buffer was changed to PBS ...
-
bioRxiv - Molecular Biology 2024Quote: ... FLAG-tagged proteins were detected using M2 anti-FLAG antibody (mouse, F1084, Merck) followed and fluorescent Anti-Mouse Alexa Fluor™ Plus 800 ...
-
bioRxiv - Biochemistry 2024Quote: ... Immunoreactive proteins were visualized using Immobilon® Forte Western HRP Substrate (Merck Millipore) at the Imager Fluor-Chem M (Proteinsimple) ...
-
bioRxiv - Molecular Biology 2024Quote: ... protein pellets were resuspended in 50 µl of 1.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
-
bioRxiv - Cell Biology 2023Quote: ... anti-human kindlin2 (mouse monoclonal, 3A3, Merck, MAB2617; WB 1:1000, IF 1:200), anti-mouse kindlin2 (rabbit polyclonal ...
-
bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
-
bioRxiv - Neuroscience 2019Quote: ... cortical sections were incubated overnight at 4 °C with anti-glial fibrillary acidic protein (GFAP, 1:1000, chicken IgG, Merck Millipore, Burlington, EUA), anti-Iba1 (1:200 ...
-
bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
-
bioRxiv - Molecular Biology 2023Quote: ... Relative levels of pFlareG were calculated for each condition using GAPDH as a housekeeping gene and normalized relative to FLAG-PTBP2 protein levels immunoprecipitated checked by western blot using α-FLAG M2 antibody (1:500; Merck-Sigma, F3165) and IRDye 800RD (1:10000 ...
-
bioRxiv - Molecular Biology 2023Quote: ... and anti-alpha sarcomeric actin clone 5C5 (#2172, Sigma-Aldrich, Merck Darmstadt, Germany) (1:500 v/v dilution in 5% BSA in PBS tween 0.05%) ...
-
bioRxiv - Cancer Biology 2021Quote: ... and thereafter protein transferred onto 45 µm Polyvinylidene difluoride (PVDF) membrane (IPVH00010, Merck Millipore). Western blotting analysis was performed by using specific primary and secondary antibodies and signals visualized with chemiluminescence ...
-
bioRxiv - Cell Biology 2020Quote: ... and nucleoproteins were extracted using a nuclear protein extraction kit (Merck Millipore, Darmstadt, Germany). Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS ...
-
bioRxiv - Cell Biology 2019Quote: ... Supernatant containing Matrigel proteins was discarded and pellets were lyzed in RIPA buffer (Merck), supplied with protease and phosphatase inhibitors (Merck) ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... For IP with guinea-pig antibody protein A-conjugated magnetic beads (Merck Millipore, LSKMAGA10) were used ...
-
bioRxiv - Developmental Biology 2020Quote: hESCs were harvested for protein extraction by addition of CelLytic M lysis buffer (Merck), freshly supplemented with protease inhibitors (PIC ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were concentrated with an Amicon Ultra-15 10 kDa centrifugal filter unit (Merck). Protein concentration was determined by UV-spectroscopy.
-
bioRxiv - Immunology 2021Quote: ... Supernatants were first precleared with PureProteome™ Protein A/G agarose suspension (Merck Millipore) for 1 h ...
-
bioRxiv - Biochemistry 2020Quote: ... The purified proteins were then concentrated on either Amicon (Merck Millipore, MWCO 10 kDa) or Vivaspin (Sartorius ...
-
bioRxiv - Genetics 2021Quote: ... Proteins separated by SDS-PAGE were transferred onto a PVDF membrane (Immobilon-E, Merck) and blocked in 5% milk in Tris-Buffer Saline (TBS ...
-
bioRxiv - Pathology 2021Quote: ... proteins were transferred from the gel onto to a polyvinylidene fluoride membrane (Merck Millipore) via electroblotting at 120 V for 60 minutes in transfer buffer containing 20% methanol ...