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Citations for Merck :
151 - 200 of 666 citations for Histone H3 mono methyl K36 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: HaCaT mCherry-Histone H2B cells were seeded in 96-well glass bottom plates (Greiner Sensoplate (M4187-16EA, Merck)) at 75 000 cells per well ...
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bioRxiv - Biochemistry 2023Quote: ... the reduced cysteine residues were blocked employing a 10 mM solution of S-methyl methanethiosulfonate (MMTS) (Merck KGaA, Darmstadt, Germany) at room temperature for 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... the GFP-Trap bead bound fraction was incubated with recombinant TEV (Merck) overnight at 4°C ...
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were treated with recombinant mouse TNFα (10 ng/ml, Merck 654245).
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bioRxiv - Developmental Biology 2023Quote: ... then 1,000 IU recombinant human chorionic gonadotropin alpha (rhCG, OVIDREL, Merck Serono) was injected on day 9 ...
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bioRxiv - Plant Biology 2023Quote: ... the Immunoprecipitation (IP) was performed following a standard IP protocol and using the following antibodies: H3 (Reference: 07-690, Merck), H3K9me2 (Reference ...
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bioRxiv - Plant Biology 2023Quote: ... the Immunoprecipitation (IP) was performed following a standard IP protocol and using the following antibodies: H3 (Reference: 07-690, Merck), H3K9me2 (Reference ...
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bioRxiv - Cell Biology 2020Quote: ... H4S1 phosphorylation was detected using the anti-phospho histone H4/H2A S1p antibody at 1:1000 (Upstate/Merck-Millipore); sf-GFP-Sps1 was detected using JL-8 anti-GFP antibodies (Takara/Clontech ...
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bioRxiv - Developmental Biology 2021Quote: ... plus 5 % sheep serum in MAB and incubated with anti-DIG conjugated to Peroxidase (1/50; Merck) in 1 % blocking reagent diluted in MAB for 3 days at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... NBP1-92693AF647) and the oligodendrocyte transcription factor OLIG2 (Anti-OLIG2 clone 211F1.1 mouse mAb, Merck Millipore, MABN50A4). Samples were kept on ice throughout the isolation and staining procedure ...
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bioRxiv - Microbiology 2023Quote: ... the solution was incubated at 30 °C for 90 min and then derivatized with 20 µl of N-methyl-N-trimethylsilyltrifluoroacetamide with 1% trimethylchlorosilane (Merck, USA) at 70 °C for 30 min54 ...
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bioRxiv - Cell Biology 2022Quote: ... DLD-1-WT mCherry-Sec61β and DLD-1-C-H3 mCherry-Sec61β stable cell lines were generated by GeneJuice (Merck Millipore) transfection of mCherry-Sec61β into the respective parental lines ...
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bioRxiv - Microbiology 2023Quote: ... and a 1:500 dilution of an antibody against the phosphorylated form of H2A histone family member X (γH2Ax) (clone JBW301, Merck) and 1:200 phalloidin coupled to Alexa Fluor 568 ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies against target histone modifications were used to detect respective bands (Anti H3K27Me3 #9733 Cell Signaling, Anti H3K9Me #ABE101 Merck Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... then the mixture was dialyzed against histone refolding buffer at 4 □ using a dialysis tube (D-TubeTM Dialyzer Medi or Maxi, MWCO 6-8 kDa, Merck). HE buffer (10 mM HEPES ...
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bioRxiv - Genetics 2021Quote: Before the ovaries were stimulated with recombinant FSH (Gonal-F, Merck Serono, Switzerland), downregulation was performed using a GnRH agonist (Decapeptyl ...
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bioRxiv - Zoology 2020Quote: ... Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck) in TE buffer (10 mM Tris-HCI ...
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bioRxiv - Bioengineering 2021Quote: ... recombinant human platelet derived growth factor (hPDGF-AB, 20 ng/ml, Merck, GF106), recombinant human oncostatin M (hOSM ...
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bioRxiv - Cell Biology 2022Quote: All experiments were performed using the BDNF mature recombinant (GF029; Merck Millipore, USA) or the C-ter truncated form of BDNF (BDNFΔC-ter) ...
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bioRxiv - Neuroscience 2023Quote: ... and human recombinant FGF-2 (10 ng/ml; Merck Millipore, Burlington, MA, USA) as mitogens ...
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bioRxiv - Immunology 2023Quote: ... Mo were primed with 200 U/mL human recombinant IFNα2β (Intron A; Merck) for 2 h and then stimulated with 20 ng/mL of recombinant human mIL-1β (R&D ...
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bioRxiv - Biophysics 2020Quote: ... Refolded I-Ek/MCC(ANP)-biotin complexes were purified via 14.4.4S mAb-based affinity chromatography (Cyanogen bromide-activated Agarose, MERCK) followed by gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Immunology 2023Quote: The I-Ek-specific 14.4.4 mAb was purified from hybridoma supernatant using a protein A/G column (Merck) and gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Microbiology 2023Quote: ... 50 µl of each dilution were mixed with 10 µl of 0.4 mg ml-1 4-methyl-umbelliferyl-β-D- galactopyranoside (MUG) substrate (Merck, Darmstadt, Germany) that was prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Biochemistry 2024Quote: ... Samples were derivatized for 1 hour at 85 °C using N-tert-Butyldimethylsilyl-N-methyl-trifluroacetamide with 1% tert-Butyldimethylchlorosilane (Merck, NJ, USA) and immediately analysed by GC-MS ...
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bioRxiv - Genomics 2019Quote: ... We sequenced two biological and two technical replicates per isolate and used antibodies against the euchromatin histone mark H3K4me2 (#07–030, Merck Millipore). Sequencing was performed at the OSU Center for Genome Research and Biocomputing (Oregon State University ...
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bioRxiv - Genomics 2019Quote: ... ChIP was performed on this chromatin extract using 1 µL of Anti-acetyl-Histone H4 (Lys16) Antibody (Merck Milipore, 07-329) or 0.5 µL of Histone H3 antibody mAb MABI 0301 (Active Motif ...
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bioRxiv - Biophysics 2023Quote: ... and fractions containing histone octamers were pooled and concentrated in an Amicon Ultra-4 Ultracell 30kDa centrifugal filter (Merck-Millipore #UFC803024). The protein concentration was determined with Bio-Rad Protein Assay Dye Reagent (Bio-Rad # 5000006).
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bioRxiv - Cancer Biology 2023Quote: ... was added for 16-18 hr at 4°C before washes and the addition of anti-phospho H2A.X mAb (Merck, Kenilworth ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Brains were placed into 100 μL of sodium acetate buffer (pH 5.0) containing the internal standard alpha-methyl dopamine (αMDA; Merck & Co., Inc., Kenilworth, NJ). Fish bodies were placed in either 200 to 400 μL of acetate buffer containing αMDA based on the amount of tissue present ...
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bioRxiv - Bioengineering 2019Quote: ... The supernatant was passed through the cartridges (pre-conditioned with 5 mL each of tert-methyl butyl ether, methanol (Merck; laboratory grade purity) and deionised water ...
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bioRxiv - Molecular Biology 2019Quote: ... protocol with the administration of recombinant FSH (Gonal-F, Merck Serono, or Puregon, Merck Sharp & Dohme Corp. ...
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bioRxiv - Molecular Biology 2023Quote: ... 2 μg purified recombinant LARK protein or 1 μg BG4 (MABE917, Merck, Darmstadt, Germany) was added ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant proteins were purified with His-Bind Resin and His-Bind Buffers (Merck Millipore) according to manufacturer’s protocol and stored at a concentration of 2mg/ml in elution buffer (Merck Millipore ...
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bioRxiv - Plant Biology 2023Quote: Non-histone ChIP-seq was performed as described above with the modifications that rProtein A Sepharose Fast Flow (Merck, GE17-1279-01) was used instead of Protein A Agarose/Salmon Sperm DNA to avoid Salmon sperm DNA contamination ...
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bioRxiv - Developmental Biology 2024Quote: ... For each IVF session one epididymis from 12-week-old WT and one from Trim66-null were transferred into 90 μL of capacitation medium, consisting of TYH (Takeo & Nakagata, 2011) with 0.75 mM methyl-β-cyclodextrin (MBCD, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany). Spermatozoa were allowed to disperse from the tissue and incubated for 30 min in a 5% CO2 incubator at 37°C.
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bioRxiv - Molecular Biology 2021Quote: ... Smchd1 deletion was confirmed by immunofluorescence with an in-house made anti-Smchd1 antibody during the immunofluorescence experiments (Mab #8, now available commercially from Merck).
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bioRxiv - Bioengineering 2020Quote: ... or mouse monoclonal β-actin antibody (1:1000 dilution; catalog no. catalog no. MAB 1501; Merck Millipore, Burlington, MA, USA) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: 400 μg of purified GABAAR mAbs were concentrated to a volume of 100 μl using Amicon Ultra 100 kDa columns (Merck) and then incubated with 40 μg CruzFluor™ 488 (CF488 ...
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bioRxiv - Molecular Biology 2022Quote: ... rabbit IgG (Merck), (Domanski et al. ...
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Dual signaling via interferon and DNA damage response elicits entrapment by giant PML nuclear bodiesbioRxiv - Microbiology 2021Quote: ... PAb directed against phospho-STAT2 (Tyr689) and MAb directed against human IFNα/β receptor chain 2 (MAB1155) were purchased from Merck-Millipore.
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bioRxiv - Cancer Biology 2019Quote: The uncharged polystyrene 96-well plate were coated with different recombinant proteins-Collagen IV (Merck Millipore), P-Selectin ...
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bioRxiv - Biophysics 2022Quote: ... Both recombinant and synthetic lyophilized peptides were dissolved in 6M guanidine thiocyanate (Merck KGaA, Darmstadt, Germany) and incubated overnight at 1250 rpm ...
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bioRxiv - Genetics 2020Quote: ... which was performed by intramuscular injection with rhFSH (recombinant human follitropin alpha, GONAL-F, Merck Serono) for 8 days ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant proteins were obtained after expression at 22°C in B834 (DE3) cells (Merck, Darmstadt, Germany), upon induction with 0.6□mM IPTG at 0.8 OD600 ...
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bioRxiv - Immunology 2023Quote: ... The eluted His-Myc tagged recombinants were further concentrated using 3kDa ultra concentration units (Merck, Germany). The GST-tagged recombinant proteins were affinity purified using Glutathione Sepharose 4B beads and purified in batch mode ...
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bioRxiv - Molecular Biology 2022Quote: ... The beads were pre-bound with 4-5 micrograms of antibodies (H3K4me3 – Merck 07-473; H3K9me2 Abcam ab1220; H3K27me3 – Acive Motif 39155; H3 – Merck 07-10254; PolII – Abcam ab817) before incubation with the sheared chromatin ...
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bioRxiv - Immunology 2023Quote: ... and 1:80,000 for Anti-Rabbit antibody (Merck Anti-Rabbit IgG, #A0545). Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-PRRX16 (1:200) rabbit anti-Laminin (Merck, #L9393 1:200). Secondary antibodies used in these studies were procured from Thermo Fisher Scientific (Waltham).