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Citations for Merck :
51 - 100 of 3970 citations for HLA A*0201 WT 1 complex Protein Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells were incubated with 50 µM 17ODYA (17-octadecynoic acid, Merck) added from stock solution in DMSO or with 0.05% DMSO in DMEM containing 2% charcoal-stripped FBS and 30 mM HEPES for 4 h at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Tissues were directly immersed in CUBIC-L solution (10 wt % N-Butyldiethanolamine [Tokyo Chemical Industry, B0725], 10 wt % Triton X-100 [Merck, 1.08603.1000] and 80 wt % MilliQ water) ...
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bioRxiv - Systems Biology 2020Quote: Agarose pads were made by pipetting 1 ml of 1.5 % (wt/vol) low melt agarose (Merck, Darmstadt, Germany) dissolved in PBS onto a 22 mm2 cover glass slide ...
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bioRxiv - Immunology 2023Quote: Resuspended HLA-I-eluted samples were centrifuged through 5 kDa cutoff filters (Merck Millipore #UFC3LCCNB-HMT) and vacuum-dried ...
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bioRxiv - Immunology 2021Quote: ... anti-human Caspase-1 antibody (06-503, Merck Millipore); anti-mouse IL-1β antibody (5129-100 ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore), and anti-E-cadherin-1 (ECAD ...
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bioRxiv - Microbiology 2020Quote: ... coli onto a fresh plate of complex solid media (Merck LB 110283) containing selective antibiotics when appropriate ...
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bioRxiv - Developmental Biology 2024Quote: ... the ARP2/3 complex inhibitor CK666 (Merck, 100 μM, 60-min incubation); the actin polymerization inducer and actin depolymerization inhibitor jasplakinolide (Abcam ...
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A small molecule reveals role of insulin receptor-insulin like growth factor-1 receptor heterodimersbioRxiv - Cell Biology 2021Quote: HEK293 cells were cultured in Dulbecco⍰s Modified Eagle⍰s Medium (DMEM, Merck) containing 10% Fetal Calf Serum and 1% Antibiotic Antimycotic Solution (Sigma Aldrich) ...
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bioRxiv - Plant Biology 2022Quote: ... the CDS of wild type (WT) PpGS1b.1 and PpGS1b.2 were subcloned into pET30a vector (Merck, Darmstadt, Germany) including a N-terminal 6xHis-tag by PCR ...
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bioRxiv - Bioengineering 2023Quote: ... the chips were coated with fibronectin (Human Plasma Fibronectin Purified Protein, Merck, Schiphol-Rijk, The Netherlands) in sterile PBS (100 μg/mL ...
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bioRxiv - Biophysics 2021Quote: ... Purified complex was concentrated with an Amicon Ultra 30 K unit (Merck Millipore).
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bioRxiv - Microbiology 2020Quote: ... A single colony was then incubated in liquid complex media (Merck LB 110285) overnight cultures ...
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bioRxiv - Molecular Biology 2022Quote: ... The complex was concentrated using ultrafiltration through a 100 kDa cutoff filter (Merck) and analyzed on a 10% SDS PAGE for the presence of Cas subunits ...
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bioRxiv - Biophysics 2021Quote: ... 6×His-tagged recombinant MSP1E3D1 (Sigma-Aldrich, Merck, USA) and FLAG-tagged recombinant mCardinal (kindly provided by Jakub Czapiński ...
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bioRxiv - Biochemistry 2024Quote: ... we utilized the His-Bind purification kit (Merck, USA) followed by manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... anti-human kindlin2 (mouse monoclonal, 3A3, Merck, MAB2617; WB 1:1000, IF 1:200), anti-mouse kindlin2 (rabbit polyclonal ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 10 ng/ml of the recombinant human TNFα protein (Merck-Millipore, Cat# GF023) and the outcome was assessed at RNA or protein levels.
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bioRxiv - Bioengineering 2020Quote: ... and the membrane was incubated with primary antibody (anti-his tag, 1:1000, Merck Millipore, Merck KGaA, Darmstadt, Germany) diluted in 2 % skim milk for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Biophysics 2024Quote: ... containing 44.3 weight percentage (wt.%) polyvinylpyrrolidone-40 (PVP-40, Merck & Co.) and 1.7 wt.% sodium chloride (NaCl ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with a mouse anti-DENV complex monoclonal antibody (MAB8705, Merck Millipore), washed three times with PBS ...
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Lowering mutant huntingtin by small molecules relieves Huntington’s disease symptoms and progressionbioRxiv - Molecular Biology 2023Quote: ... and anti-eIF4G1 for human samples (1:2000; 07-1800, Merck) or anti-eIF4G3 for mouse samples (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibodies directed against anti-human mitochondria (1:100, Merck #1273;), CDK2AP1 (rabbit ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 cells seeded in 6-well plates or 100mm dishes were transfected using GeneJuice (Merck) and 1 to 3µg DNA ...
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: Glass bottom well plates (Celvis) were coated with 5 µg/ml of human plasma fibronectin purified protein (Merck) for one hour at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... WT SETDB1 was amplified by KOD Hot Start DNA Polymerase (71086, Merck) and the oligonucleotides 5’-cagagctcATGTCTTCCCTTCCTG GGTGCAT-3’ and 5’-gtgtcgaCTAAAGAAGACGTCCTCTGCATTCAAT-3’ ...
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bioRxiv - Cell Biology 2023Quote: ... hTERT-RPE1-Flp-In-Parental cells were co-transfected with a 10:1 ratio of pOG44 and either pCDNA5 FRT/TO-NeoR-HA-VPS35-WT or -[D620N] using Genejuice (Merck Millipore; 70967), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The purified complex I was concentrated using an Amicon Ultra-4 100K centrifugal filter (Merck) to 0.5 mg mL-1 (w/v) ...
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bioRxiv - Biochemistry 2022Quote: ... mPSF:PAP complex was concentrated in centrifugal filter (Amicon Ultra-15, MWCO 100 kDa, Merck Millipore), aliquoted ...
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bioRxiv - Molecular Biology 2020Quote: ... The TDH complex was concentrated in a 100 kDa cut-off Amicon centrifugal concentrator (Merck) at 1,500 rcf to 0.1-0.2 mg/ml ...
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bioRxiv - Biochemistry 2022Quote: ... The AstaPo1/AXT complex was concentrated by an Amicon concentrator with 3.5 kDa MWCO (Merck) to a concentration of 7 mg/mL (280 μM).
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bioRxiv - Cell Biology 2023Quote: ... the reducing agent 2 µl 2-picoline borane complex (PB, 2 M in DMSO, Merck) and 2 µl water ...
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bioRxiv - Immunology 2023Quote: ... MDMs were also pre-treated for 1h with a TLR3/dsRNA complex inhibitor (Merck Millipore) 5 µM final concentration ...
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bioRxiv - Biochemistry 2023Quote: ... instead the pyrene–actomyosin complex was incubated with 30 µM ADP (Merck KGaA, Darmstadt, Germany) for 20 minutes on ice prior to measurement ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mg/l hydrocortisone and 1 µg/l and human epidermal growth factor (hEGF) (both Merck). To determine EC sex ...
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bioRxiv - Immunology 2022Quote: For protein crystallization “empty” HLA-DPA1*02:01-DPB1*01:01 was purified on a Superdex 75 10/300 GL column (Merck) into 20mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2021Quote: ... The binding of CoVHH1-FLAG to S1 subunits-His tag was detected by incubating for 1 hour at room temperature with mouse monoclonal ANTI-FLAG® M2-Peroxidase (Merck) diluted with PBST ...
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bioRxiv - Biophysics 2021Quote: ... anti-6×His and anti-FLAG M2 (Sigma-Aldrich, Merck, USA). The anti-mouse IgG ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was added to a His Trap HP column (Merck) previously equilibrated with buffer A ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Merck). Immortalized human C20 microglia were kindly provided by David Alvarez-Carbonell (21) ...
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bioRxiv - Plant Biology 2022Quote: ... loaded onto His-Bind® Resin columns (EMD Millipore; Merck KGaA), and the recombinant ZmEREB57 protein was eluted with elution buffer (0.5 M NaCl ...
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bioRxiv - Neuroscience 2020Quote: ... 1:50 Hyaluronan binding protein HABP (#H0161; Merck, Germany) or 1:100 mouse anti-Neurocan (#N0913 ...
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bioRxiv - Molecular Biology 2023Quote: NoDice FHA:DICER WT and N1 cells were treated with BAY 11-7082 (Merck) by replacing the culture medium with a medium containing the indicated BAY 11-7082 concentrations at 1µM or 5µM or the corresponding volume of DMSO only in the control conditions ...
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bioRxiv - Immunology 2020Quote: ... Peptides were separated from the HLA molecule remnants by ultrafiltration employing 3 kDa and 10 kDa Amicon filter units (Merck Millipore) for HLA-I and HLA-II ...
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bioRxiv - Biochemistry 2022Quote: ... the complex was concentrated using a 30 kDa MWCO Amicon Ultra Centrifugal Filter Unit (Merck Millipore) and purified by gel filtration on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated with filipin complex (Merck, cat #F-9765; 0.15 mg/ML in D-PBS) for 2 h and after several washes in D-PBS were visualized by fluorescence microscopy ...