Labshake search
Citations for Merck :
151 - 188 of 188 citations for GC TEMPase 2x Master Mix II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... Myosin II filaments were further diluted in KMEH to 200 nM and added to the actin filaments bound to the SLB by His6-Curly by replacing 1/10 of the sample buffer with the myosin II filament solution and supplemented with 0.1 mM Mg-ATP as well as a mix of 1 mM Trolox (cat. no. 648471, Merck), 2 mM protocatechuic acid (cat ...
-
bioRxiv - Cell Biology 2022Quote: MCF10 DCIS.com (DCIS.com) lifeact-RFP cells (Riedl et al., 2008) were cultured in a 1:1 mix of DMEM (Merck) and F12 (Merck ...
-
bioRxiv - Cancer Biology 2019Quote: ... was used together with TransLenti Viral Packaging Mix (Open Biosystems) and Genejuice (Novagen, Merck Life Science AS, Oslo, Norway) to transfect 293T packaging cells (Open Biosystems ...
-
bioRxiv - Plant Biology 2024Quote: Callus inducing media was prepared as follows: 3.2gr of Murashige and Skoog B5 (MS) media mix (G5893, Sigma-Merck), 0.5gr of MES salt (M8250 ...
-
bioRxiv - Cell Biology 2020Quote: Epithelial canine MDCK II (CRL2936) cells were obtained from the ATCC and grown in MEM supplemented with 5% FBS (Merck) at 37°C in an atmosphere of 5% CO2 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Eluted fractions containing HCA II were pooled and concentrated to ~20 mg/ml by using Amicon Ultra Centrifugal Filter Units (Merck) with a molecular weight cut-off 10 kDa.
-
bioRxiv - Molecular Biology 2022Quote: ... The following antibodies were used for ChIP analysis: Mouse anti-RNA polymerase II antibody clone CTD4H8 (Merck Millipore, 05-623), Rabbit anti-NF-kB p65 antibody clone D14E12 (Cell Signalling ...
-
bioRxiv - Microbiology 2024Quote: ... 10 μM of BPP43_05035 was subjected to incubation with 0.5% porcine gastric mucin II or III (Merck, #M2378 and #M1778) in reactions conducted under pH 5.5-8.5 ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were mounted on slides and covered with an anti-fading medium using a mix solution 1:10 Propyl-gallate:Mowiol (P3130, SIGMA-Aldrich, Madrid, Spain; 475904, MERCK-Millipore ...
-
bioRxiv - Plant Biology 2021Quote: ... Each MS medium was made by adding 1 bag of MS salt mix (Nihon pharmaceutical CO., LTD.) into the proper volume of milli-Q (Merck) water ...
-
bioRxiv - Biochemistry 2022Quote: ... or 300 mM (octamer-mix + APLFAD) ammonium acetate at pH 7.5 using 3 kDa MWCO Amicon Ultra Centrifugal Filter Units (Merck Millipore). After buffer exchange the volume of each sample was ∼40 µL ...
-
bioRxiv - Neuroscience 2022Quote: ... sections were mounted on slides and covered with an anti-fading medium using a mix solution 1:10 Propyl-gallate:Mowiol (P3130, SIGMA-Aldrich, Madrid, Spain; 475904, MERCK-Millipore ...
-
bioRxiv - Plant Biology 2022Quote: Fungal elicitor mix was prepared as published previously (24, 38) and separated via enrichment using Discovery DSC-C18 (2 g) columns (Merck) with a H2O/MeOH gradient from 100 % to 0 % H2O in 10 % steps ...
-
bioRxiv - Developmental Biology 2023Quote: ... The reverse transcription reaction to obtain the cDNA was performed with the MystiCq® microRNA cDNA Synthesis Mix kit (Merck) and amplified by PCR using the following primers (miR-34-5p Fw ...
-
bioRxiv - Physiology 2023Quote: ... was used to slowly inject 200 μL of the injection mix composed of ∼2×1011 genome copies of AAV diluted in phosphate-buffered saline (P4417, Merck) containing 2.5 mM MgCl2 and 1 mM KCl ...
-
bioRxiv - Physiology 2023Quote: ... using the 5x QPCR Mix EvaGreen (No Rox) (Solis Biodyne, Cat. # 08-25-00001-20, Estonia) and purified water (Merck Millipore ...
-
bioRxiv - Neuroscience 2023Quote: ... FOs were sonicated 2 x 10 sec in ice-cold 1:2 methanol/chloroform solvent containing SPLASH LIPIDOMIX MS standard internal standard mix (Merck). 1:6 H2O was added to the samples before shaking at 1000 rpm ...
-
bioRxiv - Physiology 2023Quote: ... using the 5x QPCR Mix EvaGreen (No Rox) (Solis Biodyne, Cat. # 08-25-00001-20, Estonia) and purified water (Merck Millipore ...
-
bioRxiv - Microbiology 2019Quote: ... The anions were identified and quantified relative to certified commercial standards (Certipur ®Anion multi-element standard I, II, Merck, Germany). For ICP-MS analysis a certified multi-element stock solution (Perkin-Elmer Pure Plus standard ...
-
bioRxiv - Cell Biology 2022Quote: ... the zona pellucida was removed from metaphase I oocytes or metaphase II-arrested eggs by washing cells through Tyrode’s acid solution (Merck, T1788-100ML) droplets covered with mineral oil (52) ...
-
bioRxiv - Microbiology 2020Quote: ... The anions were identified and quantified relative to certified commercial standards (Certipur ®Anion multi-element standard I, II, Merck, Germany). On the other hand ...
-
bioRxiv - Genetics 2022Quote: In vitro differentiated 18 d erythroid cells stained with Hoechst 33342 were resuspended with 150 μl DPBS for analysis with Imagestream X Mark II (Merck Millipore). Well-focused Hoechst-negative single cells were gated for circularity analysis with IDEAS software ...
-
bioRxiv - Cell Biology 2022Quote: ... they were mechanically disrupted by a 21G needle inside a 3 ml DLD dissociation solution (PluriSTEM® Dispase-II Solution, Liberase, #5401119001 DNase I, #10104159001, all from Merck), and incubated for 20-30 min at 37°C ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... resuspended in 1XPBS and transported in eppendorfs to the image-based cytometer Imagestream Mark II Imaging flow cytometer (Merck, Overijse, Belgium). Measurements were done by acquiring approximately 1×105 single cells per sample ...
-
bioRxiv - Molecular Biology 2019Quote: ... Amplified DNAs were purified with a Wizard SV Gel and PCR Clean-Up System and were used for in vitro transcription with Fluorescein RNA labeling Mix (Merck, #11685619910) and T7 (Merck ...
-
bioRxiv - Cell Biology 2021Quote: ... In situ hybridization was performed on 7-μm thick sections using digoxigenin-incorporated cRNA probes synthesized with SP6 and T7 RNA polymerases using the DIG RNA Labeling Mix (Merck 11277073910). Probes were specific for each target mRNA and did not share more than 35% identity between related transcripts (Supplementary file 1) ...
-
bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
-
bioRxiv - Biochemistry 2023Quote: ... This mix was then deposited in sterilized 5 mm diameter plastic rings cut from PCR tubes (#683201, Greiner bio-one, Merck KGaA) on the surface of a chicken embryo chorioallantoic membrane ...
-
bioRxiv - Cell Biology 2023Quote: ... produced by the mix of 50 ml of sodium hypochlorite 10% (Roth, ref. 9062.3) and 2 ml of 37% hydrochloric acid (Merck, ref. 1.00317.1000). Seeds were then aerated for 10 min in a sterile bench to remove the left-over chlorine gas ...
-
bioRxiv - Synthetic Biology 2021Quote: ... and then cells were transferred to a 96-well plate and thereafter challenged with antibiotics at a concentration of 2×MIC either with or without lipid II 51 (10 μM; provided by Prof. Eefjan Breukink)/LPS (100 μg/mL; Merck, L2880-100MG). The absorbance values were measured by using a Varioskan™ LUX multimode microplate reader (Thermo Fisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... Fat pad tissue was minced and dissociated using the gentleMACS dissociator with an enzymatic digestion solution consisting of 1 mg ml−1 collagenase II (Merck Millipore, 234155), 1 mg ml−1 collagenase IV (Merck Millipore ...
-
bioRxiv - Cell Biology 2022Quote: ... were washed three times using Hank’s balanced salt solution (HBSS) supplemented with 1% of penicillin/streptomycin mix (P/S, Sigma Aldrich/Merck, Poznan, Poland). The endometrium was dissociated from myometrium ...
-
bioRxiv - Developmental Biology 2019Quote: ... Sections were also stained with antibodies against the syncytiotrophoblast layer II marker monocarboxylate transporter 4 (MCT4; Merck Millipore AB3314P, used at 1:100) and against E-cadherin (CDH1 ...
-
bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Bioengineering 2023Quote: ... RBM-II was produced by strain HK-II in BE medium (33) and was collected on a membrane filter (0.1 μm, OmniporeFM membrane filter; Merck Millipore, Burlington, MA, USA) using a suction pump ...
-
bioRxiv - Molecular Biology 2021Quote: ... FA were verified and analyzed by gas chromatography-flame ionization detector (Shimadzu QP2010, Zebron™ ZB-FAME column (7FD-G033-05)) using Supelco® 37 component FAME standard mix (Merck, CRM47885). The internal pentadecyclic acid (15:0 ...
-
bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
-
bioRxiv - Microbiology 2020Quote: ... The internal standard was 1 μL of methyl heneicosanoate (10 mg/mL) and Bacterial acid methyl ester (BAME) mix (Merck-Millipore, Burlington, MA, USA) was used to identify the each peak of fatty acids and analytical standards for each fatty acid were used for quantification.