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Citations for Merck :
101 - 150 of 828 citations for Dengue Virus serotype 3 lysate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Protein concentration of each lysate was determined using a BCA assay (Merck, 71285).
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bioRxiv - Microbiology 2021Quote: ... 0.5 ml of phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 ug/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Microbiology 2021Quote: ... 500 μl of the phage lysate was treated with DNaseI (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was loaded overnight onto a 5mL HisTrap HP column (Cytiva, Merck) equilibrated in 20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... Cleared cell lysate was filter with 0.45 µm sterile filter (Millipore Merck, Schwalbach) and loaded onto a 5 ml HiTrap Chelating HP column (GE Healthcare ...
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bioRxiv - Synthetic Biology 2022Quote: The cell lysate was loaded on 10 mL D-mannose-agarose resin (Merck) pre-equilibrated with Buffer A ...
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bioRxiv - Synthetic Biology 2022Quote: ... the cell lysate was loaded on 10 mL D-mannose-agarose resin (Merck) pre-equilibrated with Buffer A ...
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bioRxiv - Cancer Biology 2023Quote: ... protein lysates were prepared using 1X RIPA buffer containing protease (#539131, Merck Millipore) and phosphatase (#524629 ...
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bioRxiv - Immunology 2023Quote: ... Colony-forming units (CFU) were enumerated in tumour lysates on LB agar (Merck) using standard microbiological techniques.
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate was incubated with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Systems Biology 2023Quote: Caspase-9 activity was measured in liver lysate according to manufacturer’s protocol (Merck Caspase-9 Colorimetric Activity Assay Kit APT139 ...
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bioRxiv - Microbiology 2023Quote: ... The remaining 90% of lysate underwent immunoprecipitation with anti-Flag antibody (Merck, F1804) coupled to Protein G Dynabeads (ThermoFisher ...
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bioRxiv - Neuroscience 2021Quote: RNAs with sequences 5’-AAGGAUGGAUGGAG-3’ (healthy) and 5’-AAGCAUGGAUGGAG-3’ (risk) were synthesised by Merck, resuspended in Ultrapure water ...
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bioRxiv - Plant Biology 2021Quote: Arabidopsis 14-3-3 isoforms Epsilon and Lambda were expressed using the pGEX-4T1 vector (Merck), as a translational fusion with glutathione-S-transferase (GST ...
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bioRxiv - Microbiology 2022Quote: ... was applied onto the dried treatment (10mL of virus was concentrated using centrifugation through Amicon® Ultra-15 Centrifugal Filter Units (Merck). After 5 minutes at room temperature 150μl of DMEM (high glucose ...
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bioRxiv - Microbiology 2020Quote: ... 500 μl of the phage lysate was treated with DNAse-I (Merck cat #11284932001) added to a final concentration of 20 μg/ml and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cancer Biology 2020Quote: ... The lysates were separated through SDS-PAGE and transferred to nitrocellulose membranes (Merck Millipore), blocked in 5% milk in TBS with 0.1% Triton X-100 ...
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bioRxiv - Microbiology 2022Quote: ... 500 µl of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 µg/ml and incubated at 37C for 1 hour to remove bacterial DNA ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg chromatin lysates were incubated with 50 µl FLAG M2 beads (Merck A2220) for 2 h at 4°C with rotation ...
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bioRxiv - Developmental Biology 2022Quote: ... the lysate was concentrated on an Amicon Ultra Centrifugal Filter (Merck Millipore; Billerica, MA), 10μl of the concentrated lysate was resolved on a 4-12% NuPAGE Bis-Tris gel and transferred onto a PVDF membrane using the iBlot system (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... The total lysate was exchanged into PBS buffer using centrifugal filter devices (Merck, UFC201024) according to manufacturer protocols.
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bioRxiv - Microbiology 2022Quote: ... 500 µL of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 mg mL−1 and incubated at 37°C for 1 h to remove bacterial DNA ...
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bioRxiv - Biochemistry 2023Quote: ... Resulting crude lysates were then sonicated for 5 min in an ultrasonic bath (Merck) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 ml of phage lysate was treated with DNase-I (Merck catalogue no. 11284932001) added to a final concentration of 20 µg/ml and incubated at 37 °C for 1 h to remove bacterial DNA ...
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bioRxiv - Genetics 2023Quote: ... 500 µl of the phage lysate was treated with DNase-I (Merck cat #11284932001) added to a final concentration of 20 mg ml−1and incubated at 37°C for 1 hour to remove bacterial DNA ...
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bioRxiv - Molecular Biology 2023Quote: ... The cleared lysate was added to cOmplete His-Tag Purification Resin (Merck Millipore/Roche) and incubated at 4°C for 1.5 h ...
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bioRxiv - Cell Biology 2020Quote: ... and 1x phosphatase inhibitor 3 (Merck). Cell debris was removed by pelleting at 5000g for 10mins ...
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bioRxiv - Immunology 2021Quote: ... 3-Methyladenine (3MA, 5mM; Merck Millipore) or Anakinra (500ng/ml ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-mGluR2/3 (Merck Millipore), rabbit anti-CRTAC1 (Merck Millipore) ...
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bioRxiv - Neuroscience 2019Quote: ... 3-octanol (OCT; 1:1000; Merck) and 4-methylcyclohexanol (MCH ...
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bioRxiv - Neuroscience 2022Quote: ... primarily fixed in 3% glutaraldehyde (Merck) in 0.1 M sodium phosphate buffer with pH 7.2 at 4°C for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... and 3% bovine serum albumin (Merck). The azide-alkyne reaction was performed using the Click-iT™ Cell Reaction Buffer Kit (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... or 3% Rabbit Serum (R9133, Merck)) and finally applying the conjugated antibody ...
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bioRxiv - Neuroscience 2023Quote: ... nystatin (3 units/ml) (Merck: N1638) and ascorbic acid (500 μM ...
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bioRxiv - Neuroscience 2023Quote: ... DAPI (Merck; UK 28718-90-3) was included as a final wash stage ...
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bioRxiv - Physiology 2024Quote: ... - 3% horse serum (Merck, ref H1270) PBS solution for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... 3 units/ ml nystatin (Merck: N1638) and 20 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... The eluates 1-3 were mixed and concentrated with Amicon Ultra-15 (Merck Millipore, MWCO 3 K) to protein concentrations of 44–162 µM.
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bioRxiv - Genomics 2023Quote: ... then immersed in an amino-silane solution (3% vol/vol (3-aminopropyl) triethoxysilane (Merck cat no. 440140), 5% vol/vol acetic acid (Sigma ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Molecular Biology 2021Quote: hEnSC were seeded at a confluency of 105 cells/well in 6 well plates and 1.5 ml of concentrated virus added to each well in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... hTERT cells were transduced with 1.5 ml of concentrated pMY-SUZ12-FLAG-IRES-bls virus in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2022Quote: ... The medium containing the virus was collected 48 hours post-transfection and filtered through a 0.45 µm polyethersulfone (PES) filter (Merck Millipore, Billerica, MA).
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The lysate was then mixed with 1 volume of phenol-chloroform-isoamylalcohol (Merck cat #P2069), and centrifuged for at maximal speed in a table top centrifuge at 4°C for 15 min ...
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bioRxiv - Immunology 2019Quote: Protein lysates from cells were obtained by lysis in radioimmunoprecipitation assay buffer (Merck Millipore, #R0278) with protease and phosphatase inhibitors (Roche ...
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bioRxiv - Immunology 2019Quote: ... Cell lysates were separated by SDS-gel electrophoresis and transferred to PVDF membranes (Merck Millipore). Immunoblotting was performed using the following antibodies from Cell signaling ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Pathology 2023Quote: ... 48h after transfection rAAV8 vectors were harvested from cell lysate and treated with Benzonase (Merck) at 100U/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... the lysate was incubated in the presence of BG4 (Merck, Milan, Italy, MABE817, Lot: 3534080). Anti-FLAG antibody (Sigma-Aldrich ...