Labshake search
Citations for Merck :
101 - 150 of 500 citations for Cow Glycerol kinase GK ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2022Quote: ... Clearing was achieved by incubating the organoids in a fructose-glycerol solution (Merck; Thermo Fisher Scientific). Samples were mounted and images recorded using a Zeiss LSM 780 – SP Mai Tai HP DS accessible through the Cell and Tissue Imaging Cluster (CIC ...
-
bioRxiv - Developmental Biology 2023Quote: ... Stained embryos were washed with 0.2% Tween 20 in PBS and mounted in 50% glycerol (Merck) on a glass slide ...
-
bioRxiv - Biochemistry 2019Quote: ... concentrated and buffer exchanged (kinase NMR buffer, see below) by ultrafiltration (Amicon Ultra-0.5 ml, 3 kDa MWCO, Merck) for NMR analysis.
-
bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
-
bioRxiv - Pathology 2023Quote: ... and then placed in a freeze protective solution (dimethyl sulfoxide in 125 mM PB with 20% glycerol, Merck Life Science AS/Sigma Aldrich Norway AS ...
-
Preference of CAMSAP3 for expanded microtubule lattice contributes to stabilization of the minus endbioRxiv - Biophysics 2023Quote: ... pH 7.3) supplemented with 8% glycerol and 0.5 mM DTT using a 30-kDa-cutoff centrifugal filter (UFC503096; Merck). Finally ...
-
bioRxiv - Neuroscience 2023Quote: ... After processing sections were mounted on microscope slides with Tris-glycerol supplemented with 10% Mowiol (Merck Millipore Calbiochem).
-
bioRxiv - Genomics 2023Quote: ... Slide that now contained stained cells was air dried and mounted with 1ml of 85% glycerol (Merck Millipore) and covered with a coverslip (Menzel-Glaser) ...
-
bioRxiv - Neuroscience 2023Quote: ... and then placed in a freeze protective solution (dimethyl sulfoxide in 125 mM PB with 20% glycerol, Merck Life Science AS/Sigma Aldrich Norway AS ...
-
bioRxiv - Pathology 2019Quote: ... BR) and insulin Elisa assay (cat#EZRMI-13K, Merck Millipore, Billerica, MA, EUA) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2023Quote: ... 2020) containing 300 ng GST-tagged MP-GAP fragments and 200 ng recombinant human Aurora A kinase (Merck, 14-511), 1mCi of [gamma-P32]ATP (Hartmann Analytic ...
-
bioRxiv - Molecular Biology 2021Quote: ... Beads were washed 6 times for 10min with 1ml wash buffer (10mM HEPES ph 7.3, 150mM NaCl, MgCl2, 10% glycerol, 1% Empigen BB Detergent (Merck), 1x PI) ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (30 mM HEPES pH 7.5, 400 mM NaCl, 20 mM imidazole, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
-
bioRxiv - Biochemistry 2022Quote: ... Cells were lysed in lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 5% glycerol, 125 U/ml benzonase (Merck), 1 mM MgCl2 and complete protease inhibitor cocktail [Roche] ...
-
bioRxiv - Immunology 2021Quote: ... Slides were counterstained with Gill’s hematoxylin (Klinipath, Duiven, the Netherlands) and mounted in Kaiser’s glycerol gelatin (Merck, Darmstadt, Germany). Staining was evaluated by microscopy (Leica ...
-
bioRxiv - Systems Biology 2022Quote: ... [13C3] glycerol (12.5%) (Purchased from CK Isotopes, 99% purity) and [15N1] NH4Cl (20%) (Purchased from Merck, 98% atom purity) were the carbon and nitrogen isotopically labelled substrates in the media ...
-
bioRxiv - Genetics 2022Quote: ... The tissue sections were then dried for 5 min at 37°C and mounted with 85% glycerol (Merck, 104094) and a coverslip ...
-
bioRxiv - Neuroscience 2023Quote: ... Cells were stained with DAPI before mounting with Mowiol (12 mL of 0.2 M Tris buffer pH8.5, 6 mL distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore).
-
bioRxiv - Neuroscience 2023Quote: ... Coverslips were shortly rinsed in distilled water and mounted using Mowiöl (12 ml of 0.2 M Tris buffer, 6 ml distilled water, 6 g glycerol, 2.4 g Mowiol 4-88, Merck Millipore). Samples were imaged directly or within the next 48h ...
-
bioRxiv - Molecular Biology 2023Quote: ... The formula was build based on a twofold serious dilution of glycerol (>/= 99% purification, Merck, cat # 56-81-5). The glycerol density (microgram glycerol/mg hyphal weight ...
-
bioRxiv - Microbiology 2023Quote: ... each pure culture pellet was re-suspended with corresponding medium supplemented with 20% (v/v) glycerol (Merck, Darmstadt, Germany) and stored at –80°C.
-
bioRxiv - Immunology 2020Quote: ... followed by the addition of the TMB/E ELISA substrate (Merck Millipore, Billerica, MA). Absorbance was measured as described.
-
bioRxiv - Neuroscience 2023Quote: ... IAPP was measured by an enzyme-linked immunosorbent assay (ELISA; Merck Millipore, EZHA-52K).
-
bioRxiv - Bioengineering 2021Quote: ... Sections were mounted in homemade mounting medium (80% Glycerol (PanReac AppliChem, A1123) in TBS containing 0.2 M N-propyl gallate (Merck, 02370), pH 8.5 ...
-
bioRxiv - Cell Biology 2021Quote: ... the coverslips were mounted on microscopy slides with Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Merck Millipore, 81381). The samples were imaged using a Zeiss Axioscope A1 microscope with a 40x objective.
-
bioRxiv - Bioengineering 2019Quote: ... 1 mM EDTA) and glycerol (2.5%, v/v) were mixed and polymerized by the addition of fresh 10% APS solution (Merck Millipore) (final concentration 10% ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 1-oleoyl-2-acetyl-sn-glycerol (OAG) and 4α-phorbol 12,13-didecanoate (4α-PDD) were obtained from Merck (Gillingham, UK). Sphingosine-1-phosphate (S1P ...
-
bioRxiv - Neuroscience 2022Quote: ... Six rats received an infusion of 1.5 μL (n = 4) or 3.0 μL (n = 2) of 10% glycerol (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM EDTA, 10 % (v/v) glycerol, 1% (w/v) digitonin (Carl Roth GmbH, Karlsruhe, Germany) and cOmplete protease inhibitor (Merck). Samples were rotated on a wheel for 1h ...
-
bioRxiv - Microbiology 2024Quote: ... We stored them at -80 °C in phosphate buffered saline (PBS) with 20 % (v/v) glycerol (anhydrous; Merck, Darmstadt, Germany) and cultivated the isolates on Columbia blood agar plates containing 5 % sheep blood (Mast Diagnostica ...
-
bioRxiv - Microbiology 2024Quote: ... 5 mM DTT and 5%(v/v) glycerol by five cycles of filtration through 10 kDa Amicon filters (Merck Millipore), and finally concentrated to 0.5 mg/ml.
-
bioRxiv - Physiology 2020Quote: ... pancreatic glucagon and GLP-1 content were measured using glucagon ELISA (EZGLU-30K, Merck Millipore), or RIA kit (250-tubes GL-32K ...
-
bioRxiv - Microbiology 2022Quote: ... cultures were maintained at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol ...
-
bioRxiv - Neuroscience 2022Quote: ... was either boiled with 4x SDS sample buffer (4% SDS, 40% glycerol, 20% ß-Mercaptoethanol in 250 mM Tris-HCl, Merck) at 95°C ...
-
bioRxiv - Microbiology 2020Quote: ... 150 mM NaCl and 10 % glycerol) using an Amicon® Ultra-4 regenerated cellulose NMWL 10 kDa centrifugal filter unit (Merck). The protein was then stored as 50 μl aliquots at −80°C ...
-
bioRxiv - Microbiology 2020Quote: ... rhizoxinica were grown at 30 °C in MGY M9 medium (10 g/L glycerol, 1.25 g/L yeast extract, M9 salts) or Standard I Nutrient Agar (Merck, Darmstadt, Germany) supplemented with 1% glycerol.
-
bioRxiv - Cancer Biology 2020Quote: ... dipped in MilliQ, and mounted in Mowiol (0.1 M tris-HCl pH 8.5, 25% glycerol, 10% Mowiol (Calbiochem, Merck Millipore, Darmstadt, Germany)) ...
-
bioRxiv - Plant Biology 2021Quote: ... salicis was stored in glycerol stock at −80 °C and reactivated on tryptic soy broth (TSB) plates (Art. No. 100550, Merck KGaA). After 24 h incubation at 22 °C in the dark ...
-
bioRxiv - Microbiology 2020Quote: ... In the first tube, 6 ml of amniotic fluid was dissolved in 3 ml of glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
-
bioRxiv - Plant Biology 2024Quote: ... Pto DC3000 ΔavrPto ΔavrPtoB was cultured in NYG-medium (0.5% [w/v] peptone, 0.3% [w/v] yeast extract, 2% [v/v] glycerol; Merck KGaA, Darmstadt, Germany) with appropriate antibiotics (50 µg/ml rifampicin ...
-
bioRxiv - Microbiology 2023Quote: ... 10% (v/v) glycerol using an Amicon Ultra-4 centrifugal column with a 10 kDa molecular weight cut-off (Merck-Millipore). This process yielded a protein concentrated to ∼10–20 mg/mL ...
-
bioRxiv - Molecular Biology 2022Quote: 200 μg Pol II was hyperphosphorylated by incubation with 280 units of human MAP Kinase 2/Erk2 Protein (Merck, cat. No, 14-550) in a total volume of 50 μl in kinase buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biochemistry 2023Quote: ... The culture was grown for 2 hr at 37°C and stocks were made from the original plate by adding glycerol (Merck & Co., USA) to the final concentration of 15% and stored at -70°C ...
-
bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
-
bioRxiv - Plant Biology 2024Quote: ... cinerea spores from glycerol stocks were diluted to a concentration of 5 x 104 spores/ml in ¼ PDB (Merck KGaA, Darmstadt, Germany) and pre-germinated for 4 hours at room temperature ...
-
bioRxiv - Immunology 2020Quote: ... All ELISAs were developed with 100 µl substrate solution per well containing 0.11 M sodium acetate (Merck), 0.1 mg/mL 3,5,3’,5’-tetramethylbenzidine (TMB ...
-
bioRxiv - Neuroscience 2023Quote: ... Plasma ferritin was assessed in 324 samples via an ELISA (RAB0197-1KT, Merck Life Science UK Ltd). TBI was calculated using the below equation as proposed by Cook et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... followed by the carbon source (glucose crystals or 99 % glycerol) and filtered sterile using a 0.22 μm pore-size Millex®GP PES filter (Merck-Millipore, Carrigtwohill, Ireland). For the cycA tests ...
-
bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and resuspended in 60 μl same with 1 μl lyticase (17 U/ μl in 10 mM KPO4 pH7, 50% glycerol, Merck >2000 U/mg L2524). Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594) ...