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Citations for Merck :
151 - 200 of 4012 citations for Cow Cysteine rich protein 1 CRIP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Wells were again washed three times with PBS and bound alkaline phosphatase quantified using a p-Nitrophenol phosphate substrate solution for ELISA (Merck) and A405 readings.
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed with three rounds of PBS before incubation in 200 µl p-Nitrophenol phosphate substrate solution for ELISA (Merck) for 10 min ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Genomics 2022Quote: ... followed by quenching with final 1 x Glycine solution for 5 min at RT utilizing the Magna Nuclear RIP (Cross-Linked) Nuclear RNA-Binding Protein Immunoprecipitation Kit (Merck millipore, 17-10520). Cross-linked cells were then centrifuged at 800 x g at 4°C and washed three times with ice cold DPBS ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were digested by addition of porcine trypsin (enzyme to protein ratio of 1:100; for 2 x104 microglial samples, 300 ng was used; Merck) and incubated overnight at 37 °C with shaking ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mg of total protein at a 1 mg/ml concentration was incubated with 40 µl anti-FLAG-M2 affinity resin (Merck) (which had been blocked overnight with 1% BSA and 5 µl/ml ssDNA ...
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bioRxiv - Biochemistry 2022Quote: ... lysates were incubated with anti-HA agarose beads (20 μl of beads per 1 mg of proteins) (Merck, Darmstadt, Germany) for 2 h ...
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bioRxiv - Plant Biology 2022Quote: ... 1 mL of filtered protein extract was mixed with 40μL of anti-FLAG M2 magnetic beads (Merck, formerly Sigma-Aldrich) (equilibrated in GTEN + 0.1 % Tween-20 prior to use ...
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bioRxiv - Microbiology 2023Quote: ... binding of equine antibodies was detected by incubation for 1 hour with protein A conjugated to peroxidase (GE) and visualized with chemiluminescence reagent (ECL, Merck). Images were obtained with an Odyssey LI-COR instrument ...
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bioRxiv - Biochemistry 2019Quote: Insulin and amylin concentration in plasma were determinate by ELISA (Insulin, Cat # EZRMI-13K and Amylin, Cat # EZHA-52K, Merck Millipore) following manufacturer manual ...
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence staining was performed using overnight incubation with polyclonal anti-Prosurfactant Protein C (ProSP-C) (Merck/Millipore/Sigma Aldrich, 1:500) followed by staining with polyclonal secondary antibody Goat anti-rabbit Alexa fluor 488 (Invitrogen,1:500) ...
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bioRxiv - Biochemistry 2020Quote: ... epithelium suspension cell line expressing the EBNA-1 protein (female origin) was cultured in EX-CELL(r) 293 Serum-Free Medium (Merck, 14571C) containing 4 mM GlutaMAX supplement (TermoFisher ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were pre-cleared at 4°C for 1 h on a rotating wheel using 30 µl Protein-S agarose beads (Merck Millipore) slurry ...
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bioRxiv - Cell Biology 2023Quote: ... For fluorescent imaging the 30 µM protein solutions were supplemented with 300 nM (100:1 molar ratio) NTA-Atto 550 (Sigma Aldrich, Merck). Movies were acquired at 37°C with a PerkinElmer UltraVIEW VoX microscope equipped with Yokogawa CSU-X1 spinning disk head and Nikon TiE microscope body with an Apo 60x/1.49 numerical aperture (NA ...
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bioRxiv - Biophysics 2023Quote: ... and the protein of interest was concentrated to 1-2mg/ml in a 50kDa molecular weight cut-off concentrator (Amicon Ultracel, Merck-Millipore). The resulting protein solution was subjected to size exclusion chromatography on a Superose 6 column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... The purified protein sample was concentrated to 40 mg mL-1 using Amicon Ultra-15 3K MWCO Centrifugal Filter Unit (Merck Millipore) and subsequently diluted 1:4 with MilliQ water (yielding 10 mg mL-1 of protein in 1x crystal buffer (10mM Tris ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Genomics 2020Quote: ... The protein solutions were subsequently desalted and protein amount was estimated using Direct Detect cards (Merck Millipore, Darmstadt). All samples were freeze dried before further processing ...
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bioRxiv - Biochemistry 2019Quote: ... The final protein preparations were concentrated to 1 mM using Amicon® Ultra 10 or 3 kDa cut off centrifugal filters (Merck KGaA). Aliquots were frozen in liquid nitrogen and stored in −80 °C.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated for two nights at 4°C with primary antibodies against glial fibrillary acidic protein (GFAP; 1:5000; MAB360; Merck Millipore, USA), ionized calcium binding adaptor molecule 1 (Iba1 ...
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bioRxiv - Neuroscience 2022Quote: ... The pellets were lysed for 1 h using cell protein extraction reagent (CW BioTech, CW0889, China) with protease inhibitor Cocktail set III (1 %, v/v, Merck, 535140, Germany), followed by centrifugation at 500×g for 10 min and the supernatants were collected ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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Heat Shock Factor 1 (HSF1) as a new tethering factor for ESR1 supporting its action in breast cancerbioRxiv - Cancer Biology 2021Quote: ... and eSpCas9-GFP protein (#ECAS9GFPPR, Merck KGaA) were introduced into MCF7 and T47D cells using Viromer® CRISPR (Lipocalyx GmbH ...
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bioRxiv - Evolutionary Biology 2020Quote: ... protein G-magnetic beads (Merck Millipore, LSKMAGG10) were used instead ...
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bioRxiv - Systems Biology 2022Quote: ... proteins were loaded to a filter (Merck-Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Neuroscience 2019Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Cell Biology 2021Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Neuroscience 2021Quote: ... using myelin basic protein primary antibody (MERCK-Millipore ...
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bioRxiv - Biochemistry 2021Quote: ... MWCO 10 kDA protein concentrator (Merck Millipore) to the final concentration of 9-10 mg/ml.
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were transferred onto PVDF membranes (Merck). The membranes were blocked with 5% defatted milk before blotted with primary antibodies and secondary antibodies [anti-mouse IgG-HRP (at 1:2,000 dilution) ...
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bioRxiv - Immunology 2019Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Biophysics 2021Quote: ... proteins were transferred to nitrocellulose membranes (Merck). Nitrocellulose membranes were blocked with 5% (w/v ...
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bioRxiv - Plant Biology 2021Quote: ... separated proteins were transferred to PVDF (Merck) membranes for immunoblotting ...
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bioRxiv - Immunology 2020Quote: Antibody to Ubiquitinated proteins (FK2) from Merck-Millipore was used for the immunoprecipitation assay.
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bioRxiv - Immunology 2019Quote: ... in combination with cas9 nuclease protein (Merck) was used for gene editing ...
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bioRxiv - Molecular Biology 2021Quote: ... protein lysates were reduced with dithiothreitol (Merck) and cysteine residues were alkylated with iodoacetamide (Merck) ...
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bioRxiv - Plant Biology 2022Quote: ... proteins were transferred on a nitrocellulose (Merck) membrane by wet transfer at 15 V for 6h at 4°C and blocked with 2% skimmed milk at room temperature for 2 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... proteins were transferred onto PVDF membranes (Merck) and blocked using 5% non-fat dried milk in PBS-Tween 20 ...
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bioRxiv - Pathology 2023Quote: ... anti-surfactant protein C (SFTPC, AB3786, Merck), homemade anti-α-smooth muscle actin (clone 1A4 ...
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bioRxiv - Cell Biology 2023Quote: ... together with Protein-A Sepharose beads (Merck). Beads were washed 3x in 0.1% (w/v ...