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Citations for Merck :
351 - 400 of 1096 citations for Borrelia Miyamotoi GlpQ Protein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... 100 μl of 10 μg/ml recombinant protein G (Merck, Darmstadt, Germany) was added and incubated for 30 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The protein was filtered through a 0.22 μm Millex-GV filter (Merck) to remove potential aggregates ...
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bioRxiv - Cell Biology 2021Quote: Co-IP was performed using Protein G Immunoprecipitation Kit (Merck, Cat # IP50) as per the manufacturer's protocol ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were concentrated using 30K Amicon Ultra Centrifugal Filters (Merck, Darmstadt, Germany), flash-frozen in liquid nitrogen and stored at −80°C.
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were transferred to Immobilon-P PVDF membrane (Merck Millipore, MA, U.S.A). The membranes were incubated for 1h using 5% low-fat milk solution in 1X TBS (50 mM Tris-Cl ...
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were electrotransferred onto an Immobilon-fl polyvinylidene difluoride (PVDF) membrane (Merck) at 30 V for 1 hour followed by100 V for a further hour at 4°C in 1× NuPAGE Transfer Buffer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Following transfer of the proteins onto a PVDF membrane (Merck Milipore, ISEQ00010), the blots were saturated with 5% BSA in Tris-buffered saline (TBS ...
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bioRxiv - Immunology 2022Quote: ... Proteins were transferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore), which were subsequently blocked with 5% blotting grade blocker (cat# 170-6404 ...
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bioRxiv - Biochemistry 2022Quote: ... Selenomethionine-labelled proteins were expressed in Escherichia coli B834(DE3) cells (Merck). All other proteins were expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The resolved proteins were electrotransferred to an Immobilon-P membrane (Merck, IPVH00010). The membrane was probed with specific antibodies listed in Supplementary Table 3 ...
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bioRxiv - Neuroscience 2020Quote: ... Protein lysates were concentrated using Microcon-10kDa Centrifugal Filter Units (Merck Millipore), according to manufacturer’s instructions and protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... The desalted protein was concentrated on an Amicon 10,000 MWCO (Merck, Germany) and filtered through a 0.22 µM membrane.
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bioRxiv - Microbiology 2019Quote: ... Total protein was isolated from whole cell lysates using BugBuster® (Merck). Total protein concentrations were quantified by Pierce BCA quantification kit (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2019Quote: ... Fractions with addition of thrombin protease (5 Units/mg protein, Merck, www.merckmillipore.com) were pooled and dialyzed against dialysis buffer (10 mM Tris ...
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bioRxiv - Cancer Biology 2019Quote: ... and resolved protein was transferred to Immobilon-P PVDF 0.45µm Membrane (Merck). Proteins were detected using primary antibodies to Actin (Santa Cruz ...
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bioRxiv - Cell Biology 2019Quote: ... anti-pro-surfactant-protein-C (rabbit, 1:20,000, Merck, AB3786, pSP-C), anti-rabbit IgG (goat ...
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bioRxiv - Molecular Biology 2021Quote: ... after which the proteins were transferred to Immobilon P membranes (Merck Millipore). After blocking the membrane with 5% skimmed milk in TBS-T buffer solution for 1 h at RT or overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... protein was concentrated using a centrifugation filter (3K, Amicon, Merck, Darmstadt, Germany) up to a final concentration of 5 mg/mL and stored at −80°C.
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bioRxiv - Biophysics 2019Quote: ... Protein complex was concentrated with Amicon® Ultra Centrifugal Filters (Merck Millipore) and further purified on a Superdex 200 10/300 GL size-exclusion column (GE Healthcare ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were concentrated by ultrafiltration using Amicon Ultra centrifugal filters (Merck Millipore) with respective molecular weight cut offs ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a PVDF membrane (Merck Millipore cat. No. IPFL00010) after which the membrane was blocked using Odyssey Blocking buffer (PBS ...
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bioRxiv - Biophysics 2021Quote: ... proteins were separated from salts and concentrated using Amicon Ultra 15 (Merck). Full length DnaK chaperone was purified by Ni2+-NTA column ...
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bioRxiv - Neuroscience 2020Quote: ... Thirty μl of PureProteome™ Protein A/G Mix Magnetic Beads (Merck) was washed in TBST and then incubated with 5 μl anti-HA antibody for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were transferred onto a PVDF membrane (Immobilon-P, Merck Millipore) and the membranes were blocked with TBS containing 0.1% Tween 20 and 5% non-fat dry milk for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... mutant proteins to the cyanogen bromide (CNBr) activated sepharose 4b resin (Merck). Resin was activated by swelling it into 1 mM HCl according to the manufacturer’s instructions and equilibrated with a coupling buffer (20 mM sodium phosphate pH 8 ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were purified with 10 kDa Amicon filters (Merck, Darmstadt, Germany). Protein concentration was determined with Pierce BCA Protein Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2022Quote: ... All protein concentrations were determined using a Direct Detect Infrared Spectrometer (Merck).
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bioRxiv - Neuroscience 2022Quote: ... IgG from whole blood was purified using protein A Sepharose beads (Merck). Serum was diluted 1:2 in PBS ...
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bioRxiv - Cell Biology 2023Quote: ... The proteins were transferred to an Immobilon-PVDF membrane (Merck, Cat.N. IEVH00005) at 100V for 75 minutes in transfer buffer (20% methanol ...
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bioRxiv - Microbiology 2023Quote: ... followed by the transfer of protein onto the PVDF membrane (Merck Millipore) using a wet-tank blotting system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: Immunoprecipitation was performed using Protein-A Sepharose from Staphylococcus aureus (Merck Millipore) and anti-TKS4 antibody (Lányi et al ...
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bioRxiv - Biochemistry 2023Quote: ... The peak protein pool was concentrated by Amicon Ultra-4 (Merck, #UFC801024), loaded onto a 15–35% glycerol gradient in 5 mL of buffer H containing 0.1 M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Immunology 2023Quote: ... Glycans were extracted with a polyvinylidene-fluoride protein-binding membrane (Merck Millipore). Digested glycans were analyzed by HILIC-UPLC as before (51 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... rabbit polyclonal against Cytoskeleton-associated protein 4 (P63; Merck, Germany; cat # HPA001225), and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46 ...
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bioRxiv - Immunology 2023Quote: Carrier proteins (HEL, OVA, HSA, BSA and MSA) were purchased commercially (Merck) and dissolved in 0.1 M NaCO3 (pH = 8 ...
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bioRxiv - Plant Biology 2023Quote: ... Proteins were detected using α-HA 3F10 antibody (Merck, 11867423001, 1:4000), α-Flag M2 antibody (Merck ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μL of protein RIPA lysis buffer (The Merck Group, Darmstadt, Germany) with proteinase (Halt™ Protease ...
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bioRxiv - Bioengineering 2023Quote: ... Wells were then blocked with protein block (3% BSA fraction V (Merck) or 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... protein detection was carried out with Immobilon Crescendo Western HRP substrate (Merck) using ChemiDoc™ MP System and images were analyzed using Image Lab™ Software (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... and the protein digests were washed twice with water saturated ethylacetate (Merck). Desalting of the proteolytic peptide sample was performed on a C18 solid phase extraction column (‘home-made ZipTip’ from a 15G punch from Empore™ octadecyl C18 47 mm extraction disks (3M ...
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bioRxiv - Cell Biology 2023Quote: ... lysates with equal protein concentration were incubated with Rabbit IgG (Merck, control) and 50 μg of Stwl antibody overnight at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Bound proteins were analysed by mPAGETM 4-20% Bis-Tris gels (Merck) and Coomassie staining ...
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bioRxiv - Bioengineering 2023Quote: ... Proteins were electrotransferred to Immobilon-FL polyvinylidene difluoride (PVDF) membranes (Merck Millipore) and blocked with Intercept Blocking Buffer (LI-COR Biosciences ...
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bioRxiv - Genomics 2023Quote: ... Proteins were visualised using Immobilon Forte HRP substrate (Merck Millipore, MA, USA) and a Fusion FX Chemi Imager (Vilber Lourmat ...
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bioRxiv - Cell Biology 2023Quote: ... Protein levels were detected using chemiluminescent Clarity Western ECL reagent (Merck; GERPN2236) and developed using ImageQuant LAS 4000.
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bioRxiv - Molecular Biology 2023Quote: ... Protein aggregates were removed by centrifugation (0.45 um filter plate, Merck, #MSHVN4550). From the soluble fraction the protein concentration was determined (BCA assay ...
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bioRxiv - Genetics 2023Quote: ... Protein lysate was prepared in Radio-immunoprecipitation assay (RIPA) buffer (Merck Millipore) with a protease inhibitor cocktail (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... Following gel-electrophoresis proteins were transferred to a PVDF membrane (Merck, IPVH00010) using a semi-dry transfer cell (Biorad Transblot SD) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Aqueous solutions without protein components were filtered using Millex-GV filters (Merck), Novec HFE-7500 (3M ...