Labshake search
Citations for Merck :
201 - 250 of 5460 citations for 7 CHLORO N N DIETHYL 4 NITRO 2 1 3 BENZOXADIAZOL 5 AMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2021Quote: ... cells were centrifuged (1500 g for 5 min at 4°C) and resuspended in 3 x PCV buffer A + 0.1% NP40 (Merck Life Science). After 10 further min on ice ...
-
bioRxiv - Biochemistry 2020Quote: ... Prepared solutions were mixed at 3:1 ratio with 20% α-cyano-4-hydroxycinnamic acid (Merck) solution in 20% ACN ...
-
bioRxiv - Cell Biology 2022Quote: ... and Control siRNA Luciferase: 5’ CGUACGCGGAAUACUUCGA 3’ (Merck). HeLa cells were transfected on two consecutive days with 20 nM Cav1 siRNAs using Lipofectamine RNAiMAX (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... supplemented with 2 % (vol/ vol) FCS and and 10 mM 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid (HEPES, Merck) and cut into 1 cm pieces ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... Drugs were dissolved in DMSO at the following final concentrations: ATRA 1μM (cat n° R2625, Sigma-Aldrich Merck), SU5402 25μM (cat n° 572630 ...
-
bioRxiv - Physiology 2023Quote: ... Blots were washed 3 x for 7 min with TBS-T (200 mM Tris (Merck), 1.36 mM NaCl (Merck) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 10 and 3 kDa (Amicon Ultra-4, Merck Millipore) to fractionate the proteins according to their size ...
-
bioRxiv - Cell Biology 2023Quote: ... plates were treated with 3-amino-propyltrimethoxysilane (diluted 1:2 with PBS; Sigma-Aldrich/Merck) for 15min at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... 468-1096) was PCR-amplified using primers 5’-tttggtaccgggccctggctgtgcctg-3’ and 5’-tttctcgagtgcggccgcagatcttag-3’ and subcloned into pET32a(+) vector (Merck Biosciences) in frame with 6xHis tag using restriction sites KpnI and XhoI.
-
bioRxiv - Biophysics 2019Quote: ... Fragments (2 mM in DMSO) were injected (2 μL) onto a Purospher STAR RP-18 end-capped column (3 μm, 30 × 4 mm, Merck KGaA). Chromatographic separation was carried out over a 4-min gradient elution (90:10 to 10:90 water:methanol ...
-
bioRxiv - Biophysics 2023Quote: 1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine (DOPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) stored in chloroform were purchased from Merck (Darmstadt, Germany) and stored under argon ...
-
bioRxiv - Cell Biology 2023Quote: ... The blots were developed in a solution of nitroblue tetrazolium chloride (NBT) and 5-brom-4-chlor-3-indoxylphosphate (BCIP; Merck, Darmstadt, Germany) for 5–30 min at RT ...
-
bioRxiv - Bioengineering 2023Quote: ... 30 mL Expi293 cultures were transfected with HER2 expression plasmid and the supernatant harvested 5-7 days later via centrifugation at 300 G for 5 minutes followed by filtration (Steriflip 0.22mm Merck, SCGP00525). HER2 was then purified from supernatant as previously described (Vazquez-Lombardi et al. ...
-
bioRxiv - Microbiology 2023Quote: ... The lower chloroform layer containing the blue pigment pyocyanin was mixed with 0.1 N HCL (20%v/v; Merck). This caused a change of colour from blue to pink ...
-
bioRxiv - Cell Biology 2023Quote: ... and microforged glass capillaries (25 to 30µm inner diameter) filled with mineral oil (ref. n°M5904, Merck/Sigma-Alrich). 13.8nL of cell suspension from confluent T25 flasks (50×106 cells per ml approx. ...
-
bioRxiv - Genetics 2023Quote: ... CHC profiles of approximately 700 females were extracted in 9 mL HPLC-grade n-hexane (Merck, KGaA, Darmstadt, Germany) which was subsequently evaporated under a stream of gaseous carbon dioxide ...
-
bioRxiv - Immunology 2024Quote: A multiplex biometric ELISA-based immunoassay was used according to the manufacturer’s instructions (MERCK cat. n. MTH17MAG-47K-05) using the Bio-Plex 200 instrument (Bio-Rad Laboratories) ...
-
bioRxiv - Microbiology 2024Quote: ... The lower chloroform layer containing the blue pigment pyocyanin was mixed with 0.1 N HCL (20%v/v; Merck). This caused a change of colour from blue to pink ...
-
bioRxiv - Molecular Biology 2024Quote: ... Thorough vortexing and centrifugation at 11,000 rcf for 5 min was repeated and the upper water phase containing DNA was carefully transferred to a microfuge tube with 0.5 mL H20 saturated n-butanol (Merck). Tubes were vortexed and centrifuged at 11,000 rcf for 5 min ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
-
bioRxiv - Cell Biology 2023Quote: ... TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’; obtained from Sigma-Aldrich/Merck) and non-targeting siRNA (Silencer® Select Negative Control No ...
-
bioRxiv - Microbiology 2023Quote: ... We used sterile-filtered 3 % bovine serum albumin (BSA heat shock fraction, pH 7, > 98 %, Merck) in 1 × Pierce PBS buffer (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ... Slides were mounted in CitiFluor™ CFM3 mounting medium (proSciTech, Australia) containing 3 μg/uL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck, Germany) to stain nucleic acids ...
-
bioRxiv - Biochemistry 2022Quote: The matrix 2′,5′-dihydroxyacetophenone (DHAP; Merck) was applied by sublimation and the data were acquired on a modified timsTOF fleX instrument (Bruker Daltonics ...
-
bioRxiv - Bioengineering 2021Quote: ... Sections were mounted in homemade mounting medium (80% Glycerol (PanReac AppliChem, A1123) in TBS containing 0.2 M N-propyl gallate (Merck, 02370), pH 8.5 ...
-
bioRxiv - Molecular Biology 2022Quote: Phosphorylation of Sam68 N-term and C-term were obtained by incubation with CDK1/Cyclin B (Merck, catalogue 14-450) at a Cdk1:Sam68 ratio of 5:1000 at 20 °C ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... The frozen cell pellets were incubated with 25 mM Tris buffer (pH 7.4) containing 0.05% n-dodecyl-β-D-maltoside (Merck, D4641) and 1 mM CaCl2 ...
-
bioRxiv - Biochemistry 2021Quote: ... were constructed using standard genetic engineering techniques with a His6 tag at the N-terminus using the pET28a vector (Novagen, Merck). The recombinant CAHS1 protein ...
-
bioRxiv - Biochemistry 2021Quote: ... varieornatus CAHS1 protein was constructed and cloned as a fusion protein with a hexahistidine (His6) tag at the N-terminus using a pET28a vector (Novagen, Merck). CAHS1-N (Met1-Ser96 ...
-
bioRxiv - Cell Biology 2019Quote: Pooled extracts of additional control pancreas (n=6) and plasma (n=6) of the experimental groups were filtered with a 30 kDa cut-off Microcon filter (Merck Millipore ...
-
bioRxiv - Microbiology 2019Quote: ... then the procedure was repeated under anaerobic conditions (approx. 0.35% oxygen) with 20 mM Trimethylamine N-oxide dehydrate (TMAO) (Merck, 92277) as a terminal electron acceptor ...
-
bioRxiv - Plant Biology 2023Quote: ... the sequence of CBL1 was fused with an N-terminal 2xStrepII tag in the pET-24b vector (Merck, Darmstadt, Germany). Sequences of active CPK5 and CIPK26 were fused with an N-terminal 2xStrepII tag in the pIVEX-1.3WG vector (Biotechrabbit ...
-
bioRxiv - Cell Biology 2023Quote: ... WM983B cells were infected by lentivirus in the presence of 5µg/mL polybrene (ref. n°TR-1003, Merck/Sigma-Aldrich) followed by antibiotic selection (puromycin 1µg/mL ...
-
bioRxiv - Biochemistry 2023Quote: ... The residue was suspended in 10 µL of n-butanol saturated in water and chromatographed on silica 60 plates (20×20 cm, Merck). Plates were developed for 7-10 cm with isopropyl alcohol/ammonia (32%)/water (6:3:1 by volume) ...
-
bioRxiv - Molecular Biology 2024Quote: ... fixed with 2% paraformaldehyde (PFA) in PHEM and washed once for 5 min at (RT) with 3% BSA (bovine serum albumin, Merck-Sigma, Germany) in Tris-buffered saline with 10 mM EGTA and 2 mM MgCl2 (TBSTEM) ...
-
bioRxiv - Microbiology 2022Quote: ... Five duplicate plates were spread individually by six gradient diluted soil suspensions from 10−2 to 10−7 dilution on 1/10 TSA medium (Tryptic Soy Agar, Merck, Darmstadt, Germany) and incubated under both aerobic and anaerobic conditions at 28 °C for two weeks ...
-
bioRxiv - Cancer Biology 2019Quote: ... 72 hrs or 7 days after adding the drugs organoids were fixed with 4% PFA (Merck) and stained with Hoechst (Invitrogen) ...
-
bioRxiv - Immunology 2021Quote: ... either 4′,6-Diamidine-2′-phenylindole dihydrochloride (Merck) or LIVE/DEAD™ Fixable Near-IR stain kit (Invitrogen ...
-
bioRxiv - Bioengineering 2023Quote: ... 4′,6-Diamidine-2′-phenylindole dihydrochloride (10236276001, Merck); Dox-NP (300112 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... pH 7 using repetitive washing and centrifugation with an Amicon 3 kDa MWCO centrifugal filter (Merck Millipore). For the synthesis of ditopic A’-A’ CC ligand ...
-
bioRxiv - Biophysics 2022Quote: ... and were dissolved in a mixture of chloroform / methanol (7:3 vol/vol, both from Merck KGaA) to yield four stock solutions at 1.5 mM lipid concentration ...
-
bioRxiv - Cell Biology 2023Quote: ... 2 × 3 min Neo-Clear (Cat. No. 1.09843, Merck), 2 × 3 min 100% ethanol ...
-
bioRxiv - Biochemistry 2021Quote: ... 5’-GCCCAAAGAATCAGAACAGATGC-3’) or the genomic 18S ribosome gene (mouse 18S forward: 5’-AAACGGCTACCACATCCAAG-3’, mouse 18S reverse: CAATTACAGGGCCTCGAAAG-3’) (Merck KGaA). Primers specific for mtDNA gives rise to a 201bp product ...
-
bioRxiv - Neuroscience 2022Quote: ... cells nuclei were stained with DAPI (4′,6-diamidino-2-fenilindol, 1:10000, Merck, cat#D9542) for 5 minutes at RT and mounted in Lab Vision™ PermaFluor™ Aqueous Mounting Medium (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Merck Millipore) and mounted on slides with Mowiol (Merck Millipore ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... dried tissue samples (in liquid nitrogen) were dissolved in 3:2:1 mixture of HNO3 (Merck, Germany), H2SO4 (Merck ...
-
bioRxiv - Neuroscience 2020Quote: ... A single intravitreal injection of ML240 or EerI to all P23H rats previously anesthetized (ST and LT: Diethyl ether, Merck Millipore ...
-
bioRxiv - Neuroscience 2022Quote: ... was combined with 90μM sygRNA (5’ - GGATTTGGTAATAGCAG AGGGGG 3’) (Merck) at RT for 15 minutes to form an RNP complex ...
-
bioRxiv - Molecular Biology 2023Quote: ... RNA oligonucleotide G4A4 (5’-AAAAAAGGGGAAAAGGGGAAAAGGGGAAAAGGGGAAAAAA-3’) was purchased from Merck. CD analysis of 2,5 µM RNA was carried out in the buffer used for G4-pulldown ...
-
bioRxiv - Neuroscience 2021Quote: ... mouse anti-HA-7 (Merck H9658; RRID:AB_260092; WB 1:20000) mouse anti-T7-tag (Merck 69522 ...