Labshake search
Citations for Merck :
351 - 400 of 557 citations for 7 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... then dialysed against the same stock of ITC buffer overnight at 4°C using 1 kDa Pur-a-lyzer tubes (Merck). Protein and RNA concentrations after dialysis were calculated by A280 and A260 absorbance respectively ...
-
bioRxiv - Physiology 2020Quote: ... blocked in 5% NGS and 0.5% Triton diluted in PBS and incubated over night at 4°C with primary antibody: rabbit anti-NG2 (1/50, AB5320, Merck), goat anti-PDGFRα (1/200 ...
-
bioRxiv - Neuroscience 2020Quote: ... sections were washed in TBS and demi-water before being dried on a heat plate at 37°C before and cleared in xylene and mounted with Entellan (Merck).
-
bioRxiv - Cell Biology 2020Quote: ... pre-cleared extracts (2 mg) were incubated for 4 hr at 4 °C with 2 μg of pan–ADP–ribose binding reagent (MABE1016, Merck) or normal rabbit IgG (2729S ...
-
bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
-
bioRxiv - Systems Biology 2022Quote: ... Protein precipitation was performed by addition of four volumes of ice-cold acetone (−20°C) (Merck Sigma, Cat. No. 650501) or TCA to a final concentration of 20% and proceeded for 1 hour at -20°C or 30 min at 4°C ...
-
bioRxiv - Molecular Biology 2022Quote: Phosphorylation of Sam68 N-term and C-term were obtained by incubation with CDK1/Cyclin B (Merck, catalogue 14-450) at a Cdk1:Sam68 ratio of 5:1000 at 20 °C ...
-
bioRxiv - Biophysics 2022Quote: HEK293T cells were cultured in 10 cm tissue culture treated dishes grown at 37 °C in a 5% CO2 atmosphere in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine ...
-
bioRxiv - Cell Biology 2022Quote: ... Filters were incubated in a cold room (4°C) overnight with primary antibodies diluted in PBS with 0.1% TWEEN® 20 (Merck) solution (1:1000 dilution) ...
-
bioRxiv - Microbiology 2021Quote: ... protoplasts were overlaid with ½-strength PDA cooled-down to ~50°C and supplemented with 50 mg/ml hygromycin B (Merck). Transformants appearing on the PDA surface ...
-
bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
-
bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
-
bioRxiv - Microbiology 2021Quote: ... The entire 20 l volume was then filtered through a GF/C filter (Whatman, Maidstone, United Kingdom) and 0.45 µm PVDF filter (Durapore, Merck Millipore) to eliminate cells and cellular debris.
-
bioRxiv - Microbiology 2022Quote: ... “treated” cultures were grown with the addition of 0.25 µg ml-1 mitomycin C (MMC) (Merck Life Sciences UK Ltd). Mycelial pellets from untreated and treated cultures were washed in 1x PBS before lysis and RNA purification using the RNEasy Kit (Qiagen) ...
-
bioRxiv - Microbiology 2022Quote: ... Cecum content was collected from freshly sacrificed mice and stored at -70 °C in anaerobic Brain Heart Infusion (BHI) broth (Merck) containing 20% (v/v ...
-
bioRxiv - Neuroscience 2022Quote: Double staining with Fluro Jade C and anti-SARS-COV2 Nucleocapsid antibody were performed by following the protocol from the Fluro Jade C supplier (AG325, Merck) with some minor modifications ...
-
bioRxiv - Biochemistry 2019Quote: ... Nuclei were pelleted by centrifugation at 3000 rpm for 5 min at 4°C and were resuspended in hypotonic buffer containing 1U/µl of benzonase (Merck). Extracts were incubated on ice for 30 min ...
-
bioRxiv - Molecular Biology 2019Quote: ... and CM was concentrated to 1 ml at 4 °C using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
-
bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore ...
-
bioRxiv - Microbiology 2020Quote: ... Samples were stored at 4°C overnight before desilicification with 4% suprapure hydrofluoric acid (Merck; incubation of approximately 5 hours). Afterwards ...
-
bioRxiv - Biochemistry 2019Quote: ... A single colony of freshly transformed cells was cultured at 37 °C in 500 mL 2xYT medium supplemented with 50 µg/mL kanamycin (Merck) and 25 µg/mL chloramphenicol (Sigma Aldrich) ...
-
Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... all proteins were centrifuged (16 000 g, 10 min, 20°C) and filtered (0.1 μm Ultrafree-MC filter, Merck Millipore). Proper performance of the MALS detectors was validated with bovine serum albumin ...
-
bioRxiv - Molecular Biology 2021Quote: ... and the plates were incubated at 33 °C in a 5% CO2 atmosphere in fresh medium with 0.25% Hoechst (Merck-Millipore) to stain the DNA ...
-
bioRxiv - Immunology 2021Quote: Culturing medium was changed 24 h post-transfection and vector supernatants were collected 48 h after transfection and processed by centrifugation at 1000 g for 10 mins at 4°C to remove cellular debris followed by microfiltration using Millex-HV 0.45 μm syringe filter units (Merck – SLHV033RB). Viral supernatants were either kept on ice for further use or frozen down at −80°C for storage.
-
bioRxiv - Molecular Biology 2022Quote: Conditioned medium was centrifuged at 3,200xg for 10 min at 4°C to remove debris and transferred to 10 kDa spin columns (Amicon Ultra, Merck). Proteins that may influence lactate and pyruvate level were removed from the <10 kDa flow-through containing metabolites by centrifugation at 14,000xg for 25 min at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... The whey supernatants were concentrated by centrifugation at 4,000 x g at 20°C using Amicon 100-kDa centrifugal filter units (Merck Millipore) to a final volume of ∼6 mL ...
-
bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...
-
bioRxiv - Molecular Biology 2022Quote: Cells were centrifuged at 3041 × g for 30 minutes at 4°C and supernatant was applied to a 500 mL Stericup-HV sterile vacuum filtration system (Merck) with a pore size of 0.22 µm ...
-
bioRxiv - Microbiology 2023Quote: ... Clarification was then performed by centrifugation for 1 hour at 12,000g and 4°C and vacuum filtration using 45um nylon filter systems (SteriFlip - Merck Millipore). Prior to purification ...
-
bioRxiv - Biochemistry 2022Quote: ... as described by the manufacturer and dried down in a Speed-Vac concentrator (Thermo-Scientific) resuspended in 20 μl L/C water containing 3% acetonitrile (MeCN) (Merck) and 0.1% FA ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were washed with 2 ml 4 °C PBS for 3 times and afterwards fixed in 1 ml of −20 °C cold methanol (Gradient grade for liquid chromatography, Merck) for 5 minutes at −20 °C ...
-
bioRxiv - Microbiology 2022Quote: ... through six dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with 10 kDa molecular weight cutoff (Merck). For experiments studying the IgG-binding properties of Fc-III and Scr ...
-
bioRxiv - Cell Biology 2022Quote: ... in PBS for ten minutes at 4 °C and then washed in 0.1% Tween20 in PBS (PBST) (Merck, Darmstadt, Germany) and blocked with 5% goat sera (Merck ...
-
bioRxiv - Neuroscience 2023Quote: ... Protein samples were digested sequentially with Lys-C and trypsin on Microcon-10 filters (Merck Millipore, #MRCPRT010 Ultracel YM-10). The mixture was centrifuged at 1000 rcf for 5 min ...
-
bioRxiv - Immunology 2023Quote: ... for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest) and 10% DMSO (Merck) using a cell freezing container (CoolCell LX ...
-
bioRxiv - Immunology 2023Quote: ... isolated cells from WAT were counted using cell counting chambers (one-way Neubauer counting chambers, C-Chip, Merck, Darmstadt, Germany). Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit ...
-
bioRxiv - Neuroscience 2023Quote: ... heated to 37°C with a stock solution of gramicidin A (4 mg/ml - dissolved in dimethyl sulfoxide, DMSO, Merck) to achieve a final concentration of 80 μg/mL gramicidin79 ...
-
bioRxiv - Cell Biology 2023Quote: ... Viral supernatants were harvested after at least 24 h of incubation at 32°C and filtered on a 0.45 µm filter (Merck #SE2M230I04).
-
bioRxiv - Biochemistry 2023Quote: ... membrane using wet transfer for 3 h at 90 V/4 °C in transfer buffer (25 mM Tris; 192 mM glycine, Merck; 20% methanol, Merck). Afterwards ...
-
bioRxiv - Molecular Biology 2023Quote: ... The lysate was supplemented with calcium chloride to a final concentration of 3 mM and chromatin was solubilised for 2 h at 4 °C by digestion with Turbonuclease (T4330, Merck) to a final concentration of 250U/ml ...
-
bioRxiv - Cell Biology 2023Quote: ... An aliquot of 40 µL was incubated (1h at 70°C on a heating block) by addition of 20 µL of the trimethylsilylating (TMSi) reagent (chlortrimethylsilane [Merck KGaA ...
-
bioRxiv - Bioengineering 2023Quote: ... Then these samples were incubated in Alexa Fluor 488 conjugated mouse monoclonal anti-CTSK antibody (1:100) at 4°C overnight (Merck) and then for 1h under agitation at room temperature ...
-
bioRxiv - Microbiology 2023Quote: ... JKD6004 and the JKD6009/JKD6008 (VSSA/VISA) pair were routinely cultured at 37°C on solid or in liquid brain heart infusion (BHI, Merck) or Mueller-Hinton (MH ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryo and skin sections were then incubated with the primary antibodies overnight at 4°C (LHX2, 1:1000, #3030529 Merck, Darmstadt ...
-
bioRxiv - Biochemistry 2023Quote: ... HeLa cells were cultivated at 37°C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
-
bioRxiv - Neuroscience 2024Quote: Tissues were dissected out and homogenized at 4°C in RIPA buffer containing protease inhibitors cocktail (Merck Millipore, Milano, Italy) for 30 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... 250 µl of undiluted chromatin was incubated overnight at 4°C with Dynabeads M-270 Epoxy preincubated with anti-Tyr1P (MABE350, Merck), anti-Ser2P (C15200005-50 ...
-
bioRxiv - Plant Biology 2024Quote: ... digestion of roots was performed at 90°C during 4 h in 400 µL of 65%(w/v) HNO3 (Suprapur, Merck). Mineralized samples were diluted in 0.65% (w/v ...
-
bioRxiv - Immunology 2024Quote: ... They were then incubated for 10 minutes at 37°C with Liberase (250 μg/ml) and DNAse (50μg/ml Merck®). After incubation ...