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Citations for Merck :
251 - 300 of 721 citations for 7 8 dimethylbenzo c acridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... we incubated clean glass cover slips (glued to 8-well LabTek chambers) with 30 µg ml−1 fibronectin (Merck) in 1 x PBS for 2 h at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... IP samples were resolved on 8% or 10% SDS-PAGE gels and transferred onto Immobilon-P membranes (Merck Millipore) using a semidry transfer apparatus ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were transduced with sgRNA lentiviruses at an MOI of 3 with 8 µg ml−1 Polybrene (Merck-Millipore) for 4 hours and then reprogramming was initiated by addition of reprogramming medium ...
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bioRxiv - Cell Biology 2021Quote: ... pellets were resuspended in urea lysis buffer (8 M Urea, 50 mM Tris pH 7.5, and 1:200 freshly added protease inhibitor cocktail (Merck)) ...
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bioRxiv - Microbiology 2023Quote: ... and used to transduce TREx BCBL1-Rta cells in the presence of 8 μg/mL of polybrene (Merck Millipore). Virus supernatant was removed 6 hours post transduction and cells were maintained for 48 hours before puromycin (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2023Quote: ... Protein content of supernatant samples were normalized and analyzed using SDS-PAGE (mPAGE Bis-Tris 8%, Merck, Darmstadt, Germany) revealing specific protein bands in the supernatant while comparing wildtype and the T2SS mutant (xpsxcs ...
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bioRxiv - Cell Biology 2023Quote: ... pH 8) and permeabilized and blocked with 1X TBS supplemented with 1% Triton-X100 (MERCK; Cat #9036-19-5) and 6% FBS (Biological Industries ...
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bioRxiv - Biochemistry 2024Quote: ... Dissolved IntC-UbcH5c-CAET-Ub was transferred to a dialyzer (D-Tube Dialyzer Maxi, MWCO 6– 8 kDa, Merck) in 300 mL of unfolding buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell pellet was then lysed in 5X volumes of Lysis Buffer (300μL) [50mM Tris pH 8 (Merck, T6066), 150mM NaCl (Merck ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were transduced with lentiviral particles in the presence of polybrene (8 g/mL, Cat# TR-1003-G, Merck); after 48 hours of transduction ...
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bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Molecular Biology 2022Quote: ... was placed on the top of a water-cooled glass column (33 × 2.5 cm) filled with a slurry of silica gel 60 (with the addition of 7 % water, 40 – 63 μm, Merck, Darmstadt, Germany, # 1.09385.2500) and n-pentane ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Biophysics 2021Quote: ... The His-tag cleaved Mpro in the flow-through was buffer-exchanged to 20mM Tris pH 8 using Amicon Ultra centrifugal filter (MWCO. 10kDa, Merck) and then loaded onto 5mL Q Sepharose column (HiTrap Q HP ...
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bioRxiv - Molecular Biology 2021Quote: ... Smchd1 deletion was confirmed by immunofluorescence with an in-house made anti-Smchd1 antibody during the immunofluorescence experiments (Mab #8, now available commercially from Merck).
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bioRxiv - Pathology 2020Quote: ... was performed placing the sections in a 800 ml glass container filled with the retrieval solutions (10 mM EDTA in Tris-buffer pH 8, Merck), irradiate in a household microwave oven at full speed for 8 min ...
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bioRxiv - Biochemistry 2020Quote: Protein pellets were dissolved in 50 μl of 8 M Urea and loaded onto a 30 K centrifugal filter unit (Merck). Preparation for MS was done as described in [64] ...
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bioRxiv - Neuroscience 2021Quote: ... Dissected tissue was placed in ice cold lysis buffer (150 mM NaCl, 1% NP-40, 50 mM Tris-HCl pH 8, and cOmplete Mini Protease Inhibitor, Merck) and homogenized with a syringe and 20G needle ...
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bioRxiv - Bioengineering 2022Quote: ... the samples were transferred into 50 mL conical tubes with 20 mL of SM buffer (50 mM Tris HCl, 100 mM NaCl, 8 mM MgSO4, pH 7.5, Merck, Germany) and vortexed for 60 s to remove phage Φ6 particles attached to sample surfaces ...
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bioRxiv - Cell Biology 2020Quote: ... MiRNA in situ hybridization was performed in formaldehyde and carbodiimide (EDC)-fixed TA cryo-sections (0,16M 90 min at RT, #25952-53-8, Merck KGaA). After washes with 0,2% glycine (#G8898 ...
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bioRxiv - Microbiology 2022Quote: ... 1.5×104 conidia of each strain were inoculated in 300 µL of RPMI in the presence of 8 µg/mL voriconazole (European Pharmacopoeia, -EP- Reference Standard, Merck) and incubated for 48 hours at 37 °C with occasional shaking ...
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bioRxiv - Plant Biology 2019Quote: ... Cells were then lysed in lysis buffer (50 mM KHPO4 pH 8, 100 mM NaCl, 10 mM Imidazole, 1X BugBuster (Merck), 25 U/ml Benzonase nuclease ...
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bioRxiv - Genomics 2021Quote: ... serial 10-fold dilutions were prepared from the viral stock and used to transduce mESCs in a 6-well plate (Mock plus 10-2 to 10-6) together with 8 ng/µl polybrene (Merck) in duplicates ...
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bioRxiv - Genetics 2021Quote: ... 5 serial 10-fold dilutions of the viral stock were applied to each well of a 6-well mESC plate (MOCK plus 10−2 to 10−6) for transduction with 8 ng/µl polybrene (Merck). Two replicates were generated for each well ...
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bioRxiv - Cancer Biology 2020Quote: ... Gaithersburg, MD, USA) and 8-μm-pore polycarbonate membranes (Nucleopore, Pleasanton, CA, USA) coated with 10 μg/ml fibronectin (Merck) as described elsewhere (Prevete et al. ...
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bioRxiv - Neuroscience 2022Quote: ... cells were replated onto coverglass (8×104 per well) coated with 50 µg/ml Poly-D-Lysine (PDL, Merck, USA) for imaging experiments.
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bioRxiv - Genetics 2022Quote: ... 4 serial 10-fold dilutions of the viral stock were applied to each well of a 6-well mESC plate (MOCK plus 10−3 to 10−6) for transduction with 8 ng/μl polybrene (Merck). Two replicates were generated for each well ...
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bioRxiv - Molecular Biology 2022Quote: ... LV tissue was solubilized in 50 mM Tris-SDS buffer (pH 6.8) containing 8 µg/mL leupeptin (Merck Life Science) and phosphatase inhibitor cocktail (Merck Life Science) ...
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bioRxiv - Cancer Biology 2022Quote: ... were sonicated in 1 M KOH solution (6592-3705, Daejung) for 30 min and then washed with Milli-Q water (Direct 8, Merck) to remove remaining KOH solution ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Microbiology 2023Quote: ... supernatant containing lentivirus was filter sterilised and combined with TREx BCBL1-Rta cells in 8 μg/ml polybrene (Merck Millipore). After 8 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Clones were trial-screened for positive PCR products by making mirror plates and subjecting one of the plates to cell lysis (50mM Tris-HCl pH 8, 1mM EDTA, 0.5% Tween-20, 50-80 μg/ml Proteinase K Merck-3115801001) at 37°C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... depending on manufacturer-suggested minimum seeding density) with lenti/retroviral supernatant in a 1:1 volumetric ratio and 8 µg/mL hexadimethrine bromide (Merck), before centrifugation at 900g for 30 min and returning to incubation at 37°C with 5% CO2 ...
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Linear ubiquitination at damaged lysosomes induces local NF-κB activation and controls cell survivalbioRxiv - Cell Biology 2023Quote: ... Target cells were transduced with 0.5 mL of viral supernatant in 3 mL of total medium supplemented with 8 µg/mL polybrene (Merck Sigma). 2 to 4 days post transduction ...
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bioRxiv - Cell Biology 2023Quote: For the thin layer chromatography (TCL) an aliquot of lipid extract corresponding to 8 mg of DCW was applied to silica gel TLC plates (Merck) by a Linomat 5 semiautomatic sample applicator (Camag) ...
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bioRxiv - Cell Biology 2024Quote: ... Forty eight hours of post-transfection the viral supernatants were passed through 0.45 μm syringe and the diluted viruses were used to infect cells in growth media supplement with 8 μg/ml of fresh polybrene (Merck) for 8 hr for three consecutive times ...
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bioRxiv - Molecular Biology 2023Quote: ... then the mixture was dialyzed against histone refolding buffer at 4 □ using a dialysis tube (D-TubeTM Dialyzer Medi or Maxi, MWCO 6-8 kDa, Merck). HE buffer (10 mM HEPES ...
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bioRxiv - Microbiology 2022Quote: ... 55 mmol L-1 NaCl (Merck KGaA, pH 8.2; T = −27 °C). The mixture was immediately vortexed for 4 seconds and rapidly filtered through cellulose acetate filters (0.45 μm ...
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bioRxiv - Genetics 2021Quote: ... elegans were maintained at 20°C on NGM plates (peptone, P4963, Merck; agar ...
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bioRxiv - Microbiology 2023Quote: ... before imaging at 30 °C in Y04C microfluidic plates (Millipore Merck, UK) connected to an Onix microfluidic perfusion system (CellASIC Corp. ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with monoclonal anti-(c-Myc Tag) antibody (Merck Millipore) (HeLa extracts) ...
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bioRxiv - Biochemistry 2023Quote: ... carbonic anhydrase (30 kDa) and cytochrome C (12 kDa; all from Merck). The relationship between elution volume and molecular mass determined using linear regression ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were maintained at 28°C with Ex-Cell 420 media (Merck), with shaking at 120 rpm for suspension culture and/or grown as static adherent cells ...
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bioRxiv - Molecular Biology 2021Quote: ... and resuspended in 60 μl PFGE wash buffer containing 1 μl lyticase (17 U/μl 10 mM KPO4 pH 7, 50% glycerol Merck L2524 >2000 U/mg) then incubated for 10 min at 50°C ...
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bioRxiv - Physiology 2020Quote: ... Cows treated with the 7-day CO-Synch + CIDR protocol (n = 769) received administration of 100 μg gonadorelin acetate (GnRH; Fertagyl, Merck Animal Health, Madison, NJ) and insertion of a 1.38 g intravaginal progesterone releasing insert (CIDR® Zoetis ...
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bioRxiv - Microbiology 2020Quote: ... 48 and 72 hours) of exposure to simulated solar radiation was performed by using 2’,7’-Dichlorodihydrofluorescein diacetate (DCFH-DA) (Sigma Aldrich-Merck KGaA, Darmstadtm Germany) solubilized in ethanol ...
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bioRxiv - Cell Biology 2023Quote: ... to validate the gene expression the plasmid (vide-supra) was transfected into HEK293 cells using PEI (Sigma-Aldrich/Merck Cat. No. 49553-93-7) following an optimized version of the standard protocol(Rajendra ...
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Microbiology 2019Quote: C6/36 or Huh7 were seeded in 8-well chambers and confluent monolayers were incubated with 150 or 300 μg/mL of HDL (Merck, USA) in specific growth medium without FBS for 1.5 h ...