Labshake search
Citations for Merck :
451 - 500 of 1074 citations for 7 8 DIHYDROXY 4' METHOXYISOFLAVONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
-
bioRxiv - Molecular Biology 2021Quote: hEnSC were seeded at a confluency of 105 cells/well in 6 well plates and 1.5 ml of concentrated virus added to each well in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
-
bioRxiv - Molecular Biology 2021Quote: ... hTERT cells were transduced with 1.5 ml of concentrated pMY-SUZ12-FLAG-IRES-bls virus in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
-
bioRxiv - Bioengineering 2019Quote: ... 200 μL of folded reaction was purified from excess staples and buffer exchanged into 20 mM Tris-HCl pH 8.0 and 8 mM MgCl2 using a 100kDa MWCO spin concentrator (Amicon, Merck Millipore, Billerica, MA). The concentration was subsequently adjusted to 5 nM ...
-
bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
-
bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
-
bioRxiv - Neuroscience 2022Quote: ... Proteins were fractionated on 8% (w/v) sodium dodecyl sulfate polyacrylamide gels and transferred to Immobilon®-P PVDF Membrane (Merck, IPVH00010). Membranes were incubated in 5% (w/v ...
-
bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
-
bioRxiv - Cell Biology 2023Quote: ... HUVECs were seeded at 5,000-10,000 cells per well in 8-well Lab-Tek® chamber slides (Nunc®, Merck, Dorset, UK) and grown to 60-70% confluence ...
-
bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
-
bioRxiv - Neuroscience 2024Quote: ... Postoperative care included antibiotic and analgesic administration (2.27% enrofloxacin, 5 mg/kg, Bayer, Kiel, Germany and 0.3 mg/ml buprenorphine, 8 µg/kg, Buprex, Merck & Co., Inc., NJ, USA) in a controlled temperature environment (37°C) ...
-
bioRxiv - Cell Biology 2020Quote: ... 1 mM EGTA) and fixated for 20 min with 4% paraformaldehyde (Merck). Then ...
-
bioRxiv - Cell Biology 2020Quote: ... and subsequently washed 4 times in Quencher solution (5 mM Trolox (Merck), 10 mM Na-Ascorbate (Merck)) ...
-
bioRxiv - Immunology 2021Quote: ... Purified ACE2 was concentrated in a 50 kDa 4 mL Amicon (Merck), aliquoted and frozen in liquid nitrogen for storage at −80°C.
-
bioRxiv - Biophysics 2019Quote: ... concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) and stored in 1 x PBS at 4 °C.
-
bioRxiv - Biophysics 2019Quote: ... concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) and stored in 1 x PBS supplemented with 50 % Glycerol at −20 °C ...
-
bioRxiv - Neuroscience 2020Quote: ... Using Amicon Ultra-4 30 kDa centrifugal filter units (Merck, Darmstadt, Germany), we transferred the thiolated antibodies to PBS and determined their concentration using the NanoDrop 2000c as described above ...
-
bioRxiv - Microbiology 2021Quote: ... cells were fixed with a 4% paraformaldehyde solution (Sigma-Aldrich, Merck, UK) for 20 minutes ...
-
bioRxiv - Microbiology 2019Quote: Cells were fixed with a 4% paraformaldehyde solution (Sigma-Aldrich, Merck, UK) for 20 minutes ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were treated for 48 h with 3 μM 4-hydroxytamoxifen (Merck) and then kept in 300 nM until experimentation ...
-
bioRxiv - Microbiology 2022Quote: ... Mice were administered with 4 mg/L of dexamethasone (Merck, Lyon, France) through drinking water ...
-
bioRxiv - Biophysics 2022Quote: Cell media was removed and replaced with 4% paraformaldehyde (PFA, Merck KGaA) diluted in phosphate buffer solution (PBS ...
-
bioRxiv - Cell Biology 2022Quote: ... VACV infected cells were fixed with pre-warmed 4% paraformaldehyde (Merck, #P6148) in PBS for 1 h to ensure the inactivation of the virus ...
-
SMDT1 variants impair EMRE-mediated mitochondrial calcium uptake in patients with muscle involvementbioRxiv - Genetics 2022Quote: ... on a 4-20% precast Trupage gel (#PCG2012-10EA; Sigma-Aldrich/Merck). For Western blotting ...
-
bioRxiv - Neuroscience 2022Quote: ... followed by the same volume of 4% paraformaldehyde (PFA) (1.04005.1000, Merck Millipore) in 0.1 M phosphate buffer (PB ...
-
bioRxiv - Immunology 2022Quote: ... and purified by NiNTA beads (Ni-NTA beads, Merck, cat. 70666-4). IFNmod fused with SUMO was purified by the on-column cleavage using 50 mM Tris pH 8 ...
-
bioRxiv - Neuroscience 2023Quote: ... and stained with 5 µM 4′,6-diamidino-2-phenylindole (DAPI) (Merck) in PBS if required ...
-
bioRxiv - Immunology 2023Quote: ... concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off, Merck) and stored at -20°C in 1x PBS supplemented with 50% glycerol.
-
bioRxiv - Neuroscience 2023Quote: ... The supernatant was concentrated with Amicon Ultra-4 (Merck Millipore, MWCO 3K) to approximately 250 µL and analyzed by SEC using a Superdex 200 Increase 10/300 GL column (Cytiva ...
-
bioRxiv - Molecular Biology 2023Quote: ... separated using mPAGE 4-12% Bis-Tris precast gels (Merck Millipore, MP81G15) in mPAGE MOPS SDS Running Buffer (Merck Millipore ...
-
bioRxiv - Molecular Biology 2023Quote: ... All samples were fixed 1:1 with 4% PFA (Merck, no. P6148) (final concentration 2% PFA ...
-
bioRxiv - Biochemistry 2023Quote: ... The peak protein pool was concentrated by Amicon Ultra-4 (Merck, #UFC801024), loaded onto a 15–35% glycerol gradient in 5 mL of buffer H containing 0.1 M NaCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were fixed with 4% paraformaldehyde and treated with hematoxylin (Merck 105174), 0.1% HCl ...
-
bioRxiv - Cancer Biology 2023Quote: ... Sections were fixed with 4% paraformaldehyde and treated with hematoxylin (Merck 105174), 0.1% HCl ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... rabbit polyclonal against Cytoskeleton-associated protein 4 (P63; Merck, Germany; cat # HPA001225), and sheep polyclonal against Trans-Golgi network integral membrane protein 2 (TGN46 ...
-
bioRxiv - Biochemistry 2023Quote: ... Bound proteins were analysed by mPAGETM 4-20% Bis-Tris gels (Merck) and Coomassie staining ...
-
bioRxiv - Cell Biology 2023Quote: ... Peak fractions were collected and concentrated using an Amicon Ultra 4 (Merck). Proteins were analyzed by SDS-PAGE followed by CBB staining (Fig ...
-
bioRxiv - Neuroscience 2024Quote: Cells and neurons were fixed with pre-warmed 4% PFA (#1040051000, Merck) PBS for 15 minutes ...
-
bioRxiv - Biochemistry 2024Quote: ... Peak fractions were collected and concentrated using an Amicon Ultra 4 (Merck). Proteins were analyzed by SDS-PAGE using TGX Stain-Free gel (Bio-Rad) ...
-
bioRxiv - Immunology 2024Quote: ... Vero cells were fixed with 4% paraformaldehyde (PFA) (Sigma-Aldrich; Merck KGaA) for 10 min and stained with 0.1% crystal violet (Sigma-Aldrich ...
-
bioRxiv - Cancer Biology 2024Quote: ... the cells were fixed with 4% paraformaldehyde (PFA) (Sigma-Aldrich; Merck KGaA) for 10 min and stained with 0.1% crystal violet (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2020Quote: ... The Legionella pneumophila DrrA 8-533aa (referred to as DrrA)-encoding DNA was cloned into a modified pET19 vector (Merck Millipore, Burlington, Massachusetts) as previously described (Müller et al. ...
-
bioRxiv - Pathology 2020Quote: ... sections were treated with Weigert’s Iron Hematoxylin for 8 min to stain nuclei and Direct Red 80 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 1 hour at room temperature to visualize collagen before washing with 0.5% glacial acid ...
-
bioRxiv - Neuroscience 2023Quote: ... Whole purified His-MBP-ApoE4M was concentrated to a final concentration of ∼ 8 mg/ml using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). The crystallization was performed in Easy-Xtal 15-well crystallization plates by a hanging drop vapour diffusion ...
-
bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
-
Targeted rescue of synaptic plasticity improves cognitive decline after severe systemic inflammationbioRxiv - Neuroscience 2021Quote: ... Virus was then concentrated using Amicon Ultra-4 centrifugal filter units (Merck-Millipore) and the solution was filtered with a 0.2 µm Acrodisc syringe filter (Sigma-Aldrich).
-
bioRxiv - Developmental Biology 2022Quote: ... Heads including both eyes were fixed in 4% w/v paraformaldehyde (PFA, Merck) in 0.1M phosphate buffer overnight at 4°C and the remaining tissue were kept for genotyping by conventional PCR ...
-
bioRxiv - Microbiology 2021Quote: ... concentrated using an Amicon Ultra-4 10 kDa cut-off spin column (Merck), and stored at -80°C in a storage buffer [100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2020Quote: ... Grids were then stained with 2% (w/v) uranyl acetate pH 4 (Merck) for 1 min ...
-
bioRxiv - Bioengineering 2021Quote: ... Tissues were embedded in 4% low gelling temperature agarose (Merck, Cat#A 0169) and sectioned by vibratome (Leica VT1200 S ...