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Citations for Merck :
151 - 200 of 262 citations for 6 TERT BUTYLQUINOLINE 98.0+% GC T since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... samples were washed 3× five minutes in BSA-T and incubated with an antibody against the FLAG-tag (#F1804 Sigma-Aldrich, now Merck, or #14793 Cell signaling ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were then washed with TBS-T three times for five minutes and exposed to Immobilon Western Chemiluminescent HRP substrate (Merck Millipore) according to product instructions for 30 seconds before being developed ...
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bioRxiv - Immunology 2024Quote: ... Primary human T-cells were isolated from peripheral blood (NHS Blood & Transplant Service, UK) using Ficoll-PaqueTM (Merck Group, Darmstadt, Germany) density gradient centrifugation followed by positive selection using antibody-coated magnetic beads directed against either human CD4 or CD8 antigen (Miltenyi Biotechnology ...
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bioRxiv - Neuroscience 2022Quote: ... cells nuclei were stained with DAPI (4′,6-diamidino-2-fenilindol, 1:10000, Merck, cat#D9542) for 5 minutes at RT and mounted in Lab Vision™ PermaFluor™ Aqueous Mounting Medium (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... and 6) enzymatic reaction to reveal peroxidase with Sigma Fast 3,3’-diaminobenzidine (Merck KGaA, Darmstadt, Germany) used as substrate ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; Merck Millipore) and mounted on slides with Mowiol (Merck Millipore ...
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bioRxiv - Bioengineering 2023Quote: ... and 6 mg/ml 2000 kDa Fluorescein isothiocyanate–Dextran (FITC-Dextran; 46946, Merck, Kenilworth, NJ, USA) (4 ml/kg ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
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bioRxiv - Molecular Biology 2024Quote: 6 mL bone extraction buffer (1 M Trizma base, 0.1 M NaCl, 50 mM TitriplexIII (Merck), 0.5% SDS (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... counterstained with 5µg of 4’,6-diamidino-2-phenylindole (DAPI – 1:1) (Sigma/Merck, D9542-10mg) to eliminate dead cells before running through the flow cytometer ...
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bioRxiv - Microbiology 2020Quote: Quantification of Th1/Th2 cytokines in supernatant of splenocytes was performed using mouse high sensitivity T cell magnetic bead panel assay (MHSTCMAG-70K, Merck, Darmstadt, Germany). 5×105 isolated splenocytes were co-cultured with different stimuli in 200 μL RPMI + 10% FBS ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Bioengineering 2023Quote: ... two thirds of the medium was removed from all wells and T cells were treated with 5 ng/mL polybrene (Merck, Darmstadt, Germany). Lentiviral particles (MOI=3 ...
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bioRxiv - Biophysics 2020Quote: ... Then His-tag containing OpuAC was introduced to the flow cell (in buffer B supplemented with 10 mM of (±)6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; Merck) as a photostabilizer[20] ...
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bioRxiv - Immunology 2022Quote: ... IL-6 and IL-1RA was performed using the Luminex color-coded antibody-immobilized beads from Merck Millipore following the manufacture’s indications ...
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bioRxiv - Cell Biology 2022Quote: Stress assays (wounding or uraemic serum addition) were performed in Corning® 6-well plates (Merck, UK). The HDF ...
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bioRxiv - Biophysics 2019Quote: ... Measurements were done in buffer A supplemented with 1 mM 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox; Merck) and 10 mM Cysteamine (Merck).
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bioRxiv - Cancer Biology 2021Quote: ... cultures were treated with 4 μM of the CDK4/6 inhibitor Palbociclib/PD-0332991 (Merck, PZ-0199) for 8 days ...
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bioRxiv - Immunology 2022Quote: ... Transfections were carried out in 6 well plates with cells at 50–70% confluency using GeneJuice (Merck) with 1 μg of total plasmid DNA per well ...
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bioRxiv - Neuroscience 2022Quote: ... They were recorded in the presence of 10 mM CNQX (6-cyano-7-nitroquinoxaline-2,3-dione, Merck) and 50 mM D-AP5 (D-2-amino-5-phosphonovalerate ...
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bioRxiv - Neuroscience 2023Quote: ... at 1/1000 dilution or Laurdan (#40227, 6-Dodecanoyl-N,N-dimethyl-2-naphthylamine, Merck, Sigma-Aldrich) at 1/500 dilution for 30 min at 37°C in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... gastruloids were incubated with secondary antibodies and 4’,6-diamidino-2-phenylindole (DAPI, 1 µg/mL, Merck) in OWB-SDS at 4°C overnight ...
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bioRxiv - Neuroscience 2023Quote: Trypsinise Ara-C purified SCs using 1 ml of 6% 2 mg ml-1 Trypsin (Merck - 85450C) in Versene (0.02% EDTA (Thermo Fisher - D/0700/53 ...
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bioRxiv - Cancer Biology 2024Quote: ... we plated BC cells (3x105 cells in a 6 well-plate) embedded in collagen type ӏ (Merck) to mimic the breast ECM.
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...
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bioRxiv - Microbiology 2019Quote: ... 6 μl of the reaction was spotted on foil backed silica plate (Silicagel 60, 20 x 20, Merck) and develop in butanol:acetic acid:water 2:1:1 (mobile phase) ...
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bioRxiv - Systems Biology 2020Quote: ... 400 µl were either filled into three wells of a 6-well TPP® tissue culture plate (Merck) supplemented with 0.5 % glucose and incubated under static conditions or used for inoculation of 3 × 10 mL TSB ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
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bioRxiv - Cell Biology 2022Quote: ... then brought to 10 ml with ddH2O) and ECL2 solution (6 µl 30% (v/v) H2O2 (Merck, #H1009) in 10 ml with ddH2O ...
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bioRxiv - Neuroscience 2022Quote: 6-7 dpf zebrafish larvæ were deeply anesthetized using 0.2% Ethyl3-aminobenzoate methanesulfonate (MS222; Merck KGaA, Darmstadt, Germany) diluted in EM ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested 6 hours post transfection through the addition of 1 mL TRI reagent (Sigma-Aldrich, Merck) directly to the monolayer ...
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bioRxiv - Cell Biology 2023Quote: ... beta-galactosidase solution was prepared containing 20 mM citric acid (pH=6) (ref 1.00244.0500, Merck Millipore, Darmstadt, Germany), 5 mM of potassium hexacyano-ferrate (II ...
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bioRxiv - Cell Biology 2020Quote: ... The pellet containing the lentivirus was resuspended in PBS and Jurkat T cells were transduced in complete medium supplemented with 8 μg/mL polybrene (Merck-Millipore, TR-1003-G) using a spinoculation protocol ...
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bioRxiv - Immunology 2023Quote: Concentrations of cytokines in supernatants of T cell cultures were assessed with the MILLIPLEX MAP Human Th17 magnetic bead panel kit (Merck Millipore, Burlington, MA, USA) and the Luminex® 200™ system according to the guidelines of the manufacturer ...
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bioRxiv - Plant Biology 2020Quote: ... and the buffer changed to 6 M urea using Amicon ultra-15 centrifugal filter units with 10,000 MWCO (Merck). The protein concentration was determined spectroscopically at 280 nm on a NanoDrop™ spectrophotometer based on the Lambert-Beer’s law assuming a molecular weight of the CBC-Qprot of 47921 Da and an extinction coefficient of 37,400 M−1 cm−1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the nucleus DNA stain 4′,6-diamidino-2-phenylindole (DAPI) (1μg/ml D9542-10MG, Merck Life Science UK Ltd ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... from an 18500g centrifugation (6 min at +4°C) were run through 3-kDa cut-off centrifugal filters (Amicon Ultra-0.5 ml, Merck) into pre-weighted collection tubes to remove remaining macromolecules ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... mice were fasted for 6 hours prior to being given an IP bolus of 0.75 units/kg insulin (I9278, Merck). Blood was collected at 0 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (6 x 300 µL PBST) and 100 µL of pre-warmed 3,3’,5,5’ tetramethylbenzidine (TMB) solution (Merck-Sigma) was added to each well ...
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bioRxiv - Biochemistry 2024Quote: ... Dissolved IntC-UbcH5c-CAET-Ub was transferred to a dialyzer (D-Tube Dialyzer Maxi, MWCO 6– 8 kDa, Merck) in 300 mL of unfolding buffer ...
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bioRxiv - Cell Biology 2022Quote: ... or 5% (for loading controls) (w/v) non-fat milk powder (NFM; Campina, Amersfoort, Netherlands) in Tris buffer saline with Tween 20 (Merck, Kenilworth, NJ, USA; TBS-T) for 1h at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...