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Citations for Merck :
251 - 300 of 355 citations for 6 Fluoro 8 methoxyquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... 20 μg of protein per sample was loaded and separated on 8-12% SDS–PAGE gels and electro blotted onto polyvinylidenedifluoride (PVDF) membranes (Immobilon-P PVDF membrane 0.45μm; Merck-Millipore, Burlington, USA). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were resuspended in 8 M urea solution in 100 mM Tris-HCl and deposited on top of Microcon®-30K devices (Merck). Samples were centrifuged at 9400 × g ...
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bioRxiv - Bioengineering 2023Quote: ... and 70 samples were buffer-exchanged into 150 mM aqueous ammonium acetate solution (pH 8) using Amicon 50 kDa MWCO centrifugal filters (Merck Millipore). Then ...
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bioRxiv - Neuroscience 2024Quote: Proteins (30 μg) were separated on an 8% SDS-PAGE gel and blotted onto a PVDF membrane (GE10600029 Merck, Darmstadt, Germany). Membranes were blocked for 5 minutes with EveryBlot Blocking Buffer (12010020 Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... 6 μl of the reaction was spotted on foil backed silica plate (Silicagel 60, 20 x 20, Merck) and develop in butanol:acetic acid:water 2:1:1 (mobile phase) ...
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bioRxiv - Systems Biology 2020Quote: ... 400 µl were either filled into three wells of a 6-well TPP® tissue culture plate (Merck) supplemented with 0.5 % glucose and incubated under static conditions or used for inoculation of 3 × 10 mL TSB ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
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bioRxiv - Cell Biology 2022Quote: ... then brought to 10 ml with ddH2O) and ECL2 solution (6 µl 30% (v/v) H2O2 (Merck, #H1009) in 10 ml with ddH2O ...
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bioRxiv - Neuroscience 2022Quote: 6-7 dpf zebrafish larvæ were deeply anesthetized using 0.2% Ethyl3-aminobenzoate methanesulfonate (MS222; Merck KGaA, Darmstadt, Germany) diluted in EM ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested 6 hours post transfection through the addition of 1 mL TRI reagent (Sigma-Aldrich, Merck) directly to the monolayer ...
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bioRxiv - Cell Biology 2023Quote: ... beta-galactosidase solution was prepared containing 20 mM citric acid (pH=6) (ref 1.00244.0500, Merck Millipore, Darmstadt, Germany), 5 mM of potassium hexacyano-ferrate (II ...
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bioRxiv - Cell Biology 2020Quote: ... for 3h at 16°C to remove the 6-His and MBP tag before phase separation in reaction mixtures in buffer C containing 10μM of WT or W1145R TopBP1b6-8-GFP and 2% of PEG4000 (Merck-Sigma-Aldrich, 95904). Reaction mixtures were mixed by gently tapping the Eppendorf ...
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bioRxiv - Molecular Biology 2021Quote: hEnSC were seeded at a confluency of 105 cells/well in 6 well plates and 1.5 ml of concentrated virus added to each well in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... hTERT cells were transduced with 1.5 ml of concentrated pMY-SUZ12-FLAG-IRES-bls virus in the presence of 8 μg/ml polybrene (Merck, TR-1003-G). Cells were then spinoculated at 500 x g for 1 hr at RT and incubated for 12 hrs ...
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bioRxiv - Bioengineering 2019Quote: ... 200 μL of folded reaction was purified from excess staples and buffer exchanged into 20 mM Tris-HCl pH 8.0 and 8 mM MgCl2 using a 100kDa MWCO spin concentrator (Amicon, Merck Millipore, Billerica, MA). The concentration was subsequently adjusted to 5 nM ...
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bioRxiv - Immunology 2019Quote: ... The inhibition of ADAM-17 (a disintegrin and metalloprotease-17) was performed by adding 8 μM TAPI-1 (Merck Millipore, Burlington, MA). TAPI-1 and MK2 IV were added 60 minutes before cytokine stimulation ...
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bioRxiv - Genomics 2021Quote: ... Then the mixture was incubated at 37°C for 16 hours by adding 100µg of Ampicillin (Serva, 69-52-3) and 50µg of sodium azide (Merck Millipore,26628-22-8). After the enzyme digestion ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were fractionated on 8% (w/v) sodium dodecyl sulfate polyacrylamide gels and transferred to Immobilon®-P PVDF Membrane (Merck, IPVH00010). Membranes were incubated in 5% (w/v ...
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bioRxiv - Physiology 2021Quote: ... Membranes blocked with 5% non-fat milk in PBS-T or Tris-Buffered Saline TBS (TBS-T) (RT, 60min) and immunoblotted using the following primary antibodies (4°C, overnight): rabbit anti-pSmad1/5/8 (Merck Millipore, AB3848), rabbit anti-pSmad2 (Cell Signaling ...
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Cell Biology 2023Quote: ... HUVECs were seeded at 5,000-10,000 cells per well in 8-well Lab-Tek® chamber slides (Nunc®, Merck, Dorset, UK) and grown to 60-70% confluence ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 5 μg of plasmid and dsRNA were transfected into BmN4 cells (8 × 105 cells per 10 cm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) every 3 days four times ...
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bioRxiv - Neuroscience 2024Quote: ... Postoperative care included antibiotic and analgesic administration (2.27% enrofloxacin, 5 mg/kg, Bayer, Kiel, Germany and 0.3 mg/ml buprenorphine, 8 µg/kg, Buprex, Merck & Co., Inc., NJ, USA) in a controlled temperature environment (37°C) ...
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bioRxiv - Plant Biology 2020Quote: ... and the buffer changed to 6 M urea using Amicon ultra-15 centrifugal filter units with 10,000 MWCO (Merck). The protein concentration was determined spectroscopically at 280 nm on a NanoDrop™ spectrophotometer based on the Lambert-Beer’s law assuming a molecular weight of the CBC-Qprot of 47921 Da and an extinction coefficient of 37,400 M−1 cm−1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the nucleus DNA stain 4′,6-diamidino-2-phenylindole (DAPI) (1μg/ml D9542-10MG, Merck Life Science UK Ltd ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... from an 18500g centrifugation (6 min at +4°C) were run through 3-kDa cut-off centrifugal filters (Amicon Ultra-0.5 ml, Merck) into pre-weighted collection tubes to remove remaining macromolecules ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... mice were fasted for 6 hours prior to being given an IP bolus of 0.75 units/kg insulin (I9278, Merck). Blood was collected at 0 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (6 x 300 µL PBST) and 100 µL of pre-warmed 3,3’,5,5’ tetramethylbenzidine (TMB) solution (Merck-Sigma) was added to each well ...
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bioRxiv - Biochemistry 2020Quote: ... The Legionella pneumophila DrrA 8-533aa (referred to as DrrA)-encoding DNA was cloned into a modified pET19 vector (Merck Millipore, Burlington, Massachusetts) as previously described (Müller et al. ...
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bioRxiv - Pathology 2020Quote: ... sections were treated with Weigert’s Iron Hematoxylin for 8 min to stain nuclei and Direct Red 80 (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) for 1 hour at room temperature to visualize collagen before washing with 0.5% glacial acid ...
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bioRxiv - Neuroscience 2023Quote: ... Whole purified His-MBP-ApoE4M was concentrated to a final concentration of ∼ 8 mg/ml using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). The crystallization was performed in Easy-Xtal 15-well crystallization plates by a hanging drop vapour diffusion ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 15 cm dishes were seeded and the nuclear translocation of SAMD1 was induced 24 h before extract preparation by adding 200 nM 4-OHT (Merck; 68392-35-8). After collection ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer of the eluted fractions containing 6xHis-HULla was replaced by buffer G (50 mM Tris pH 8, 200 mM NaCl) in Amicon® Ultra – 3 kD Centrifugal Filters (Merck KGaA, Germany) and resulting solution was injected in a heparin column ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: RNA for each biological repeat was extracted from 110 mg of rosette leaves number 5-6 (from at least six plants) with TRI Reagent (Merck) and rounds of phenol-chloroform and chloroform extractions followed by isopropanol precipitation ...
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bioRxiv - Developmental Biology 2019Quote: The multipotent mouse mesenchymal stem cells, C3H10T1/2 cells (Reznikoff et al., 1973) were cultured on 6-wells TPP plastic culture plates (Merck) or 6-wells Uniflex Flexcell plates (FlexCell Int ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 million cells were plated per well in a 6-well plate and cultured for 48h in the presence or absence of 200 nM smoothened agonist (Merck). RNA was extracted and cDNA was generated with Superscript II kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... were assessed by analytical gel filtration using an Akta FPLC UPC-900 equipped with a Superose 6 Increase 10/300 GL column (Merck). Typically ...
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... cells in E8 medium were seeded into a V-shaped 96-well plate (6 000 cells/well) coated with Polyhema (Merck). After 48 hours (Day 0 of the differentiation process ...
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bioRxiv - Microbiology 2021Quote: ... NHS-ester staining: the gel was placed in a 6 well plate and incubated in 594 NHS-ester (Merck, 08741) diluted at 10 μg/mL in 3 ml PBS for 1 hour and 30 minutes at room temperature on a rocking platform ...
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bioRxiv - Immunology 2020Quote: ... The thorax and the skin incision were closed with 6/0 silk sutures (Lorca Marín) and buprenorphine (0.01 mg/kg, Buprex, Merck & Co. Inc) was given for pain relief ...
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bioRxiv - Neuroscience 2022Quote: ... Following counterstaining with 4’,6-diamidino-2-phenylindole (DAPI; Vectashield/Biozol) slices were mounted in Mowiol 4-88 (Merck Chemicals).