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Citations for Merck :
201 - 245 of 245 citations for 6 9 Diaza spiro 4.5 decane 2HCl since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Only treatment naive patients that underwent anti-PD-1 monotherapy with either pembrolizumab (200 mg IV every 3 weeks or 400 mg IV every 6 weeks, Merck) or nivolumab (240 mg IV every 2 weeks or 480 mg IV every 4 weeks ...
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bioRxiv - Molecular Biology 2023Quote: ... then the mixture was dialyzed against histone refolding buffer at 4 □ using a dialysis tube (D-TubeTM Dialyzer Medi or Maxi, MWCO 6-8 kDa, Merck). HE buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
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bioRxiv - Plant Biology 2021Quote: ... Each exudate sample was purified using solid phase extraction (SPE) with C18 Discovery® cartridges (bed wt. 500 mg, volume 6 mL, Merck). Cartridges were activated using 5 mL acetone and washed with 5 mL distilled water ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and host plants was assessed by extracting the compounds following [6] by immersing the samples for 5 min in 5 ml of hexane (99%, SupraSolv, Merck, Germany), followed by removal from hexane with entomological tweezers that were previously cleaned with hexane ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...
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bioRxiv - Pathology 2021Quote: ... The tissues were cut into 6-µm slices and then stained for lipids according to the standard ORO staining protocol (ORO, Merck, Germany). The samples were rinsed with 60% isopropanol and stained with filtered ORO solution (0.5% in isopropanol followed by 60% dilution in distilled water ...
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bioRxiv - Physiology 2021Quote: ... 10 mL of CM or qEV fractions 6-10 diluted in PBS were loaded on 15 mL Amicon Tubes with a cut-off of 100 kDa (Merck, Germany) and spun for 1 hour RT at 5000 xg on a FA-45-6-30 fixed angle rotor ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... The thorax and the skin incision were closed with 6/0 silk sutures (Lab Arago, Spain) and buprenorphine (0.01 mg/kg, Buprex, Merck & Co. Inc) was given for pain relief ...
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bioRxiv - Microbiology 2020Quote: ... In the first tube, 6 ml of amniotic fluid was dissolved in 3 ml of glycerol (≥99 %, G2025, Sigma-Aldrich (Merck), Overijse ...
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bioRxiv - Bioengineering 2022Quote: ... was used to mimic tissue-like scattering conditions and Nigrosin (0.62 v/v% of Nigrosin stock solution [0.5 mg/mL Nigrosin in deionised water, Merck, 8005-03-6]) was added to mimic tissue-like absorption.
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bioRxiv - Cancer Biology 2022Quote: ... Lentiviral transductions were performed in a 6-well plate format (3 × 105 cells/well) using 10 µg/mL polybrene (Merck Millipore). Stably transduced cells were flow-sorted.
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Plant Biology 2022Quote: ... 100 mL of exudate were purified using solid phase extraction (SPE) with C18 Discovery® cartridges (bed wt. 500 mg, volume 6 mL, Merck). Cartridges were activated using 5 mL acetone and washed with 5 mL distilled water ...
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bioRxiv - Cell Biology 2022Quote: ... human very-low-density and low-density lipoproteins (catalog no. 437647-5MG and 6 mg/ml, LP2-2MG, Merck Millipore, respectively) were purchased and stored according to the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2023Quote: ... cells were split into either 6 well-plates (300000 cells per well) coated with 1 mg/ml Poly-L- Lysine (PLL, Merck, P9155) for western blotting ...
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bioRxiv - Cell Biology 2023Quote: ... Cell nuclei were stained with DAPI (4 ’,6-diamidino-2-fenilindol) and slides were mounted using FluorSave™ Reagent (Merck Millipore). The sections were observed and visualized on a Leica DM2500 fluorescent microscope (Leica Microsystems) ...
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bioRxiv - Genomics 2022Quote: ... The NEBuilder reactions were dialyzed against water in a 6 cm Petri dish with a membrane filter MF-Millipore 0.05 µm (Merck, Kenilworth, NJ) for 1 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×105 cells were grown until confluence in 0.2 mg/ml collagen-type I-coated 6 well plates (Merck, Darmstadt, Germany) and incubated in serum-free medium for 24 h with/without Dox treatment ...
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bioRxiv - Plant Biology 2023Quote: ... then 200 µl of each sample was digested for 16 h at 37 °C with α-amyloglucosidase (6 U/ml, Merck), and α-amylase (4 U/ml ...
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bioRxiv - Neuroscience 2024Quote: ... IL-10 and TNF-α were quantified in the FN of NL and at 21dpi aged GFAP-IL6Tg and WT mice (n= 4-6/experimental group) using a Milliplex MAP Mouse High Sensitivity kit (#MHSTCMAG-70K; Merck Millipore), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: DDX5 KO HCT116 cells were transduced in 6-well plates using the lentiviral supernatant diluted in fresh medium supplemented with 4μg/mL of polybrene (Merck, Sigma-Aldrich). The medium was changed after 8 h and the selection with the appropriate antibiotic was initiated after 48 h ...
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bioRxiv - Neuroscience 2020Quote: ... individual COs were transferred to an Eppendorf tube containing CMM medium with 1:100 of virus containing medium and 5µg/ml Polybrene (Merck; TR-1003-6) and incubated over-night ...
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bioRxiv - Immunology 2020Quote: Survival was determined by assessing the fraction of vital cells at defined time points during culture by flow cytometric exclusion of 4’,6-Diamidin-2-phenylindol (DAPI) (Merck, Darmstadt, Germany) and Annexin V-APC (BD Biosciences ...
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bioRxiv - Neuroscience 2020Quote: ... was used as a positive control and incubated simultaneously with MitoSOX Red indicator at a concentration of 1.25 μM and afterwards measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Neuroscience 2020Quote: ... Mitochondria were incubated with 0.2 μM TMRE for 15 minutes and then measured with Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany)
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bioRxiv - Genomics 2021Quote: ... The In-Fusion reactions were dialysed against water in a 6 cm petri-dish with a membrane filter MF-Millipore 0.05 µm (Merck, Kenilworth, New Jersey) for 1 hour ...
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plate was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Physiology 2023Quote: ... the cell culture medium from 6-well plates was collected and either concentrated with Amicon Ultra microconcentrator 10 kDa cut-off (Merck Millipore #UFC501096) or not ...
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bioRxiv - Microbiology 2022Quote: ... Slides were mounted in CitiFluor™ CFM3 mounting medium (proSciTech, Australia) containing 3 μg/uL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck, Germany) to stain nucleic acids ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: OCT-embedded penis sections from humans and rats of 6-8 μm were cut in a cryostat and incubated with or without 4-Hydroxi-TEMPO (4-TEMPOL; Merck, Darmstadt, Germany) 10mM for 30 minutes ...
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bioRxiv - Plant Biology 2023Quote: ... Sections were rinsed 6 times in MTSB and incubated with 1 µg/mL Calcofluor white (Fluorescent Brightener 28 disodium salt solution, Merck, in MTSB). After rinsing ...
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bioRxiv - Molecular Biology 2023Quote: ... a total of 0.4–0.5 μg of plasmid and dsRNAs were transfected into BmN4 cells (4–6 × 104 cells per glass bottom 35 mm dish) with X-tremeGENE HP DNA Transfection Reagent (Merck Millipore/Roche) and cells were fixed 5–6 days later ...
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bioRxiv - Microbiology 2024Quote: ... HEK293T cells transfected with Flag-tagged NSP1 or vector plasmid were treated with the following compounds for 6 h: 10 μM of MG132 (Merck, Cat# 474787), 50 μM of chloroquine (CQ ...
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bioRxiv - Cell Biology 2020Quote: ... Nucleoproteins were separated by electrophoresis on 6/12% sodium dodecyl sulfate (SDS) polyacrylamide gels and blotted onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Darmstadt, Germany). After blocking for 1 h with 5% bovine serum albumin ...
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bioRxiv - Molecular Biology 2021Quote: ... washed and resuspended in PBS and measured by flow cytometry (FACS) using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Fluorescence was excited at 488nm and BODIPY emission was recorded at 530 and 585 nm ...
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bioRxiv - Molecular Biology 2021Quote: ... Apoptotic cell death was detected by annexin-V/propidium iodide staining and subsequent flow cytometry analysis using the Guava Easy Cyte 6-2 L system (Merck Millipore, Schwalbach, Germany). Annexin-V-FITC emission was detected with the green filter at 525/530 nm ...
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bioRxiv - Cell Biology 2021Quote: ... were applied for one hour at room temperature and cells were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI; Merck G8294; 1:3000) before mounting with Fluoromount™.
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bioRxiv - Neuroscience 2020Quote: Different cellular and mitochondrial parameters of the glutamate- or erastin-induced cell death pathways were analyzed using the Guava easyCyte 6–2L flow cytometer (Merck Millipore, Darmstadt, Germany) upon harvesting adherent HT22 cells and following addition of different fluorescent dyes.
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bioRxiv - Biophysics 2022Quote: ... equal amounts of GFP and SNAP-tagged recombinant WT-FUS protein (yielding a total protein concentration of 6 μM) were gently mixed into an aqueous solution of 20 % PEG-35 (Merck KGaA, Darmstadt, Germany) and 500 mM KCl (Merck KGaA ...
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bioRxiv - Developmental Biology 2022Quote: ... MII oocytes were activated for 6 h in Ca-free α-MEM medium containing 10 mM SrCl2 and 5 μM Latrunculin B (cat. no. 428020, Merck Millipore, Darmstadt, Germany). Following activation ...
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bioRxiv - Cell Biology 2023Quote: Differentiation into the three germ layers was performed in suspension culture in Corning® Costar® Ultra-Low Attachment 6-well-plates (Merck, Darmstadt, Germany). Prior to seeding ...
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bioRxiv - Neuroscience 2023Quote: ... 10-15 neurospheres and 4-6 organoids per cell line were rinsed twice with PBS and then lysed in RIPA buffer (Sigma-Aldrich; Merck KGaA, Darmstadt, Germany) supplemented with Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail (Roche ...