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Citations for Merck :
551 - 600 of 817 citations for 4 Methylcaprolactam since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: The cell pellet was fixed in a mixture of 4% formaldehyde and 0.1% glutaraldehyde in 0.1M HEPES (Sigma-Aldrich / Merck) for 1 h at RT ...
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bioRxiv - Microbiology 2024Quote: ... samples maintained at 4°C were eluted through a ZIC-pHILIC column (5 μm, polymeric, 150 by 4.6 mm; SeQuant, Merck) by mobile phase A (20 mM ammonium carbonate ...
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bioRxiv - Genomics 2024Quote: ... media was changed for 500 µL of DMEM +5% FBS +4 μg/mL of polybrene (Merck TR-1003-G). Lentiviruses were diluted to the desired MOI in 500 µL of DMEM +5% FBS and slowly added to each well ...
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bioRxiv - Microbiology 2023Quote: ... 100 ml of the cell suspension at an OD660 of 0.6 was harvested by centrifugation at 5,000×g for 10 min at 4°C and resuspended in 2 ml of BugBuster (Merck), and 50 μg/ml lysozyme and 1 mM phenylmethanesulfonyl fluoride (PMSF ...
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bioRxiv - Microbiology 2020Quote: ... through six consecutive dilution and concentration steps at 4 °C using Amicon Ultra centrifugal filters with a 3 kDa or 10 kDa molecular weight cutoff (Merck). Protein complexes were assembled by mixing the subcomponents at the desired molar ratios ...
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bioRxiv - Biophysics 2021Quote: ... 250 ng of each plasmid encoding VN and VC were transfected using 4 μL of 1 μg/mL PEI (Merck) for each μg of DNA ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... cells were cryopreserved at a concentration of 4*106 cells/ml in 90% N2-S medium and 10% dimethyl sulfoxide (DMSO) (Merck Millipore ...
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bioRxiv - Biophysics 2021Quote: ... fluorescently-labeled and biotinylated H57-scFV were concentrated to 0.2 - 1 mg/mL with 10 kDa Amicon®Ultra-4 centrifugal filters (Merck) and stored in 1x PBS supplemented with 50 % glycerol at -20°C ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... 5×106 cells from treated and untreated conditions were centrifuged for 5 min at 4,500 g and the pellets were resuspended in 4% paraformaldehyde (PFA, Merck, Germany), and incubated for 20 min ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% milk in TBS-T and incubated overnight at 4°C with the primary antibodies anti-tyrosine hydroxylase (1:1,000; AB152; Merck Millipore) or anti-dopa decarboxylase (1:500 ...
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bioRxiv - Biophysics 2019Quote: ... Tag cleavage was carried out overnight at 4 °C on a roller with 10 units of HRV 3C protease (Novagen/Merck chemicals Boulevard Industrial Park ...
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bioRxiv - Biophysics 2019Quote: ... fractions containing monomeric PSCFP2*-AVI-3C-12xHis were concentrated using Amicon Ultra-4 centrifugal filters (10 kDa cut-off, Merck) in the presence of 100 µM Tris(2-carboxyethyl)phosphine (TCEP ...
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bioRxiv - Genetics 2020Quote: ... Bone tissue was cut into 2-mm pieces and placed in PBS buffer containing 4% paraformaldehyde (Schuchardt, Muenchen, Germany) and 1% glutaraldehyde (Merck, electron microscopy grade ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Neuroscience 2020Quote: ... They were cut into 250μm parasagittal slices using a McIlwain tissue chopper and the slices placed on Millicell membrane (3-4 slices per membrane, 2 membranes per animal, 0.4 μm Millicell, Merck Millipore) in 50% BME (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... containing a knockout of DNA Ligase 4) 44 that had been made competent for DNA uptake using the LiCl2-based Yeast transformation kit (YEAST1-1KT; Merck). The transformed cells were plated on minimal synthetic defined (SD ...
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bioRxiv - Biophysics 2020Quote: ... Further concentration and buffer exchange to 20 mM Tris-HCl pH 8.0 were carried out with 10kDa Amicon®Ultra-4 centrifugal filters (MERCK). The mixture of tetramers was then purified by anion exchange chromatography (MonoQ 5/50 GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2021Quote: ... The resulting 5.6-mL fraction was concentrated to 250 μL by ultrafiltration using Amicon Ultra-4 10K centrifugal filters (Merck, USA), then submitted to immunodetection.
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bioRxiv - Neuroscience 2021Quote: ... After washing the sections were incubated overnight at 4°C with anti-NeuN antibody (1:1000, Merck Millipore, Cat. # ABN90P) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... The blots were blocked in PBS/ 0,05%Tween20 containing 5% skim milk and then probed with the following primary antibodies over night at 4°C: mouse anti-P53 (1:100, Merck), rabbit anti-H2AX (1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... The cultured cells were collected by centrifugation (8,000 × g for 10 min at 4 °C) and suspended in BugBuster Protein Extraction Reagent according to the manufacturer’s procedures (Merck Millipore). The supernatant was collected by centrifugation (12,000 × g for 10 min at 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were further separated by size exclusion chromatography (HiLoad 26/600 Superdex 75, Cytiva) and concentrated using Ultra-4 or 15 mL centrifugal filter devices (Amicon, Merck). Correct size and high purity were verified via SDS-PAGE and LC-MS analysis ...
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bioRxiv - Neuroscience 2021Quote: ... then dialysed against the same stock of ITC buffer overnight at 4°C using 1 kDa Pur-a-lyzer tubes (Merck). Protein and RNA concentrations after dialysis were calculated by A280 and A260 absorbance respectively ...
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bioRxiv - Physiology 2020Quote: ... blocked in 5% NGS and 0.5% Triton diluted in PBS and incubated over night at 4°C with primary antibody: rabbit anti-NG2 (1/50, AB5320, Merck), goat anti-PDGFRα (1/200 ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were washed ten times in PBS and ten times in ultrapure water before mounting on slides using Mowiol 4–88 (Merck) containing 200 nM 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Cell Biology 2022Quote: ... Filters were incubated in a cold room (4°C) overnight with primary antibodies diluted in PBS with 0.1% TWEEN® 20 (Merck) solution (1:1000 dilution) ...
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bioRxiv - Microbiology 2021Quote: ... For detection of particle incorporation virus supernatant was further concentrated by centrifugation at 4°C for 2h at 21000xg on 5% Optiprep (Merck), the supernatant was removed and pellet was resuspended and subjected to Western Blot analysis ...
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bioRxiv - Cancer Biology 2021Quote: ... at 4°C and the supernatant was taken for determining protein concentrations using a Direct Detect® Infrared Spectrometer (Merck). Whole lysate was used for subsequent analysis ...
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bioRxiv - Systems Biology 2022Quote: ... The samples are placed in 3k cut-off centrifugal filters (Amicon Ultra −4 Centrifugal filters, Merck Millipore Ltd Cork, Ireland) and spun down at 4500 rpm for 25 minutes using a benchtop centrifuge ...
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bioRxiv - Biochemistry 2019Quote: ... Nuclei were pelleted by centrifugation at 3000 rpm for 5 min at 4°C and were resuspended in hypotonic buffer containing 1U/µl of benzonase (Merck). Extracts were incubated on ice for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... and CM was concentrated to 1 ml at 4 °C using a 10 kDa Centricon Plus-70 centrifugal unit (Merck Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... with 1 % bovine serum albumin (BSA) and incubated overnight at 4 □ in TTBS with 0.1% BSA and the following primary antibodies: mPer1 (#AB2201, Merck Millipore), PER2 (#AB2202 ...
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bioRxiv - Cancer Biology 2019Quote: 3D invasion cultures were washed with phosphate buffered saline pH 7.4 (PBS) once after removing medium and fixed with 4% neutral buffered formaldehyde (Merck, 1.94989.0521) for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... washed with PBS at room temperature before permeabilized for 30 min in PBS containing 0.1% Triton X-100 and 10% normal goat serum and incubated overnight at 4 °C in PBS containing 10% normal goat serum and primary antibodies: anti-Cytokeratin 14 (K14) antibody (SP53; Merck), anti-Neurofilament heavy polypeptide (NF200 ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... and incubated on an orbital shaker for 24h at room temperature + 72h at 4 °C in primary antibody (polyclonal rabbit anti-ERα, Merck-Millipore ...
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bioRxiv - Plant Biology 2019Quote: ... 2.5 mM desthiobiotin [IBA]) and concentrated to a final volume of about 1 mL using Amicon Ultra-4 30 K filters (Merck). Purity of proteins was analyzed by SDS-PAGE using 10 % Mini-PROTEAN® TGX™ Precast Gels (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were fixed with 4% (vol/vol) paraformaldehyde for 10 min at RT and permeabilized with 0.3% Tween 20 (#822184, Merck Millipore). All the primary and secondary antibodies were diluted in blocking buffer consisting of 0.3 M glycine ...
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bioRxiv - Biochemistry 2021Quote: ... Reactions were stopped at each time point using 4 N formic acid and analyzed by PEI-Cellulose-F TLC (Merck) developed in 0.4 M potassium phosphate buffer (pH 3.4) ...
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bioRxiv - Cell Biology 2021Quote: ... Chromatin and its associated proteins were solubilized by incubation with 4- 20 units of Micrococcal nuclease (MNase; Merck Life Sciences) for 10 minutes at 37° C ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 600 ng DNA (per well of a 4-well LabTek dish) using 0.75 μl Genejuice (Merck Millipore) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Culturing medium was changed 24 h post-transfection and vector supernatants were collected 48 h after transfection and processed by centrifugation at 1000 g for 10 mins at 4°C to remove cellular debris followed by microfiltration using Millex-HV 0.45 μm syringe filter units (Merck – SLHV033RB). Viral supernatants were either kept on ice for further use or frozen down at −80°C for storage.
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bioRxiv - Neuroscience 2022Quote: ... Six rats received an infusion of 1.5 μL (n = 4) or 3.0 μL (n = 2) of 10% glycerol (Merck, Darmstadt, Germany) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2022Quote: ... 500ng/ml purified total IgG stimulation was added the next day for 4 hrs followed by an incubation of 30 min with ATP (Merck). Culture supernatants were collected for further cytokine analysis and cells lysates were prepared for qPCR analysis ...
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bioRxiv - Microbiology 2022Quote: ... Coverslips were washed ten times in PBS and ten times in ultrapure water before mounting on slides using Mowiol 4–88 (Merck) containing 200 nM 4′,6-diamidino-2- phenylindole (DAPI) ...
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bioRxiv - Molecular Biology 2022Quote: Conditioned medium was centrifuged at 3,200xg for 10 min at 4°C to remove debris and transferred to 10 kDa spin columns (Amicon Ultra, Merck). Proteins that may influence lactate and pyruvate level were removed from the <10 kDa flow-through containing metabolites by centrifugation at 14,000xg for 25 min at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... gels were washed for around 2-4 h with PBS and incubated in 1 ml 1 μM Alexa Fluor 546 NHS ester (NHS-AF546, #A20002, Merck), overnight at room temperature on a slowly rotating shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections were washed and incubated overnight at 4°C with anti-Digoxigenin-AP antibody (45-11093274910 Merck-SIGMA, 1:1000). Staining was achieved by adding a solution of 4-Nitro blue tetrazolium chloride (NBT ...