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Citations for Merck :
4701 - 4750 of 5346 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1% NP-40) containing protease and phosphatase inhibitors (cOmplete™ and PhosTOP™, Roche/Merck, Dorset, UK), 1 mM sodium orthovanadate and 1 mM phenylmethylsulfonyl fluoride and incubated for 30 min on ice.
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bioRxiv - Microbiology 2023Quote: ... coli RIL strains carrying the appropriate plasmid were grown in 1 l Luria-broth (LB) (Merck Millipore) supplemented with kanamycin (100 μg/ml ...
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bioRxiv - Microbiology 2023Quote: ... Goat Anti-Rabbit IgG HRP conjugate (632131/Merck/Massachusetts, USA, 1:10,000 were procured from Merck (USA). Mouse ß-Actin (3700S/ 1:1000/Cell Signalling technology/Massachusetts ...
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bioRxiv - Cell Biology 2023Quote: ... and fertilized eggs were cultured in EmbryoMax KSOM Medium (1X) w/ 1/2 Amino Acids (Merck Millipore) at 37°C and 5% CO2 ...
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bioRxiv - Cell Biology 2023Quote: ... The chamber was blocked for a period of 12 minutes using 1 mg/ml κcasein (Merck, C0406) in TAB buffer ...
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bioRxiv - Bioengineering 2024Quote: ... in PBS and nuclei were stained using a 1:1000 dilution of DAPI (Merck Life Science NV). Cells were imaged at three to five randomly selected regions using Leica DM-i8 at 20x magnification.
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bioRxiv - Bioengineering 2024Quote: ... THP-1 macrophages on MadSurface were exposed to 10 ng/mL lipopolysaccharide (LPS, Merck Life Science NV) and 10 ng/mL recombinant human interferon-gamma (IFN-γ ...
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bioRxiv - Plant Biology 2024Quote: ... before and after incubation of blots with appropriate secondary antibody (anti-rabbit HRP (Merck #A0545; 1:50,000); anti-mouse HRP (Merck #A9044 ...
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bioRxiv - Molecular Biology 2024Quote: ... The following antibodies were used for the nChIP reactions: rabbit mAbs α-H3K4me3 (1:200; Merck Millipore), α-H3K27me3 (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then washed twice with PBS and detached by treating with 1 mL Accutase™ (Merck) for 30 min at 37°C in 5% CO2 ...
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bioRxiv - Immunology 2024Quote: Control or ISG15-/- fibroblasts were stimulated with 0 or 1,000 IU ml−1 IFN-α2b (Merck IntronA) in normal growth medium for 12 h ...
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bioRxiv - Neuroscience 2024Quote: ... 100 μg/ml penicillin/streptomycin and 1 μM all-trans-retinal (all from Merck KGaA, Darmstadt, DE) and subsequently transfected using the FuGENE HD Transfection Reagent (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... The reaction mixture (1 μl) was spotted onto the glass TLC plate (silica gel 60 F254, Merck) The TLC plates were developed appropriate times with the solution (isopropyl alcohol ...
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bioRxiv - Cancer Biology 2024Quote: ... and secondary antibody anti-rabbit (CST, 1:3000) diluted in PBS containing 4% skim-milk powder (Merck) and 0.2% Tween 20 (Sigma).
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bioRxiv - Biochemistry 2024Quote: ... Supernatant was discarded and pellet was washed once with 1 mL ice-cold 70% ethanol (Merck, 818760.2500) by centrifugation at 13,000 x g for 15 min at 4°C ...
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bioRxiv - Physiology 2019Quote: Fragmented DNA was stained by the terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-digoxigenin nick-end labeling (TUNEL) – method using the ApopTag® Peroxidase In Situ Apoptosis Detection Kit S7100 (Merck Millipore SAS, Molsheim, France) according to the manufactureŕs instructions (Fig 2 B) ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were washed once with DPBS and stained with 1 μg/ml Hoechst 33342 (Merck, Zug, CH) in a Live Cell Imaging Solution (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... in Biotin binding buffer (5 mM Tris-HCl pH 7.5 [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck]) for 1 h at RT ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) supplemented 5 mM benzamidine (Carl Roth), 0.1 mM AEBSF (AppliChem, Darmstadt, Germany) and Benzonase (1:1000, Merck) for 1 h at 4°C on a wheel rotator ...
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bioRxiv - Developmental Biology 2020Quote: ... The gels were subsequently demulsified with 45 µl 1H,1H,2H,2H Perfluoro 1 octanol (PFO) (Merck, #370533) into 200μl of MM+ Ri medium ...
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bioRxiv - Molecular Biology 2020Quote: ... the permeabilized cells were incubated for 1 hour with anti-phospho-S10 Histone H3 (06-570, Merck Millipore) in PBS containing 0.1% Tween-20 (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore).
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bioRxiv - Bioengineering 2019Quote: ... Samples were post-fixed for 1h in a 1% osmium tetroxide solution in 0.1M sodium cacodylate buffer (Merck) and rinsed three times in the same buffer ...
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bioRxiv - Systems Biology 2020Quote: Agarose pads were made by pipetting 1 ml of 1.5 % (wt/vol) low melt agarose (Merck, Darmstadt, Germany) dissolved in PBS onto a 22 mm2 cover glass slide ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed with 4 % paraformaldehyde and stained for doublecortin (guinea pig anti-DCX IgG, 1:400, Merck Millipore followed by Alexa 488 anti-mouse ...
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bioRxiv - Molecular Biology 2019Quote: ... and were hybridized with probes at a concentration of 1 µg/ml in DIG Easy Hyb (Merck, #11603558001). The hybridized probes were detected with anti-Dig AP (Merck ...
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bioRxiv - Evolutionary Biology 2019Quote: All experiments were carried out in casamino acids medium (CAA) containing 5 gl-1 casamino acids (Merck, Switzerland), 1.18 g l−1 K2HPO4*3H2O ...
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bioRxiv - Biochemistry 2020Quote: TSEN complexes were mixed to a final concentration of 1 or 3 μM with 4x SYPRO Orange (Merck) stock in 50 mM HEPES-NaOH ...
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bioRxiv - Microbiology 2021Quote: ... To ensure sterility 1/10 of the inactivated material was incubated in thioglycolate-medium (Merck KGaA, Darmstadt, Germany) for seven days without growth before material was taken out of the BSL3 laboratory.
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bioRxiv - Biochemistry 2020Quote: ... anti-RNA polymerase II subunit B1 (phospho CTD Ser-2) clone 3E10 (Merck, 04-1571-I, 1:5,000), and anti-RNA polymerase II CTD repeat YSPTSPS antibody clone 8WG16 (Abcam ...
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bioRxiv - Plant Biology 2021Quote: ... dried and placed in a ten by ten grid pattern on square culture dishes containing 45 ml of nitrogen replete (+N) (0.5x Murashige and Skoog salts [Caisson Labs], 1% sucrose, 0.5 g/L MES and 0.4% Phytagel [Merck Sigma-Aldrich] ...
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bioRxiv - Systems Biology 2021Quote: ... protease inhibitor EDTA-free (cOmplete™, 1 tablet per 50 mL, cat no. 11873580001, Merck KGaA, Darmstadt, Germany), 1 mM MgCl2 ...
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bioRxiv - Microbiology 2020Quote: ... The following secondary antibodies were used: sheep α-rabbit horseradish peroxidase (HRP) (catalogue number AP510P, 1/10,000; Merck) and goat α-mouse HRP (STAR117P ...
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bioRxiv - Cell Biology 2021Quote: ... 1 mM EGTA and protease inhibitors) on an Amicon 30 kDa cut off concentrator (Merck, Cat# UFC 503096). Protein concentration was determined using a BCA protein assay (Thermo Scientific ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EDTA pH 7.2 with protease inhibitor tablets (cOmplete™, EDTA-free protease inhibitor cocktail, Merck, UK)) and sonicated 30 s on and 30 s off for three rounds using a XL-2020 sonicator (Heat Systems ...
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bioRxiv - Biochemistry 2022Quote: ... the coding sequence was cut out from the pFastBac 1 construct and transferred to pET28a (Merck, Darmstadt, Germany) by means of HindIII and Eco53kI ...
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bioRxiv - Molecular Biology 2022Quote: AKT1/2 phosphorylation was inhibited with InSolution™ Akt Inhibitor VIII (1-10 µM, Merck KGaA, Darmstadt, Germany) in FCS-free medium for 3 hours prior to treatment with HGF (10 ng/µl).
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bioRxiv - Cell Biology 2022Quote: ... For cell cycle distribution the fixed cells were resuspended in Hoechst 33258 (Merck, B2883 1 µg/ml H2O). For nocodazole treatment ...
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bioRxiv - Cell Biology 2022Quote: ... The cells at 13 days and 20 days after electroporation were treated with doxycycline (1 µg/mL, Merck) for 24 hours and subjected to microscopic and flow cytometric analyses ...
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bioRxiv - Biophysics 2022Quote: Purified FHL was concentrated to 1-2 mg/mL using an Amicon centrifugal concentrator (100-kDa cutoff; Merck) and 3 µL of protein solution was applied to freshly glow-discharged C-flat 1.2/1.3 or 2/1 holey carbon grids (Protochips) ...
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bioRxiv - Genetics 2021Quote: YA synchronized wild type and unc-1(vlt10) animals were collected in RIPA 1X buffer (Sigma-Aldrich-Merck) to obtain protein extract ...
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bioRxiv - Biochemistry 2020Quote: ... the water used was type 1 water purified by a Milli-Q® water purification system from Merck Millipore (Billerica ...
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bioRxiv - Cancer Biology 2020Quote: Cells were washed twice with cold PBS and lysed by addition of Tris-buffered saline (TBS)-lysis buffer (TBS [50 mM Tris-HCl pH 7.5, 150 mM NaCl], 0.5% Nonidet P-40, 1× protease inhibitor cocktail [Merck] ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were fixed with 4% paraformaldehyde for 30 min and stained with 1% crystal violet (Merck, Darmstadt, Germany) for 20 min at RT ...
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bioRxiv - Neuroscience 2019Quote: ... cells were fixed using 4% paraformaldehyde and stained with antibodies against ZO-1 (rabbit polyclonal, #617300, Merck-Millipore) and Nestin (mouse monoclonal ...
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bioRxiv - Neuroscience 2019Quote: ... samples were mixed with chilled buffer (50 mM Tris-HCl pH 9, 1% DOC, 50 mM NaF [Merck-Millipore] ...
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bioRxiv - Physiology 2020Quote: ... following which they were incubated with anti-MAP-2 antibody (AB5543, 1:500, Merck Millipore, Burlington, MA, USA) in blocking buffer overnight at 4℃ ...
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bioRxiv - Biochemistry 2021Quote: ... Deionized water was purified using an in-house Synergy Type 1 Ultrapure Water System from Milli-QR (Merck). All solvents were HPLC graded (Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... Methyl viologen dichloride hydrate (paraquat, 98% purity) and Isopropil-β-D-1-tiogalactopiranósido (IPTG) were purchased from Merck.
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bioRxiv - Biochemistry 2021Quote: ... Peak fractions were pooled and concentrated to 10 mg.mL-1 using a 10,000 MWCO centrifugal concentrator (Merck Millipore). Protein was flash-frozen in liquid nitrogen and kept at −80°C until required ...