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Citations for Merck :
401 - 450 of 2042 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... that serves as support for the culture system was prepared seeding human dermal fibroblasts (200,000) re-suspended in a fibrin gel made of fibrinogen (Merck-Millipore), thrombin (Merck-Millipore ...
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bioRxiv - Microbiology 2019Quote: ... balechii culture was collected from each treatment and was filtered using a 0.22-μm nitrocellulose membrane (Merck Millipore, Darmstadt, Germany). The membrane was then transferred into a 2-mL micro-centrifuge tube containing 1 mL DNA lysis buffer (containing 0.1 M EDTA ...
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bioRxiv - Microbiology 2019Quote: A sample (10 mL) was removed from the Gambierdiscus culture and filtered through 3-μm membrane (Merck Millipore, Darmstadt, Germany). One hundred microliters of the filtrate ...
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bioRxiv - Bioengineering 2020Quote: ... 50 μL of culture from underneath the pellicle was removed and placed into the inlet well of a B04A CellASIC ONIX plate (Merck). The plate was placed onto a fluorescence microscope (Nikon Eclipse Ti inverted microscope ...
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bioRxiv - Plant Biology 2020Quote: ... All liquid cultures were incubated for 24 hours at 90 rpm and then filtered through Miracloth (Merck KGaA, MA, USA), by rinsing with SW ...
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bioRxiv - Microbiology 2020Quote: ... cultures were plated on LB (1.6%) agar with 25 μg/mL kanamycin (AppliChem Lot# 1P0000874) or chloramphenicol (Merck Lot# 2430B61), respectively.
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bioRxiv - Biophysics 2022Quote: HEK293T cells were cultured in 10 cm tissue culture treated dishes grown at 37 °C in a 5% CO2 atmosphere in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2021Quote: ... Explants were cultured in 35 mm tissue culture dishes pre-coated with poly-L-lysine (20 µg/ml for 1 hr; Merck) and laminin (20 µg/ml for 1 hr ...
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bioRxiv - Microbiology 2022Quote: ... “treated” cultures were grown with the addition of 0.25 µg ml-1 mitomycin C (MMC) (Merck Life Sciences UK Ltd). Mycelial pellets from untreated and treated cultures were washed in 1x PBS before lysis and RNA purification using the RNEasy Kit (Qiagen) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... normal (24 h) and low pH (24 h) cultures were filtered using a 0.22 μm PVDF filter (Merck Millipore, Germany). These samples were loaded onto an OnGuard column (Dionex OnGuard II Cartridge ...
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bioRxiv - Bioengineering 2019Quote: ... When necessary the Tf and Optiferrin were concentrated from the culture medium by centrifugation through 0.5mL 10KD MWCO Amicon ultra-centrifugal filters according to the manufacturer instructions (Merck Millipore).
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Neuroscience 2020Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Neuroscience 2023Quote: ... culture medium was collected and viral particles were enriched by filter device (Amicon Ultra-15 Centrifuge Filters, Merck, Cat#UFC910008). Titer check was performed on HEK-293T cell culture for every batch of lentiviral preparation.
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Immunology 2023Quote: ... for 24 to 48 hours to arrive at a mature BMDC culture or alternatively frozen to -80°C in 90% FCS (Biowest) and 10% DMSO (Merck) using a cell freezing container (CoolCell LX ...
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bioRxiv - Microbiology 2023Quote: ... Regular testing for mycoplasma contamination was performed to confirm contamination free culture using a PCR-detection kit (Sigma-Aldrich/Merck). The details of cell lines are provided in key resources table ...
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bioRxiv - Microbiology 2023Quote: ... 15 mL of the filtered culture supernatant was first concentrated using Amicon Ultra 15 mL Centrifugal Filters (cut-off 10 kDa, Merck), and the proteins were precipitated out of the solution using trichloroacetic acid (TCA ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Neuroscience 2024Quote: ... Viral particles were harvested from the culture medium 6 days after transfection and were concentrated by precipitation with 8% polyethylene glycol 8000 (Merck) and 500 mM sodium chloride ...
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bioRxiv - Cell Biology 2024Quote: ... 6 x 103 hESCs were seeded on mitotically inactivated MEF feeder in a well of 12-well plates and cultured in hESC culture medium for five days before subjected to alkaline phosphatase staining using Leukocyte Alkaline Phosphatase Kit (Merck) following manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... A 5 mL of the fixed co-culture was filtered on a polycarbonate membrane filter with 0.1 μm pores (VCTP, 25mm, Merck Millipore). Cells trapped on the filter were washed by passing through 500 μL of pure water five times ...
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bioRxiv - Microbiology 2019Quote: ... The cell pellet was washed once with fresh Middlebrook 7H9 liquid media and parallelly a glass coverslip was cleaned once with acetone (Merck Millipore), and once it dried ...
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bioRxiv - Physiology 2019Quote: ... following which they were treated with media containing 20% human serum (fed or fasted) and 1 µM puromycin (Merck Millipore Limited) for a further 4 h ...
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bioRxiv - Biochemistry 2020Quote: ... Next the medium was replaced with a serum-free media supplemented with 2 nM brain-derived neurotrophic factor (BDNF) (Merck Millipore). After 2 days in the BDNF-containing media ...
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bioRxiv - Microbiology 2020Quote: ... The media was further concentrated to ~3 ml with a 100 kDa cut-off centrifugal concentrator filter (Amicon Ultra, Merck Millipore) and layered onto a 20-60% w/v sucrose density gradient in PBS buffer ...
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bioRxiv - Immunology 2021Quote: ... weighed and then dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... method [68] with the following modification: Equal volumes of spent media were concentrated to approximately 50 μL with Microcon - 10 kDa centrifugal filter units (Merck Millipore) at a speed of 14,000 x g ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free supernatant (CFS) was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: Serum-free media from transfected HEK293T was concentrated to about 2 ml with Amicon Ultra-15 Centrifugal Filter 10 kDa MWCO (Merck Millipore) at 3,000 rcf for 15-20 min ...
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bioRxiv - Immunology 2024Quote: ... For analysis of splenic myeloid populations spleens were dissociated using scissors in 1.2 mL of digestion media containing 1 mg/mL collagenase D (Merck Life Sciences) and 0.1 mg/mL DNase I (Merck Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... The sections were subsequently dehydrated through a series of ethyl alcohol solutions and then placed in xylene before being coverslipped with Entellan mounting media (Merck Millipore, USA).
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bioRxiv - Immunology 2021Quote: ... wells washed with 1 X PBS and incubated with 50 µL of incomplete media containing 5 µM Laurdan (Avanti® Polar Lipids, Merck, USA) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... each media sample was concentrated to approximately 100ul using a centrifugal filter unit with a 3kDa cut-off (Merck Millipore, Cork, Ireland), digested with trypsin (2.5μg trypsin ...
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bioRxiv - Developmental Biology 2022Quote: ... 32.8 mM 20:4 and 60 mM 22:6) and pipetted dropwise into N2 media containing 3 mM fatty acid-free BSA (Merck, Cat. #A8806) at 37°C with constant stirring to obtain final concentrations of 3 mM 18:0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Media was concentrated further to a final volume of 1-2 ml using 10 KDa Amicon spin filters (Merck Millipore, MA, USA) by centrifugation at 3,500 g ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Bioengineering 2022Quote: ... – except for those sections using AEC as the visualization system which required an aqueous mounting media (Cat#108562, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Neuroscience 2023Quote: Flies were collected on the day of eclosion and kept in the dark on the fly media containing 0.4 mM all trans-Retinal (Merck, St. Louis, MO). 7-10 days old flies were cold-anesthetized using a temperature-controlled stage and mounted on a plastic plate with a small window using wax ...
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bioRxiv - Neuroscience 2023Quote: ... Flies were raised at 25℃ and collected on the day of eclosion and kept in the dark for 7-10 days on the fly media containing 0.4 mM all trans-Retinal (Merck, St. Louis, MO). The experiments were performed with the “8-well” chamber (16 mm diameter x 10 mm height ...
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bioRxiv - Evolutionary Biology 2019Quote: ... ∼105 log-phase cells from 200 ml mass culture were concentrated on hydrophilic nylon membranes (Nylon-Netzfilter, 10 µm pore size, 47 mm, Merck KGaA), rinsed in mineral water (Volvic® ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... the growth of pure culture of Halomicrobium was obtained in the upper chamber of Millipore® Stericup® filtration system (Merck) (capacity 250 ml ...
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bioRxiv - Bioengineering 2021Quote: ... INS-1 cells in bioprinted grid scaffolds were stained with thiazolyl blue tetrazolium bromide (MTT) after 5 days in culture according to the manufacturer’s protocol (Merck, Darmstadt, Germany). Proliferation was determined using an ATP-based assay with luminescent readout (CellTiter-Glo® 3D Cell Viability Assay ...
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bioRxiv - Neuroscience 2022Quote: ... A sequence of coronal slices (400 μm) was cut at the level of the somatosensory cortex and plated on Millicell-CM organotypic culture membrane inserts (Merck Millipore). Slices were cultured in a medium containing 50% Optimem-1 ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Cell Biology 2022Quote: ... crescentus cultures were grown overnight at 30 °C in 3 ml of 2x PYE49 (Peptone, Merck, #82303; Yeast Extract, Merck, #Y1626) medium under mechanical agitation (200 rpm) ...
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bioRxiv - Developmental Biology 2022Quote: Dissected lung lobes were placed onto a hydrophilic polytetrafluoroethylene organ culture insert with a pore size of 0.4 µm (Merck Millipore, #PICM01250), which was preset in a 35 mm petri dish filled with 800 µL of culture medium ...
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bioRxiv - Bioengineering 2020Quote: All circuits were made on tissue-culture-treated surfaces – 60 or 35 mm circular dishes (Corning, Merck product 430166 or #430165) or rectangular uniwell plates with overall dimensions of 96-well plates (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... Culture supernatants were harvested at 3 days post-transfection and filtered through a 0.45 μm filter (Merck Millipore, Burlington, MA, USA). All proteins were purified with a Talon metal affinity resin (Takara Bio USA ...
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bioRxiv - Molecular Biology 2024Quote: ... Viability analysis was performed by staining parasites in culture medium with 0.01 mg/ml of propidium iodide (P4864, Merck Life Sciences). For cell cycle and PAD1 expression analysis ...