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Citations for Merck :
401 - 450 of 3895 citations for Human Glutamine Fructose 6 Phosphate Transaminase 1 GFPT1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: Human fibrinogen from non-diseased donors (Merck Millipore, #341576-1GM) was reconstituted in sterile 0.9% saline to a stock concentration of 67mg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... and human plasmatic butyrylcholinesterase (hBChE, E.C. 3.1.1.8, purchased from Merck) were determined using the modified Ellman’s method ...
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bioRxiv - Cell Biology 2023Quote: ... the media of the cells was replaced with XF base media supplemented with 2 mM L-Glutamine (Merck Life science), 17.5 mM glucose (Fischer Scientific) ...
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bioRxiv - Immunology 2024Quote: ... L-cystine dihydrochloride, RPMI 1640 medium (with sodium bicarbonate, without methionine, cystine and L-glutamine) and homoharringtonine were purchased from Merck. Fetal calf serum (FCS ...
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bioRxiv - Biochemistry 2024Quote: ... containing labeled or unlabeled glucose/glutamine and quenched by adding 50% ice-cold methanol containing 2 µg/mL cinnamic acid (Merck) as an internal control ...
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bioRxiv - Bioengineering 2021Quote: Extracellular alkaline phosphate (ALP) qualitatively evaluated at D7/14/21 with Fast-Violet B Salt and Naphthol-AS-MX ALP staining (Merck KGaA). Cells fixed with a solution ...
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bioRxiv - Cell Biology 2020Quote: ... the samples containing 20 nmol phosphate were subjected to TLC on an HPTLC plate (Silica gel 60; Merck Millipore, MA, USA) in the solvent system hexane/diethyl ether/acetic acid (80:20:1 ...
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bioRxiv - Neuroscience 2019Quote: ... detached cells were then added to phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA) to a final concentration of 10 μM and dissociated to single cells ...
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bioRxiv - Cell Biology 2021Quote: ... human iPSC cells were detached using Accutase for 5min at 37 °C and followed dissociated into single cells with phosphate-buffered saline (PBS; LGC Biotechnology, USA) containing 10μM ROCK inhibitor (ROCKi, Y27632; Merck Millipore, USA). Cells were centrifuged at 300 rpm for 5 min and the pellet was resuspended in hESC medium (20% knockout serum replacement ...
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bioRxiv - Neuroscience 2022Quote: ... The virus-containing fraction was extracted from the tubes and AAVs were then concentrated in Dulbecco’s Phosphate Buffered Saline (D-PBS) + 5% sucrose using Amicon Ultra-15 (100K) filter units (Merck Millipore UFC910024) at 3220 g ...
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bioRxiv - Biochemistry 2023Quote: ... the samples were dialyzed overnight against 10 mM phosphate buffer (pH 7.5) and filtered with Ultrafree-MC-GV Centrifugal Filters (Durapore-PVDF, 0.22 μm) (Merck KGaA, Darmstadt, Germany). Filtered samples containing 0.15-0.20 mg/ml of protein were loaded into a 1-mm quartz cuvette (Hellma ...
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bioRxiv - Bioengineering 2020Quote: ... and 100 μl dispensed in triplicate into wells of 96-well ELISA plates (Nunc Maxisorp; Merck Ltd., Poole, United Kingdom) preblocked with 1w/v BSA in PBS and precoated with monoclonal anti–IFN-γ (U-Cytech ...
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bioRxiv - Cell Biology 2021Quote: ... collagen-grown cells were collected and incubated with ITGA-6 neutralizing antibody (Merck Millipore #MAB1378 ...
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bioRxiv - Cell Biology 2022Quote: Wound healing assays were performed in Corning® 6-well plates (Merck, UK). HDF and MSC were individually labelled with cell tracking dyes eBioscience™ CFSE (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... The nuclei were stained with 4′,6-diamidin-2-phenylindol (DAPI, Merck, Germany).
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bioRxiv - Neuroscience 2024Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI) (D9542) (all from Merck, Auckland, NZ). See table S2 for antibodies used for immunocytochemistry (ICC).
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bioRxiv - Microbiology 2024Quote: ... phage lysates were purified using Vivaspin 6 centrifugal concentrators (MWCO 1,000,000 kDa; Merck). The lysate was washed several times with SM buffer (100 mM NaCl ...
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bioRxiv - Immunology 2024Quote: ... we included αTIM-3+ αPD-1 and used anti-human RSV-IgG4 as an isotype control antibody (5 μg/mL, 60AGK S228P, Merck & Co., Inc., Rahway, NJ, USA). On day six ...
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bioRxiv - Microbiology 2019Quote: ... DT40 cells (immortalised chicken B cell line [16]) were maintained in Roswell Park Memorial Institute (RPMI) media supplemented with L-glutamine (Sigma-Aldrich, Merck), sodium bicarbonate (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... with 4.5 g/l (high) glucose and L-glutamine supplemented with 10 % fetal bovine serum (volume fraction, Sigma Aldrich/Merck KGaA) without antibiotics at 37 °C and 5 % CO2 in a humidified atmosphere and regularly confirmed to be mycoplasma negative ...
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bioRxiv - Microbiology 2023Quote: ... Culturing and biofilm formation were performed in RPMI-1640 (with l-glutamine and without bicarbonate, pH 7.0, and with MOPS; Merck, Budapest, Hungary). Farnesol (Merck Ltd. ...
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bioRxiv - Genomics 2024Quote: ... in modified RPMI with L-glutamine and without biotin and phenol red (US biological life sciences, R9002-01) supplemented with 200 µM Hypoxyxanthine (Merck, H9377) and 0.5% AlbuMAX TM II (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... The viral particles were suspended in 10 ml phosphate-buffered saline (PBS) and filtered using a 0.45-μm filter (Millex-AA, Merck Millipore, Darmstadt, Germany). The filtered viral particles were centrifuged (8,500×g ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Cell Biology 2021Quote: Human immortalised articular chondrocyte C28/I2 cells were purchased from Merck Millipore and cultured in DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: The human brain microvascular endothelial cell line hCMEC/D3 (Merck Millipore) was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... When stained for human nuclei (MAB1281, Merck Millipore, Fig. 4C-D), this was performed for 30 min at RT in the dark after EdU staining (Click-iT Assay ...
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bioRxiv - Microbiology 2020Quote: ... and affinity-purified rabbit polyclonal antibody against human CPSF6 (HPA039973; Merck) were used at a dilution of 1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: The HCYTOMAG-60K-15 Human Cytokine/Chemokine Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 IU mL1 human chorionic gonadotropin (hCG; Chorulon; Merck Animal Health) and 1.0 mg mL1 oestradiol ...
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Developmental Biology 2023Quote: ... Coating was performed with 20 µg/ml human fibronectin (Merck FC010) in PBS for at least 30’ ...
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bioRxiv - Cell Biology 2024Quote: ... recombinant full-length human active AAK (#14-511 Merck Millipore, Germany) were incubated either in the presence or the absence of 14nM of N-terminal GST-fused active HsMPS1/TTK (SignalChem ...
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bioRxiv - Biochemistry 2019Quote: Insulin and amylin concentration in plasma were determinate by ELISA (Insulin, Cat # EZRMI-13K and Amylin, Cat # EZHA-52K, Merck Millipore) following manufacturer manual ...
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bioRxiv - Molecular Biology 2022Quote: ... For application in ELISA elution fractions were pooled via Amicon Ultra-15 50k centrifugal filter units (Merck Millipore, Burlington, MA, USA). Elution buffer was chosen for the intended application (coating buffer for sandwich ELISA ...
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bioRxiv - Cell Biology 2023Quote: ... post-fixed in cooled ethanol:acetic acid (2:1) and stained using a TUNEL kit (ApopTag® Fluorescein In Situ Apoptosis Detection Kit, Merck Millipore, #S7110) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) (#D9542, Merck. Darmstadt. Germany).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... All routine cultures were performed in 6-well plates (Greiner, 657160, Merck, Darmstadt, Germany) at 37°C in 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... concentrated to 6 mg/ml by a 100-kDa cut-off Centricon (Merck Millipore), and flash frozen for further cryo-EM grid preparation.
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bioRxiv - Microbiology 2022Quote: ... The sample was loaded onto a Superose® 6 Increase 10/300 GL (Merck) column ...
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bioRxiv - Microbiology 2024Quote: ... and 300 nM 4′,6′-diamidino-2-phenylindole (DAPI) (Merck, Germany; #28718-90-3). Fluorescence signals were observed by an ECLIPSE TE2000-U fluorescence microscope (Nikon ...
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bioRxiv - Cancer Biology 2021Quote: ... Calibration of the cantilever was done on a surface of an empty petri dish filled with a Leibovitz’s L-15 medium without L-glutamine (Merck KGaA, Darmstadt, Germany) pre-warmed to 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... and xylazine/Rompun® (#770-081, Bayer, Leverkusen, Germany) and transcardially perfused with sterilized phosphate buffer saline (PBS, #D8537, Sigma, part of Merck, Darmstadt, Germany). The whole brain samples were removed and prepared into cell suspension using brain tumor dissociation kit (#130-095-942 ...
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bioRxiv - Molecular Biology 2021Quote: ... the reaction mixture was desalted three times and concentrated by ultrafiltration (Ultracel 100 kDa Ultrafiltration disc with 100 mM phosphate buffer, pH 6.9, Merck Millipore, Burlington, MA, USA) to a final volume of 12 ml.
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bioRxiv - Neuroscience 2023Quote: ... Cells were passaged twice a week using 2 mM ethylenediaminetetraacetic acid in phosphate buffer saline (EDTA/PBS; Thermo Fisher Scientific, Waltham, USA; Merck, Darmstadt, Germany) adding 10 µM Y27632 (Miltenyi Biotec ...
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bioRxiv - Biochemistry 2020Quote: ... 500 μM ATP and ~0.5 μg commercially available human CyclinB-Cdk1 (Merck). The reaction was carried out at 30 °C and was stopped using SDS-PAGE sample buffer after 5 minutes.
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bioRxiv - Cell Biology 2019Quote: ... Human pancreatic organoids were additionally immuno-stained against Sox9 (primary antibody: Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... a rabbit anti-human EGFR monoclonal antibody (EMD Millipore-MERCK, Temecula, USA) diluted 1:1000 or a rabbit anti-human VEGF-A polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... human tau constructs were expressed in the vector pNG2 (Merck-Novagen, Darmstadt) in E ...
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bioRxiv - Immunology 2021Quote: ... MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) were used ...