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Citations for Merck :
401 - 450 of 1157 citations for Chikungunya Virus E1 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The protein was concentrated using an Amicon Ultra 10 K device (Merck Millipore), loaded on a size exclusion column [ENrich SEC 650 10 x 300 Column (Bio-Rad)] ...
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bioRxiv - Microbiology 2021Quote: ... Purified protein was further concentrated using Amicon Ultra Centrifugal Filter Units (Merck Millipore).
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bioRxiv - Immunology 2021Quote: ... proteins were concentrated on a 0.5 mL 10 kDa filter (UFC501024, Merck-Millipore) by ultracentrifugation and purity was controlled by SDS-PAGE.
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bioRxiv - Plant Biology 2019Quote: ... All protein concentrations were determined using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... proteins were electro-transferred to a polyvinylidene difluoride membrane (Merck Millipore, Darmstadt, Germany), which was incubated in the blocking buffer (5% non-fat milk ...
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bioRxiv - Plant Biology 2019Quote: ... using protein at 18 mg/ml (measured by Direct Detect® spectrometer (Merck)) ...
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bioRxiv - Cell Biology 2020Quote: ... Immunoreactive proteins were visualized by Immobilion western chemiluminescence substrate (Merck-Millipore cat#WBKLS0500) [7].
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bioRxiv - Cancer Biology 2020Quote: ... The protein fractions were isolated and concentrated using 3kDa centrifugal filters (Merck Millipore). LC MS/MS was performed on the concentrated culture CMed and spectral analysis was performed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Total proteins were spotted (10 μg) onto nitrocellulose membranes (Merck Millipore, Darmstadt, Germany) and left to dry ...
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bioRxiv - Immunology 2021Quote: 5 µl of 10 µm bead slurry (PureProteom Protein A magnetic beads, Merck) were used per reaction ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were concentrated to > 150 μM using centrifugal filters (Amicon Ultra; Merck Millipore), aliquoted and snap-frozen and stored at −80 °C ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred to a PVDF membrane (Immobilon-FL; Merck Millipore, Billerica, MA). Immunoblotting was performed with primary antibodies against Camk2a phosphorylated at pT286 (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were incubated with Protein A Sepharose beads (Merck, GE17-5280-01) for 1 h at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... All protein concentrations were determined using a Direct Detect® Infrared Spectrometer (Merck).
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bioRxiv - Microbiology 2020Quote: ... Proteins were detected by Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, MA, USA) and a LAS-3000 mini chemiluminescence imaging system (Fujifilm ...
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bioRxiv - Plant Biology 2021Quote: ... the hybridization with proper antibodies by SNAP i.d 2.0 Protein Detection System (Merck) was performed ...
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bioRxiv - Neuroscience 2021Quote: ... proteins were transferred onto low fluorescent Immobilon-FL membrane (0.45μm por size, Merck) using the Biorad wet-blot system (1.15h ...
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bioRxiv - Microbiology 2021Quote: ... Eluted protein was desalted using PD-10 desalting columns (Sigma-Merck, U.S.A/Germany). Concentration of the desalted protein was estimated using Bradford’s method and the protein was stored at -80 °C until needed.
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bioRxiv - Microbiology 2021Quote: ... and the bacterial pellets were resuspended in BugBuster protein extraction reagent (Merck, Germany). The cell suspensions were incubated on a rotary shaker for 20 minutes ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... the protein was concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). A90C α-synuclein was labelled with 1.5-fold molar excess Alexa Fluor 488 C5 maleimide (Life Technologies ...
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bioRxiv - Molecular Biology 2022Quote: ... The resolved proteins were transferred onto a 0.45 m PVDF membrane (Immobilon, Merck) in ice-cold transfer buffer (25 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... The protein was concentrated using 10kDa Amicon filter (Merck Millipore, Burlington, MA, USA). The protein concentration in each fraction was estimated using the Bradford reagent (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The purified proteins were then concentrated using AmiconTM Ultra 15 concentrators (MERCK Millipore) and stored in -80°C.
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bioRxiv - Cell Biology 2022Quote: ... Protein transfer to PVDF membranes (Immobilon-P, 0.45 µm pore size, IPVH00010, Merck) was achieved using a wet transfer system (Bio-Rad) ...
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bioRxiv - Neuroscience 2021Quote: ... Protein concentration was estimated using the Direct Detect® Infrared Spectrophotometer (Merck-Millipore). 100 µg protein where loaded on a gel to detect endogenous MDGA proteins in Western blots ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... at 1:5,000 through SNAP i.d.® 2.0 Protein Detection System (C73105, Merck). This apparatus has a vacuum-driven technology and a built-in flow distributor that actively drives reagents through the membrane.
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bioRxiv - Cancer Biology 2020Quote: ... 60 μL of Magna ChIP™ Protein A+G Magnetic Beads (Merck Millipore) was used in accordance with the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... and tyrphostin A47 (a tyrosine protein kinase [TPK] specific blocker; Merck, Darmstadt, Germany). For each antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... denatured proteins were loaded onto 30 kDa amicon filter (Merck Millipore, Darmstadt, Germany). The buffer was exchanged with UA solution (8 M UREA in 0.1 M TEAB pH 8.5 ...
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bioRxiv - Biochemistry 2021Quote: ... imidazole was removed and the protein was concentrated using a Centricon (Merck Millipore) and preserved at −80 °C in small fractions at 2 mM concentration.
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bioRxiv - Neuroscience 2020Quote: ... Proteins separated in the gel were transferred to a PVDF membrane (Merck Millipore) using a submerged blotting apparatus and then visualized using Immobilon Western Chemiluminescent HRP Substrate (Millipore) ...
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bioRxiv - Immunology 2022Quote: ... The immunocomplex was captured by adding Protein G Agarose (Merck Millipore, Darmstadt, Germany). The protein binding to the beads was boiled in 2 x Laemmli sample buffer (Bio–Rad ...
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bioRxiv - Microbiology 2022Quote: ... Protein pellets were resuspended in 400 µl of 2.5% sodium deoxycholate (SDC; Merck) in 0.2 M EPPS-buffer (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein extracts were quantified using Direct Detect® Assay-free Cards (Merck; DDAC00010) or Pierce Bicinchoninic Bcid (BCA ...
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bioRxiv - Cell Biology 2019Quote: ... Eluted protein was concentrated using Amicon Ultra 15 centrifugal filters (Merck, Darmstadt, Germany) and separated on a Superose 6 Increase column (GE Healthcare Life Sciences ...
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bioRxiv - Biophysics 2019Quote: ... Protein containing fractions were concentrated over regenerated cellulose filters (Amicon, Merck, Darmstadt, Germany), exchanged into measurement buffer (TBS-Ca ...
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bioRxiv - Cancer Biology 2019Quote: ... proteins were transferred from gel to a PVDF membrane (Merck, cat. no. IPSN07852). Transfer was carried out for 45 minutes at 110V in transfer buffer (cold room) ...
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bioRxiv - Plant Biology 2020Quote: ... His-tagged proteins were washed and purified using the Amicon Pro System (Merck) according to manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: ... Protein bands were revealed by enhanced chemiluminescence reagents (ECL™ Prime, Merck Millipore) in a ChemiDoc XRS+ imaging system (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... and proteins were transferred onto polyvinylidene difluoride (PVDF) membranes (Merck Millipore, Chengdu, China) for 2 h at 150 mA in 10% transfer buffer [3% Tris–HCl (Aladdin Industrial Corporation ...
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bioRxiv - Immunology 2021Quote: ... Proteins were eluted from beads using 40 µg of 3x FLAG peptide (Merck) for 200µL of IP sample incubated on a rotator for 45 min at 4°C.
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bioRxiv - Immunology 2020Quote: ... Protein was transferred to PVDF membranes (Immobilon-P PVDF .45um, Merck Chemicals BV). Membranes were blocked in 5% fat-free milk (Elk ...
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bioRxiv - Molecular Biology 2019Quote: ... The proteins were concentrated using Amicon Ultracel-30 Centrifugal Filter Units (Merck Millipore) for wild-type RF2 and RF2-GGP and Ultracel-3 for ResQ62-His6 hereafter referred to as ResQ.
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bioRxiv - Biochemistry 2021Quote: ... The cleaved protein was loaded onto a 5 mL HisTrap FF column (Merck); the flow-through ...
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bioRxiv - Microbiology 2021Quote: ... before protein expression was induced O/N by adding 0.5 mM IPTG (Merck). The next day ...
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bioRxiv - Microbiology 2021Quote: ... before protein expression was induced O/N by adding 1 mM IPTG (Merck) and 4 ml of 36 % glucose (w/v) ...
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bioRxiv - Molecular Biology 2021Quote: ... Crude protein extract was incubated with Anti-FLAG® M2 Magnetic Beads (Merck) overnight ...
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bioRxiv - Immunology 2020Quote: ... followed by binding of protein A/G magnetic beads (Merck Millipore cat. LSKMAGAG02) for 4 h at 4℃ ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were then electrotransferred onto a polyvinylidene fluoride (PVDF) membrane (0.45 μm) (Merck by ice-cold liquid transfer for 2 h at 70 V ...