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Citations for Merck :
351 - 400 of 2578 citations for Recombinant Human Chemokine C X C Motif Ligand 10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... The mixture was then centrifuged at 15,000 g at 4 °C and the supernatant was filtered with a 0.22 μm filter (Merck Millipore). A Ni-NTA column was used to purify the recombinant GB1-VpmR-S1 on an AKTA system (GE) ...
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bioRxiv - Neuroscience 2022Quote: ... and plated in glass-coverslips (3000-4000 cells/well) pre-coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Cell Biology 2022Quote: ... and seeded in 48-well plates (3000-4000 cells/well) previously coated with 0.1mg/ml poly-D-lysine (2h, 37°C; Merck) and 2μg/ml laminin (over-night (O/N) ...
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bioRxiv - Genetics 2022Quote: ... The tissue sections were then dried for 5 min at 37°C and mounted with 85% glycerol (Merck, 104094) and a coverslip ...
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bioRxiv - Physiology 2022Quote: ... containing 150 μL 4°C DMEM (Dulbecco’s modified Eagle’s medium with high glucose and 25mM HEPES from Sigma Aldrich (Merck), UK ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were centrifuged (16000 g, 20 min, 4°C) and filtered using Ultrafree Centrifugal Filter Units (Merck Millipore, USA).
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bioRxiv - Physiology 2020Quote: ... Membranes were probed overnight at 4°C with anti-phosphotyrosine antibody (1/1000, 05-321, clone 4G10, Merck Millipore) in blocking solution ...
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bioRxiv - Plant Biology 2019Quote: ... and cell debris were removed by centrifugation (2x 30 min, 20000 g, 4 °C) and filtering through 0.45 µm syringe filters (Merck).
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bioRxiv - Microbiology 2021Quote: ... The diced tissue was then digested for 60 minutes at 37°C in 12ml of a pre-heated suspension of collagenase I (2.4mg/ml, Merck) and DNase I (1mg/ml ...
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bioRxiv - Genomics 2022Quote: ... Total chromatin was pre-cleared for 1h at 4°C with rotation using protein A sepharose beads (P9424, Merck). 3 µg of anti-histone H3 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... with 1μg/mL anti-CD3 and 1μg/mL anti-CD28 at 4°C at a density of 105 cells/m in starvation media (RPMI (Merck), containing 0.5% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... The virus-containing lysate was purified via iodixanol density-gradient ultracentrifugation (290,000 × g, 120 min, at 4°C) and rebuffered in PBS using amicon spin columns (Merck). Particles in PBS were stored in aliquots at −80°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Lysates were collected at 20,000 x g for 40 min at 4°C and soluble proteins in the supernatant were sterile filtered 5µm PVDF Membrane (Merck).
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bioRxiv - Immunology 2022Quote: ... The sections were incubated overnight at 4°C with the following primary antibodies: KRT8 (Merck Millipore, MABT329, 1:100), p-ERK (Cell Signaling Technology ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted 1:400 v/v in 0.5% low gelling temperature agarose in Ames’ medium at 37°C (A1420; Merck). A thin film was polymerized over a pure agarose meniscus in a Petri dish and covered with Ames’ medium ...
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bioRxiv - Microbiology 2024Quote: ... samples maintained at 4°C were eluted through a ZIC-pHILIC column (5 μm, polymeric, 150 by 4.6 mm; SeQuant, Merck) by mobile phase A (20 mM ammonium carbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... The lysate was then spun at 20,000 g for 20 min at 4°C and loaded to a HisTrap HP column (Merck). The protein was eluted with the buffer with 300 mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The reactions were incubated at 37 °C for 1 h and subsequently stopped by addition of 1x PhosStop (Merck). In vitro phosphorylation of dephosphorylated recombinant SYNJ2BP (0.5 µg in 10 µl ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were stored for 24 h at 4°C in 4% PFA and then washed and stored in PBS containing 0.05% sodium-azide (Merck). Within a week of perfusion ...
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bioRxiv - Molecular Biology 2023Quote: ... Chromatin was enzymatically sheared for 5 min at 37°C with 0.2 mU/μL Micrococcal nuclease (MNase) (#N3755, Merck), and stopped with MNase stop buffer (110 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... 4561086) and transferred at 4°C to either Immobilon-FL or Immunoblon-P (IPVH00010) polyvinylidene fluoride membranes (Merck, IPFL00010) (1.5 hours ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated at 37°C for 1 hour with 10U/ml DNase (Benzonase, Merck-Millipore via Sigma-Aldrich) followed by centrifugation ...
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bioRxiv - Cancer Biology 2023Quote: ... was added for 16-18 hr at 4°C before washes and the addition of anti-phospho H2A.X mAb (Merck, Kenilworth ...
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bioRxiv - Biophysics 2023Quote: ... Poly-D-lysine coating was done by incubating the wells for 20 minutes at 37°C in a 0.4 mg/ml solution (4X) of poly-D-lysine (Merck, P7280-5MG ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 8.0) and then transferred to 1.5 mL tubes prewarmed at 65°C acid-washed glass beads (G8772-100 Merck) suspended in 25 µL of 10% filtered SDS and 300 µL of acid phenol chloroform pH 4.5 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Microbiology 2024Quote: ... −80 °C) onto a solid medium (supplemented with 1.5 % agar) and growing for four days in anaerobic jars (Anaerocult, Merck). Liquid cultures were obtained by inoculation of three separated colonies into 10 mL of liquid medium in 15 ml Greiner tubes and propagation for three days at 30 °C without shaking ...
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bioRxiv - Immunology 2024Quote: B cell lines used as target cell lines (i.e. Raji and MEC1) were grown at 37°C and 5% CO2 in RMPI-1640 medium (Merck) supplemented with 7.5% iron-enriched BCS (GE Healthcare HyClone).
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bioRxiv - Microbiology 2024Quote: ... Supernatant aliquots were kept at -18°C before being diluted with 2.5 mM sulfuric acid (H2SO4, Merck, Darmstadt, Germany) at 1:6 ...
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bioRxiv - Physiology 2024Quote: ... Membrane was incubated overnight at 4°C with specific mouse monoclonal primary antibody anti-puromycin (Merck, MABE343, 1:1000) and after wash ...
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bioRxiv - Genetics 2024Quote: ... The harvested medium was then centrifuged at 1,300 rpm at 4 °C for 5 min to remove cells and then filtered by 0.45 μm filter (Merck) and the virus was collected and stored at -80 °C.
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bioRxiv - Genomics 2022Quote: ... females and males were primed with 20 U and 10 U human chorionic gonadotropin (hCG, Pregnyl, Merck), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Serum cytokine profiling targeting 22 cytokines/chemokines was performed by Public Health England using a 22 -plex customized Luminex kit (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Neuroscience 2021Quote: The concentration of 9 cytokines and chemokines with well-characterised roles in innate immune responses were measured in maternal plasma by a Multiplex/Luminex (Merck, MCYTMAG-70K-PX32) according to the manufacturer’s instruction ...
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bioRxiv - Physiology 2022Quote: ... the mixture was centrifuged for 10 min at 17,000 x g before transferring the mixture to an NMR tube (Merck) for subsequent NMR analysis.
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bioRxiv - Plant Biology 2021Quote: ... NaOCl and 0.02% (v/v) Triton X-100 for 10 min and washed five times with ultrapure water (Milli-Q; Merck). Following seed stratification at 4°C for 2 d ...
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bioRxiv - Immunology 2020Quote: ... β2m and other contaminants using a C18 reversed-phase HPLC column (4.6 mm internal diameter x 10 cm, Chromolith Speed Rod, Merck) running on a mobile phase buffer of buffer A (0.1% trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2022Quote: ... cells were permeabilized with 0.1% Triton X-100 for 3 minutes and then blocked with 5 or 10% bovine serum albumin (BSA, Merck) in PBS for 20 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... The chromatographic separation of anionic phospholipids was performed on a reverse phase C18 column (SUPELCOSIL™ ABZ PLUS; 10 cm x 2.1 mm, 3µm, Merck) using methanol:water (3:2 ...
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bioRxiv - Plant Biology 2024Quote: ... each) onto a silica gel TLC plate (60 F254, 20 x 10 cm, 0.2 mm thickness; E. Merck, Germany) with the Linomat V sample applicator ...
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bioRxiv - Evolutionary Biology 2020Quote: ... at 4°C in dialysis buffer (100 mM NaCl, 20mM Tris pH 7.4, containing 15 μl bovine thrombin (605157, Merck Millipore) to cleave the His-tags) ...
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bioRxiv - Microbiology 2020Quote: ... After 2 hours at 30°C with shaking 1 ml of cells was pelleted and soluble proteins were extracted using BugBuster (Merck). For internalisation experiments involving plasmid-complemented deletion strains ...
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Molecular Biology 2021Quote: Heat-mediated antigen retrieval was performed for 3 min at 99°C in a 40mM trisodium citrate (Merck, Darmstadt, Germany) solution ...
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bioRxiv - Cell Biology 2022Quote: ... Lysate was spun at 19650 x g for 20 min at 4 °C and the cleared supernatant was filtered in a 0.45 μm filter (Merck Millipore). M2 magnetic FLAG beads (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2019Quote: ... Membranes were blocked in 5% milk in TBS-T and incubated overnight at 4°C with the primary antibodies anti-tyrosine hydroxylase (1:1,000; AB152; Merck Millipore) or anti-dopa decarboxylase (1:500 ...
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bioRxiv - Molecular Biology 2020Quote: ... Three individual clones were picked and amplified in LB broth including antibiotics for 12 h at 37°C and isolated using GenElute– HP Plasmid MaxiPrep Kit (Merck). Integrity of purified expression constructs was validated by gel electrophoresis and the concentration was determined by absorbance measurement ...
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bioRxiv - Microbiology 2019Quote: ... coli strain strains were grown at 37°C in Lysogeny broth (LB) with shaking at 200 rpm or on agar (Merck). All meningococcal strains were grown on GC agar plates or GC broth (Difco ...
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bioRxiv - Cancer Biology 2020Quote: ... 4°C) and the EV-depleted supernatant was filtered through a 0.22 μm pore PVDF Millex-HV filter (Merck Millipore) and analysed by NTA ...
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bioRxiv - Neuroscience 2021Quote: ... After washing the sections were incubated overnight at 4°C with anti-NeuN antibody (1:1000, Merck Millipore, Cat. # ABN90P) in 1% normal goat serum and 0.1% Triton-X-100 in TBS ...