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Citations for Merck :
351 - 400 of 1371 citations for Ectonucleotide pyrophosphatase phosphodiesterase family member 7 ENPP7 Antibody HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Protein signals on the membranes were detected with Immobilon Forte Western HRP substrate (Merck, Darmstadt, Germany) and the ChemiImager Ready (Alpha Innotec,San Leandro USA).
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bioRxiv - Molecular Biology 2021Quote: ... before being incubated for 5 secondes in Luminata Classico Western HRP substrate (Merck Millipore, Molsheim, France) and then imaged for chemiluminescence using the ChemiDoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Plant Biology 2022Quote: ... The water was then replaced with a solution containing 10 μg/ml horseradish peroxidase (HRP) (Merck), 100 μM of the luminol derivate L-012 (Wako Chemicals ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-mediated substrate development was performed using Amersham detection system (ECL Western Blot detection kit, Merck). Blots were imaged using ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed with TBS-T and developed using Luminata Forte Western HRP substrate (Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase activity was detected with enhanced chemiluminescence (Luminata Crescendo Western HRP, Cat. No. WBLUR0500, Merck, Germany). Membranes were stripped using Restore Western Blot Stripping Buffer (Cat ...
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bioRxiv - Neuroscience 2024Quote: ... membranes were developed using Fusion Fx (Vilber Lourmat) with Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore).
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies: anti-LC3 antibody Atg8 (Merck Millipore #ABC975) (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... was transfected into COS-7 cells cultured in a well of 8 well chamber slide glass using GeneJuice Transfection Reagent (Merck Millipore) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... samples were resuspended in Urea 7 M and Thiourea 2 M buffer and desalted on Amicon Ultra-0.5 3 kDa centrifugal filters (Merck Millipore, Germany). Filters were filled to maximum capacity with buffers and centrifuged at 15,000 g for 10 min at 20°C ...
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bioRxiv - Microbiology 2023Quote: ... The flow-through was sterile filtered and concentrated to a final volume of 7 ml using Amicon Ultra-15 Centrifugal filter concentrator (Merck Millipore).
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bioRxiv - Immunology 2023Quote: ... The digested suspension was pelleted and the pepM extract buffer exchanged to PBS pH 7 using 10 kDa Amicon centrifugal filter unit (Merck, Ireland). PepM extracts were confirmed with SDS-PAGE and stored in solution at − 20°C.
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2022Quote: ... and additional wells with 20% biotinylated PLL-g-PEG and FNIII(7-10) were supplemented with 1 µM staurosporine (Merck Millipore) to serve as a positive control for apoptosis ...
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bioRxiv - Cancer Biology 2022Quote: ... The purified protein was dialyzed overnight in 25 mM Tris-Cl pH 7.5 using SnakeSkin®ialysis tubing (7 kDa molecular weight cutoff) and concentrated using Amicon™ Ultra-15 Centrifugal Filter Units (30 kDa molecular weight cutoff) (Merck) according to the requirements of the experiments.
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bioRxiv - Microbiology 2022Quote: A CRISPR array containing 6 identical spacers targeting the tetR gene flanked by 7 repeats was cloned into pCDF-Duet (Novagen, Merck Millipore) by ligation after NcoI and SalI digestion ...
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bioRxiv - Bioengineering 2024Quote: ... Samples used for microscopy observations in Figure 7 were purified using tangential ultrafiltration with Amicon® 100kDa 0.5ml (Merck Millipore®) in order to avoid the dilution resulting from SEC ...
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bioRxiv - Microbiology 2024Quote: Sucrose gradient purified fractions of bioreactor-derived PV-2 SC6b and PV-2 SC5a were pooled and concentrated with buffer exchange into PBS + 20mM EDTA (pH 7) using Amicon Ultra centrifugal filter devices (100 kDa MWCO, Merck Millipore) to a final concentration of 0.1 mg/ml (PV-2 SC6b ...
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bioRxiv - Immunology 2021Quote: ... Target protein expression was detected using Immobilon™ Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... Immuno-reactive proteins were visualized using the Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Burlington, MA, USA). The detected bands on the scanned blots were quantitated with ImageJ freeware.
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bioRxiv - Microbiology 2023Quote: ... Goat Anti-Rabbit IgG HRP conjugate (632131/Merck/Massachusetts, USA, 1:10,000 were procured from Merck (USA). Mouse ß-Actin (3700S/ 1:1000/Cell Signalling technology/Massachusetts ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibody (Merck guinea pig anti-NeuN 1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... Proteins in the serum were removed by passing it through a pre-rinsed (7 times washed) Amicon Ultra-2ml 3000 MWCO (Merck Millipore, USA) column ...
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bioRxiv - Neuroscience 2020Quote: ... The cortices were homogenized mechanically in PBS by pipetting up and down with a 5mL plastic pipette, centrifuged (500 x g, 7 min, 4 °C) and resuspended in DMEM (Sigma-Aldrich, Merck, Switzerland) containing 5% FCS (Biochrom ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... 50 µL benzaldehyde (BA) and 250 µL 3-octanol (OCT) (CAS 100-52-7, and 589-98-0, respectively; Merck, Darmstadt, Germany) were applied undiluted to 1-cm-deep Teflon containers of 5 and 14 mm diameter ...
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bioRxiv - Molecular Biology 2021Quote: ... the protocol described by Choi et al.65 was applied using home-made wash buffer (50% formamide (Merck Millipore, 75-12-7), 5XSSC (Molecular Biology-P ...
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bioRxiv - Genomics 2023Quote: ... These assessments were conducted on 7-day old Foc strains cultured on petri dishes with potato dextrose agar (PDA; Merck, Darmstadt, Germany). An approximately 5 mm piece of the actively growing colony from each isolate was placed on PDA plates and incubated at 27°C ...
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bioRxiv - Microbiology 2022Quote: ... Five duplicate plates were spread individually by six gradient diluted soil suspensions from 10−2 to 10−7 dilution on 1/10 TSA medium (Tryptic Soy Agar, Merck, Darmstadt, Germany) and incubated under both aerobic and anaerobic conditions at 28 °C for two weeks ...
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bioRxiv - Neuroscience 2023Quote: ... after testing multiple antibodies (anti-NeuN rabbit Antibody, ABN78 & ABN78C3, Merck; anti-NeuN rabbit Antibody ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies used were mouse anti-EGFR antibody (#GR01; Merck Millipore), goat anti-EEA1 (#sc-6414 ...
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibody RNA-DNA hybrid (s9.6) antibody (Merck Millipore, #MABE1095, 3ul) or GFP (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore).
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Bioengineering 2021Quote: ... Binding of biotinylated S1-RBD to coated VNAR-hFc was detected by streptavidin-HRP (Merck Millipore, 18-152). The signal intensities were collected for each primary VNAR-hFc antibody without competitor and compared to the signal when a competitor antibody was present ...
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bioRxiv - Molecular Biology 2022Quote: ... Immunodetection was performed using a Luminata Forte Western HRP Substrate system (Merck Millipore, Burlington, USA, 61-0206-81) or a Chemi-Lumi One L system (Nacalai Tesque ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed 3 times with PBS-T for 5 min and incubated with mouse-HRP (A4416; Merck) and rabbit-HRP (GENA9640V ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP (AP182P, Merck Millipore) or donkey anti-mouse IgG HRP (AP192P ...
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bioRxiv - Immunology 2023Quote: Immunoblotting was performed as described above and MIF-6xHis-biotin revealed using streptavidin-HRP-conjugate (Merck, Darmstadt, Germany), while MDL1-6xHis was revealed using a custom-made monoclonal primary antibody (1:10 dilution) ...
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bioRxiv - Microbiology 2023Quote: ... were used at a 1/10000 dilution before reaction with Immobilon Forte Western HRP substrate (WBLUF0100, Merck Millipore). Signals were acquired with Fusion FX (Vilber).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Immunology 2022Quote: ... the cells were transferred to a single well containing a monolayer of mitomycin C (Cat no. 50-07-7, Millipore Sigma, Merck, Darmstadt, Germany)-treated OP9/ OP9-DL1 cells or were kept unaltered ...
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bioRxiv - Biochemistry 2021Quote: ... a biotinylated DNA oligonucleotide complementary to the target tRNA (Supplementary Table 7) was immobilized on Novagen Streptavidin Agarose resin (Merck-Millipore, Burlington, MA) in a 0.22 μm Ultrafree-MC filter cup (Merck-Millipore ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 mM EDTA) with 1 µl lyticase (17 U/ µl in 10 mM KPO4 pH 7, 50% glycerol, Merck >2000 U/mg L2524), heated to 50°C for 2 min before addition of 40 µl molten CleanCut agarose (Bio-Rad 1703594) ...
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bioRxiv - Biochemistry 2024Quote: Mixing of lysozyme crystals (7×2 mm; grown by batch crystallisation) with 25 mM sulfanilic acid azochromotrop (SAA, Merck, lmax 505–510 nm), a highly absorbing red dye ...
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bioRxiv - Molecular Biology 2022Quote: ... was placed on the top of a water-cooled glass column (33 × 2.5 cm) filled with a slurry of silica gel 60 (with the addition of 7 % water, 40 – 63 μm, Merck, Darmstadt, Germany, # 1.09385.2500) and n-pentane ...
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bioRxiv - Bioengineering 2024Quote: ... was assessed every day for 7 to 10 days using a Millicell ERS-2 voltammeter EVOM2 and an STX02 chopstick electrode (Merck Millipore, Bayswater Australia). The TEER value was calculated by subtracting the resistance of a blank insert from an insert containing a monolayer and then multiplying it by the surface area of the insert.
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bioRxiv - Molecular Biology 2022Quote: ... incubated with the primary antibodies (see the list of antibodies in Table S2) and with an anti-rabbit secondary antibody (Merck), and visualized by chemiluminescence substrate (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... antibodies (α-GluA2, Merck clone 6C4 ...
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bioRxiv - Immunology 2020Quote: ... antibodies in RPMI (Merck), containing 10% Heat Inactivated FCS (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies (NaV1.1 Merck, AB5204 ...