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Citations for Merck :
351 - 400 of 439 citations for 6 amino 7H benzo e perimidin 7 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 15 µg of total protein in a volume of 47 µl 1x sample buffer was treated with 7 U of benzonase (#70746; Merck Millipore, Burlington, MA, USA) in dilution buffer (20 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... [2H7]22R-Hydroxycholest-4-en-3-one ([2H7]22R- HCO) was prepared from [2H7]22R-HC by treatment with cholesterol oxidase enzyme (Streptomyces sp., Merck, Dorset, UK) (11).
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bioRxiv - Neuroscience 2020Quote: ... Blots were washed and probed with secondary antibody for one hour at RT (anti-mouse IgG, horseradish peroxidase linked; 1:5,000; Merck, Burlington, MA, USA). Bands were visualized using high sensitivity Pierce ECL Plus (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... The residue was then resuspended in 100 µl methanol and one µl of each sample analysed by thin layer chromatography (TLC) using silica gel 60 F254 (Merck Millipore, Germany) with chloroform-acetic acid-ethanol (90:5:10 [vol/vol] ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
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bioRxiv - Microbiology 2019Quote: ... 6 μl of the reaction was spotted on foil backed silica plate (Silicagel 60, 20 x 20, Merck) and develop in butanol:acetic acid:water 2:1:1 (mobile phase) ...
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bioRxiv - Systems Biology 2020Quote: ... 400 µl were either filled into three wells of a 6-well TPP® tissue culture plate (Merck) supplemented with 0.5 % glucose and incubated under static conditions or used for inoculation of 3 × 10 mL TSB ...
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bioRxiv - Biochemistry 2020Quote: ... Human Rab8a 6-176aa (referred to as Rab8a)-encoding DNA was cloned into a pet51b(+) vector (Merck Millipore), resulting in a construct with a N-terminal Strep® II tag and enterokinase cleavage site and a C-terminal 10xHis tag ...
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bioRxiv - Cell Biology 2022Quote: ... then brought to 10 ml with ddH2O) and ECL2 solution (6 µl 30% (v/v) H2O2 (Merck, #H1009) in 10 ml with ddH2O ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were harvested 6 hours post transfection through the addition of 1 mL TRI reagent (Sigma-Aldrich, Merck) directly to the monolayer ...
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bioRxiv - Cell Biology 2023Quote: ... beta-galactosidase solution was prepared containing 20 mM citric acid (pH=6) (ref 1.00244.0500, Merck Millipore, Darmstadt, Germany), 5 mM of potassium hexacyano-ferrate (II ...
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bioRxiv - Microbiology 2020Quote: ... One hundred mL of sewage samples were filtered with a 0.22-μm-pore-size mixed cellulose ester membrane (Merck Millipore, Billerica, MA, USA). DNA was extracted from the filtered membranes and from 150–200mg of the nonhuman fecal samples using the ZR Fecal DNA MiniPrep™ Kit (Zymo Research ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
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bioRxiv - Microbiology 2024Quote: ... and the chloroform phase was separated by one-dimensional TLC on high performance TLC aluminum sheets (silica gel 60; Merck Poole, United Kingdom) and developed with chloroform/methanol/ammonium hydroxide (40:10:1 ...
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bioRxiv - Plant Biology 2020Quote: ... and the buffer changed to 6 M urea using Amicon ultra-15 centrifugal filter units with 10,000 MWCO (Merck). The protein concentration was determined spectroscopically at 280 nm on a NanoDrop™ spectrophotometer based on the Lambert-Beer’s law assuming a molecular weight of the CBC-Qprot of 47921 Da and an extinction coefficient of 37,400 M−1 cm−1 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with the nucleus DNA stain 4′,6-diamidino-2-phenylindole (DAPI) (1μg/ml D9542-10MG, Merck Life Science UK Ltd ...
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bioRxiv - Molecular Biology 2019Quote: ... 5 and 6 were pooled and concentrated to 1 ml using 10 kDa centrifugal filter (Amicon Ultra-2ml, Merck Millipore ...
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bioRxiv - Molecular Biology 2019Quote: ... from an 18500g centrifugation (6 min at +4°C) were run through 3-kDa cut-off centrifugal filters (Amicon Ultra-0.5 ml, Merck) into pre-weighted collection tubes to remove remaining macromolecules ...
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bioRxiv - Cell Biology 2021Quote: ... and/or with plasmid DNA (e.g. 1µg/well for 6-well plates) for 24 hrs using GeneJuice (Merck Millipore). siRNAs targeting USP7 ...
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bioRxiv - Cell Biology 2022Quote: ... and were stained on day 6 after the last 1-hour stimulus with dimethyl-methylene blue (DMMB; pH 1.8; Merck) metachromatic dyes as previously described 15 ...
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bioRxiv - Cell Biology 2019Quote: ... acetylated α-tubulin (Clone 6-11B-1, Cell signalling Technology) or tyrosinated α-tubulin (Clone YL1/2, Merck Millipore) in blocking solution at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... approximately 5×105 Calu-3 cells were pre-treated with kp7-6 (100 ug/mL, CD95/CD95L antagonist, Merck) for 2 hour and then infected with SARS-CoV-2 at an MOI of 0.2 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Neuroscience 2023Quote: ... mice were fasted for 6 hours prior to being given an IP bolus of 0.75 units/kg insulin (I9278, Merck). Blood was collected at 0 ...
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bioRxiv - Biochemistry 2023Quote: ... 3 x 105 cells per well were seeded into a 6-well plate in high-glucose Dulbecco’s modified Eagle’s (DMEM) medium (Merck, UK) enriched with 10% FBS (Sigma- Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were washed (6 x 300 µL PBST) and 100 µL of pre-warmed 3,3’,5,5’ tetramethylbenzidine (TMB) solution (Merck-Sigma) was added to each well ...
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bioRxiv - Biochemistry 2024Quote: ... Dissolved IntC-UbcH5c-CAET-Ub was transferred to a dialyzer (D-Tube Dialyzer Maxi, MWCO 6– 8 kDa, Merck) in 300 mL of unfolding buffer ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transfected with a total of 1000 μg to 1500 μg DNA in 35 mm fluorodishes or 6 well plates using Genejuice as per the manufacturer’s instructions (Merck Millipore). Cells were typically imaged 48 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: RNA for each biological repeat was extracted from 110 mg of rosette leaves number 5-6 (from at least six plants) with TRI Reagent (Merck) and rounds of phenol-chloroform and chloroform extractions followed by isopropanol precipitation ...
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bioRxiv - Developmental Biology 2019Quote: The multipotent mouse mesenchymal stem cells, C3H10T1/2 cells (Reznikoff et al., 1973) were cultured on 6-wells TPP plastic culture plates (Merck) or 6-wells Uniflex Flexcell plates (FlexCell Int ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 million cells were plated per well in a 6-well plate and cultured for 48h in the presence or absence of 200 nM smoothened agonist (Merck). RNA was extracted and cDNA was generated with Superscript II kit (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... were assessed by analytical gel filtration using an Akta FPLC UPC-900 equipped with a Superose 6 Increase 10/300 GL column (Merck). Typically ...
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... cells in E8 medium were seeded into a V-shaped 96-well plate (6 000 cells/well) coated with Polyhema (Merck). After 48 hours (Day 0 of the differentiation process ...
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bioRxiv - Microbiology 2021Quote: ... NHS-ester staining: the gel was placed in a 6 well plate and incubated in 594 NHS-ester (Merck, 08741) diluted at 10 μg/mL in 3 ml PBS for 1 hour and 30 minutes at room temperature on a rocking platform ...
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bioRxiv - Immunology 2020Quote: ... The thorax and the skin incision were closed with 6/0 silk sutures (Lorca Marín) and buprenorphine (0.01 mg/kg, Buprex, Merck & Co. Inc) was given for pain relief ...
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bioRxiv - Neuroscience 2022Quote: ... Following counterstaining with 4’,6-diamidino-2-phenylindole (DAPI; Vectashield/Biozol) slices were mounted in Mowiol 4-88 (Merck Chemicals).
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bioRxiv - Microbiology 2023Quote: R47 and R32 wild type strains were inoculated and grown in 6-well plates as described in the RNA extraction section except that 1 ml 0.38 M NaOH (Merck, Germany) was added to the middle two wells to capture HCN in the surrounding ...
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bioRxiv - Microbiology 2023Quote: ... the pH of the TSB was adjusted with hydrochloric acid solution (1M and 6 M HCL; Merck KGaA, Darmstadt, Germany) immediately prior to the experiment ...
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bioRxiv - Cell Biology 2023Quote: ... we seeded cells at 2 * 106 cells/ mL in S2 media in 2 mL media/ well in a 6-well plate with either DMSO (D2650, Merck), 2.5 μM CytD (C2618 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants (35 mL) were removed at day 6 and day 10 and filtered through a Miracloth (Merck Milipore, Carrigtwohill, Ireland) into a clean tube ...
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bioRxiv - Microbiology 2023Quote: ... LB plates were seeded with the different cultures and Whatman n° 1 filter discs (6 mm) were impregnated with 5 μl of 30% (v/v) H2O2 (Merck) as previously described [31] ...
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bioRxiv - Neuroscience 2024Quote: ... the myelinated slices were incubated 16 hours at 6 DIV in 0.5 mg/ml L-α-Lysophosphatidylcholine (LPC, 830071P, Avanti, Merck) added to fresh culture medium.
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bioRxiv - Molecular Biology 2023Quote: ... then the mixture was dialyzed against histone refolding buffer at 4 □ using a dialysis tube (D-TubeTM Dialyzer Medi or Maxi, MWCO 6-8 kDa, Merck). HE buffer (10 mM HEPES ...
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bioRxiv - Cancer Biology 2024Quote: ... Only treatment naive patients that underwent anti-PD-1 monotherapy with either pembrolizumab (200 mg IV every 3 weeks or 400 mg IV every 6 weeks, Merck) or nivolumab (240 mg IV every 2 weeks or 480 mg IV every 4 weeks ...
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bioRxiv - Genomics 2021Quote: ... Birds sacrificed on one DPI (I1, C1) were euthanized via lethal injection with pentobarbital sodium and phenytoin sodium solution (Beuthanasia-D Special, Merck Animal Health, Madison, NJ, USA), but due to complications with intravenous injections ...
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bioRxiv - Neuroscience 2020Quote: Samples boiled in 6× Laemmli buffer were subjected to standard SDS-PAGE (10%) and electrophoretically blotted onto Immobilon-P polyvinylidene difluoride membranes (Merck, UK). Total protein was quantified using Ponceau S staining (Merck ...
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bioRxiv - Plant Biology 2021Quote: ... Each exudate sample was purified using solid phase extraction (SPE) with C18 Discovery® cartridges (bed wt. 500 mg, volume 6 mL, Merck). Cartridges were activated using 5 mL acetone and washed with 5 mL distilled water ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and host plants was assessed by extracting the compounds following [6] by immersing the samples for 5 min in 5 ml of hexane (99%, SupraSolv, Merck, Germany), followed by removal from hexane with entomological tweezers that were previously cleaned with hexane ...
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bioRxiv - Biochemistry 2022Quote: ... were separated on 0.1% SDS polyacrylamide gels (6–12% w/v depending on the molecular mass of protein) and electroblotted onto Immobilon-PVDF Transfer Membrane (Merck Millipore). Blots were blocked in 5% w/v non-fat milk or 5% BSA (Albumin ...