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Citations for Merck :
3901 - 3950 of 4003 citations for Propane 1 2 Diyl Diacetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... HeLa S3 Flp-In host cells were co-transfected with pEF5/FRT/CTLA4-HA and pOG44 at a ratio of 1:9 using GeneJuice (Merck Millipore, 70967) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The combined aqueous phase gave a total volume of 450 mL of which 5 uL were measured by Method 1 and compared against freshly prepared authentic standards (Merck, Darmstadt, Germany). We wish to note that our acid quantification generated much higher absolute values than an earlier study (Szecowka et al ...
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bioRxiv - Microbiology 2023Quote: ... 50 µl of each dilution were mixed with 10 µl of 0.4 mg ml-1 4-methyl-umbelliferyl-β-D- galactopyranoside (MUG) substrate (Merck, Darmstadt, Germany) that was prepared in dimethyl sulfoxide (DMSO ...
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bioRxiv - Developmental Biology 2023Quote: ... the slides were rinsed three times with PBS and blocked for 1 hour at RT in blocking buffer made with 3% BSA (Merck Millipore, 0218072801) and 0.02% Triton X-100 (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/L, VWR; di-ammonium citrate 0.7 g/L, Sigma-Aldrich; sodium acetate 0.26 g/L, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Genomics 2023Quote: ... a ∼20%-confluent wild-type iPSC culture in a 12-well plate containing 1 ml/well of Essential 8 supplemented with 10 µM ROCK inhibitor (Merck, cat# Y0503) was co-transfected with the pZT-C13-L1 and pZT-C13-R1 plasmids (Cerbini et al ...
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bioRxiv - Neuroscience 2023Quote: ... was dissolved in vehicle (Ringer solution; NaCl 140 mM, CaCl21.2 mM, KCl 3 mM and MgCl2 1 mM (Merck KGaA Darmstadt, Germany)) to a dilution of 5.5μg/μl ...
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bioRxiv - Microbiology 2023Quote: ... K2HPO4 2.9 g/l, VWR; di-ammonium citrate 0.7 g/l, Sigma-Aldrich; sodium acetate 0.26 g/l, Merck; glucose 1% (w/v), Merck ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Cell Biology 2022Quote: ... GFP signals were amplified by immunostaining with polyclonal rabbit anti-GFP (used at 1:500, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany, SAB4301138). 40-1a (used at 1:50 ...
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bioRxiv - Biophysics 2023Quote: ... Amphipol-reconstituted P2X4 was pooled and concentrated to approximately 1 mg/mL using an Amicon Ultra 50 kDa cutoff (Merck Millipore, USA). All steps were performed at 4 °C.
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bioRxiv - Plant Biology 2023Quote: ... Sections were rinsed 6 times in MTSB and incubated with 1 µg/mL Calcofluor white (Fluorescent Brightener 28 disodium salt solution, Merck, in MTSB). After rinsing ...
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bioRxiv - Biochemistry 2023Quote: ... The 100-fold dilution of culture was made up to 10-4 dilutions and spotted on TYE plates (Hi-Media Laboratories Ltd., India) having 1% glucose (Merck & Co., USA) and ampicillin (Gold Biotechnology ...
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bioRxiv - Molecular Biology 2023Quote: ... 10% glycerol) supplemented with 1 mg/ml lysozyme and one tablet of SIGMAFAST Protease Inhibitor Cocktail Tablets EDTA-Free (Merck KGaA, Germany), and disrupted by sonication (10 cycles of 30 sec at 40 % intensity in ice ...
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bioRxiv - Cell Biology 2024Quote: ... Base elastomer was thoroughly mixed with curing agent at a ratio of a ratio of 10:1, before degassing in a vacuum desiccator (Scienceware, 999320237) with a KNF Laboport N96 diaphragm vacuum pump (Merck, Z675091-1EA). A Si wafer with positive features was placed in a disposable aluminium dish (VWR ...
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bioRxiv - Bioengineering 2024Quote: ... The scaffold was then rinsed three times with PBS and incubated with Alexa Fluor™ 555 conjugated secondary goat anti-mouse IgG antibody (1:100; Merck, Germany) and CF™ 633 conjugated secondary donkey anti-rat IgG antibody (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: A FLAG-tagged or HA-tagged cDNA fragment of DDX43 was amplified by RT-PCR from total RNA extracted from silkworm ovaries and cloned into the pIEx-1 vector (Merck Millipore/Novagen) or the pIExZ vector using an In-Fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2024Quote: ... The chlorine concentrate solution was prepared by addition of a NaOCl tablet (B-Care Chemicals, Belgium) to 1 L ultrapure water (Milli-Q, Merck Millipore, Germany). The amount of free chlorine was quantified with the Pocket Colorimeter II (Hach,Belgium).
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bioRxiv - Cell Biology 2024Quote: ... hTERT-RPE1-Flp-In-Parental cells were co-transfected with a 10:1 ratio of pOG44 and either pCDNA5 FRT/TO-NeoR-HA-VPS35-WT or -[D620N] using Genejuice (Merck Millipore; 70967), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Infection foci were visualised using the appropriate secondary antibody coupled with horseradish peroxidase (HRP; 1:5000, Sheep Anti-Mouse IgG A5906, Sigma-Aldrich, Merck, Germany) and TrueBlueTM chemical (SeraCare ...
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bioRxiv - Microbiology 2024Quote: ... Actin was detected with monoclonal mouse antibody coupled with horseradish peroxidase (HRP; 1:75,000; A3854, Sigma-Aldrich, Merck, Saint-Quentin Fallavier, France). Membranes were exposed to the appropriate peroxidase-conjugated secondary antibodies (1:5000 goat anti-guineapig IgG A108P or 1:10000 sheep anti-mouse IgG A5906 ...
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bioRxiv - Microbiology 2024Quote: ... Purification and concentration of □ ERS-1 was carried out with Amicon® Ultra-15 centrifugal filter with Ultracel100 kDa membrane (Merck Millipore) (Sun et al. ...
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bioRxiv - Genetics 2024Quote: ... The cells were first arrested at G1 using 3.0 µg mL-1 α-factor grown at 25°C (Merck, Cat. No. T6901). After 90 min ...
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bioRxiv - Plant Biology 2024Quote: Visualization of intracellular and apoplastic ROS in guard cells was performed using 20 µM H2DCFDA or 100 µM peroxy orange 1 for H2O2 (PO1; Merck Sigma-Aldrich), and 100 µg/ml Oxyburst Green H2HFF-BSA (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... The stock solution was added at 1% v/v to Plasmax cell culture medium (CancerTools cat# 156371) supplemented with 5% FBS (Merck cat# F7524) and penicillin/streptomycin (Gibco cat# 15070063) ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were resuspended in 1 mL PBS containing protease inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail; Merck, #11836153001) (PBSI ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: For GC-MS analysis all the samples were derivatized with N,O- Bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane (BSTFA) purchased from Merck (Darmstadt, Germany).
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bioRxiv - Biophysics 2024Quote: ... The membrane pellet was resuspended in extraction buffer (25mM MOPS-NaOH, 150mM NaCl, 50mM EDTA, 10mM DTT, 0.1 mg/ml lysozyme, 1% lauroylsarcosine (Merck, Cat. No. L5777), pH 7.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The extracts were prepared by incubating the formulations (1 ml, prepared as detailed above) in 3.3 ml DMEM HG medium (Merck, Cat. No. D6429) for 24 h at 37°C at a surface-to-volume ratio of 3 cm2/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... Chromatin was then sheared either by passaging 10 times through a 27G needle attached to a 1 ml syringe or by the addition of Benzonase® Nuclease (Merck, UK). Needle-sheared nuclear fractions were centrifuged at 13,000 x g for 10 minutes (4°C ...
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bioRxiv - Cell Biology 2024Quote: ... HaCaT KCs were cultured for 4 hours after seeding in DMEM supplemented with 10% FBS (HyClone) and 1× antibiotic–antimycotic mixed stock solution (Nacalai Tesque) on 3.0-µm-pored cell culture inserts (Merck KGaA, Darmstadt, Germany). The inserts were pre-coated with CELLstart (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The extracted 212Pb in 0.1 M HCl obtained from the emanation generator was adjusted to pH 5-6 with 5 M sodium acetate (Merck, Darmstadt, Germany) and mixed with TCMC-mAbs with specific activities of 1-50 MBq/mg ...
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bioRxiv - Cell Biology 2020Quote: ... Beads were then washed 2x in Tween wash buffer (5 mM Tris-HCl [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck], 0.05 % Tween-20 [Sigma]) and 1x in H2O ...
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bioRxiv - Microbiology 2021Quote: ... the cell layers were washed again with PBS and then lysed for 10 min with PBS containing 1% Elugent (Merck Millipore, Darmstadt, Germany) and 0,0625% Antifoam B (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2020Quote: About 8,000 T4 seeds from the W29-1-4 reporter line were mutagenized by treatment with 0.3% (v/v) ethylmethanesulfonate (Merck KGaA, Frankfurter, Darmstadt, Germany) for 15 h at 25°C ...
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bioRxiv - Bioengineering 2020Quote: Fibrinogen stock solutions were prepared by dissolving 1 g of human fibrinogen lyophilized from 20 mM citrate pH 7.4 solutions (Merck 341576, >90% clottable proteins) into distilled water as recommended by the manufacturer (final volume of app ...
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bioRxiv - Biochemistry 2020Quote: ... The imidazole of the elution fraction was removed until the concentration of imidazole was below 1 mM using Amicon® centrifugal filters (0.5 ml size, GlnA1: 30 kDa and GlnK1: 10 kDa) (Merck KGaA, Darmstadt, Germany). His6-sP26 was purified as described above with the following additional steps ...
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bioRxiv - Biophysics 2020Quote: ... functionalized with either 100 μg/mL poly-D-lysine overnight for microglia cells or with 0.2 mg/mL fibronectin (FC010, Merck, 1:5 in PBS) for 2h at 37°C for fibroblasts ...
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bioRxiv - Synthetic Biology 2021Quote: ... the diet for both the transgenic and wild-type strains was supplemented with CuSO4•5H2O (1 mM, Merck, CAS-no: 7758-99-9). Flies were reared in a controlled environment room at 25.0 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Free floating brain sections and fixed astrocyte cultures were immunostained with the following antibodies (Table S2): Mouse anti-NeuN (1:100, Merck Millipore, Darmstadt, Germany), Rabbit anti-GFAP (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Biophysics 2022Quote: ... was washed three times in Tris-buffer (pH 7.48) and resuspended in 1 %w/v Pluronic® F-127 (Merck Chemicals GmbH, Germany) Tris-Buffer.
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bioRxiv - Microbiology 2021Quote: ... aspergillus minimal media (AMM) [33] were added in pre-wetted 96-well MultiScreen Filter Plates HTS (1 μm glass fiber filter, Merck Millipore, Darmstadt, Germany) and incubated for 15 min at 37°C with either 25 μL PBS or 25 μL [Fe]TAFC solution (control – uptake block ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in blocking solution (5% bovine serum albumin in PBST) for 60 min at room temperature and then incubated with anti-CB1R (Merck Millipore, 1:1000) in blocking solution overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C followed by incubation with primary antibody: anti-tyrosine hydroxylase (TH, 1:1000, Merck Millipore, Darmstadt, Germany, AB152). After washing with TBST ...
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bioRxiv - Cancer Biology 2021Quote: ... PDGCLs were lentivirally transduced with the MISSION® shRNA vector pLKO.1-puro-CMV-Turbo green fluorescent protein (TurboGFP)_shnon-target (#SHC016, Sigma, part of Merck, Darmstadt, Germany) for cytosolic TurboGFP expression ...
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bioRxiv - Biochemistry 2022Quote: ... was added and incubated for 1 min at room temperature with gentle shaking (100 mM stock was prepared from fresh H2O2 Merck 30% w/w).
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bioRxiv - Biochemistry 2022Quote: ... the resulting solids were resuspended in 1 mL of UP-water and desalted using centrifugal filtration (Merck, Amicon® Ultra 10 kDa filters) at 14,000 rcf for 10 minutes at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Testes were washed three times (10 min each) with PBS-T and incubated with anti-H4ac (Merck Millipore #06-598, 1/500 dilution), anti-Histones (Millipore #MABE71 ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were blocked in 5% BSA TBS-T or 5% skim milk TBS-T and incubated overnight at 4°C with the following primary antibodies diluted in 5 % BSA or 5% skim milk dissolved in TBS-T: pSer293-PDH (1:1,000) (Merck Millipore, Cat. no. ABS204), PDH (1:1,000 ...