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Citations for Merck :
3451 - 3500 of 4003 citations for Propane 1 2 Diyl Diacetate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Fixed cells were washed twice with cytoskeleton buffer and stained with 1:500 30972 Abberior® STAR 635 (Merck) O/N at 4°C ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-mouse IgG HRP antibody (AP192P, Merck Millipore). Protein detection was performed using SuperSignal West Pico PLUS (34579 ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with a 1:5000 dilution of a donkey anti-rabbit IgG HRP antibody (AP182P, Merck Millipore) and 1:5000 dilution of mouse anti-GAPDH (ab8245 ...
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bioRxiv - Microbiology 2020Quote: ... NO2- concentrations were determined using colorimetric test strips (NO2--test, 0-10 mgNO2-N·L-1, Merck KGaA, Darmstadt, Germany).
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bioRxiv - Developmental Biology 2021Quote: ... We used the following primary antibodies: rabbit antibody against Protein Kinase Cα diluted at 1:500 (Antibody Registry ID: AB_477345, Merck), rabbit antibody against Recoverin at 1:1000 (AB_2253622 ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM K4Fe(CN)6 and 1 mg/ml X-gal (Roth)) and counterstained with nuclear fast red (Merck) for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... A 40 mg of lyophilized fecal sample was extracted with 1 ml of HPLC grade methanol (Merck, Mumbai, India) and kept in a shaker overnight at 1200 rpm ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated at 37°C for 1 hour with 10U/ml DNase (Benzonase, Merck-Millipore via Sigma-Aldrich) followed by centrifugation ...
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bioRxiv - Neuroscience 2023Quote: ... Neurons were plated (12-well plate) on glass coverslips coated with poly-L-lysin-hydrochloride (1 mg/ml, Merck). After 2h ...
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bioRxiv - Physiology 2023Quote: ... Mice were injected intravenously (i.v.) two consecutive days with 1 mg of biotin-X-NHS Ester (BXN) (Millipore, Merck) per mouse dissolved in 200 μl of saline buffer ...
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bioRxiv - Neuroscience 2023Quote: ... The reactions were incubated at 37 °C for 1 h and subsequently stopped by addition of 1x PhosStop (Merck). In vitro phosphorylation of dephosphorylated recombinant SYNJ2BP (0.5 µg in 10 µl ...
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bioRxiv - Cell Biology 2023Quote: ... Fabs were concentrated up to >1 mg/ml using 10 k cut-off filters (Amicon Ultra-0.5 10 k; Merck). Fabs were conjugated with Alexa Fluor 488 (Sulfodichlorophenol Ester ...
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bioRxiv - Molecular Biology 2023Quote: ... The input control was incubated in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with 40 mU/µL proteinase K (#P4850, Merck) for 2 h at 55°C and 1200 rpm ...
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bioRxiv - Biochemistry 2023Quote: ... HEK-293T cells were transfected at ∼40 % confluency using a 3:1 polyethylenimine (branched, average Mw ∼25,000 Da; Merck) to DNA ratio (30:10 μg ...
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bioRxiv - Biochemistry 2023Quote: ... membranes were blocked with 5% BSA in TSMT (20 mM Tris; 150 mM NaCl, Merck; 1 mM CaCl2 (Sigma); 2 mM MgCl2 ...
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bioRxiv - Genomics 2023Quote: ... Nuclei were then pelleted by centrifugation and lysed in a nuclei lysis buffer (50mM Tris HCl pH8.1, 150mM NaCl, 10mM EDTA, 1% SDS, Complete protease inhibitor cocktail (Merck)) for 20min ...
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bioRxiv - Immunology 2023Quote: ... These oligonucleotides were used to create a phage display library using the T7Select 415-1 Cloning Kit (Merck Millipore). Immunoprecipitation of the library was performed in accordance with a previously published protocol7,11 ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1% DDM, 10% (v/v) ethylene glycol, 0.005% asolectin (Avanti Polar Lipid, USA) and 0.005% CHAPS (Calbiochem, Merck, Germany), and eluted by an increasing proportion of the same buffer plus 1 M NaCl ...
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bioRxiv - Bioengineering 2023Quote: ... Ammonium persulphate ((NH4)2S2O8) 98.0% (APS) and glutaraldehyde (OHC(CH2)3CHO) (Grade 1, 25% in water) were obtained from Merck, Germany ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 24 h at +4°C with primary antibodies (goat anti-Chat (1:200, Merck, AB144P), mouse anti-GFAP (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... were diluted 1:400 v/v in 0.5% low gelling temperature agarose in Ames’ medium at 37°C (A1420; Merck). A thin film was polymerized over a pure agarose meniscus in a Petri dish and covered with Ames’ medium ...
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bioRxiv - Neuroscience 2022Quote: ... the second step consisted of incubating overnight in PBS-Triton with rabbit anti-Aldh1a1 (1:500, HPA002123, Sigma Merck) and goat anti-calbindin (1:500 ...
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bioRxiv - Molecular Biology 2022Quote: Cells were harvested and lysed in RIPA buffer (50 mM Tris/HCl pH 7.5, 150 mM NaCl, 1 % (v/v) Triton X-100 (Merck), 0.5 % (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... For the example images for survival assay neurons were incubated with chicken polyclonal anti-MAP2 (1:2000; Merck Milipore) and secondary antibody Alexa Fluor 488 ...
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bioRxiv - Bioengineering 2024Quote: ... THP-1 cells were treated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA, Merck Life Science NV) for 24 hours in a T75 culture flask ...
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bioRxiv - Cell Biology 2023Quote: ... protein thioesterase inhibitors (10 µM palmostatin and 0.2 mM HDSF (1-hexadecanesulfonyl fluoride)) and 250 U/ml Benzonase Nuclease (Merck). After 30 min (4°C) ...
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bioRxiv - Biochemistry 2024Quote: ... Diener Electronic) followed by channel surface functionalization using 1% (v/v) trichloro(1H,1H,2H,2H-perfluorooctyl) silane (Merck) in HFE-7500™ (3M™ Novec™).
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bioRxiv - Developmental Biology 2024Quote: mESCs were plated at a final density of 1 × 104 cells/cm2 on fibronectin-coated (MM Merck Millipore, FC010) µ-Slide 8 Well Glass Bottom Chamber Slides (Ibidi ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining of puromycin was performed by incubation with the anti-puro monoclonal antibody (1:1000, Clone MABE343, Merck) for 60 min at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were incubated o/n at 4°C in 10 ml washing buffer (1% [w/v] milk powder in TBS-T) containing a 1:10,000 dilution of anti-pan-ADP-ribose binding reagent MABE1016 (Merck) at 4°C48 ...
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bioRxiv - Microbiology 2024Quote: ... Whole cell samples were then analysed by immunoblot using anti-puromycin antibody (1:2000, anti-mouse, clone 12D10, Merck). As a loading control a duplicate SDS-PAGE was performed ...
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bioRxiv - Biochemistry 2024Quote: ... ROS quenching was carried out by treating cells with 5 mM N-acetyl cysteine (NAC) (616-91-1, Merck) for 1 h.
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (10,000 x g, 30 min, RT) and incubated for 1 hour with Ni-NTA His bind resin (Merck) rolling at RT ...
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bioRxiv - Biochemistry 2023Quote: ... coli pellet in 7.5 mL of TBS- N (Tris buffered saline with 0.1% NP-40) and supplemented with 1 x protease inhibitor cocktail tablet (Merck). Cells were sonicated for four rounds of one minute on ...
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bioRxiv - Developmental Biology 2024Quote: ... consisting of 250 μL of HTF and 1 mM reduced L-glutathione (GSH, Sigma-Aldrich-Merck KGaA, Darmstadt, Germany). To reduce the female to female variability ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... tyrosine-hydroxylase (TH) was detected with the primary antibody anti-TH in rabbit (1:600, EMD Millipore - Merck, USA). After washing in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by application of a Cy3-conjugated goat anti-mouse antibody (1:250 dilution, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Protein transfer was done using the Bio-Rad wet-blotting system at 500 mA for 1 h on 100% methanol-activated low-fluorescent Immobilon-FL membranes (0.45 μm pore size, Merck). The blot transfer buffer consisted of 25 mM Tris ...
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bioRxiv - Bioengineering 2023Quote: ... Medium was further concentrated to volumes <1 mL using 100kDa MWCO Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore). Tricorn 10/300 chromatography columns (Cytiva ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes were labeled before experiments with 1% of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (PE-Rhodamine) (Merck) at a concentration of 10µg/mL.
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bioRxiv - Cell Biology 2023Quote: ... including in an additional experiment where 1 mM of membrane permeable ATP (ATP disodium salt hydrate [A2383 – Merck, Australia]) was added in an attempt to rescue motility ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed three times with 1 x PBS and mounted using an antifade mounting media (Sigma-Aldrich/Merck). The images were captured using a Zeiss AXIO Observer.Z1 Inverted Fluorescence microscope (Zeiss) ...
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed 4 times for 5 min with 1 x PBS/Tween20 0.1 % (v/v) and the HRP conjugated secondary antibodies (A5420 and A4416, Merck) were added 1:5000 (goat ...
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bioRxiv - Microbiology 2023Quote: ... proteins were transferred on PVDF membranes and rabbit polyclonal antibodies were used at a 1:500 dilution (anti-Pic and anti-SepA) or a 1:250 dilution (anti-SigA) in 1X TBS supplemented with 0,5% of Tween 20 (Merck) and 3% BSA (Euromedex) ...
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bioRxiv - Biophysics 2023Quote: ... 5.5 µL of fibril sample was mixed with 0.5 µL of 1 mg/mL pronase protease (53702, Merck-Millipore) followed by adsorbing onto carbon-coated copper grids ...
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bioRxiv - Bioengineering 2024Quote: ... the THP-1 macrophages were detached from the MadSurface by incubating in 1mL/well Accutase (Merck Life Science NV) in the incubator for 1 h ...
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bioRxiv - Bioengineering 2024Quote: ... The cytoskeleton was stained using a 1:40 dilution of Alexa Fluor 488 conjugated phalloidin (Merck Life Science NV) in PBS and nuclei were stained using a 1:1000 dilution of DAPI (Merck Life Science NV) ...
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bioRxiv - Microbiology 2022Quote: Nasal sIgA was obtained from 1 ml nasal swab eluates by centrifugation (1 minute, 10 000 xg) and supernatant was sterile-filtered through a 0.4 μm pore (Merck). We determined sIgA concentrations by ELISA (Human IgA ELISA kit ...
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bioRxiv - Microbiology 2022Quote: ... and protein expression was induced O/N with 0.5 mM isopropyl 1-thio ß-D-galactopyranoside (IPTG) (Merck Chemicals). The next day ...