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Citations for Merck :
3451 - 3500 of 5322 citations for 6 9 Methano 4 1 benzoxazepin 2 3H one octahydro 3 methyl 3 α 5a bta 6 bta 9 bta 9a bta 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies used were rabbit anti-CB1R (1:1,000, Immunogenes) and mouse anti-actin (1:50,000, MAB1501, Merck Millipore). Primary antibodies were detected with horseradish peroxidase conjugated anti-rabbit and anti-mouse antibodies and visualized by enhanced chemiluminescence detection (Luminata Forte Western HRP substrate ...
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bioRxiv - Plant Biology 2022Quote: ... 250 µl of supernatant was added to 14.75 ml HNO3 (17.5%) containing 1 mg l-1 Yttrium (Merck Millipore) as an internal standard ...
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bioRxiv - Cell Biology 2022Quote: MCF10 DCIS.com (DCIS.com) lifeact-RFP cells (Riedl et al., 2008) were cultured in a 1:1 mix of DMEM (Merck) and F12 (Merck ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were then lysed in PK lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM EDTA, 150 mM NaCl, 1% Igepal-630 (Merck)) supplemented with 50 mM DTT and protease inhibitors (cOmplete™ protease inhibitor cocktail EDTA-free ...
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bioRxiv - Molecular Biology 2022Quote: ... Anti-K48-Specific Ubiquitin (05-1307, 1:1000) and anti-K63-Specific Ubiquitin (05-1313, 1:1000) from Merck-Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... Same volumes of the solvent phase were evaporated under nitrogen and lipids were resuspended in 100 µl of chloroform/methanol 1:1 (v:v) (Merck). Lipid extracts corresponding to 1 mg wet liver weight were applied onto HPTLC silica gel 60 plates (10 × 20 cm ...
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bioRxiv - Cell Biology 2021Quote: ... cultured in SF-900 II SFM at 27°C were transfected at a density of 0.4 × 106 ml-1 with 1 μg bacmid DNA using Excort IV Transfection Reagent (Merck) according to the manufacturer’s protocol ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... and incubated overnight in the same solution with the primary antibody to CB1R (1:1,000, rabbit, Immunogenes) and neuronal nuclei (NeuN) (1:1,000, mouse, MAB377, Merck Millipore), at 4 °C ...
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bioRxiv - Genetics 2022Quote: ... followed by overnight incubation at 4°C with primary antibodies (Anti-ASL, Abcam ab97370, 1:1000; Anti-GAPDH mouse, Abcam ab8245, 1:10,000; Anti-nitrotyrosine, Merck 05-233 ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mm NaF, 1 mm DTT, 0.1 mm Na3VO4, 1% Triton X-100, 0.1% SDS, and protease inhibitors; Merck, Millipore) and homogenized by sonification ...
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bioRxiv - Neuroscience 2024Quote: ... and then blocked for 1 h at RT with 0.2% Triton-X in PBS + 1% normal goat serum (NGS, Merck). Antibodies used and their concentration is found in Supplementary Table 4 ...
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bioRxiv - Genomics 2023Quote: ... Fungal biomass was then digested by adding 100 µL of 1 mg mL-1 Chitinase from Streptomyces griseus (Merck) and 200 µL of 50 mM EDTA and incubating at 37 °C for 3 hours ...
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bioRxiv - Microbiology 2023Quote: ... the lipids were resuspended in methanol:chloroform (1:1) and subjected to thin layer chromatography (TLC) using silica gel 60 plates (Merck). Total lipids were separated using chloroform:methanol:water (65:25:4 ...
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bioRxiv - Systems Biology 2023Quote: ... cells were washed twice with PBS and then cultured in a starvation medium composed of DMEM:F12 (1:1) supplemented with BSA (0.3% Sigma-Aldrich/Merck), L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... containing solid (7 g L−1 agar) full-strength Hoagland (2.75 mL well−1; pH adjusted to 6.5) (Sigma-Aldrich, Merck), sealed with parafilm and placed under the growth chamber ...
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bioRxiv - Biophysics 2024Quote: ... Total DNA was extracted by adding 1 mL of a 25:24:1 phenol:chloroform:isoamyl alcohol mixture (Merck, Darmstadt, Germany) along with 1 g of acid washed glass beads (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: A digestion buffer comprising of 0.05 mg/mL activated DNAse I (DNase I with HBSS in a 1:1 ratio, Merck), 10 mg/mL Collagenase (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... pLKO.1-puro-shRNACDK12 (TRCN0000196423 and TRCN0000368335) and pLKO.1-puro-shRNABRCA1 (TRCN0000244984 and TRCN0000244987) were purchased from Merck.
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bioRxiv - Plant Biology 2021Quote: ... A dilution series ranging from 1.25 μg ml−1 to 10 μg ml−1 bovine serum albumin (BSA, Merck KGaA) was used for calibration and processed together with the samples.
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bioRxiv - Cancer Biology 2021Quote: ... cells in IMDM+Glutamax medium were cryopreserved in liquid nitrogen until time of analysis complemented 1:1 with 80% FCS and 20% dimethyl sulfoxide (DMSO) (Merck).
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bioRxiv - Cell Biology 2020Quote: 1×106 wt and hem1-/- DCs were spun down and lysed in 50µl 1x lysis buffer (1ml 10x RIPA buffer - NEB, 9ml H2O, 1 tablet PhosStop and 1 tablet protease inhibitor mini - both Merck). Lysates were spun at full speed for 10min at 4°C and supernatants transferred to new tubes ...
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bioRxiv - Cell Biology 2020Quote: ... Mouse monoclonal antibodies directed against paxillin (# 5H11, IF dilution, 1:100) and talin (#TA205, IF dilution, 1:100) were from Merck Millipore ...
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bioRxiv - Cell Biology 2020Quote: ... product T7451; used at 1/500) and CHMP1B (rabbit polyclonal antibody, ATLAS product HPA061997; used at 1/500) were from Merck; CHMP2A (rabbit polyclonal ...
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bioRxiv - Developmental Biology 2021Quote: ... for 5 min at room temperature to loosen the zona pellucida and then treated with a hypotonic solution (1:1 mixture of 1.2% sodium citrate and 60% fetal bovine serum, FBS; Merck KGaA) for 10 min at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibodies, anti-rabbit IgG-HRP (1:5000, #7074, CST) or anti-mouse-HRP (1:10000, #12-349, Merck Millipore) in TBST containing 5% milk for 1 h at RT ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGLUT1, 1:1000, MAB5502, Merck) or rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were maintained under aseptic conditions in a 1:1 mixture of DMEM/F12 (DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Bioengineering 2021Quote: ... The LCLs were maintained under aseptic conditions in a 1:1 mixture of Dulbecco’s modified Eagle medium and nutrient mixture F-12 (DMEM/F12; DF-041-B; Merck Millipore) supplemented with 1% antibiotic/antifungal (15240062 ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated overnight at room temperature in darkness with the primary antibody solution containing mouse anti-vesicular Glutamate Transporter 1 (vGlut1, 1:1000, MAB5502, Merck) and rabbit anti-Glutamate Decarboxylase 65-67 (GAD 65-67 ...
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bioRxiv - Microbiology 2019Quote: ... Cultivations contained 1 mM L-tyrosine-13C9 (99% atom purity, Cortecnet) and 1 mM L-valine-13C5 (99% atom purity, Merck) and were conducted in four replicates at 37 °C and 180 rpm for 24 h ...
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bioRxiv - Biophysics 2022Quote: HEK293T cells were cultured in 10 cm tissue culture treated dishes grown at 37 °C in a 5% CO2 atmosphere in HEPES buffered DMEM/F12 1:1 (Merck) supplemented with 10% fetal bovine serum and 2 mM L-glutamine ...
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bioRxiv - Neuroscience 2019Quote: ... AB5543), chicken anti-β3 tubulin (1:500, AB9354) and mouse anti-ankyrin G (1:500, MABN466) were purchased from Merck Millipore ...
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bioRxiv - Molecular Biology 2021Quote: ... Secondary antibodies fused to HRP were used for detection (Goat anti-mouse HRP 1:3000, BioRad; Goat anti-rat HRP 1:3000, Merck Millipore ...
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bioRxiv - Genomics 2021Quote: ... The pellet was resuspended in PBS and the cells were then separated into three reactions: (1) added 1:20 volume of 1M NAI (03-310, Merck, 25 µl of NAI in 500 µl of infected cells ...
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bioRxiv - Immunology 2022Quote: Heparinised blood was diluted with PBS at a ratio of 1:1 and overlaid onto Histopaque 1083 (Sigma-Aldrich, Merck) density gradient medium using SepMate™-50 centrifugation tubes (Stem Cell Technologies) ...
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bioRxiv - Bioengineering 2022Quote: ... Disruption buffer needed to be extensively washed away before adding 1 ml 1 μM Atto590-NHS ester (NHS-Atto590, #79636, Merck) in PBS to each gel ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then placed in 10% horse serum/PB for 1 h before being incubated in primary goat anti-ChAT (1:500; #AB144P, Merck) for 2 d at 4°C to aid identification of CINs ...
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bioRxiv - Cell Biology 2024Quote: ... For super-resolution microscopy, samples were incubated with secondary antibodies (STAR635P (Abberior, 1:200) and Atto594 (Merck KgaA, 1:200)) ...
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bioRxiv - Immunology 2024Quote: ... and passaged two times on MDCK-II cells with FBS-deficient medium containing 1% penicillin/streptomycin and 1 µg/ml of N-tosyl-L-phenylalanine chloromethyl ketone-treated trypsin (TPCK-trypsin, Merck KGgA ...
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bioRxiv - Immunology 2023Quote: 200 μg of HLA.A2-peptide complex in PBS was concentrated to 1 mg ml−1 with the use of Amicon Ultra Centrifugal Filters (Merck), adjusted to pH 8.3 by adding freshly prepared NaHCO3 (0.1 M final concentration ...
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bioRxiv - Immunology 2023Quote: ... peptides were dissolved at a concentration of 1 to 5 mg ml-1 in 1x PBS and incubated with TCEP agarose CL4-B (Merck) for 1 hour to reduce paired cysteines ...
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bioRxiv - Molecular Biology 2023Quote: ... To deplete Xrn1 and Dcp2 with the AID tags (Nishimura et al., 2009; Morawska and Ulrich, 2013) 1-Naphthaleneacetic acid (1-NAA; N0640-25G, Merck) was added to a final concentration of 1 mM ...
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bioRxiv - Genetics 2023Quote: ... membranes were incubated with an anti-sera peptide based polyclonal rabbit GLK2-antibody (1:3,000) and a peroxidase-conjugated anti-rabbit antibody (1:10,000) (Merck, Darmstadt, Germany) for 1 h at room temperature in TBST [Tris-HCl 20 mM (pH 7.6) ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a rabbit anti-Kv4.3 primary antibody (1:10000, Alomone Labs) together with chicken (G)/mouse (L) anti-TH primary antibody (1:1000, Abcam / Merck) in a carrier solution (1% NGS ...
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bioRxiv - Cancer Biology 2023Quote: ... depending on manufacturer-suggested minimum seeding density) with lenti/retroviral supernatant in a 1:1 volumetric ratio and 8 µg/mL hexadimethrine bromide (Merck), before centrifugation at 900g for 30 min and returning to incubation at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2023Quote: ... washed in TBS-T and incubated with horseradish peroxidase–conjugated anti-rabbit (1:2000, #7074P2, Cell Signalling or 1:80000, #A0545, Merck) and anti-mouse (1:2000 ...
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bioRxiv - Plant Biology 2024Quote: ... Waters Inc.) was conditioned with 1 mL of 100% methanol and 1 mL of deionized water (Milli-Q, Merck Millipore). After the conditioning steps each sample was loaded on SPE column and flow-through fraction was collected with the elution fraction ...
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bioRxiv - Biochemistry 2024Quote: ... Nuclease reaction products were separated at a flow-rate of 1 ml*min-1 on a Chromolith Performance RP-18e 100 x 46 mm column (Merck) equipped with a guard cartridge ...
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bioRxiv - Microbiology 2020Quote: The crude ethylacetate extract of ALR-2 was purified via column chromatography using a LiChroprep RP-18 (15-25 μm) silica gel column (Merck, EMD Millipore Corporation, USA) and chloroform:methanol (ratio 4:1 ...
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bioRxiv - Biochemistry 2021Quote: ... modifying on previous reported conditions for the assay of MjRibK.[2] The reaction mixture was incubated at 37°C overnight and visualized using fluoresce-based thin layer chromatography (TLC) (Merck Silica gel 60 F254) using the long wavelength (365 nm ...