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Citations for Merck :
301 - 350 of 1429 citations for Recombinant Human GPC3 protein His tagged R PE labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... beads were removed and recombinant dynein was concentrated in a 100 KDa cut-off filter (Amicon Ultracel, Merck-Millipore) to a final volume of 500 μL ...
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bioRxiv - Microbiology 2023Quote: ... were incubated for 2 h at room temperature with the respective recombinant glycosyltransferase enzymes (6.3 µg/ mL) and UDP-GlcNAc (2 mM, Merck) in glycosylation buffer (15 mM HEPES ...
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bioRxiv - Cell Biology 2020Quote: Proteins from 3T3-L1 fibroblast lysates extracted with Cytobuster protein extraction reagent (Novagen®, Merck). After cell disruption for 10 min at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... protein lysates were cleared by centrifugation and protein concentration was measured using Coomassie reagent (Merck). Denatured ...
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bioRxiv - Bioengineering 2021Quote: Three sender area squares (3 × 3 mm) were put into a 24-well containing 300 μL of anti-His antibody (Merck, Germany, NOVG70796-3) (10 μg mL−1 in DPBS ...
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bioRxiv - Plant Biology 2021Quote: 2-AA-labeled N-glycans prepared for HILIC-HPLC were desalted on C18 ZipTips (Millipore, Merck, Warszawa, Poland), following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... cells pre-labeled with transferrin-Alexa Fluor 594 were treated for 2 hours with 10 μM SAR405 (Merck, cat ...
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bioRxiv - Molecular Biology 2022Quote: ... CMCs were incubated at room temperature for 4 hours with rhodamine-labeled phalloidin (1:100, Merck Life Science) and/or Alexa-Fluor-488 ...
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bioRxiv - Molecular Biology 2022Quote: ... sections were incubated at room temperature for 4 hours with rhodamine-labeled phalloidin (1:100, Merck Life Science) and/or Alexa-Fluor-488 or -568-conjugated IgG secondary antibodies (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Fractions containing monomeric and labeled 14.4.4 scFVs were concentrated with Amicon Ultra-4 centrifugal filters (10 kDa cut off, Merck) to arrive at a protein concentration between 0.2 mg ml-1 and 1 mg ml-1 ...
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bioRxiv - Molecular Biology 2023Quote: ... exponentially growing RPE-1 cells were pulse-labeled with 1 μM CldU (5-Chloro-2’-deoxyuridine, Merck, C6891) and 250 μM IdU (5-Iodo-2’-deoxyuridine ...
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bioRxiv - Microbiology 2021Quote: ... This solution was autoclaved once more and then filtered through a 0.2−μm Stericup Quick Release System PES filter (Merck) into clean sterile Schott glass flasks and stored at room temperature in dark ...
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bioRxiv - Cell Biology 2023Quote: ... The centrifuged medium was diluted with serum-free Opti-MEM supplemented with penicillin/streptomycin to a final concentration of 10% BGS and filtered using the Stericup-VP Sterile Vacuum Filtration System with a 0.1 µM PES filter (Merck). The cells were incubated with the medium for 48 hours ...
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bioRxiv - Biophysics 2023Quote: ... Liposomes were labeled before experiments with 1% of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (PE-Rhodamine) (Merck) at a concentration of 10µg/mL.
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bioRxiv - Immunology 2023Quote: ... RH5ΔNC-specific B cells were identified as live CD19+ IgG+ RH5ΔNC-APC+ RH5ΔNC-PE+ cells and single cell sorted into 96-well plates containing 10µL/well lysis buffer (10mM Tris [T3038, Merck] ...
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bioRxiv - Cancer Biology 2021Quote: Primary HPMECs in EBM2 medium or ST1.6R cells in DMEM/1% FBS were cultured overnight and stimulated with 2 μg/ml of recombinant TNC (Merck Millipore) for 24 h ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... The dose of COS (75-400 IU daily of recombinant follicle stimulating hormone (Gonal-F Merck or hMG Menopur, Ferring) was individualized to patient’s age ...
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Appropriate glycemic management protects the germline but not uterine environment in type 1 diabetesbioRxiv - Developmental Biology 2024Quote: ... Ovarian follicle growth tracking was performed by scheduled transvaginal ultrasound examinations that lead to the planning timepoint for oocyte maturation triggered by administration of recombinant hCG (250 microgr SC Ovitrelle; Merck). Oocyte retrieval was carried out by transvaginal ultrasographically-guided follicular puncture 37 hours later.
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bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Protein concentrations were estimated by the bicinchoninic acid (BCA) protein assay method (Sigma-Aldrich, Merck, USA). Proteins (50 μg ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
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bioRxiv - Cell Biology 2021Quote: Human immortalised articular chondrocyte C28/I2 cells were purchased from Merck Millipore and cultured in DMEM/F12 (Gibco ...
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bioRxiv - Cell Biology 2022Quote: The human brain microvascular endothelial cell line hCMEC/D3 (Merck Millipore) was seeded in T25 flasks coated with rat tail type I collagen (Merck Millipore) ...
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bioRxiv - Cancer Biology 2020Quote: ... When stained for human nuclei (MAB1281, Merck Millipore, Fig. 4C-D), this was performed for 30 min at RT in the dark after EdU staining (Click-iT Assay ...
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bioRxiv - Microbiology 2020Quote: ... and affinity-purified rabbit polyclonal antibody against human CPSF6 (HPA039973; Merck) were used at a dilution of 1:1,000 ...
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bioRxiv - Neuroscience 2022Quote: The HCYTOMAG-60K-15 Human Cytokine/Chemokine Multiplex Assay (Merck Millipore) was employed to simultaneously analyze TNF-α ...
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bioRxiv - Molecular Biology 2022Quote: ... 100 IU mL1 human chorionic gonadotropin (hCG; Chorulon; Merck Animal Health) and 1.0 mg mL1 oestradiol ...
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bioRxiv - Immunology 2023Quote: ... and human rIL-2 (30 U/ml) (Roche/Merck, Darmstadt, Germany) to the culture ...
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bioRxiv - Developmental Biology 2023Quote: ... Coating was performed with 20 µg/ml human fibronectin (Merck FC010) in PBS for at least 30’ ...
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Lowering mutant huntingtin by small molecules relieves Huntington’s disease symptoms and progressionbioRxiv - Molecular Biology 2023Quote: ... and anti-eIF4G1 for human samples (1:2000; 07-1800, Merck) or anti-eIF4G3 for mouse samples (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and antibodies directed against anti-human mitochondria (1:100, Merck #1273;), CDK2AP1 (rabbit ...
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bioRxiv - Bioengineering 2024Quote: ... anti-human nuclei (HuNu; 1:100; MAB4383, Merck Millipore, MA, USA), anti-Ku80 (1:100 ...
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bioRxiv - Biochemistry 2022Quote: ... Protein standards were obtained from Merck.
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... 2.0 Protein Detection System (Merck Millipore), incubated with Clarity Western ECL (BioRad) ...
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bioRxiv - Biophysics 2020Quote: ... fluorescently-labeled and biotinylated H57-scFV were again concentrated with 10kDa Amicon®Ultra-4 centrifugal filters (UFC801024, MERCK) and stored in PBS supplemented with 50% glycerol at −20°C ...
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bioRxiv - Bioengineering 2020Quote: ... labeled for 30 minutes at 4ºC and washed with PBA (PBS with 0.2% sodium azide [Merck]; 1% BSA [Sigma]).DAPI (final concentration of 1 μg/ml ...
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bioRxiv - Cell Biology 2023Quote: ... the TurboID-CpOGACD or TurboID-CpOGADM expressing HEK293T cells were labeled with 10 to 100 µM biotin (Merck, B4501) in the medium for 15 min to 3 h ...
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bioRxiv - Molecular Biology 2023Quote: ... Switzerland) and further processed by tangential flow filtration (30 kDa MWCO, PES membrane; Pellicon XL 50; Millipore, Merck, Darmstadt, Germany), autoclaved ...
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bioRxiv - Cell Biology 2023Quote: ... Media containing high titre pseudo virus was collected at 4 h intervals after the first media exchange and continuously for 48 h thereafter and filtered through a 0.45 μm PES filter (Merck, SLHP033RS) to remove cell debris ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: In situ protein-protein interaction was evidenced using Duolink® Proximity Ligation Assay (Merck, DUO920082, DUO92004, DUO92008). Cells were fixed with formaldehyde and permeabilized using ice-cold 90% methanol ...
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bioRxiv - Biochemistry 2020Quote: ... 500 μM ATP and ~0.5 μg commercially available human CyclinB-Cdk1 (Merck). The reaction was carried out at 30 °C and was stopped using SDS-PAGE sample buffer after 5 minutes.
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bioRxiv - Cell Biology 2019Quote: ... Human pancreatic organoids were additionally immuno-stained against Sox9 (primary antibody: Merck Millipore ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... a rabbit anti-human EGFR monoclonal antibody (EMD Millipore-MERCK, Temecula, USA) diluted 1:1000 or a rabbit anti-human VEGF-A polyclonal antibody (Abcam ...
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bioRxiv - Neuroscience 2020Quote: ... human tau constructs were expressed in the vector pNG2 (Merck-Novagen, Darmstadt) in E ...
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bioRxiv - Immunology 2021Quote: ... MILLIPLEX MAP Human High Sensitivity T Cell Panel (Merck Cat# HSTCMAG-28SK) were used ...
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Chromatin priming elements direct tissue-specific gene activity prior to hematopoietic specificationbioRxiv - Genomics 2023Quote: ... 501µg/ml Ascorbic acid and 180⍰µg/ml Human transferrin (Merck, T8158)) and then plated onto non-adherent dishes (ThermoFisher ...
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bioRxiv - Bioengineering 2023Quote: ... Immortalized human cerebral microvascular endothelial cell line (hCMEC/D3 Merck Life Science) were cultured on 250µg/ml collagen-I (Advanced Biomatrix ...
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bioRxiv - Developmental Biology 2023Quote: ... and reinjected after 48 hours 7.5IU hCG (human chorionic gonadotrophic hormone; Merck), before overnight mating with F1 or F1 hybrid transgenic mT/mG stud males (original mT/mG transgenic mouse line obtained from Jackson Laboratories ...
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bioRxiv - Biophysics 2023Quote: ... A solution was prepared by mixing 70 µL human plasma fibronectin (Merck), 20 µL green fibrinogen and 910 µL PBS ...