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Citations for Merck :
301 - 350 of 3092 citations for PD 1 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... rh-HI was prepared in either 20% acetic acid (v/v from acetic acid glacial, Merck KGaA, Darmstadt, Germany), 0.3 M NaCl (Merck KGaA ...
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bioRxiv - Cancer Biology 2023Quote: ... Cdk1 and Cdc25B cDNAs were subcloned in frame with the N-terminal His-tag into pRSETa (Novagen, Merck Biosciences). Cdc25B cDNA was also subcloned into pcDNA3 without (pCdc25B) ...
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bioRxiv - Neuroscience 2019Quote: ... before probing overnight at 4°C with primary antibodies (1:500 mouse anti-NF200 [N0142; Sigma-Aldrich] and 1:500 rabbit anti-peripherin (AB1530; Merck Millipore) in blocking solution ...
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bioRxiv - Neuroscience 2021Quote: The activation of Caspase 1 in mouse brain samples were determined using Caspase 1 Colorimetric Assay Kit (YVAD, Merck Millipore, Burlington, MA) and following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were also probed with either a mouse monoclonal antibody against GAPDH or a mouse monoclonal antibody against beta-actin (dilutions of 1:10,000 and 1:1000; Sigma-Aldrich/Merck group, USA) and with the IRDye® 680CW secondary antibody from Eurobio Ingen (926-32220 ...
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bioRxiv - Neuroscience 2020Quote: ... and mouse monoclonal IgG2A antibody to myelin Proteolipid Protein (clone PLPC1, MAB388, MERCK-Millipore, 1:100). All slides were mounted using Mowiol mounting medium (475904 ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated with 1:1,000 mouse anti-Rac1 (clone 23A8 05-389; Merck Millipore, USA) overnight at 4 °C and 3 h at RT and then washed 3 times with TTBS ...
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bioRxiv - Neuroscience 2023Quote: ... We used the primary antibody of anti-PV mouse monoclonal antibody (1:1000, MAB1572, Merck Millipore) combined with the secondary antibody of anti-Mouse polyclonal goat antibody conjugated with Alexa Fluor 647 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and mouse anti-human TMPRSS2 (monoclonal, clone P5H9-A3, cat num: MABF2158, 1:500, MERCK, USA) for a minimum of 2 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... the retinal sections were stained with different antibodies: mouse anti– rhodopsin 1D4 (1:1500, 1D4, Merck), rabbit anti-M-opsin (1:300 ...
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bioRxiv - Molecular Biology 2023Quote: ... and Alexa Fluor 488-conjugated goat anti-mouse antibody (diluted at 1:200, #AP124C; Merck Millipore) as the secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: mouse monoclonal antibody against actin (1:10,000, Merck Millipore, MAB1501R); mouse monoclonal antibody against PrP (POM1 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then first incubated with mouse anti-PSD95 antibodies (1:100; Merck-Millipore, Cat. #MAB1596) overnight at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... GAPDH (mouse; Merck), FLAG (mouse ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG (mouse; Merck), FLAG (rat ...
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bioRxiv - Neuroscience 2022Quote: ... FoxP2 (mouse, Merck Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 ug inactive MEK1 (C-terminally His-tagged) in a total volume of 40 μL buffer (20 mM Hepes pH 8 (MERCK, Sigma Aldrich ...
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bioRxiv - Molecular Biology 2022Quote: ... and applied directly onto a column of 2 ml pre-equilibrated Ni-NTA beads (cOmplete His-Tag Purification Resin from Merck). After several washes with high salt (500 mM NaCl) ...
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bioRxiv - Molecular Biology 2022Quote: ... the supernatant was loaded on to a gravity flow-based Ni-NTA metal affinity column (2 ml beads, cOmplete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt (50mM Tris pH8.5 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6-tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with Ni-NTA His•Bind® Resin (Merck). Fractions containing the protein of interest were then dialysed against low-salt buffer (50mM Tris pH7.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... WT—or its mutants fused with a His-tag on the amino-terminus—were expressed in Escherichia coli BL21 (DE3) using pET15b vector (Merck). A single colony was inoculated in 2 mL Luria-Bertani broth (LB ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... the supernatant was loaded onto a gravity flow-based Ni-NTA metal affinity column (2 ml beads, complete His-Tag Purification Resin from Merck), equilibrated and washed with 10 column volumes of buffer A containing 5 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... His-tagged Trx1 was purified on a Ni2+ sepharose resin following the manufactureŕs instructions (cOmplete™ His-Tag Purification Resin, Merck).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The supernatant was passed through a 0.45 µM syringe filter before transferring into a tube containing HIS-Select Nickel Affinity Gel (Merck India) and kept on a mixer at 4°C for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
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bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
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bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated (1 hour, 1/5000 dilution, RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
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bioRxiv - Microbiology 2023Quote: ... the cells were stained with goat anti-rabbit Alexa Fluor® 555 (Thermo, 1:1,000) and mouse anti-IE Alexa Fluor® 488 (Merck, 1: 1,000) for 1 hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
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bioRxiv - Cell Biology 2021Quote: Primary antibodies used in this study were: mouse monoclonal antibodies against Centrin (1:500 for immunofluorescence; Merck Millipore catalogue number 04-1624 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies used were: monoclonal mouse anti-puromycin (clone 12D10, MABE343, Merck Millipore; 1:1000), polyclonal rabbit anti-Tau (T6402 ...
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bioRxiv - Cell Biology 2020Quote: ... c-Src was detected using anti-Src (GD11 clone) antibody (1:200, Merck Millipore, Mouse, 05-184). Phosphorylated c-Src was assessed using anti-phospho-Src Tyr 418 antibody (1:100 ...
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bioRxiv - Genomics 2022Quote: ... firstly with anti-DIG2 (sheep anti-mouse Ig digoxigenin conjugate, 1:200, Merck Millipore, Billerica, MA, USA) and antistreptavidin (1:25 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with primary antibodies against puromycin (mouse monoclonal 1:500, Merck), Par3 (rabbit polyclonal 1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated overnight at 4 °C with an anti-puromycin antibody (mouse monoclonal 1:500, Merck) combined with an anti-β-actin antibody (rabbit polyclonal 1:1000 ...
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bioRxiv - Biophysics 2021Quote: ... Cells were then incubated for 1 h at room temperature with the monoclonal mouse Anti-Tom20 (1:500, MABT166, Merck & Co., Kenilworth, NJ, USA). The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500 ...
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bioRxiv - Biochemistry 2022Quote: ... The His6- tagged proteins were purified using Immobilised Metal Affinity Chromatography (IMAC) with 2mL Ni-NTA His•Bind® Resin (Merck), followed by incubation with TEV protease for 12 hours at 4 °C to remove the His6 tag ...
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bioRxiv - Immunology 2020Quote: ... The elimination of the N-terminal His tag was performed through the cleavage with biotinylated thrombin included in the Thrombin Cleavage Capture Kit (Merck, Millipore) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Recombinant full length SIC proteins were purified using affinity chromatography with a His-bind column as per manufacturer’s instructions (Novagen, Merck, Darmstadt, Germany). sic1.300 ...
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bioRxiv - Biochemistry 2022Quote: ... The His-tag-free Mpro in the flow-through was concentrated by using Amicon Ultra 15 centrifugal filters (10 kD, Merck Millipore) at 2773 x g ...
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bioRxiv - Molecular Biology 2020Quote: ... mouse anti-GAPDH (Merck), and rabbit anti-actin (Santa Cruz ...
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bioRxiv - Biochemistry 2020Quote: ... and mouse monoclonal anti-APP (1:500, 22C11; Cat#Mab348, RRID: AB_94882, Merck Millipore/Sigma, Burlington, MA, USA) antibodies were purchased from the indicated suppliers ...
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bioRxiv - Neuroscience 2021Quote: ... brain slices of mice were also incubated with a mouse NeuN (1:500, conjugated with AF488 MAB377X, Merck) and a rabbit GFAP antibody (1:1000 ab7260 ...
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bioRxiv - Neuroscience 2020Quote: ... neuronal somata were visualized by NeuN immunolabeling (1:1000, monoclonal mouse anti-NeuN IgG, #MAB377, /Merck, Darmstadt, Germany) in CRH-ires-cre ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were incubated overnight at 4°C with monoclonal anti-NeuN antibody (1:500; Mouse, MAB377, Merck Millipore), in a 50% dilution of blocking buffer + 0.5% Triton X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... The following primary antibodies were used: anti-APC mouse monoclonal antibody (1:200; Merck Millipore, Burlington, MA, USA), anti-GFAP-488 mouse monoclonal antibody (1:300 ...