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Citations for Merck :
301 - 350 of 2432 citations for Nuclear pore complex protein Nup93 dye Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 4 °C) and filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Ni-NTA agarose (1.3 ml resin/ 1 l insect cell pellet ...
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bioRxiv - Synthetic Biology 2020Quote: ... proteins were electrotransferred during a 30-min interval from the gel onto an Immobilon-P membrane of pore size of 0.45 µm (Merck Millipore, USA) under constant electric current of 0.1 A per gel and voltage of 5-7 V using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... After centrifugation for 10 min at 5,000 rpm the supernatant was collected and filter-sterilized using a 0.22 µm pore size hydrophilic polyethersulfone filter (Merck Millipore, Germany). The cell-free supernatant was then ultracentrifuged at 150,000 x g for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... and the remaining sample was centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Microbiology 2021Quote: ... they were centrifuged at 10000 g for 5 min and filtered through a 0.2 μm pore Millipore filter (Merck, KGaA, Germany) previously wetted with 1 ml of 0.9% NaCl solution ...
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bioRxiv - Bioengineering 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA) a constant flow rate of 0.3 ml/min with mobile phase A being 0.1 % Formic acid in 99:1 water:acetonitrile (Honeywell ...
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bioRxiv - Cell Biology 2023Quote: Tumor slice and ear derma explants were then cultured on a 30-mm cell culture insert featuring a hydrophilic PTFE membrane (0.4 µm pore size, Merck Millipore #PICM0RG50) placed inside 6-well plates containing 1.1 mL of RPMI ...
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bioRxiv - Cell Biology 2022Quote: ... and subsequently filtrated using membrane filters with a pore size of 0.45 μm (Millex® HV membrane filter units, Merck Millipore). The clarified lysate was supplemented with Glutathione Sepharose 4B (Cytiva ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml/min ...
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bioRxiv - Microbiology 2024Quote: ... All but one sample for microbiological analyses was collected onto 0.22 μm pore size filters (type GVWP; Merck Millipore, Darmstadt, Germany) held in pressure-resistant stainless steel filter holders directly connected to the tubing outlet under in-situ hydraulic pressure conditions ...
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bioRxiv - Microbiology 2024Quote: ... This suspension was diluted with 9 mL of 0.9 % NaCl and filtered through a hydrophilic polycarbonate filter (pore size 0.2 µm; Merck Millipore, Darmstadt, Germany). A segment from the center of the filter was fixed with 1 % glutaraldehyde and 4 % paraformaldehyde in washing buffer (10 mM cacodylate buffer ...
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bioRxiv - Cell Biology 2023Quote: ... was performed using aluminum plates pre-coated with silica gel (0.25-mm, 60-Å pore size, 230−400 mesh, Merck KGA) impregnated with a fluorescent indicator (254 nm) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the extracted liquid sample was collected by centrifugation through a filtered microcentrifuge tube (cellulose acetate membrane with pore size 0.45 μm; Merck CLS-8162), (x ...
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bioRxiv - Synthetic Biology 2023Quote: ... 3.5 μm particle size, 100 Å pore size) connected to a ZicHILIC guard column (20 × 2.1 mm, 5 μm particle size) (Merck KgAA), with a constant flow rate of 0.3 ml min−1 ...
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bioRxiv - Microbiology 2022Quote: Parasites were mechanically released from host cells using a 27-gauge needle and passed through a Millex filter unit (5 μm pore size, Merck Millipore) to remove host cell debris ...
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bioRxiv - Microbiology 2022Quote: ... Cell-free supernatant (CFS) was prepared by filtering the spent media through a membrane filter with a 0.22 μm pore size (Merck Millipore, USA). MColorpHast pH indicator strip (Millipore ...
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bioRxiv - Microbiology 2023Quote: MDCK cells (2 x 105 cells) were cultured on polycarbonate Millicell culture plate inserts (12- mm diameter, 3-µm pore size; Merck Millipore) at 37°C under 5% CO2 atmosphere.
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bioRxiv - Immunology 2024Quote: ... the slices were cultured for 4-6 days onto culture membranes (uncoated 30 mm Millicell-CM tissue culture inserts with 0.4 mm pores, Merck Millipore, Germany) and kept in six-well dishes (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was immediately separated from the cells by filtration through a 0.45-µm pore size filter (PVD membrane, Merck Millipore Ltd.) ((adapted from [59]).
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bioRxiv - Genetics 2021Quote: ... Prior to EMSAs the in vitro translations of Rbpj (WT) and mutant proteins were monitored by western blotting using an anti-Flag antibody (M5, Merck).
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bioRxiv - Molecular Biology 2021Quote: ... The blots were incubated with primary antibodies to the following proteins overnight at 4 °C: (1) CTCF (1:1,000; 07-729, Merck Millipore), (2 ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was incubated with secondary antibody for 1 hour at room temperature and proteins were detected using chemiluminescent HRP substrate (Merck-Millipore ...
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bioRxiv - Microbiology 2022Quote: ... The clear cell-free extracts were immunoprecipitated using monoclonal antibodies against T18 tag following Protein G Immunoprecipitation Kit (Merck Inc) protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Cell Biology 2023Quote: ... The amount of puromycin incorporated into nascent peptides was then evaluated by Western blot analysis on 20 μg of proteins using anti-puromycin antibody (ref MABE343) purchased from Merck Millipore (Darmstadt ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed as previously described7 using Protein G Dynabeads (LifeTechnologies) coupled to Myc 4A6 antibody (Merck Millipore, 05-724) or FLAG M2 monoclonal antibody (Sigma ...
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bioRxiv - Plant Biology 2024Quote: ... Phosphorylated SR proteins were detected using a mouse monoclonal α-pan-phosphoepitope SR-specific antibody (1H4, Merck, Catalogue-No.: MABE50) at 0.025 µg/mL ...
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bioRxiv - Plant Biology 2024Quote: ... Protein samples were separated on 12% non-reducing SDS-PAGE gel and cysteine-dimedone protein adducts visualised by western blot analysis using 1:10,000 diluted rabbit anti-cysteine sulfenic acid primary antibody (Merck Millipore) and 1:10,000 diluted horseradish peroxidase conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... in Biotin binding buffer (5 mM Tris-HCl pH 7.5 [Sigma], 0.5 mM EDTA [AppliChem], 1 M NaCl [Merck]) for 1 h at RT ...
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bioRxiv - Biophysics 2020Quote: ... Refolded I-Ek/MCC(ANP)-biotin complexes were purified via 14.4.4S mAb-based affinity chromatography (Cyanogen bromide-activated Agarose, MERCK) followed by gel filtration (Superdex-200 10/300 GE Healthcare Life Sciences) ...
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bioRxiv - Molecular Biology 2024Quote: ... The bound complexes were eluted in lysis buffer 2 containing 25 mM glutathione or 5 mM biotin (Merck), respectively ...
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bioRxiv - Immunology 2023Quote: Immunoblotting was performed as described above and MIF-6xHis-biotin revealed using streptavidin-HRP-conjugate (Merck, Darmstadt, Germany), while MDL1-6xHis was revealed using a custom-made monoclonal primary antibody (1:10 dilution) ...
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bioRxiv - Cell Biology 2022Quote: ... The mitochondria were stained in vital cultures with MitoRed dye (Sigma-Aldrich/Merck, Poznan, Poland) prepared in CGMEPC (1:1000) ...
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bioRxiv - Microbiology 2023Quote: ... aeruginosa CE was measured using the fluorescent dye PI as per manufacturer’s instructions (Merck, UK). Late-exponential phase cultures of P ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... incubated 1 min in cresyl violet dye (1% of powder in absolute ethanol, Merck Millipore), then held 5 sec in 90% ethanol ...
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bioRxiv - Neuroscience 2024Quote: ... slices were incubated for 3 min with Hoechst 33342 dye (Merck Millipore, Billerica, MA, USA) (2µg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... The excess dye was removed using NaCl (+) buffer with Microcon 300 centrifugal filter units (Merck) according to the manufacturer’s protocol four times ...
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bioRxiv - Plant Biology 2024Quote: ... Following a 15 min incubation with 0.05% (w/v) Evans blue dye (Merck, Darmstadt, Germany), unbound dye was removed by thorough washing with H2O ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added to the mix followed by incubation at room temperature for 2 h ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Protein G Plus/Protein A Agarose beads (Merck Millipore #IP05) were added and incubated at room temperature for 2 hours ...
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bioRxiv - Microbiology 2021Quote: ... biofilm samples were rinsed with deionized water and deposited on polycarbonate filters (GTTP Isopore membrane filters, Merck Millipore, pore size 0.2 μm) or on glass slides (for correlative Raman analyses ...
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bioRxiv - Microbiology 2021Quote: ... groundwater was pumped directly into a sterile chilled Nalgene filtration unit fitted with a 0.22 µm pore size Isopore polycarbonate membrane (Merck Millipore, Darmstadt, Germany). Approximately 10 L of groundwater was filtered each for metagenomic and metaproteomic sample and 1 L was filtered when samples were taken for 16S rRNA gene amplicon analysis only ...
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bioRxiv - Microbiology 2021Quote: ... approximately 50 - 100 L of groundwater was filtered sequentially through 0.2 µm and 0.1 µm pore sized PTFE filters (142 mm, Omnipore Membrane, Merck Millipore, Germany; Table S1). With the exception of H32 (did not yield sufficient volumes) ...
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bioRxiv - Cell Biology 2021Quote: ... 5 × 105 cells were seeded on the upper chamber of 24 well plate cell culture inserts containing 3 μm pores (Merck, Cat # 353096) coated with rat-tail collagen I (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 0.1 mL of fixed cell suspension was filtrated by 0.22-µm pore-sized black polycarbonate filter (type GTBP; Merck Millipore, Darmstadt, Germany) and stained with 10 × SYBR Green I (SYBR® Green I Nucleic Acid Stain ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell viability was assessed by trypan blue exclusion and 3 × 106 viable cells from each condition were loaded into the upper chambers of Transwell culture inserts with a 0.5 µm pore size (Merck, Burlington, MA, USA). Cells were allowed to migrate towards the lower well containing medium ...
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bioRxiv - Microbiology 2021Quote: ... the supernatant was harvested by centrifugation at 10,000 x g at 4°C for 30 min and filter-sterilized using a 0.45 μm pore size hydrophilic PVDF membrane (Millex®-HV 0.45 μm, Merck Millipore, Darmstadt, Germany).
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bioRxiv - Biochemistry 2021Quote: ... free radioligands bound to DCC were removed using a vacuum filtration system with a 96-well filtration plate (MultiScreenHTS HV, 0.45-mm pore size, Merck KGaA, Darmstadt, Germany) for the bound/free (B/F ...
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bioRxiv - Cell Biology 2021Quote: ... and transferred to Immobilon 0.20 μm (LC3, GAPDH) or 0.45 μm (CD9, CD63) pore-sized PVDF membranes (Merck Millipore Ltd., Cork, Ireland) by wet transfer ...
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bioRxiv - Cell Biology 2021Quote: ... Retinal pieces were cultured on a cell culture insert with a 0.4 µm pore diameter (Millicell PICM0RG50, Merck Millipore Ltd, Cork, Ireland) in DMEM/Ham’s F-12 without Phenol Red (Nacalai Tesque ...