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Citations for Merck :
301 - 350 of 402 citations for Factor B Bb Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: TEER measurements [22] were performed on 3rd day of incubation with growth factors and (or) different inhibitors by using Millicell ERS-2 Electrical Resistance System (Merck-Millipore). Each insert was measured in three different locations ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine quantification was performed using a Human Cytokine/Chemokine/Growth Factor Panel A 48-Plex Premixed Magnetic Bead Multiplex Assay (Merck Millipore), using the Luminex MAGPIX System in 96-well plate format ...
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bioRxiv - Developmental Biology 2023Quote: ... 80% of complete medium was changed to either new complete medium without trophic factors or complete medium containing 5 ng/mL BDNF (GF029 Merck Millipore) or 100 ng/mL human PGRN (AG-40A-0188Y Adipogen) ...
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bioRxiv - Cell Biology 2021Quote: ... The floated sections were rinsed briefly with 100 mM maleate buffer (pH 7.5) and blocked for 30 minutes against non-specific antibody binding with 2% blocking reagent (Merck) in maleate buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were then permeabilized in blocking solution containing 5% normal goat serum (NGS, Sigma-Aldrich, Merck KGaA, Darmstadt, Germany) in 0.5% Triton for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... were incubated for 24 hours in blocking buffer containing 10% (vol/vol) normal donkey serum (NDS) (S30-100ML, Merck), 0.5% (vol/vol ...
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bioRxiv - Cell Biology 2022Quote: ... The buffers used during the whole procedure had fish gelatine 1.6% (v/v) as a blocking agent (G7765, Merck). The primary antibodies used were anti-NR1D1 (ab174309 ...
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bioRxiv - Neuroscience 2023Quote: ... in samples where biotin-streptavidin amplification was used before blocking with 10% chemiblocker (Merck Millipore, Billerica, MA, USA, #2170) for 2 hours at room temperature ...
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bioRxiv - Neuroscience 2024Quote: Whole iPSC-brain organoids were permeabilized with 0.5% Triton X-100 in blocking solution with 2% Bovine Serum Albumin (BSA; Merck) in PBS for 3 hours at RT ...
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bioRxiv - Neuroscience 2024Quote: ... All primary and secondary antibodies were diluted in a blocking solution consisting of PBS and 0.2% (v/v) bovine serum albumin (BSA, Merck). Cells were first washed with ice-cold PBS and incubated for 5 min in a blocking solution on ice ...
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bioRxiv - Plant Biology 2020Quote: ... the strain was grown on solid King’s medium B (Pseudomonas agar F, Merck KGaA, 64271 Darmstadt, Germany) supplemented with 50 μg mL−1 rifampicin at 25°C in the dark for 3 days ...
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bioRxiv - Synthetic Biology 2021Quote: ... with Hygromycin B resistance used as the selection marker as previously described.[49] Spermidine (Merck Life Sciences) was used to precipitate DNA onto gold particles of 0.3-3µm diameter (ChemPur) ...
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bioRxiv - Biophysics 2020Quote: ... Then His-tag containing OpuAC was introduced to the flow cell (in buffer B supplemented with 10 mM of (±)6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox; Merck) as a photostabilizer[20] ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were grown for 48h in serum-free ESGRO Complete Clonal Grade Medium (Merck, Cat# SF001- B) supplemented with 1000 U/ml LIF ...
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bioRxiv - Cell Biology 2022Quote: ... at a final concentration of 5 μg/ml in M2 medium or Latrunculin B (428020-1MG, Merck) at a final concentration of 5 μM in M2 medium (to disrupt F-actin) ...
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bioRxiv - Immunology 2023Quote: ... and interacting infected cell-invaded B cell doublet images acquired with ImageStream X MKII (Amnis/Merck Millipore) were analyzed by IDEAS 6.3 (Amnis/Merck Millipore/Luminex).
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bioRxiv - Plant Biology 2021Quote: ... Protein complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2021Quote: Peptides EcFtsN1-32-C and VmFtsN1-29-C were labelled with maleimide-Atto 495 (Merck Life Science UK). EcFtsA1-405 and VmFtsA1-396 were buffer exchanged into binding buffer (50 mM HEPES/KOH ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were dissolved in 2% acetonitrile containing 0.1% trifluoroacetic acid and desalted using C18 ZipTips (Merck Millipore, Germany). Each sample was independently analysed on a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were diluted to 50 µg/mL in 10 mM sodium acetate pH 5 (Merck, Darmstadt, Germany) and injected overflow cell two of each channel to a final signal of 3400 RU for L-CDP1 and 1300 RU for L-CDP7 ...
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bioRxiv - Microbiology 2024Quote: ... and subsequently eluted in 150 μL of wash buffer with 500 ng/μL FLAG peptide (Merck, Darmstadt, Germany) by rotation on a rotator for 2 h at 4 °C ...
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bioRxiv - Synthetic Biology 2022Quote: ... the supernatant was sterile filtered (0.22 μm) and afterwards concentrated by the factor of 20 using 100 kDa molecular weight cut-off filters (Merck KGaA, Darmstadt, Germany). To determine the number of released vesicles ...
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bioRxiv - Immunology 2020Quote: ... The sections were blocked in PBS containing 1% bovine serum albumin (BSA) for 1 h at RT and stained in blocking buffer containing primary antibody (anti-PP6C, Merck Millipore cat ...
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bioRxiv - Developmental Biology 2021Quote: ... They were incubated 1h at RT in a blocking buffer (20% BR + 20% goat serum) and then overnight with an anti-DIG-AP antibody (1:2000, Merck) in the blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... MDMi cells were incubated with a primary antibody against Iba1 (1:1,000; 019-19744, FUJIFILM Wako Chemicals) in blocking buffer (2% bovine serum albumin, 0.1% TritonX100 (Merck, Darmstadt, Germany), and 5% normal donkey serum (017-000-121 ...
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bioRxiv - Neuroscience 2023Quote: ... this was followed by the incubation in the blocking solution containing secondary antibody anti-Rabbit or/and goat IgG antibody conjugated with HRP (GENA934, AP106P, Merck) at room temperature for 1 hour for enhanced chemiluminescence ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were caught and rinsed in 1x PBS (3 × 15 min) prior to incubation in blocking solution (5% NDS (Merck) in 0.3% PBS-Triton-X-100 ...
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bioRxiv - Neuroscience 2023Quote: ... brain sections were washed three times for 15 min with 1X TBS and then incubated for 1.5 h in blocking solution: 2.5% Donkey Serum (Sigma, Merck, Germany, S30); 2.5% Goat Serum (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... washed with buffer B to stop the spheroplasting reaction and then washed into 10 % formamide (Merck Millipore S4117) in 2× SSC.
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bioRxiv - Physiology 2020Quote: ... all the birds were challenged with the Coccivac B-52 live vaccine (Merck Animal Health; 1.3× recommended dose). The vaccination was completed at d 5 to facilitate uniform intake of coccidian oocysts by the birds ...
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bioRxiv - Plant Biology 2019Quote: ... 0.8 % phytoagar (Duchefa) and 10 µg mL-1 Glufosinate-ammonium or 25 µg mL-1 hygromycin B (Merck) for herbicide selection ...
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bioRxiv - Genomics 2021Quote: ... with A pestle ~10 times following B pestle ~20 times in lysis buffer [5 mM CaCl2 (Merck, A546282), 3 mM Mg(Ac)2 (Roth ...
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bioRxiv - Immunology 2020Quote: ... Primed neutrophils were obtained by treating the cells for 25 mins with 10 μM cytochalasin B (Merck, #C6762) and 100 nM N-Formylmethionyl-leucyl-phenylalanine (fMLF ...
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bioRxiv - Cell Biology 2023Quote: ... pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Immunology 2021Quote: ... (final concentration of 2µg/mL) and pools of original or beta variant peptides on hydrophobic-PVDF ELISpot plates (Merck) coated with 5µg/mL anti-mouse IFNγ (AN18) ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were eluted in a final volume of 600 μL and filtered with 0.22 μm PVDF filters (Merck Millipore) before dried in a centrifugal vacuum concentrator ...
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bioRxiv - Immunology 2020Quote: ... the reduced peptide solution was purified five times using ZipTip Pipette Tips with C18 resin and 0.6 µl bed volume (Merck,) and eluted in 32.5% ACN/0.2% TFA ...
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bioRxiv - Neuroscience 2022Quote: ... was used in a concentration of 20 µM and autocamtide-2 related inhibitory peptide II (AIP-II; Merck Millipore) at 4 µM.
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bioRxiv - Plant Biology 2023Quote: ... TF complexes were eluted with 100 μl IP buffer supplemented with 200 μg/ml 3xFLAG peptide (Merck Millipore F4799). The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843 ...
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bioRxiv - Cell Biology 2023Quote: ... and were treated with various concentrations of peptides (as determined by prior Hoechst 33258 staining in serum–free media) or solvent (DMSO, 0.1% v/v, Merck) or positive control TGF-β1 10ng/ml (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... EnSC were seeded at a clonal density of 30 cells/cm2 on 1 mg/ml fibronectin-coated 6-well plates and cultured in 10% DMEM/F12 medium supplemented with basic fibroblast growth factor (bFGF; 10 ng/ml; Merck Millipore, Watford, UK) for 10 days with a 50 % media change on day 7 ...
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bioRxiv - Biochemistry 2020Quote: ... The blocking buffer was next replaced with rabbit polyclonal T22 antibody that recognises oligomeric Tau (diluted 1/2000) ((ABN454; Merck Millipore) (37) ...
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bioRxiv - Biochemistry 2020Quote: ... cells were washed 3x in PBS followed by blocking in 16% (v/v) normal goat serum (Merck Milipore, Burlington, MA, USA) supplemented with 0.1% (v/v ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then incubated overnight at 4°C in blocking solution containing one of the following primary antibodies: anti-GFAP (1:2000; Merck Millipore) and anti-Iba1 (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... cryo-sections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized and blocked with blocking solution (5% bovine serum albumin (BSA; Europa Bioproducts, EQBAH-0500) and 0.3% Triton X-100 (Merck Millipore, 93420) in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... pH 8.6) for 30 min at RT before incubation with blocking solution consisting of 3% bovine serum albumin (Sigma, Merck, Germany, #A9418) and 0.3% Triton X-100 in PBS 1X ...
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bioRxiv - Neuroscience 2024Quote: ... for 5 x 7 min and incubated 2h at room temperature (RT) with blocking solution containing 10% normal donkey serum (NDS, Merck Millipore), 3% Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000, guinea pig, AB5905, Merck Millipore) for 2 h at RT ...