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Citations for Merck :
301 - 350 of 912 citations for Brucella Blood Agar with Hemin and Vitamin K 15x100mm plate 26ml fill since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... The input control was incubated in TE buffer (10 mM Tris-HCl pH 8.0, 1 mM EDTA) supplemented with 40 mU/µL proteinase K (#P4850, Merck) for 2 h at 55°C and 1200 rpm ...
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bioRxiv - Neuroscience 2024Quote: ... AAVs in the cells and supernatant were purified by iodixanol gradient and were concentrated in phosphate-buffered saline (PBS) containing 0.001% Pluronic F68 using Amicon Ultra 100 K filter units (Merck). The titer of AAV was determined with a real-time PCR thermal cycler (Dice ...
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bioRxiv - Neuroscience 2023Quote: ... Patch electrodes were filled with KCl-K-gluconate-based internal solution with gramicidin (20 μg/mL, G5002, Merck KGaA), 5-CFDA ...
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bioRxiv - Systems Biology 2022Quote: Standard solutions of potassium (K+) and barium (Ba2+) were prepared from AnalaR salts (KCl and BaCl2) (Merck, Darmstadt, Germany). 18-crown-6 ether (99% pure ...
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bioRxiv - Immunology 2024Quote: ... The resulting LNP formulation was concentrated using an Amicon Ultra 50 K MWCO centrifugal filter device (Merck Millipore, UFC905096) and subsequently passed through a 0.22-μm syringe filter (Millipore ...
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bioRxiv - Immunology 2021Quote: ... red blood cells were lysed and cells were cultured in Iscove’s medium (Merck, Biochrom)+10% FBS (PAN Premium Lot ...
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bioRxiv - Immunology 2020Quote: ... Peripheral blood mononuclear cells (PBMC) were isolated by density gradient centrifugation using Biocoll (Merck). Cells of hematopoietic origin were isolated as live CD45+ cells (clone 2D1 ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood mononuclear cells (PBMCs) were isolated from buffy coats by Biocoll (Merck Biochrom) density gradient centrifugation ...
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bioRxiv - Immunology 2024Quote: Red blood cell lysis buffer (5X): 8.28 g of ammonium chloride (Merck, Ref. 1.01145.0500), 0.036 g of EDTA (Merck ...
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bioRxiv - Immunology 2024Quote: ... PBMCs were isolated from buffy coats by layering blood over Histopaque-1077 gradient (Merck) and centrifuging at 400xg for 25 minutes with acceleration and brake set to 1 ...
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bioRxiv - Cell Biology 2024Quote: ... red blood cells were lysed from the PEC using Red Cell Lysis buffer (Merck) and a cell count performed ...
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bioRxiv - Genomics 2023Quote: ... These assessments were conducted on 7-day old Foc strains cultured on petri dishes with potato dextrose agar (PDA; Merck, Darmstadt, Germany). An approximately 5 mm piece of the actively growing colony from each isolate was placed on PDA plates and incubated at 27°C ...
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bioRxiv - Microbiology 2024Quote: ... A 0.5 cm diameter sterile filter disk was drenched in 2 ml of AgNPs solution for 15 min and then placed in the edge of a Petri dish containing potato dextrose agar (PDA) medium (Merck, Darmstadt, Germany). A control dish comprising a disc drenched for 15 min in AgNO3 solution (1 mM ...
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bioRxiv - Microbiology 2022Quote: ... Five duplicate plates were spread individually by six gradient diluted soil suspensions from 10−2 to 10−7 dilution on 1/10 TSA medium (Tryptic Soy Agar, Merck, Darmstadt, Germany) and incubated under both aerobic and anaerobic conditions at 28 °C for two weeks ...
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bioRxiv - Microbiology 2022Quote: ... serial decimal dilutions were get set from the suspension and 100µL of each diluent was spread on MRS agar (Merck GmbH, Darmstadt, Germany). The plates were incubated at 30°C for five days in an anaerobic atmosphere ...
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bioRxiv - Immunology 2024Quote: ... the cell pellets were lysed in 0.1% TritonX-100 and serial dilutions were plated on LB agar in petri dishes (ø 85 mm; Merck, Darmstadt, Germany). Bacterial colonies were counted microscopically using a grid plate after incubation at 37°C for 24 h and 48 h ...
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bioRxiv - Biochemistry 2020Quote: Protein pellets were dissolved in 50 μl of 8 M Urea and loaded onto a 30 K centrifugal filter unit (Merck). Preparation for MS was done as described in [64] ...
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bioRxiv - Systems Biology 2020Quote: ... The reaction was incubated at 25°C for 90 minutes followed by addition of 1 μL proteinase K (Merck, 3115887001) and incubation at 37°C for 10 minutes ...
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bioRxiv - Biophysics 2021Quote: ... All the fractions containing the monomeric protein were pooled together and concentrated using amicon 10 k MWCO centrifugal filtration devices (Merck). Protein purity was determined by aRP to be 88.6% (Supplementary Figure 8) ...
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bioRxiv - Biochemistry 2022Quote: ... The peak fraction containing Na+-NQR (3.0 mL) was concentrated to 20 µL using an Amicon Ultra 100 K centrifugal filter (Merck-Millipore), and used immediately for the cryo-EM grid preparation.
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bioRxiv - Cell Biology 2022Quote: ... was repeatedly washed with PBS supplemented with protease inhibitor cocktail and reconcentrated using an Amicon® Ultra-0.5 device with 10 K MWCO (Merck) until residual urea was diluted below 10 mM ...
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bioRxiv - Immunology 2020Quote: ... MS2-FP8 VLPs were concentrated from SEC purified fractions by ultra-centrifugal filtration using Amicon® Ultra-15.0mL 100 K membrane (Merck Millipore Ltd. ...
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bioRxiv - Biophysics 2023Quote: ... Homogenization of proteins and single molecules were performed using 8 U/ml proteinase K (PK, #P4850 Sigma Aldrich now Merck) in digestion buffer (800 mM guanidine HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Clones were trial-screened for positive PCR products by making mirror plates and subjecting one of the plates to cell lysis (50mM Tris-HCl pH 8, 1mM EDTA, 0.5% Tween-20, 50-80 μg/ml Proteinase K Merck-3115801001) at 37°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... Aliquots were loaded on HP-TLC plates (HP-TLC Silica Gel 60 plates (Merck, Kenilworth, NJ)) ...
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bioRxiv - Immunology 2021Quote: ... Peripheral blood and synovial mononuclear cells were isolated by density gradient centrifugation (Biocoll, Merck Millipore) or following enzymatic digestion with hyaluronidase (10µL/mL ...
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bioRxiv - Immunology 2022Quote: Blood was collected in 3.5 ml VACUETTE tubes with clot activator (Greiner, Merck, Kenilworth, NJ) and spun down at 4000x g for 10 minutes to isolate serum ...
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bioRxiv - Biochemistry 2020Quote: ... chromatography and plates of KLT (Merck). The instruments used for structure elucidation include spectrophotometer IR (JASCO FT/IR-420 spectrophotometer) ...
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bioRxiv - Immunology 2020Quote: ... MultiScreen-HA ELISPOT plates (Merck Millipore) were coated with primary anti-IFN-γ clone CC330 (Serotec ...
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bioRxiv - Molecular Biology 2023Quote: ... TLC plates were purchased from Merck Millipore.
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bioRxiv - Cell Biology 2023Quote: ... performed in 384-well plates (Merck). The primer sequences used are listed in Table S2 ...
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bioRxiv - Immunology 2023Quote: ... Multiscreen-HA filter plates (Merck, Ireland) were coated with 5 μg/mL of M1 extract in carbonate coating buffer overnight at 4°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... Nunc MaxiSorp ELISA plates (M9410, Merck), 10% BSA ELISA reagent diluent/blocking solution concentrate (DY995 ...
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bioRxiv - Immunology 2024Quote: ... PVDF type MSIP plates (Merck, Germany) were pre-treated with 15µl 35% ethanol for <1min and coated with IgA mAB MT45a overnight in the fridge ...
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bioRxiv - Neuroscience 2024Quote: 24-well transwell plates (Merck, CLS3470) were coated with Matrigel (Corning ...
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bioRxiv - Cell Biology 2023Quote: ... The mixture was purified by scraping from a TLC plate (Silica gel 60 F254 TLC plates, Merck) and eluting (25% ethyl acetate/hexane ...
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bioRxiv - Biophysics 2021Quote: ... pH 7.5 and 150 mM NaCl) and concentration processes performed using a dialysis Amicon Ultra 10 K (Merck Millipore, Darmstadt, Germany).
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bioRxiv - Systems Biology 2021Quote: ... Excess FSBA reagent was removed by ultracentrifugation with 15 mL 10 K MWCO Amicon® Ultra-4 centrifugal filter units (Merck) at 3,500 × g at RT ...
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bioRxiv - Biophysics 2022Quote: ... followed by incubation in LI-COR blocking buffer containing rabbit polyclonal anti-P2X2 (#APR-003, Alomone labs; 1:2000) and mouse anti-Na+/K+-ATPase (05-369; EMD Merck Millipore) at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... After incubation the mixtures were buffer exchanged in centrifugal filter units (Amicon Ultra, 0.5 ml, 10 K, Merck Millipore, Darmstadt, Germany) washed three times with 50 µl of sodium phosphate buffer (20 mM Na2HPO4 ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatants were then used to quantify the level of adiponectin using Mouse Adiponectin ELISA kit (Merck Millipore #EZMADP-6 K) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... the supernatant was concentrated by centrifugation at 1000 g at 4 °C through Amicon® Ultra 15 mL Centrifugal Filters (100 K; Merck). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... Peak fractions containing the Swi2-Swi5 complex were concentrated using an Amicon Ultra-4 (50 k) centrifugal filter (UFC805008, Merck Millipore). After cleavage ...
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bioRxiv - Biophysics 2024Quote: ... nsp16 and nsp7-11 were exchanged by six rounds of dilution and concentration in centrifugal filter units (Amicon Ultra, 10 K MWCO, Merck Millipore).
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bioRxiv - Cancer Biology 2023Quote: ... the unencapsulated FITC-dextran or FITC-SAP was removed by washing and filtration using Amicon Ultra centrifugal filters (100 K device, Merck Millipore). The incorporation of FITC-dextran and FITC-SAP into the nsEVs was confirmed by spectrofluometer analysis (FP-6200 ...
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bioRxiv - Microbiology 2022Quote: ... Sodium dodecyl sulfate (final concentration, 1 mg/mL) and proteinase K (final concentration, 1 mg/mL; MERCK Group Japan, Tokyo, Japan) were added to the sample ...
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bioRxiv - Neuroscience 2024Quote: ... The AAV titer was quantified usizg PCR (5′-TGA GTC ACC CAC ACA AAG GA-3′ and 5′-CCA AGC TGG CCT AAC TTC AG-3′) after proteinase K treatment (Merck Millipore). Under anesthesia with a mixture of medetomidine (0.3 mg/kg ...
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bioRxiv - Biophysics 2024Quote: Elution fractions containing the MS-ring composed of FliFG fusion proteins with the FliG-G214S or FliG-G215A mutations were concentrated 5-fold using an Amicon Ultra 100 K device (Merck Millipore). The G214S and G215A mutants were diluted 50- and 100-fold ...
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bioRxiv - Biophysics 2024Quote: ... Fractions containing FF A39G were concentrated and exchanged in the NMR buffer (100 mM sodium acetate, 200 mM sodium chloride, pH = 5.7) with Amicon Ultra 15 MWCO = 3 K (Merck, Darmstadt, Germany). The cleaved protein product was 71-residues long with no cloning artifacts at either end (GSQPAKKTYT WNTKEEAKQA FKELLKEKRV PSNASWEQGM KMIINDPRYS ALAKLSEKKQ AFNAYKVQTE K).
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bioRxiv - Cell Biology 2024Quote: ... The cell culture medium was concentrated using Amicon® Ultra-15 3 K centrifugal filters (Merck Millipore Ltd., Burlington, MA, USA) into a final volume of 80 ml ...