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Citations for Merck :
3251 - 3300 of 5053 citations for 8 chloro 10 3 dimethylamino propyl phenothiazin 1 ol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... mouse anti-EF1α (1: 40 000, Merck Millipore), anti-BrdU clone B44 (1 ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked in 5% skim milk in TBS for 1 hour and probed with mouse γ/9d 2G10.2 (1:1000, MERCK MABT1335), rabbit anti mouse IgG (1:3000 ...
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bioRxiv - Cell Biology 2022Quote: ... DLD-1-WT mCherry-Sec61β and DLD-1-C-H3 mCherry-Sec61β stable cell lines were generated by GeneJuice (Merck Millipore) transfection of mCherry-Sec61β into the respective parental lines ...
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bioRxiv - Neuroscience 2019Quote: ... blocked using 1% BSA in TBST and incubated with primary anti-TH antibody (dilution 1:500; AB152, Merck Millipore, Schaffhausen, Switzerland). 24h later ...
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bioRxiv - Microbiology 2019Quote: ... the cell pellet was resuspended in 50% LR White resin (1:1 volume of LR White resin (London Resin Company) and 95% ethanol (Merck Millipore)) and the cell suspension was stored in 4°C till further use ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were detected by incubation with primary antibodies (SOX2, Bio-techne, AF2018, 1: 1000 and α-Tubulin, Merck, T6199, 1:2000) and secondary antibodies (Donkey anti-Goat IRDye® 800CW ...
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bioRxiv - Neuroscience 2020Quote: ... the following primary antibodies or lectin were used: an anti-aggrecan rabbit antibody (1:4500 for DAB staining; 1:500 for fluorescence staining; AB1031, Merck Millipore), an anti-GABA chicken antibody (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... rabbit polyclonal, Synaptic System, 163 002, 1:600; VGLUT1: guinea pig polyclonal, Merck Millipore, AB5905, 1:5000; MAP2: rabbit polyclonal, Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... MAP2 and/or PSD-95 were immunostained using primary antibodies diluted in PBS-BSA 1% for 30 min (SP: rabbit polyclonal, Synaptic System, 163 002, 1:600; VGLUT1: guinea pig polyclonal, Merck Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were transferred to well-plates for exposure to two primary antibodies (RFP antibody 5F8 rat for mCherry, RRID: AB_2336064, Chromotek, 1:1000; and mouse-monoclonal anti-CaMKIIα, RRID: AB_309787, Merck Millipore, 1:300) which were added to 0.1 M PBS-Tx 0.2% containing 5% normal goat serum ...
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bioRxiv - Cell Biology 2022Quote: ... blots were blocked for 1 h and incubated overnight at 4 °C with anti-Amyloid fibrils (OC, 1:5000, Merck-Millipore), anti-prefibrillar oligomers (A11 ...
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bioRxiv - Cell Biology 2022Quote: ... Lysates were centrifuged for 5 min at 20 000 x g and supernatants were immunoprecipitated for 1 h rotating at 4°C with mouse anti-GFP antibody (1 µg antibody per sample, 11814460001 Merck/Sigma) and 20 µl DynabeadsTM Protein G (10004D ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... neurons were stained with 1 μg/ml HOECHST-33342 (H-33342) and 1 μM calcein-AM (both from Merck, Darmstadt, Germany) one hour prior to the imaging ...
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bioRxiv - Synthetic Biology 2020Quote: ... Growth experiments were performed on YNB medium with 1 or 2 % methanol supplemented with 1 g/L yeast extract (Merck 103753). Optical density readings at 600 nm (OD600 ...
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bioRxiv - Immunology 2021Quote: Cytokine and chemokine levels produced by PCLS after 2 dpi were assessed in a Multiplex assay in supernatants (dilution 1:2) with MILLIPLEX® Bovine Cytokine/Chemokine Panel 1 (BCYT1-33K-PX15, Merck) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Sodium dodecyl sulfate (final concentration, 1 mg/mL) and proteinase K (final concentration, 1 mg/mL; MERCK Group Japan, Tokyo, Japan) were added to the sample ...
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bioRxiv - Bioengineering 2022Quote: ... freeze-dried mucin-MA and unreacted mucin samples were separately dissolved at 1 mg mL−1 in 0.1 M sodium bicarbonate buffer pH 8.5 (Merck, Kenilworth, NJ, USA). Then ...
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bioRxiv - Cell Biology 2024Quote: ... Primary antibodies were incubated for 1 hour using the following antibodies: 1:500 rabbit anti-H3S10ph (Sigma-Aldrich, Merck, 06-570), 1:500 mouse anticyclin B1 (BD Pharmingen ...
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bioRxiv - Neuroscience 2024Quote: ... The different tau isoforms 3R and 4R were checked with monoclonal RD3 (1:200 dilution) (clone 8E6/C11) and RD4 (1:200 dilution) (clone 1E1/A6) antibodies (Merck Millipore). Other antibodies used were rabbit anti P62/ SQSTM1 (1:100 dilution ...
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bioRxiv - Biochemistry 2023Quote: ... and lysed in recombinant tau-lysis buffer (1 mM benzamidine, 1 mM PMSF, 1x cOmplete™, EDTA-free Protease Inhibitor Cocktail mix (Merck), 14 mM b-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... R&D, AF2408), Goat-Anti Human SOX17 (1:100, R&D, AF1924) or Mouse Anti-Human Beta-Tubulin (1:1000, Merck, T8660) primary Chicken Anti-Human MAP2 (1:2000 ...
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bioRxiv - Biophysics 2023Quote: ... 5 µL of 25 µM GSK3β-phosphorylated tau fibrils were sonicated for 1 minute using a water bath sonicator (Bandelin Sonorex digitec) and then mixed with 1 µL of 1 mg/mL pronase (53702, Merck-Millipore). The mixture was then quickly added to the Quantifoil 2/1 grids and plunged-frozen after an incubation of 5 seconds using a Leica EM GP2 automatic plunge freezer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were incubated with primary antibodies diluted in blocking solution (anti-MAP2, 1:500, chicken, ab5392, Abcam; and anti-vGlut1 1:1000, guinea pig, AB5905, Merck Millipore) for 2 h at RT ...
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bioRxiv - Microbiology 2020Quote: ... Glycolipid extracts of up to 15 μl were applied evenly onto half TLC silica plates (20 x 10 cm, Merck KGaA, Darmstadt, Germany). After drying ...
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bioRxiv - Microbiology 2019Quote: ... Chlorophyll a content was determined using a microplate reader (BMG Polarstar Optima) after filtration of the sub-samples (5 to 10 mL) through Whatman GF/F glass circles (25 mm) (Merck KGaA, Darmstadt, Germany) and 90% acetone extraction of the filtrate for 24 h in the dark at 4 °C [74].
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bioRxiv - Biophysics 2019Quote: ... ES products from all three days were thawed at 4 °C and pooled to be concentrated 720 times with Amicon® Ultra-15 Centrifugal Filter Unit 10 kDa cut-off (Merck, cat#UFC901024). The concentrate was used for EV separation.
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bioRxiv - Bioengineering 2019Quote: ... was diluted with ultrapure water (30 mL LUDOX® TM50 to 400 mL water) followed by addition of ammonia (10 mL) (NH3, 28-30%, Merck, Darmstadt, Germany) and (3-aminopropyl)trimethoxysilane (24 mL ...
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bioRxiv - Cell Biology 2021Quote: ... 1.5E05 cells were seeded onto a T25 Matrigel coated flask in StemFlex medium supplemented with 10 µM rock inhibitor (Merck Millipore, Y-27632). Medium was refreshed the following day without rock inhibitor and lived cells were sent over to Cell Line Genetics when 20-30% confluence was reached ...
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bioRxiv - Cell Biology 2021Quote: ... The fractions containing the target proteins were pooled and concentrated to less than 0.5 ml using an Amicon Ultra concentrator (Merck Millipore; 10 kDa MWCO). The concentrate was applied to a Superdex 200 Increase 10/300 GL size exclusion chromatography column (GE Healthcare ...
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bioRxiv - Microbiology 2022Quote: ... Suspensions were passed through a 40-µm strainer into a 50 ml tube and rinsed again in 12 ml of DMEM with 10% Fetal bovine serum (FBS) (Merck, Kenilworth New Jersey) in 15 ml tubes ...
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bioRxiv - Plant Biology 2022Quote: ... followed by a 20 min centrifugation at 4°C and 4000 rpm using 10-kD Amicon®Ultra centrifuge filter (Merck, Darmstadt, Germany) to remove free (proteinun bound ...
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bioRxiv - Molecular Biology 2019Quote: ... was thawed at room temperature and vortexed extensively before being concentrated to 800 µl using a 10 kDa centrifugal filter device (Centricon Plus-70, Merck Millipore, Massachusetts, USA). The concentrated urine sample was resuspended in 3.2 ml of a 50% iodixanol solution and layered on the bottom of a 17 ml Thinwall Polypropylene Tube (Beckman Coulter ...
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bioRxiv - Physiology 2019Quote: ... in 35 mm Petri dishes were incubated for 10 min in steady state E2 medium (controls) or E2 + 50 or 250 mM NaCl (Merck, #106404, Darmstadt, Germany) at 28°C under white light illumination ...
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bioRxiv - Biochemistry 2019Quote: ... Eluted protein was concentrated using Amicon Ultra-15 centrifugal filter unit with nominal molecular weight limit of 10 kDa (Merck Millipore, Burlington, MA), loaded on a HiLoad 26/600 Superdex 200 column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The resulting cell free supernatant (CFS) was concentrated through a 10 kDa polyethersulfone membrane disc using an Amicon® Stirred Cell (Merck Millipore, Germany) to a final volume of 50 mL ...
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bioRxiv - Cell Biology 2021Quote: ... BHK-2P maintained either in Glasgow’s Minimal Essential Medium (GMEM) with 10% FBS (“line #2”) or adapted to the ACFM Cellvento™ BHK200 (Merck KGaA, Darmstadt, Germany) in two different processes (lines “#4” and “#5”) ...
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bioRxiv - Developmental Biology 2022Quote: ... The dehydrated samples were then incubated in a 1:1 mixture of propylene oxide and absolute ethanol for five minutes at RT followed by a two times 10 minutes incubation in pure propylene oxide (Sigma-Aldrich, Merck, Darmstadt, Germany), and a 30 minutes incubation in a 1:1 mixture of propylene oxide and Spurr resin (Low Viscosity Spurr Kit ...
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bioRxiv - Cancer Biology 2022Quote: ... The remaining peptides were loaded onto primed custom-made HILIC SPE micro-columns packed with ZIC-HILIC resin (10 μm particle size, 200 Å pore size, kindly provided by Sequant/Merck, Umea, Sweden) onto supporting C8 disks (Empore ...
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bioRxiv - Immunology 2024Quote: ... The cells were washed three times with 200 µl/well of PBS/Tween and incubated for 10 min at room temperature with 50 µl/well of 3,3‘,5‘,5‘-tetramethylbenzidine (TMB)/H2O2 peroxidase substrate (Merck KGaA, cat. no. T4444). The reaction was stopped by addition of 50 µl/well of 1 M HCl and absorbance read at 450 nm using a GloMax microplate reader (Promega ...
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bioRxiv - Immunology 2024Quote: ... The cells were incubated for 24h at 37°C and 5% CO2 in the presence of 10-9 M of mifepristone (Merck KGaA, Darmstadt, Germany) to allow expression of the G protein ...
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bioRxiv - Synthetic Biology 2023Quote: ... by centrifuging the purified enzyme preparations (4,000×g, 4°C) using AmiconTM Ultra-15 centrifugal filters of 10 kDa-pore size (Merck-MilliPoreSigma, Burlington, MA, USA). The absorbance at 280 nm (A280 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The protein preparation was then concentrated to 300 – 500 μL volume using Amicon Ultra centrifugal filter units with 10 kD cut-off (Merck KGaA, Darmstadt, Germany) and injected into Superdex 75 10/300 GL size exclusion column attached to ÄKTA micro chromatography system (Cytiva ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Samples were dissolved in 50 μL of Milli-Q water and filtered (0.20 μm pore size, 10 mm diameter, Merck Millipore, Burlington, MA, US)71.
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bioRxiv - Biochemistry 2023Quote: ... Proteins purified under native conditions were concentrated to 400 μL using a 10 kDa molecular weight cutoff (MWCO) Amicon Ultra-4 centrifugal spin concentrator (Merck-Millipore, Burlington, MA) and purified by size-exclusion chromatography using a Superdex 200 Increase 10/300 column (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... The resulting Fabs were buffer exchanged into PBS and then concentrated down using Amicon® 10 kDa Ultra-15 Centrifugal Filter Units (Merck Millipore UFC9010). Selected fractions resulting from the size-exclusion chromatography were pooled together and concentrated again for later use.
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bioRxiv - Neuroscience 2023Quote: ... dermis and epidermis were mechanically separated and the dermal part was collected in fibroblast cultivation medium (DMEM/F12 + 100 U/ml penicillin 100 µg/ml streptomycin [pen/strep; both: Thermo Fisher Scientific, Waltham, MA, USA] + 10% fetal calf serum [FCS; Merck, Darmstadt, Germany]).
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bioRxiv - Genetics 2023Quote: ... and neo-resistance MEFs were established from WT Crl:CD1 (ICR) or ICRneo [27] foetuses at 14 d post-coitus and inactivated using 10 μg/mL mitomycin C (#M0503; Merck KGaA, Darmstadt, Germany). MEFs were cultured in the primary medium [Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Neuroscience 2023Quote: ... TSPO-/- MPAs were grown in T25 flasks until confluent before being stored in liquid nitrogen (10% v/v dimethyl sulfoxide (DMSO; catalogue # D2650, Merck Life Sciences (UK)) until TSPO genotypes had been confirmed ...
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bioRxiv - Biochemistry 2023Quote: Purified NanoLuc was concentrated to a final concentration of ∼10 mg/mL using Centrifugal Filter Units AmiconR Ultra-15 UltracelR-3K (Merck Millipore Ltd., Ireland). Concentrated NanoLuc was mixed with a 4 molar excess of FMZ non-oxidizable analogue azaFMZ (stock solution of azaFMZ was 10 mM in isopropanol) ...
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bioRxiv - Cell Biology 2024Quote: ... Peak fractions were pooled and concentrated using an Amicon Ultra-0.5 centrifugal filter device with a molecular weight cut-off of 30 or 10 kDa (Merck, cat. # UFC503024 or UFC501008). D-desthiobiotin in the peak fraction was diluted more than 100-fold by a repeating dilution with EB and the centrifugal concentration ...