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Citations for Merck :
3251 - 3300 of 5854 citations for 6 propan 2 yl 1 3 5 triazine 2 4 diamine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2021Quote: ... with 0.2 % m/v Triton-X100 and 5% Normal Goat Serum (m/v) (S26-LITER, Merck Life Science UK LTD) (PBST.NGS ...
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bioRxiv - Cell Biology 2021Quote: ... HL-60 and MS-5 cells cultured alone and in co-culture were incubated for 24 hours in 50 μM H2O2 (Merck) complete cell culture medium ...
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bioRxiv - Neuroscience 2020Quote: ... two custom-made Teflon containers of 5 mm diameter were filled with 10 μl of odor substance (n-amylacetate, AM; CAS: 628-63-7, Merck, Darmstadt ...
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bioRxiv - Microbiology 2021Quote: ... Identification of metabolites for LC-MS was performed with a ZIC pHILIC column (150 mm × 4.6 mm, 5 μm column, Merck Sequant) coupled to high-resolution Thermo Orbitrap QExactive (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... chromatographic separation was performed on ZIC-pHILIC column equipped with a guard (5 µm, 4.6 × 150 mm, SeQuant®, Merck). The mobile phase (A ...
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bioRxiv - Biophysics 2020Quote: ... 50 μL of sample was injected onto a Discovery BIO Wide Pore C18 column (15 cm x 4.6 mm, 5 μm column with a guard column) (Supelco, Merck, UK) and eluted on a gradient of 95% water + 0.1% acetic acid and 5% acetonitrile + 0.1% acetic acid to 5% water + 0.1% acetic acid and 95% acetonitrile + 0.1% acetic acid at a 0.8 mL/min flow-rate over 40 mins ...
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miR-206 inhibits estrogen-induced proliferation and invasion of ER-α36 positive gastric cancer cellsbioRxiv - Cancer Biology 2021Quote: ... 20 μL of the MTT solution was added to each well (5 mg/ml, Sigma-Aldrich; Merck KGaA, Darmstadt, Germany). Then ...
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bioRxiv - Cell Biology 2021Quote: ... against a final buffer (20mM Hepes, 200 mM NaCl, 5% Glicerol, 2mM DTT) and concentrated with appropriate MW cut-off Vivaspin columns (Merck). The concentration of purified proteins was determined by colorimetric assay (Bio-Rad DC Protein Assay ...
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bioRxiv - Cell Biology 2021Quote: The samples were loaded onto a 5–20% gradient SDS-PAGE gel (Wako, Osaka, Japan) and transferred to an Immobilon-P Transfer Membrane (Merck). Antibodies were diluted with Signal Enhancer HIKARI for Western Blotting and ELISA (Nacalai Tesque) ...
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bioRxiv - Microbiology 2022Quote: ... Separation in the HPLC was carried out using a SeQuant ZIC-pHILIC column (PEEK 150 × 2,1 mm, 5 μm, 110 Å, Merck) at 30 °C with an CH3CN (buffer A ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated for 72h with the indicated concentrations of the respective drugs and further incubated for 3h with 5 mg/ml MTT (Merck). Finally ...
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bioRxiv - Pathology 2019Quote: ... For MALDI-TOF/TOF/MS analysis dried peptides were dissolved in peptide resuspension solution (0.1% TFA in 5% ACN) and desalted/concentrated using C18 zip tips (Merck Millipore) as per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Chromatographic separation was achieved using a silica-based SeQuant ZIC-pHILIC column (2.1 mm × 150 mm, 5 µm, Merck, Germany) with elution buffers consisting of (A ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Membranes were blocked using 5% non-fat dry milk in Tris-buffered saline with 0.1% Tween 20 (655204, Merck Millipore) and probed with the respective primary and secondary antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... pregnant mice were injected intraperitoneally with 375 μg doxycycline (75 μl of a filter sterilized, 5 mg/ml solution of Doxycycline Hyclate (Merck) dissolved in PBS) ...
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bioRxiv - Microbiology 2021Quote: ... The separation in the HPLC was carried out using a SeQuant ZIC-pHILIC column (PEEK 150 × 2.1 mm, 5 μm, 110 Å, Merck) at 30°C with an CH3CN (buffer A ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cellular extract was spun down and the supernatant has been incubated with His-binding resin (Merck 69670-5) for 10-30 min at room temperature ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... supernatants containing RBD or soluble Spike were concentrated and diafiltrated against 20 mM sodium phosphate buffer containing 500 mM NaCl and 20 mM imidazole (pH 7.4) using a Labscale TFF system equipped with a 5 kDa cut-off Pellicon XL device (Merck Millipore). The His-tagged proteins were captured using a 5 mL HisTrap FF crude column connected to an ÄKTA pure chromatography system (both from Cytiva ...
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bioRxiv - Molecular Biology 2021Quote: ... and the plates were incubated at 33 °C in a 5% CO2 atmosphere in fresh medium with 0.25% Hoechst (Merck-Millipore) to stain the DNA ...
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bioRxiv - Microbiology 2019Quote: ... samples were fixed in 2.5% (v/v) glutaraldehyde in HBSS on IsoporeTM membrane TMTP filters with pore size 5 µm (Merck-Millipore) for 30 min followed by washing thrice with HBSS buffer (10 min each) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 mM MgCl2 buffer at a final concentration of 5 mg/mL using three consecutive cycles of freezing in liquid nitrogen and thawing in an ultrasonic bath (Merck). The rehydrated lipid solutions were extruded 21 times through a 100 nm diameter pore size polycarbonate membrane (Avanti Polar Lipids Inc.) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting cell pellet was freeze-thawed thrice and re-suspended to 10% (w/v) in a lysis buffer containing 5% (v/v) Polysorbate 80 and 20 U/mL benzonase (Merck). After 1h incubation at 37°C ...
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bioRxiv - Physiology 2022Quote: ... They were then permeabilized with 0.02% Triton X-100 in PBS for 15 min and blocked with 5% bovine serum albumin (Sigma-Aldrich, Merck) in PBS for 3 h ...
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bioRxiv - Microbiology 2022Quote: ... fixed with cold methanol for 5 min and permeabilized with PBS containing 0.1% Triton X-100 (Merck Life Science, T8787). For the staining of acidic organelles ...
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bioRxiv - Microbiology 2022Quote: ... cells were cultured in 25 cm2 flasks at 37°C and 5% CO2 using advanced Dulbeccos modified Eagle medium (DMEM; TFS) with the addition of 10% fetal bovine serum (Merck), 50 U/mL penicillin and 50 μg/mL streptomycin (TFS) ...
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bioRxiv - Neuroscience 2022Quote: ... Cubes of 1 cm3 were cut and incubated overnight in an aqueous solution of 150 mM NaCl (S9888, CAS 7647-14-5, Merck) and 10 µM fluorescein (46955 ...
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bioRxiv - Biochemistry 2023Quote: ... Briefly this involved separation of metabolites using a ZIC pHILIC column (150 mm × 4.6 mm, 5 μm column, Merck Sequant) before entering an Orbitrap Q-Exactive (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... 1 × 105 cells were plated per well and were further cultured in the presence or absence of 5 µM oligomycin A (Merck) at 37 °C in a humidified chamber containing 5% CO2 and 1% O2 for 2 hours ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were subsequently used for hydrophilic interaction liquid chromatography-mass spectrometry analysis (Dionex UltiMate 3,000 RSLC, Thermo Fisher, using a 150 × 4.6 mm, 5 µm ZIC-pHILIC column, Merck Sequant). The column was maintained at 40°C and samples were eluted with a linear gradient (20 mM ammonium carbonate in water and acetonitrile ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was then quenched by washing the cells three times with ice-cold PBS buffer containing 5 mM Trolox (Sigma-Merck), 10 mM sodium ascorbate ...
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bioRxiv - Biophysics 2024Quote: ... and the elution fraction was concentrated to 5 ml using an Amicon Ultra Centrifugal Filter Unit (30 kDa MWCO, Merck). The concentrated protein was applied to size exclusion chromatography using a HiLoad 16/600 Superdex 200 pg (Cytiva ...
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bioRxiv - Biophysics 2024Quote: Glass coverslips (Paul Marienfeld GmbH, 24 x 50 mm, 170 ± 5 μm) were overnight incubated in 100 mM Sulphuric acid (Merck). Afterwards the coverslips were rinsed consecutively with Milli-Q water ...
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Neuron-astrocyte metabolic coupling facilitates spinal plasticity and maintenance of persistent painbioRxiv - Neuroscience 2022Quote: ... the sections were equilibrated to room temperature and then washed twice for 5 minutes (min) in PBST (0.1% Triton X-100 (Merck, #108603)/PBS) ...
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bioRxiv - Pathology 2023Quote: ... Then sections were blocked for 45 min using a blocking buffer containing 5% goat or donkey serum (depending on the host species of the secondary antibody) (#G9023 and #D9663, Merck) and 0.3M glycine (#56-40-6 ...
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bioRxiv - Immunology 2023Quote: ... Titers were determined by transduction of Jurkat cells (ACC 282; DSMZ) in presence of 5 µg/ml Polybrene Infection/Transfection Reagent (Merck). After 48 h ...
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bioRxiv - Developmental Biology 2023Quote: ... All the oocytes harvested from COCs were transferred to activation medium composed of KSOM medium supplemented with 5 mM strontium chloride (13909, Merck) and 2 mM EGTA (A0878 ...
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bioRxiv - Pathology 2023Quote: ... lobus accessorius) were collected and fixed in 10 % neutral-buffered formalin (Chemie Vetrieb GmbH & Co) or 5 % glutaraldehyde (Merck KGaA) for microscopic and ultrastructural evaluation respectively.
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bioRxiv - Plant Biology 2023Quote: ... Sheared chromatin was incubated with 10 µg of α-H4.V or 5 µg of α-H4K5Ac (Merck 07-327) or 4 µg of α-H4 (Abcam ab10158 ...
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bioRxiv - Microbiology 2023Quote: ... A SeQuant® ZIC®-HILIC 5 μm particle size analytical column 150 x 4.6 mm (Merck KGaA, Darmstadt, Germany) and a Acquity UPLC BEH C18 column 1.7 μm particle sized analytical column 2.1 × 100 mm connected to an Acquity UPLC BEH C18 VanGuard Pre-column ...
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bioRxiv - Biochemistry 2023Quote: ... HeLa cells were cultivated at 37°C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peak fractions were pooled and concentrated to 5 μM (0.7 mg/mL) final concentration using an Amicon Ultra-15 (Merck Millipore) for further analysis.
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bioRxiv - Microbiology 2023Quote: ... column 5 µm 150 x 4.6 mm with a 20 x 2.1 mm ZIC-pHILIC guard column (both Merck Millipore, Australia) (25 °C) ...
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bioRxiv - Microbiology 2023Quote: Samples separation with chromatography was performed on the SeQuant ZIC-HILIC column (5 μm, 200 Å, 150x2.1 mm, Merck, Germany) with a flow rate of 0.6 mL/min ...
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bioRxiv - Molecular Biology 2023Quote: ... samples were centrifuged at 14,000 rpm at room temperature for 5 minutes or filtered in multiscreenHTS-HV 0.45 µm 96 well filter plates with PVDF membranes (Merck Millipore) to remove cell debris and nucleic acid aggregates ...
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bioRxiv - Biophysics 2024Quote: ... HeLa cells were cultivated at 37 °C under 5% CO2 in MEM with Earle’s salts supplemented with 10% FBS superior (Merck KGaA), 2 mM L-Alanyl-L-Glutamine (Biochrom) ...
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bioRxiv - Biochemistry 2024Quote: ... for 5-10 min at room temperature to reveal bound cytokine and reaction was blocked by adding HCl 2N (Merck) solution (20 μL/well) ...
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Gliflozins, sucrose and flavonoids are allosteric activators of lecithin:cholesterol acyltransferasebioRxiv - Biochemistry 2024Quote: ... PAM (0.1 mg/mL in DMSO) or DMSO were incubated with LCAT (5 μg/mL in assay buffer + 60 μM albumin (Merck)) 1:100 v/v at 37°C gently shaken ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatin immunoprecipitation was performed using 5-10 μg of primary mouse monoclonal ANTI-FLAG® M2 antibody (Merck, F1804-200UG) in 250 μL LB3 with 1% Triton X-100 overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was centrifuged (3215 g for 5 min) and then filtered (Merck Millex™ Syringe Filter, PES, 0.22 μm). In the enrichment phase ...
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bioRxiv - Molecular Biology 2024Quote: ... Milli-Q water suitable for LC-MS analysis was freshly obtained from Millipore Milli-Q® Advantage A10 system (18,2 MΩ×cm, <5 ppb) equipped with a LC-Pak® Polisher filter unit (#LCPAK0001) purchased from Merck Millipore (Darmstadt ...