Labshake search
Citations for Merck :
3151 - 3200 of 4915 citations for Human Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2024Quote: ... Sephacryl S-200 and Sephadex G-25 (PD-10 columns) were from Amer-sham-Pharmacia (Cytiva, now purchasable from Merck Life Sciences, Madrid, Spain). DEAE-cellulose DE52 was from Whatman (now purchasable from Merck Life Sciences ...
-
bioRxiv - Immunology 2024Quote: ... the differentiating cells were transduced with mVDR and Gfi1-expressing retrovirus particles in the presence of 10 µg/mL of Polybrene transfection reagent (Merck Millipore, Burlington, MA, USA). These cells were then subsequently expanded on day 3 and finally harvested for flow cytometry on day 5 ...
-
bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies (as appropriate) were added for 1 hour at room temperature (goat anti-rabbit/ goat anti-mouse, IgG, HRP conjugated; Merck Millipore (1:5000)) before visualisation using enhanced chemiluminescence (1.25 mM luminol ...
-
bioRxiv - Cancer Biology 2022Quote: ... filters were incubated for 1 hour at RT with either goat-anti rabbit (1:5000) or goat-anti mouse (1:2000) horseradish peroxidase (HRP)-conjugated secondary antibodies (Merck Millipore, Darmstadt, Germany). Blots were developed using SuperSignal West Pico Chemiluminescent Substrate or Femto Maximum Sensitivity (Thermo Fisher Scientific) ...
-
bioRxiv - Neuroscience 2022Quote: ... Slices were subsequently washed with PBS and incubated for 1 h with the proper secondary antibodies (1:400, goat anti-rat Alexa-Fluor® 568, ab175476, abcam; 1:400, goat anti-mouse Alexa-Fluor® 488, AP124JA4, Merck Millipore). For nuclear counterstaining slices were incubated with DAPI (1:1000 ...
-
bioRxiv - Cell Biology 2020Quote: ... All lysis buffers were supplemented with inhibitors of proteases (1/100 (v/v)) and phosphatases (1/50 (v/v)) (P8340 and P5726, Merck/Sigma-Aldrich, UK). Protein concentration was quantified using a BCA protein assay (23252 ...
-
bioRxiv - Cell Biology 2019Quote: ... anti-rabbit IgG (goat, coupled to Alexa 488, Thermo Fisher, A32731, 1:500 and donkey, coupled to Cy3, Merck Millipore, AP182C, 1:1000), anti-goat IgG (donkey ...
-
bioRxiv - Neuroscience 2021Quote: ... unbound primary antibodies were removed by washing in TBST and the blots were incubated with HRP-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG and IgM HRP conjugated antibodies (1:4000, Merck EMD Millipore, Ap130P), or goat anti-rabbit IgG antibody ...
-
bioRxiv - Biochemistry 2022Quote: ... Blocked membranes were incubated (1 hour, 1/5000 dilution, RT) with mouse anti-phosphotyrosine (clone 4G10) directly conjugated to HRP (4G10-HRP; Merck/ Millipore, RRID: AB_310779), or unlabelled 4G10® Platinum (Merck/ Millipore ...
-
bioRxiv - Physiology 2023Quote: ... they were incubated in their respective secondary antibodies for 1 h at room temperature (goat anti-rabbit, IgG, HRP conjugated; Merck Millipore (1: 5000) in 5% milk-TBST ...
-
bioRxiv - Neuroscience 2024Quote: ... and incubated o/n at 4°C with the primary antibodies (Rabbit-antiGFAP: MAB3402, Merck Millipore, 1:500; Mouse-antiNeuN: MAB377, Merck Millipore, 1:500) diluted in the blocking solution ...
-
bioRxiv - Microbiology 2024Quote: ... 0.5 mL of a UPLC-grade water:methanol (3:1, v/v) solution with 1:500 diluted 13C-and 15N-labeled amino acids standard mix (Sigma-Aldrich, Merck, Darmstadt, Germany) was added to the tubes ...
-
bioRxiv - Microbiology 2023Quote: ... the cells were stained with goat anti-rabbit Alexa Fluor® 555 (Thermo, 1:1,000) and mouse anti-IE Alexa Fluor® 488 (Merck, 1: 1,000) for 1 hour at RT ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: When the tumor sizes reached to 80 mm3 animals were treated with a PFKFB3 inhibitor 3-(3-pyridinyl)-1-(4-pyridinyl)-2-propen-1-one) (3PO) (Sigma-Aldrich, Merck, Overijse, Belgium). The animals received intraperitoneal (i.p. ...
-
bioRxiv - Biochemistry 2021Quote: ... Apoptotic cells were stained using ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Millipore, MA, USA) according to manufacturers instructions.
-
bioRxiv - Molecular Biology 2022Quote: ... RNA was extracted using a Spectrum Plant Total RNA kit (Sigma-Aldrich, Merck KGaA, Darmstadt, Germany).
-
bioRxiv - Physiology 2022Quote: ... To detect total protein carbonylation by immunoblot we used the OxyBlot Protein Oxidation Detection kit (Merck) following the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... Cell lysis and membrane protein extraction was performed using the ProteoExtract Native Membrane kit (Merck Millipore) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... Secondary antibody incubation was followed by 0.1% TBST wash and developed using an ECL kit (Merck). Densitometry analysis was used for quantitation of protein expression levels using Image J ...
-
bioRxiv - Microbiology 2020Quote: ... Cell death was detected by Apoptag® Red in situ apoptosis detection kit (Merck, catalog # S7165) which labels apoptotic cells ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were measured for protein concentration using a Non-Interfering protein Assay kit (cat#488250, Merck Millipore ...
-
bioRxiv - Neuroscience 2022Quote: ... Immunoprecipitation of m6A-modified transcripts was carried out with Magna MeRIP™ m6A kit (Merck-Millipore) following the manual ...
-
bioRxiv - Cancer Biology 2022Quote: Cell migration was assessed using the QCM Chemotaxis Cell Migration Colorimetric Assay kit (ECM506, Merck, USA). Briefly ...
-
bioRxiv - Bioengineering 2022Quote: ... The live/dead cells were stained with the Calcein-AM/PI Kit (Merck, 04511-1KT-F) according to the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2022Quote: ... and LX-2 cells were labeled with a PKH67 Green Fluorescent Cell Linker Kit (Merck, PKH67GL) according to the manufacturer’s protocols ...
-
bioRxiv - Cell Biology 2024Quote: ... The full-length MDM2 coding sequence was isolated from Addgene plasmid #16233 (pcDNA3 FRT MDM2) by PCR using the KOD hot start polymerase kit (Merck #71086), using primers that included a 5’ BamHI restriction site ...
-
bioRxiv - Cell Biology 2024Quote: ... HRP-mediated substrate development was performed using Amersham detection system (ECL Western Blot detection kit, Merck). Blots were imaged using ChemiDoc™ MP Imaging System (Bio-Rad).
-
bioRxiv - Microbiology 2023Quote: Apoptosis was detected using the ApopTag Peroxidase In Situ Apoptosis Detection Kit (Merck Catalogue No. S7100) as per manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... All cell lines were tested every 6-12 months either by PCR (LookOut kit, Sigma/Merck) or Mycostrips (Invivogen ...
-
bioRxiv - Plant Biology 2023Quote: ... RNA was isolated using the SpectrumTM Plant Total RNA Kit (Sigma Aldrich, Merck KGaA, Darmstadt, Germany). The material was stored at −70 °C until use.
-
bioRxiv - Plant Biology 2023Quote: ... germinating seeds or rosette leaves using Spectrum™ Plant Total RNA Kit (Merck KgaA, Darmstadt, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from cell pellets using the GenElute™ Bacterial Genomic DNA kit (Merck) as per manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2024Quote: ... gDNA was extracted from neuronal cultures with the GenEluteTM Mammalian Genomic DNA Miniprep Kit (G1N10, Merck). qPCR was performed as described above with 20 ng of gDNA in a final volume of 10 μL ...
-
bioRxiv - Molecular Biology 2024Quote: PLA was performed by using Duolink In Situ Red Starter Kit Mouse/Rabbit (Merck, #DUO92101-1KT) according to the manufacturer’s instructions ...
-
bioRxiv - Biophysics 2021Quote: ... Cells were then incubated for 1 h at room temperature with the monoclonal mouse Anti-Tom20 (1:500, MABT166, Merck & Co., Kenilworth, NJ, USA). The antibody was revealed using Atto 594 goat anti-mouse IgG (1:500 ...
-
bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: mouse monoclonal anti-SARM1 (Chen et al., 2011) (1:5000) and mouse anti-β-actin (Merck, A5316, 1:2000) as a loading control ...
-
bioRxiv - Cancer Biology 2023Quote: ... diluted 1:1000) and vinculin loading control (Cell signalling #4650, diluted 1:1000) in 5% bovine serum albumin (BSA) (Merck, Kenilworth, NJ, United States)/TBS-Tween overnight at 4° C ...
-
bioRxiv - Genetics 2023Quote: ... MAGEA4-Dy550 (1:20, Prof. G. C. Spagnoli, University Hospital of Basel, CH, conjugated) and UTF1-Dy488 (1:20, Merck/Millipore, Cat# MAB4337, conjugated). We used the DylightTM 488 and 550 labeling kits (ThermoFisher ...
-
bioRxiv - Cancer Biology 2024Quote: ... The media was replaced after 24 hours with 1 mL growth media mixed with 1 mL of virus media and 8 μg/mL of Polybrene (Merck Millipore, TR-1003-G). One day later ...
-
bioRxiv - Neuroscience 2020Quote: ... Infected and sham-infected animals were separated into four groups according to the treatment received: 10 μL of intramuscular vitamin B12 (Merck, Kenilworth, NJ; 6.25 mg/ kg) (N = 10 infected - vitamin B12 ...
-
bioRxiv - Cell Biology 2019Quote: ... and resuspended in Lysis Buffer (Tris 10 mM pH 8, 2 mM EDTA) supplemented with Complete protease inhibitor (Roche, Merck, Sigma-Aldrich, l’Isle d’Abeau, France). After three cycles of freezing – thawing ...
-
bioRxiv - Microbiology 2020Quote: 1% w/v low melting agarose (Merck/Sigma-Aldrich, Germany) stock was prepared by suspending the agarose powder in 18% BSW (Dyall-Smith ...
-
bioRxiv - Systems Biology 2020Quote: ... supplied with 1% Protease Inhibitor Cocktail Set III (Merck Chemicals). After an incubation of 30 min at 4 °C ...
-
bioRxiv - Molecular Biology 2020Quote: ... anti-active-ß-catenin (#05-665, 1:500, Merck Millipore), anti-H3K9me2 (#9753S ...
-
bioRxiv - Microbiology 2019Quote: ... was performed with 1 µg/ml DAPI solution (Merck-Millipore) in PBS for 10 min ...
-
bioRxiv - Physiology 2022Quote: ... anti-Smpd3 (Merck clone 14G5.1, 1/2000, Merck, Darmstadt, Germany), and anti-Cyclophilin A (catalog n°51418 ...
-
bioRxiv - Cancer Biology 2022Quote: ... and anti-Actin-HRP (1:10000 A3854, Merck, Darmstadt, Germany). Densitometry analysis were performed by using Image J software.
-
bioRxiv - Developmental Biology 2021Quote: ... onto fibronectin-coated fibronectin-coated plates (1:100, Merck, UK), in 300 µL of N2B27media ...
-
bioRxiv - Developmental Biology 2021Quote: ... Cells were seeded onto fibronectin-coated plates (1:100, Merck) in DPBS during 1h at 37°C and grown in N2B27 media - to promote the differentiation of mESCs into a stage resembling the post-implantation epiblast ...
-
bioRxiv - Neuroscience 2021Quote: ... Rabbit anti-cFos (ABE457, 1:5000, Merck Millipore, Hertfordshire, UK) was diluted in 500 μl 0.1% PBST with 0.2% normal donkey serum (v/v ...